LOCUS       NM_015433               2687 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3),
            transcript variant 1, mRNA.
ACCESSION   NM_015433
VERSION     NM_015433.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Shu,X., Li,X., Xiang,X., Wang,Q. and Wu,Q.
  TITLE     METTL21B is a prognostic biomarker and potential therapeutic target
            in low-grade gliomas
  JOURNAL   Aging (Albany NY) 13 (16), 20661-20683 (2021)
   PUBMED   34446611
  REMARK    GeneRIF: METTL21B is a prognostic biomarker and potential
            therapeutic target in low-grade gliomas.
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2687)
  AUTHORS   Hamey,J.J., Wienert,B., Quinlan,K.G.R. and Wilkins,M.R.
  TITLE     METTL21B Is a Novel Human Lysine Methyltransferase of Translation
            Elongation Factor 1A: Discovery by CRISPR/Cas9 Knockout
  JOURNAL   Mol Cell Proteomics 16 (12), 2229-2242 (2017)
   PUBMED   28663172
  REMARK    GeneRIF: Results have shown that METTL21B is a protein
            methyltransferase that methylates both isoforms of translation
            elongation factor eEF1A at lysine 165. Also, further data suggests
            that METTL21B has an evolutionarily important role for the
            methylation of lysine 165 in eEF1A.
REFERENCE   4  (bases 1 to 2687)
  AUTHORS   Malecki,J., Aileni,V.K., Ho,A.Y.Y., Schwarz,J., Moen,A.,
            Sorensen,V., Nilges,B.S., Jakobsson,M.E., Leidel,S.A. and
            Falnes,P.O.
  TITLE     The novel lysine specific methyltransferase METTL21B affects mRNA
            translation through inducible and dynamic methylation of Lys-165 in
            human eukaryotic elongation factor 1 alpha (eEF1A)
  JOURNAL   Nucleic Acids Res 45 (8), 4370-4389 (2017)
   PUBMED   28108655
  REMARK    GeneRIF: The present study identifies METTL21B as the enzyme
            responsible for methylation of eEF1A on Lys-165 and shows that this
            modification is dynamic, inducible and likely of regulatory
            importance.
REFERENCE   5  (bases 1 to 2687)
  AUTHORS   Cloutier,P., Lavallee-Adam,M., Faubert,D., Blanchette,M. and
            Coulombe,B.
  TITLE     A newly uncovered group of distantly related lysine
            methyltransferases preferentially interact with molecular
            chaperones to regulate their activity
  JOURNAL   PLoS Genet 9 (1), e1003210 (2013)
   PUBMED   23349634
REFERENCE   6  (bases 1 to 2687)
  AUTHORS   Kernstock,S., Davydova,E., Jakobsson,M., Moen,A., Pettersen,S.,
            Maelandsmo,G.M., Egge-Jacobsen,W. and Falnes,P.O.
  TITLE     Lysine methylation of VCP by a member of a novel human protein
            methyltransferase family
  JOURNAL   Nat Commun 3, 1038 (2012)
   PUBMED   22948820
  REMARK    GeneRIF: The METTL21B protein is here suggested to be a protein
            methyltransferase, as it is shown to belong to a family of 10 human
            methyltransferases, some of which are shown to have lysine specific
            protein methyltransferase activity.
REFERENCE   7  (bases 1 to 2687)
  AUTHORS   Gandhi,K.S., McKay,F.C., Cox,M., Riveros,C., Armstrong,N.,
            Heard,R.N., Vucic,S., Williams,D.W., Stankovich,J., Brown,M.,
            Danoy,P., Stewart,G.J., Broadley,S., Moscato,P., Lechner-Scott,J.,
            Scott,R.J. and Booth,D.R.
  CONSRTM   ANZgene Multiple Sclerosis Genetics Consortium
  TITLE     The multiple sclerosis whole blood mRNA transcriptome and genetic
            associations indicate dysregulation of specific T cell pathways in
            pathogenesis
  JOURNAL   Hum Mol Genet 19 (11), 2134-2143 (2010)
   PUBMED   20190274
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 2687)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC016395.1 and AL050100.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015433.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA938041.1, SRR1803611.110587.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300209.13/ ENSP00000300209.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2549              BC016395.1         15-2563
            2550-2687           AL050100.1         2624-2761
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14.1"
     gene            1..2687
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /note="EEF1A lysine methyltransferase 3"
                     /db_xref="GeneID:25895"
                     /db_xref="HGNC:HGNC:24936"
                     /db_xref="MIM:615258"
     exon            1..288
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..792
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /note="isoform a is encoded by transcript variant 1;
                     family with sequence similarity 119, member B;
                     hepatocellularcarcinoma-associated antigen HCA557a;
