LOCUS       NM_014693               3468 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.
ACCESSION   NM_014693
VERSION     NM_014693.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Jakobsson,M.E., Malecki,J., Nilges,B.S., Moen,A., Leidel,S.A. and
            Falnes,P.O.
  TITLE     Methylation of human eukaryotic elongation factor alpha (eEF1A) by
            a member of a novel protein lysine methyltransferase family
            modulates mRNA translation
  JOURNAL   Nucleic Acids Res 45 (14), 8239-8254 (2017)
   PUBMED   28520920
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   3  (bases 1 to 3468)
  AUTHORS   Lorenzo,M.N., Khan,R.Y., Wang,Y., Tai,S.C., Chan,G.C., Cheung,A.H.
            and Marsden,P.A.
  TITLE     Human endothelin converting enzyme-2 (ECE2): characterization of
            mRNA species and chromosomal localization
  JOURNAL   Biochim Biophys Acta 1522 (1), 46-52 (2001)
   PUBMED   11718899
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC061705.16 and AC078797.20.
            
            On Jun 2, 2019 this sequence version replaced NM_014693.3.
            
            Summary: This gene represents naturally occurring readthrough
            transcription between adjacent genes eukaryotic translation
            elongation factor 1 alpha lysine methyltransferase 4 (GeneID:
            110599564) and endothelin converting enzyme 2 (GeneID:9718). The
            readthrough transcript representing this gene encodes a fusion
            protein that shares sequence identity with each individual gene
            product. [provided by RefSeq, Jul 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF428263.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 9718,
                                      110599564
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AC061705.16        10746-10964         c
            220-503             AC061705.16        2880-3163           c
            504-639             AC078797.20        18257-18392
            640-855             AC078797.20        18616-18831
            856-980             AC078797.20        19290-19414
            981-1127            AC078797.20        19532-19678
            1128-1193           AC078797.20        19857-19922
            1194-1382           AC078797.20        25150-25338
            1383-1525           AC078797.20        26328-26470
            1526-1640           AC078797.20        26843-26957
            1641-1751           AC078797.20        29202-29312
            1752-1850           AC078797.20        30802-30900
            1851-1928           AC078797.20        31006-31083
            1929-2032           AC078797.20        31620-31723
            2033-2143           AC078797.20        31922-32032
            2144-2211           AC078797.20        32158-32225
            2212-2402           AC078797.20        32405-32595
            2403-2498           AC078797.20        32709-32804
            2499-3468           AC078797.20        33427-34396
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..3468
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /note="EEF1AKMT4-ECE2 readthrough"
                     /db_xref="GeneID:110599583"
                     /db_xref="HGNC:HGNC:53615"
     exon            1..219
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..2675
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /EC_number="3.4.24.71"
                     /note="Endothelin-converting enzyme 2"
                     /codon_start=1
                     /product="EEF1AKMT4-ECE2 readthrough transcript protein"
                     /protein_id="NP_055508.3"
                     /db_xref="GeneID:110599583"
                     /db_xref="HGNC:HGNC:53615"
                     /translation="MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGD
                     FSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHA
                     HVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSE
                     VGFQKGTRQLLGSRTQLELVLAGASLLLAALLLGCLVALGVQYHRDPSHSTCLTEACI
                     RVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHL
                     LENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNF
                     MEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVL
                     TAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSIS
                     ELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLI
                     WNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVK
                     ATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFIL
                     EPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYY
                     LPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLR
                     PWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAW
                     LRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTL
                     SNSRDFLRHFGCPVGSPMNPGQLCEVW"
     exon            220..503
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            504..639
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            640..855
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            856..980
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1127
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1193
