LOCUS       NM_025191               6837 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ER degradation enhancing alpha-mannosidase like
            protein 3 (EDEM3), transcript variant 2, mRNA.
ACCESSION   NM_025191 NM_017992
VERSION     NM_025191.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Scott,E., Garnham,R., Cheung,K., Duxfield,A., Elliott,D.J. and
            Munkley,J.
  TITLE     Pro-Survival Factor EDEM3 Confers Therapy Resistance in Prostate
            Cancer
  JOURNAL   Int J Mol Sci 23 (15), 8184 (2022)
   PUBMED   35897761
  REMARK    GeneRIF: Pro-Survival Factor EDEM3 Confers Therapy Resistance in
            Prostate Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   George,G., Ninagawa,S., Yagi,H., Furukawa,J.I., Hashii,N.,
            Ishii-Watabe,A., Deng,Y., Matsushita,K., Ishikawa,T., Mamahit,Y.P.,
            Maki,Y., Kajihara,Y., Kato,K., Okada,T. and Mori,K.
  TITLE     Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide
            structures from M8B in mammalian glycoprotein ERAD
  JOURNAL   Elife 10, e70357 (2021)
   PUBMED   34698634
  REMARK    GeneRIF: Purified EDEM3 or EDEM1 alone produces determinant
            oligosaccharide structures from M8B in mammalian glycoprotein ERAD.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6837)
  AUTHORS   Polla,D.L., Edmondson,A.C., Duvet,S., March,M.E., Sousa,A.B.,
            Lehman,A., Niyazov,D., van Dijk,F., Demirdas,S., van
            Slegtenhorst,M.A., Kievit,A.J.A., Schulz,C., Armstrong,L., Bi,X.,
            Rader,D.J., Izumi,K., Zackai,E.H., de Franco,E., Jorge,P.,
            Huffels,S.C., Hommersom,M., Ellard,S., Lefeber,D.J., Santani,A.,
            Hand,N.J., van Bokhoven,H., He,M. and de Brouwer,A.P.M.
  CONSRTM   CAUSES Study
  TITLE     Bi-allelic variants in the ER quality-control mannosidase gene
            EDEM3 cause a congenital disorder of glycosylation
  JOURNAL   Am J Hum Genet 108 (7), 1342-1349 (2021)
   PUBMED   34143952
  REMARK    GeneRIF: Bi-allelic variants in the ER quality-control mannosidase
            gene EDEM3 cause a congenital disorder of glycosylation.
REFERENCE   4  (bases 1 to 6837)
  AUTHORS   Manica,G., Ghenea,S., Munteanu,C.V.A., Martin,E.C., Butnaru,C.,
            Surleac,M., Chiritoiu,G.N., Alexandru,P.R., Petrescu,A.J. and
            Petrescu,S.M.
  TITLE     EDEM3 Domains Cooperate to Perform Its Overall Cell Functioning
  JOURNAL   Int J Mol Sci 22 (4), 2172 (2021)
   PUBMED   33671632
  REMARK    GeneRIF: EDEM3 Domains Cooperate to Perform Its Overall Cell
            Functioning.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6837)
  AUTHORS   Kofanova,O., Bellora,C., Quesada,R.A., Bulla,A.,
            Panadero-Fajardo,S., Keipes,M., Shea,K., Stone,M., Lescuyer,P. and
            Betsou,F.
  TITLE     IL8 and EDEM3 gene expression ratio indicates peripheral blood
            mononuclear cell (PBMC) quality
  JOURNAL   J Immunol Methods 465, 13-19 (2019)
   PUBMED   30496732
  REMARK    GeneRIF: IL8 and EDEM3 gene expression ratio indicates peripheral
            blood mononuclear cell (PBMC) quality.
            Erratum:[J Immunol Methods. 2020 Mar;478:112733. doi:
            10.1016/j.jim.2019.112733.. PMID: 31916985]
REFERENCE   6  (bases 1 to 6837)
  AUTHORS   Olivari,S. and Molinari,M.
