LOCUS       NM_001959                808 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens eukaryotic translation elongation factor 1 beta 2
            (EEF1B2), transcript variant 1, mRNA.
ACCESSION   NM_001959
VERSION     NM_001959.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 808)
  AUTHORS   He,C., Guo,J., Tian,W. and Wong,C.C.L.
  TITLE     Proteogenomics Integrating Novel Junction Peptide Identification
            Strategy Discovers Three Novel Protein Isoforms of Human NHSL1 and
            EEF1B2
  JOURNAL   J Proteome Res 20 (12), 5294-5303 (2021)
   PUBMED   34420305
  REMARK    GeneRIF: Proteogenomics Integrating Novel Junction Peptide
            Identification Strategy Discovers Three Novel Protein Isoforms of
            Human NHSL1 and EEF1B2.
REFERENCE   3  (bases 1 to 808)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 808)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 808)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 808)
  AUTHORS   Hochstrasser,D.F., Frutiger,S., Paquet,N., Bairoch,A., Ravier,F.,
            Pasquali,C., Sanchez,J.C., Tissot,J.D., Bjellqvist,B., Vargas,R. et
            al.
  TITLE     Human liver protein map: a reference database established by
            microsequencing and gel comparison
  JOURNAL   Electrophoresis 13 (12), 992-1001 (1992)
   PUBMED   1286669
REFERENCE   7  (bases 1 to 808)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   8  (bases 1 to 808)
  AUTHORS   Sanders,J., Maassen,J.A., Amons,R. and Moller,W.
  TITLE     Nucleotide sequence of human elongation factor-1 beta cDNA
  JOURNAL   Nucleic Acids Res 19 (16), 4551 (1991)
   PUBMED   1886777
REFERENCE   9  (bases 1 to 808)
  AUTHORS   von der Kammer,H., Klaudiny,J., Zimmer,M. and Scheit,K.H.
  TITLE     Human elongation factor 1 beta: cDNA and derived amino acid
            sequence
  JOURNAL   Biochem Biophys Res Commun 177 (1), 312-317 (1991)
   PUBMED   1710449
REFERENCE   10 (bases 1 to 808)
  AUTHORS   Bec,G., Kerjan,P., Zha,X.D. and Waller,J.P.
  TITLE     Valyl-tRNA synthetase from rabbit liver. I. Purification as a
            heterotypic complex in association with elongation factor 1
  JOURNAL   J Biol Chem 264 (35), 21131-21137 (1989)
   PUBMED   2556394
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from X60489.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001959.3.
            
            Summary: This gene encodes a translation elongation factor. The
            protein is a guanine nucleotide exchange factor involved in the
            transfer of aminoacylated tRNAs to the ribosome. Alternative
            splicing results in three transcript variants which differ only in
            the 5' UTR. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This predominant variant (1) has a distinct 5'
            UTR. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X60489.1, SRR1163655.11650.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392222.7/ ENSP00000376056.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-808               X60489.1           151-958
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.3"
     gene            1..808
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /note="eukaryotic translation elongation factor 1 beta 2"
                     /db_xref="GeneID:1933"
                     /db_xref="HGNC:HGNC:3208"
                     /db_xref="MIM:600655"
     exon            1..165
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..763
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /note="eukaryotic translation elongation factor 1 beta 1;
                     EF-1-beta"
                     /codon_start=1
                     /product="elongation factor 1-beta"
                     /protein_id="NP_001950.1"
                     /db_xref="CCDS:CCDS2367.1"
                     /db_xref="GeneID:1933"
                     /db_xref="HGNC:HGNC:3208"
                     /db_xref="MIM:600655"
                     /translation="MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSP
                     PPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDID
                     LFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEE
                     CVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMD
                     VAAFNKI"
     exon            166..288
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            289..415
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            416..482
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            483..608
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            609..808
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      789..794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /note="hexamer: AATAAA"
     polyA_site      808
                     /gene="EEF1B2"
                     /gene_synonym="EEF1B; EEF1B1; EF1B"
                     /note="major polyA site"
ORIGIN      
        1 cctttttcct ctcttcagcg tggggcgccc acaatttgcg cgctctcttt ctgctgctcc
       61 ccagctctcg gatacagccg acaccatggg tttcggagac ctgaaaagcc ctgccggcct
      121 ccaggtgctc aacgattacc tggcggacaa gagctacatc gaggggtatg tgccatcaca
      181 agcagatgtg gcagtatttg aagccgtgtc cagcccaccg cctgccgact tgtgtcatgc
      241 cctacgttgg tataatcaca tcaagtctta cgaaaaggaa aaggccagcc tgccaggagt
      301 gaagaaagct ttgggcaaat atggtcctgc cgatgtggaa gacactacag gaagtggagc
      361 tacagatagt aaagatgatg atgacattga cctctttgga tctgatgatg aggaggaaag
      421 tgaagaagca aagaggctaa gggaagaacg tcttgcacaa tatgaatcaa agaaagccaa
      481 aaaacctgca cttgttgcca agtcttccat cttactagat gtgaaacctt gggatgatga
      541 gacagatatg gcgaaattag aggagtgcgt cagaagcatt caagcagacg gcttagtctg
      601 gggctcatct aaactagttc cagtgggata cggaattaag aaacttcaaa tacagtgtgt
      661 agttgaagat gataaagttg gaacagatat gctggaggag cagatcactg cttttgagga
      721 ctatgtgcag tccatggatg tggctgcttt caacaagatc taaaatccat cctggatcat
      781 ggcatttaaa taaaagattg aaagatta
//
