LOCUS       NM_001956               1253 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.
ACCESSION   NM_001956
VERSION     NM_001956.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Suprapto,R.P., Suzuki,Y., Nagano,T., Hirata,K.I. and Emoto,N.
  TITLE     The loss of endothelin-2 exhibits an anticancer effect in A549
            human lung adenocarcinoma cell line
  JOURNAL   Can J Physiol Pharmacol 100 (8), 818-827 (2022)
   PUBMED   35679618
  REMARK    GeneRIF: The loss of endothelin-2 exhibits an anticancer effect in
            A549 human lung adenocarcinoma cell line.
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Szymanska,M., Shrestha,K., Girsh,E., Harlev,A., Eisenberg,I.,
            Imbar,T. and Meidan,R.
  TITLE     Reduced Endothelin-2 and Hypoxic Signaling Pathways in
            Granulosa-Lutein Cells of PCOS Women
  JOURNAL   Int J Mol Sci 22 (15), 8216 (2021)
   PUBMED   34360981
  REMARK    GeneRIF: Reduced Endothelin-2 and Hypoxic Signaling Pathways in
            Granulosa-Lutein Cells of PCOS Women.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1253)
  AUTHORS   Buyukates,M., Barut,F. and Aktunc,E.
  TITLE     The comparison of traditional and modified harvesting techniques of
            left internal mammary artery regarding endothelin-1/2/3 expression
            and free flow capacity
  JOURNAL   J Cardiovasc Surg (Torino) 56 (6), 913-918 (2015)
   PUBMED   24647318
  REMARK    GeneRIF: EDN2 expression in left internal mammary arteries depends
            on tissue harvesting technique.
REFERENCE   4  (bases 1 to 1253)
  AUTHORS   Cacioppo,J.A., Oh,S.W., Kim,H.Y., Cho,J., Lin,P.C., Yanagisawa,M.
            and Ko,C.
  TITLE     Loss of function of endothelin-2 leads to reduced ovulation and CL
            formation
  JOURNAL   PLoS One 9 (4), e96115 (2014)
   PUBMED   24763822
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1253)
  AUTHORS   Rasmussen,P.H., Plum,I.G., Bruun,N.E., Dige-Petersen,H., Hedner,T.,
            Hedner,J. and Giese,J.
  TITLE     Radioimmunoassay of endothelin in human plasma
  JOURNAL   Blood Press 1 (3), 181-186 (1992)
   PUBMED   1345052
REFERENCE   6  (bases 1 to 1253)
  AUTHORS   Marciniak,S.J., Plumpton,C., Barker,P.J., Huskisson,N.S. and
            Davenport,A.P.
  TITLE     Localization of immunoreactive endothelin and proendothelin in the
            human lung
  JOURNAL   Pulm Pharmacol 5 (3), 175-182 (1992)
   PUBMED   1446139
REFERENCE   7  (bases 1 to 1253)
  AUTHORS   Poli,E., Barboso,G., Casoli,C., Starcich,R. and Bertaccini,G.
  TITLE     Positive inotropic effect of endothelin-2 on human atrium
            preparations in vitro
  JOURNAL   Cardioscience 2 (2), 99-104 (1991)
   PUBMED   1652300
REFERENCE   8  (bases 1 to 1253)
  AUTHORS   Onda,H., Ohkubo,S., Kosaka,T., Yasuhara,T., Ogi,K., Hosoya,M.,
            Matsumoto,H., Suzuki,N., Kitada,C., Ishibashi,Y. et al.
  TITLE     Expression of endothelin-2 (ET-2) gene in a human renal
            adenocarcinoma cell line: purification and cDNA cloning of ET-2
  JOURNAL   J Cardiovasc Pharmacol 17 Suppl 7, S39-S43 (1991)
   PUBMED   1725387
REFERENCE   9  (bases 1 to 1253)
  AUTHORS   McKay,K.O., Black,J.L., Diment,L.M. and Armour,C.L.
  TITLE     Functional and autoradiographic studies of endothelin-1 and
            endothelin-2 in human bronchi, pulmonary arteries, and airway
            parasympathetic ganglia
  JOURNAL   J Cardiovasc Pharmacol 17 Suppl 7, S206-S209 (1991)
   PUBMED   1725334
REFERENCE   10 (bases 1 to 1253)
  AUTHORS   Ohkubo,S., Ogi,K., Hosoya,M., Matsumoto,H., Suzuki,N., Kimura,C.,
            Ondo,H. and Fujino,M.