  TITLE     Glycoprotein folding and the role of EDEM1, EDEM2 and EDEM3 in
            degradation of folding-defective glycoproteins
  JOURNAL   FEBS Lett 581 (19), 3658-3664 (2007)
   PUBMED   17499246
  REMARK    Review article
REFERENCE   7  (bases 1 to 6837)
  AUTHORS   Hirao,K., Natsuka,Y., Tamura,T., Wada,I., Morito,D., Natsuka,S.,
            Romero,P., Sleno,B., Tremblay,L.O., Herscovics,A., Nagata,K. and
            Hosokawa,N.
  TITLE     EDEM3, a soluble EDEM homolog, enhances glycoprotein endoplasmic
            reticulum-associated degradation and mannose trimming
  JOURNAL   J Biol Chem 281 (14), 9650-9658 (2006)
   PUBMED   16431915
REFERENCE   8  (bases 1 to 6837)
  AUTHORS   Mast,S.W., Diekman,K., Karaveg,K., Davis,A., Sifers,R.N. and
            Moremen,K.W.
  TITLE     Human EDEM2, a novel homolog of family 47 glycosidases, is involved
            in ER-associated degradation of glycoproteins
  JOURNAL   Glycobiology 15 (4), 421-436 (2005)
   PUBMED   15537790
  REMARK    Erratum:[Glycobiology. 2005 May;15(5):13G]
REFERENCE   9  (bases 1 to 6837)
  AUTHORS   Olivari,S., Galli,C., Alanen,H., Ruddock,L. and Molinari,M.
  TITLE     A novel stress-induced EDEM variant regulating endoplasmic
            reticulum-associated glycoprotein degradation
  JOURNAL   J Biol Chem 280 (4), 2424-2428 (2005)
   PUBMED   15579471
REFERENCE   10 (bases 1 to 6837)
  AUTHORS   Sood,R., Bonner,T.I., Makalowska,I., Stephan,D.A., Robbins,C.M.,
            Connors,T.D., Morgenbesser,S.D., Su,K., Faruque,M.U., Pinkett,H.,
            Graham,C., Baxevanis,A.D., Klinger,K.W., Landes,G.M., Trent,J.M.
            and Carpten,J.D.
  TITLE     Cloning and characterization of 13 novel transcripts and the human
            RGS8 gene from the 1q25 region encompassing the hereditary prostate
            cancer (HPC1) locus
  JOURNAL   Genomics 73 (2), 211-222 (2001)
   PUBMED   11318611
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA724785.1, AF288393.1, BC016464.1, AI521228.1 and AL096819.17.
            
            On Nov 23, 2018 this sequence version replaced NM_025191.3.
            
            Summary: Quality control in the endoplasmic reticulum (ER) ensures
            that only properly folded proteins are retained in the cell through
            recognition and degradation of misfolded or unassembled proteins.
