LOCUS       NM_032331                975 bp    mRNA    linear   PRI 27-JUL-2024
DEFINITION  Homo sapiens EEF1A lysine methyltransferase 4 (EEF1AKMT4), mRNA.
ACCESSION   NM_032331
VERSION     NM_032331.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Jakobsson,M.E., Malecki,J., Nilges,B.S., Moen,A., Leidel,S.A. and
            Falnes,P.O.
  TITLE     Methylation of human eukaryotic elongation factor alpha (eEF1A) by
            a member of a novel protein lysine methyltransferase family
            modulates mRNA translation
  JOURNAL   Nucleic Acids Res 45 (14), 8239-8254 (2017)
   PUBMED   28520920
  REMARK    GeneRIF: Methylation of lysine (K36) in eukaryotic elongation
            factor alpha (eEF1A) proteins is dependent on EEF1A lysine
            methyltransferase 4 (eEF1A-KMT4) in vivo.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC069005.1 and BC005835.2.
            
            On Nov 30, 2019 this sequence version replaced NM_032331.3.
            
            Summary: This gene encodes a member of the lysine-specific
            methyltransferase (KMT) family. The encoded enzyme catalyzes the
            methylation of lysine-36 of the eukaryotic translation elongation
            factor 1 alpha. Methylation by this enzyme may affect endoplasmic
            reticulum-related processes. [provided by RefSeq, Jul 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005835.2, BX341457.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324557.9/ ENSP00000314295.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-969               BC069005.1         2-970
            970-975             BC005835.2         981-986
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..975
                     /gene="EEF1AKMT4"
                     /note="EEF1A lysine methyltransferase 4"
                     /db_xref="GeneID:110599564"
                     /db_xref="HGNC:HGNC:53611"
     exon            1..219
                     /gene="EEF1AKMT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..791
                     /gene="EEF1AKMT4"
                     /note="eukaryotic translation elongation factor 1 alpha
                     lysine specific methyltransferase 4"
                     /codon_start=1
                     /product="EEF1A lysine methyltransferase 4"
                     /protein_id="NP_115707.2"
                     /db_xref="CCDS:CCDS3255.1"
                     /db_xref="GeneID:110599564"
                     /db_xref="HGNC:HGNC:53611"
                     /translation="MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGD
                     FSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHA
                     HVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSE
                     VSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSLRHATYGSGFHFHLYLMHKGGKLS
                     VAQLALGAQILSPPRPPTSPCFLQDSDHEDFLSAIQL"
     exon            220..503
                     /gene="EEF1AKMT4"
                     /inference="alignment:Splign:2.1.0"
     exon            504..975
                     /gene="EEF1AKMT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      947..952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1AKMT4"
                     /note="hexamer: AATACA"
     polyA_site      975
                     /gene="EEF1AKMT4"
                     /note="major polyA site"
ORIGIN      
        1 ctggctgccc ggcggttgag agcatggcct ctccaggggc aggtagggcg cctccggagt
       61 taccggagcg gaactgcggg taccgcgaag tcgagtactg ggatcagcgc taccaaggcg
      121 cagccgattc tgccccctac gattggttcg gggacttctc ctccttccgt gccctcctag
      181 agccggagct gcggcccgag gaccgtatcc ttgtgctagg ttgcgggaac agtgccctga
      241 gctacgagct gttcctcgga ggcttcccta atgtgaccag tgtggactac tcatcagtcg
      301 tggtggctgc catgcaggct cgccatgccc atgtgccgca gctgcgctgg gagaccatgg
      361 atgtgcggaa gctggacttc cccagtgctt cttttgatgt ggtgctcgag aagggcacgc
      421 tggatgccct gctggctggg gaacgagatc cctggaccgt gtcctctgaa ggtgtccaca
      481 ctgtggacca ggtgttgagt gaggtgagcc gcgtgcttgt ccctggaggc cggtttatct
      541 caatgacttc tgctgccccc cactttcgga ccagacacta tgcccaagcc tattatggct
      601 ggtccctgag gcatgctacc tatggcagcg gtttccactt ccatctctac ctcatgcaca
      661 agggcgggaa gctcagtgtg gcccagctgg ctctgggggc ccaaatcctc tcacccccca
      721 gacctcccac ctcaccttgc ttccttcagg actcagatca tgaggacttc cttagtgcca
      781 ttcagctctg aggccagagc atggtcctcc acccttcctg ccattctgcc ctgggctcct
      841 caggtagttg gaattcctga cttaggactt ggggttgggt ccaaggtgct tacatcccag
      901 gggcctcatg cctaagatag agggtgggag cgaacccaca tgaaccaata cagcccagct
      961 ccaactagat ccaga
//