  TITLE     Specific expression of human endothelin-2 (ET-2) gene in a renal
            adenocarcinoma cell line. Molecular cloning of cDNA encoding the
            precursor of ET-2 and its characterization
  JOURNAL   FEBS Lett 274 (1-2), 136-140 (1990)
   PUBMED   1701397
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY177176.1, BC034393.1 and
            AW452560.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001956.4.
            
            Summary: This gene encodes a member of the endothelin protein
            family of secretory vasoconstrictive peptides. The preproprotein is
            processed to a short mature form which functions as a ligand for
            the endothelin receptors that initiate intracellular signaling
            events. This gene product is involved in a wide range of biological
            processes, such as hypertension and ovulation. Altered expression
            of this gene is implicated in tumorigenesis. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Oct
            2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034393.1, M65199.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372587.5/ ENSP00000361668.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                HY177176.1         2-58
            58-1252             BC034393.1         1-1195
            1253-1253           AW452560.1         15-15               c
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.2"
     gene            1..1253
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /note="endothelin 2"
                     /db_xref="GeneID:1907"
                     /db_xref="HGNC:HGNC:3177"
                     /db_xref="MIM:131241"
     exon            1..146
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..619
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; preproendothelin 2"
                     /codon_start=1
                     /product="endothelin-2 isoform 1 preproprotein"
                     /protein_id="NP_001947.1"
                     /db_xref="CCDS:CCDS462.1"
                     /db_xref="GeneID:1907"
                     /db_xref="HGNC:HGNC:3177"
                     /db_xref="MIM:131241"
                     /translation="MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHL
                     RLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSAR
                     DPACATFCLRRPWTEAGAVPSRKSPADVFQTGKTGATTGELLQRLRDISTVKSLFAKR
                     QQEAMREPRSTHSRWRKR"
     exon            147..303
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /inference="alignment:Splign:2.1.0"
     exon            304..426
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /inference="alignment:Splign:2.1.0"
     exon            427..525
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..1253
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1236..1241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /note="hexamer: AATAAA"
     polyA_site      1253
                     /gene="EDN2"
                     /gene_synonym="ET-2; ET2; PPET2"
                     /note="major polyA site"
ORIGIN      
        1 gagctatctg ctcagcttaa tagcaggacg ctggcaacag gcactccctg ctccagtcca
       61 gcctgcgcgc tccaccgccg ctatggtctc cgtgcctacc acctggtgct ccgttgcgct
      121 agccctgctc gtggccctgc atgaagggaa gggccaggct gctgccaccc tggagcagcc
      181 agcgtcctca tctcatgccc aaggcaccca ccttcggctt cgccgttgct cctgcagctc
      241 ctggctcgac aaggagtgcg tctacttctg ccacttggac atcatctggg tgaacactcc
      301 tgaacagaca gctccttacg gcctgggaaa cccgccaaga cgccggcgcc gctccctgcc
      361 aaggcgctgt cagtgctcca gtgccaggga ccccgcctgt gccaccttct gccttcgaag
      421 gccctggact gaagccgggg cagtcccaag ccggaagtcc cctgcagacg tgttccagac
      481 tggcaagaca ggggccacta caggagagct tctccaaagg ctgagggaca tttccacagt
      541 caagagcctc tttgccaagc gacaacagga ggccatgcgg gagcctcggt ccacacattc
      601 caggtggagg aagagatagt gtcgtgagct ggaggaacat tgggaaggaa gcccgcgggg
      661 agagaggagg agagaagtgg ccagggcttg tggactctct gcctgcttcc tggaccgggg
      721 ccttggtccc agacagctgg acccatttgc caggattggc acaagctccc tggtgaggga
      781 gcctcgtcca aggcagttct gtgtcctcgc actgcccagg gaagccctcg gcctccagac
      841 tgcggagcag cctccagtgc tggctgctgg cccacagctc tgctggaaga actgcatggg
      901 gagtacattc atctggaggc tgcgtcctga ggagtgtcct gtctgctggg ctacaaacca
      961 ggagcaaccg tgcagccacg aacacgcatg cctcagccag ccctggaggc tggatggctc
     1021 ccctgaggct ggcatcctgg ctggctgtgt cctctccagc tttccctccc cagagttctt
     1081 gcaccctcat tccctcggga ccctcccagt gagaagggcc tgctctgctt ttcctgtctg
     1141 tatataactt atttgcccta agaactttga gaatcccaat tatttatttt aatgtatttt
     1201 ttagaccctc tatttacctg cgaacttgtg tttataataa atgaggaaac ata
//