            EDEM3 belongs to a group of proteins that accelerate degradation of
            misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed
            16431915]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.170201.1,
                                           SRR1803615.254417.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318130.13/ ENSP00000318147.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-288               DA724785.1         31-318
            289-4026            AF288393.1         1-3738
            4027-5452           BC016464.1         1260-2685
            5453-6571           BC016464.1         2687-3805
            6572-6583           AI521228.1         50-61               c
            6584-6837           AL096819.17        16309-16562         c
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.3"
     gene            1..6837
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /note="ER degradation enhancing alpha-mannosidase like
                     protein 3"
                     /db_xref="GeneID:80267"
                     /db_xref="HGNC:HGNC:16787"
                     /db_xref="MIM:610214"
     exon            1..370
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..3011
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /EC_number="3.2.1.113"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; ER degradation-enhancing -mannosidase-like
                     protein 3; ER degradation-enhancing alpha-mannosidase-like
                     3; alpha-1,2-mannosidase EDEM3; ER degradation enhancer,
                     mannosidase alpha-like 3"
                     /codon_start=1
                     /product="ER degradation-enhancing alpha-mannosidase-like
                     protein 3 isoform 2 precursor"
                     /protein_id="NP_079467.3"
                     /db_xref="CCDS:CCDS1363.2"
                     /db_xref="GeneID:80267"
                     /db_xref="HGNC:HGNC:16787"
                     /db_xref="MIM:610214"
                     /translation="MSEAGGRGCGSPVPQRARWRLVAATAAFCLVSATSVWTAGAEPM
                     SREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGK
                     FSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHS
                     LAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEART
                     GTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINI
                     HTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLD
                     VHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTD
                     FRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVR
                     TGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSIS
                     KKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEE
                     SFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAED
                     GLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRG
                     FVASSKPSNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGS
                     SSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYEEVEVLLSDKAKDRDP
                     EMENEEQPSSENDSQNQSGEQISSSSQEVDLVDQESSEENSLNSHPESLSLADMDNAA
                     SISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSIL
                     ADWNEDIEAFEMMEKDEL"
     exon            371..416
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            417..517
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            518..557
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            558..670
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            671..824
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            825..959
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1065
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1163
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1289
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1373
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1457
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1582
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1748
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1903
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..2057
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2249
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2415
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2601
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..6837