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1382
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1525
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1640
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1751
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1850
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1928
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2032
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2143
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2211
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2402
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2498
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..3468
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3446..3451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /note="hexamer: AATAAA"
     polyA_site      3468
                     /gene="EEF1AKMT4-ECE2"
                     /gene_synonym="ECE-2; ECE2"
                     /note="major polyA site"
ORIGIN      
        1 ctggctgccc ggcggttgag agcatggcct ctccaggggc aggtagggcg cctccggagt
       61 taccggagcg gaactgcggg taccgcgaag tcgagtactg ggatcagcgc taccaaggcg
      121 cagccgattc tgccccctac gattggttcg gggacttctc ctccttccgt gccctcctag
      181 agccggagct gcggcccgag gaccgtatcc ttgtgctagg ttgcgggaac agtgccctga
      241 gctacgagct gttcctcgga ggcttcccta atgtgaccag tgtggactac tcatcagtcg
      301 tggtggctgc catgcaggct cgccatgccc atgtgccgca gctgcgctgg gagaccatgg
      361 atgtgcggaa gctggacttc cccagtgctt cttttgatgt ggtgctcgag aagggcacgc
      421 tggatgccct gctggctggg gaacgagatc cctggaccgt gtcctctgaa ggtgtccaca
      481 ctgtggacca ggtgttgagt gaggtgggat tccagaaggg gacaagacag ctgttaggct
      541 cacgcacgca gctggagctg gtcttagcag gtgcctctct actgctggct gcactgcttc
      601 tgggctgcct tgtggcccta ggggtccagt accacagaga cccatcccac agcacctgcc
      661 ttacagaggc ctgcattcga gtggctggaa aaatcctgga gtccctggac cgaggggtga
      721 gcccctgtga ggacttttac cagttctcct gtgggggctg gattcggagg aaccccctgc
      781 ccgatgggcg ttctcgctgg aacaccttca acagcctctg ggaccaaaac caggccatac
      841 tgaagcacct gcttgaaaac accaccttca actccagcag tgaagctgag cagaagacac
      901 agcgcttcta cctatcttgc ctacaggtgg agcgcattga ggagctggga gcccagccac
      961 tgagagacct cattgagaag attggtggtt ggaacattac ggggccctgg gaccaggaca
     1021 actttatgga ggtgttgaag gcagtagcag ggacctacag ggccacccca ttcttcaccg
     1081 tctacatcag tgccgactct aagagttcca acagcaatgt tatccaggtg gaccagtctg
     1141 ggctctttct gccctctcgg gattactact taaacagaac tgccaatgag aaagtgctca
     1201 ctgcctatct ggattacatg gaggaactgg ggatgctgct gggtgggcgg cccacctcca
     1261 cgagggagca gatgcagcag gtgctggagt tggagataca gctggccaac atcacagtgc
     1321 cccaggacca gcggcgcgac gaggagaaga tctaccacaa gatgagcatt tcggagctgc
     1381 aggctctggc gccctccatg gactggcttg agttcctgtc tttcttgctg tcaccattgg
     1441 agttgagtga ctctgagcct gtggtggtgt atgggatgga ttatttgcag caggtgtcag
     1501 agctcatcaa ccgcacggaa ccaagcatcc tgaacaatta cctgatctgg aacctggtgc
     1561 aaaagacaac ctcaagcctg gaccgacgct ttgagtctgc acaagagaag ctgctggaga
     1621 ccctctatgg cactaagaag tcctgtgtgc cgaggtggca gacctgcatc tccaacacgg
     1681 atgacgccct tggctttgct ttggggtccc tcttcgtgaa ggccacgttt gaccggcaaa
     1741 gcaaagaaat tgcagagggg atgatcagcg aaatccggac cgcatttgag gaggccctgg
     1801 gacagctggt ttggatggat gagaagaccc gccaggcagc caaggagaaa gcagatgcca
     1861 tctatgatat gattggtttc ccagacttta tcctggagcc caaagagctg gatgatgttt
     1921 atgacgggta cgaaatttct gaagattctt tcttccaaaa catgttgaat ttgtacaact
     1981 tctctgccaa ggttatggct gaccagctcc gcaagcctcc cagccgagac cagtggagca
     2041 tgacccccca gacagtgaat gcctactacc ttccaactaa gaatgagatc gtcttccccg
     2101 ctggcatcct gcaggccccc ttctatgccc gcaaccaccc caaggccctg aacttcggtg
     2161 gcatcggtgt ggtcatgggc catgagttga cgcatgcctt tgatgaccaa gggcgcgagt
     2221 atgacaaaga agggaacctg cggccctggt ggcagaatga gtccctggca gccttccgga
     2281 accacacggc ctgcatggag gaacagtaca atcaatacca ggtcaatggg gagaggctca
     2341 acggccgcca gacgctgggg gagaacattg ctgacaacgg ggggctgaag gctgcctaca
     2401 atgcttacaa agcatggctg agaaagcatg gggaggagca gcaactgcca gccgtggggc
     2461 tcaccaacca ccagctcttc ttcgtgggat ttgcccaggt gtggtgctcg gtccgcacac
     2521 cagagagctc tcacgagggg ctggtgaccg acccccacag ccctgcccgc ttccgcgtgc
     2581 tgggcactct ctccaactcc cgtgacttcc tgcggcactt cggctgccct gtcggctccc
     2641 ccatgaaccc agggcagctg tgtgaggtgt ggtagacctg gatcagggga gaaatggcca
     2701 gctgtcacca gacctggggc agctctcctg acaaagctgt ttgctcttgg gttgggagga
     2761 agcaaatgca agctgggctg ggtctagtcc ctccccccca caggtgacat gagtacagac
     2821 cctcctcaat caccacattg tgcctctgct ttgggggtgc ccctgcctcc agcagagccc
     2881 ccaccattca ctgtgacatc tttccgtgtc accctgcctg gaagaggtct gggtggggag
     2941 gccagttccc ataggaagga gtctgcctct tctgtcccca ggctcactca gcctggcggc
     3001 catggggcct gccgtgcctg ccccactgtg acccacaggc ctgggtggtg tacctcctgg
     3061 acttctcccc aggctcactc agtgcgcact taggggtgga ctcagctctg tctggctcac
     3121 cctcacgggc tacccccacc tcaccctgtg ctccttgtgc cactgctccc agtgctgctg
     3181 ctgaccttca ctgacagctc ctagtggaag cccaagggcc tctgaaagcc tcctgctgcc
     3241 cactgtttcc ctgggctgag aggggaagtg catatgtgta gcgggtactg gttcctgtgt
     3301 cttagggcac aagccttagc aaatgattga ttctccctgg acaaagcagg aaagcagata
     3361 gagcagggaa aaggaagaac agagtttatt tttacagaaa agagggtggg agggtgtggt
     3421 cttggccctt ataggaccct gtgccaataa acagacatgc atccgtca
//