                     methyltransferase-like protein 21B; eEF1A-KMT3;
                     hepatocellular carcinoma-associated antigen 557a;
                     methyltransferase like 21B; protein-lysine
                     methyltransferase METTL21B"
                     /codon_start=1
                     /product="EEF1A lysine methyltransferase 3 isoform a"
                     /protein_id="NP_056248.2"
                     /db_xref="CCDS:CCDS8957.1"
                     /db_xref="GeneID:25895"
                     /db_xref="HGNC:HGNC:24936"
                     /db_xref="MIM:615258"
                     /translation="MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQ
                     NFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGGDVTI
                     TDLPLALEQIQGNVQANVPAGGQAQVRALSWGIDHHVFPANYDLVLGADIVYLEPTFP
                     LLLGTLQHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDENVNIYRA
                     RHREPRPA"
     exon            289..400
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /inference="alignment:Splign:2.1.0"
     exon            401..2687
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2664..2669
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /note="hexamer: AATAAA"
     polyA_site      2687
                     /gene="EEF1AKMT3"
                     /gene_synonym="FAM119B; METTL21B"
                     /note="major polyA site"
ORIGIN      
        1 gctcgttcca cagggacacc acgacggctc gcggccccca gcctctaccc cgctccggat
       61 ccgggatctg agcgccggcc gcggtgccca ggcactccct tggcgggccg gatggcggac
      121 cccggcccag atcccgaatc tgagtcggaa tcggtgttcc cgcgggaggt cgggctcttt
      181 gcagactctt actcggagaa gagccagttc tgtttctgtg ggcatgtgct gaccatcacg
      241 cagaactttg ggtcccgcct cggggtggcg gcgcgcgtgt gggacgcggc cctgagcctg
      301 tgcaattatt tcgagagtca aaatgtggat ttccgaggca agaaggtgat cgaactgggt
      361 gcggggacag gcatcgtggg gatcttggca gcgctgcagg ggggggatgt taccatcact
      421 gacctgcccc tggccctaga acagatccag ggcaacgtcc aggccaatgt gccagctgga
      481 ggccaggccc aggtgcgtgc cttgtcctgg gggattgacc atcatgtctt ccctgcaaac
      541 tatgacctgg tgctgggggc tgatatcgtg tacctggaac ccaccttccc tctgctgctg
      601 gggaccctcc aacacctgtg caggccccat ggcaccatct atctggcctc caagatgaga
      661 aaggagcatg ggacagagag cttctttcag cacctcctgc cccagcattt ccaactggag
      721 ctggctcagc gggatgagga tgaaaatgtc aacatctata gggccaggca cagggaacca
      781 agacctgctt gacatcaccc ttgctgttct tctcaatctc ttgctctaat gaaggaactg
      841 tgtatctcaa aaaccatatt tccagagcca caaacatgag gaccaaaaag gatggatttc
      901 cctggcctct ctcactttcc tccttccctc tttgttaccc cagatactct tgggcaggtg
      961 cagtgaggtg cctgcttaca aggaatccca gagttctggc agcactagtg ttagaacacc
     1021 aaggaggttc ctggctcctg ttctcagaga aggaggatgg taaggtatcc aggattttta
     1081 gggggtaagg gagatatatg ggatgtgaga gcagtgattg tagacagact gtcactgatc
     1141 acttccattc ctgttgctgt ttacacagaa tggactttaa aaaaattctg tgggattttt
     1201 ttttcttttt ttcttttttt ttgagacagg gtctcactct gtcatccagg ctggagtgca
     1261 gtggtgtgat cacagctcac tgcagcctca acctctgaag gcccagatga tcttcctgcc
     1321 tcagctcccc aagtagctgg gactagaggc atgcgccacc acacccggct aatttttgta
     1381 ttttcagtag agacagggtt tcgccatgtt gcccagggtg gtctcaaact cctgggctca
     1441 agtgatccac ccgccttgcc tcccaaagtg ctgggattgc aggcatggcc cactgtgcta
     1501 gcctaggatt tttttttttt tttaagaaag aattaagcct taaaaatcta ttgtgcaaga
     1561 acccaacttt tactcaggag ctgagaagga agaagcctca aaccacccat ttcctgcttt
     1621 ttagagatag agtctagtgg aaccatggct ctaactgggt caagtcctcc tagctctgaa
     1681 gctaaggctg cagagctgtc ctttggactg tatttcttca tgctttccag agttagagcc
     1741 ttgatcacgg aacatcattg tgtgcagaag aatactgtac ctagtactaa gttcttttca
     1801 tctttgattt ttctttagct tcatcagtcc ttccttccta atctctaatc ccctgaagcc
     1861 taacacagtt cctaatgtgt tttgccttaa cctcttttct cttcccacat tggtacttct
     1921 ggttggggct ctctttactt actgcttagg cacatagtca ccttctctgg actccctgct
     1981 tccatgcttt ctccttcagt ccattccgca aactcttgtg atcttccaaa attagctctg
     2041 attagctttc acttcctgta gaagcaagaa caagctcctc tgcttggtac ttagtctgtg
     2101 tccatgtgat ccaaaattac cttcctattg catttcctac tcctttgcta cgtgtattgg
     2161 acctctggcc aaattacaaa agatgtactc tttgttctct aaaaccacac taaagttttc
     2221 tgacttcttt cacactcttt atgataatcc tctgcctgaa aggctattgc cacctgtcaa
     2281 aatcttcatt attctttgcc tctttcctga gcctcttgct tgaatgtgat ttctttctcc
     2341 ctgagaccca taaggttcat cttatgggac cttaatttgt cttgcatgga catttgtgat
     2401 tctcattttc tcaccattaa attaccttgg agggtagggc tgtggtttac tcctgtctaa
     2461 acccctgaag cctaacacag ttcctggcac acaacaggtg ttcaacagat attgtgctca
     2521 ttcaataaat atttactcgc tttgtttaat ggattccacc cctgccttcc taggtgacct
     2581 tggacaaatt ctcgaaccca ttcaccaacc acacaagttt gttgtgagtc ataaatggat
     2641 aatgaatata aatgcgcatt gaaaataaac ataaaatgtt acccaca
//