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6550..6555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /note="hexamer: AATAAA"
     polyA_site      6571
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /note="major polyA site"
     regulatory      6812..6817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
                     /note="hexamer: AATAAA"
     polyA_site      6837
                     /gene="EDEM3"
                     /gene_synonym="C1orf22; CDG2V"
ORIGIN      
        1 gctccgggat actgagggcg gagggcggtg gcagcgctgg cgctggggac cggcttggtg
       61 gcttcgggaa acagtttggc gccggcggcc gtccgtgtta ctccgcatcc cgccccgtct
      121 cggcacggct agcagccccc tggccaccag cgtccagcaa tgtgtctcac cggccgggcg
      181 tagcagctgc gcgtgcgcgg aaccgcgggg ccatgagcga agccggcggc cggggctgtg
      241 ggtccccggt tccccagcga gcgcgatgga gactagtggc ggcgacggcc gcgttctgcc
      301 tggtgtcggc cacctccgtg tggacggcgg gggccgagcc catgagtagg gaggagaaac
      361 agaagcttgg gaatcaagta ctggaaatgt ttgatcatgc ttatggtaac tatatggaac
      421 atgcttaccc tgctgatgaa ctcatgcctt taacctgtag aggtcgagtt agaggccaag
      481 agccaagtcg cggtgacgtt gatgatgcct tgggaaaatt ttctctgaca ctgattgatt
      541 ctttggacac tcttgtggtt ttaaataaaa ctaaagaatt tgaagatgca gtgagaaaag
      601 ttttaagaga tgttaattta gataacgatg tagtcgtatc agtctttgaa acaaacatca
      661 gagttcttgg gggtcttttg ggtgggcact ccctggcaat catgctgaaa gaaaaaggtg
      721 aatatatgca gtggtacaat gatgaacttc tccaaatggc aaagcagtta ggttacaaac
      781 ttttaccggc tttcaacact accagtggcc ttccttatcc aagaattaat ttaaagtttg
      841 gcatcagaaa accagaagct cggacaggaa ctgagacaga tacctgtaca gcttgtgcag
      901 gtaccttgat ccttgaattt gctgctttaa gtcgattcac aggagcaaca atatttgagg
      961 aatatgccag aaaagctctt gattttctct gggaaaaaag acagcgaagt agtaatttag
     1021 tgggcgtgac tataaatatt catactggag attgggtacg aaaagatagt ggagttggag
     1081 cagggattga ttcatattat gaatatctgt tgaaagccta tgtcttgctt ggagatgaca
     1141 gttttctgga aagatttaac acacactatg atgccataat gaggtatatt agccagccac
     1201 ctcttctact tgatgtgcat atccacaaac caatgctgaa tgctcggact tggatggatg
     1261 ctttgcttgc cttcttccca ggcttgcagg tgttaaaggg ggatattaga cctgctattg
     1321 aaactcatga aatgttatat caggtgatta aaaaacacaa ttttctacca gaggcattta
     1381 ccacagattt cagagtacac tgggctcaac atcctttaag gccagaattt gcagaaagta
     1441 cctacttctt atataaagct acaggagatc cttactacct tgaagtaggg aagacactta
     1501 ttgaaaattt aaataaatat gctagagtgc cttgcggatt tgctgccatg aaggatgttc
     1561 gtactggaag tcatgaggac agaatggatt ctttcttctt ggctgaaatg tttaaatatc
     1621 tttacctgtt atttgctgat aaagaagaca ttatttttga catagaagat tacatcttta
     1681 caacagaagc tcatctgtta cctctttggc tctctactac aaatcaaagc atctctaaaa
     1741 agaacacaac ctcggaatat acagaactgg atgacagtaa cttcgattgg acttgtccaa
     1801 atactcagat cctctttcct aatgacccat tgtatgctca aagtattcgt gagcccttga
     1861 aaaatgtggt ggataagagc tgtcctagag gcatcatcag agtagaggag agtttcagga
     1921 gtggagctaa accccctctg agagccagag atttcatggc cactaaccct gagcatttag
     1981 aaatcctgaa gaagatgggg gtgagtttga ttcacctcaa agatgggaga gtccagttgg
     2041 tccaacatgc aatccaagct gctagttcaa tcgacgctga agatgggttg aggttcatgc
     2101 aggagatgat tgaattgtca agtcagcaac aaaaagaaca gcagctgcct ccacgagctg
     2161 tacaaattgt ttcccaccca ttttttggca gggtagtatt gactgctgga ccagctcagt
     2221 ttgggctgga tctgtctaaa cataaagaga caagaggatt tgttgcaagc agtaaaccat
     2281 ccaatggttg ttcagagctt actaacccag aggcagtgat gggaaaaatc gcactgatac
     2341 aaagaggaca gtgcatgttt gcagaaaagg cacgcaacat ccagaatgct ggagccattg
     2401 gtggcattgt tattgatgac aatgagggga gcagcagtga tactgcccct ctgttccaga
     2461 tggcaggtga tggaaaggat acagatgaca tcaagatccc catgctgttc ttattcagca
     2521 aagaaggaag tatcatactg gatgccatcc gggaatatga ggaggtagaa gtgctcctct
     2581 ctgataaagc aaaagatcga gatcctgaaa tggaaaatga agaacaacca tcctctgaaa
     2641 atgattctca gaatcagagt ggtgaacaga tttcatcaag ttctcaggag gttgatttgg
     2701 ttgatcaaga gtcttctgag gaaaattctc taaattctca cccagaatca ttatctctag
     2761 cagatatgga caatgctgca agcatttccc cttctgaaca gacttctaat cccacagaaa
     2821 accatgagac tacaaatctt aatggtgaat gtacagattt agataaccag cttcaagaac
     2881 aatcagaaac tgaggaagat tccaatccta atgttagctg gggtaaaaag gtccagccta
     2941 tagactccat attagcagac tggaatgaag atatagaagc atttgaaatg atggagaagg
     3001 atgagctatg acttgctaaa caatctgttg gtaggtattt aaaaagaaaa ggtaaactgt
     3061 gtgtggttaa tagacatcct aatactaagc aggctttcta atgggaggct ttaagtatgg
     3121 tgatgaacaa ccacgttctg actggcgtag ttatcgtagg aatctggagc atgctgtgtt
     3181 agaattgacc ttgtttaaaa ctgtcccatc aaaaatggaa atccacagtt cccccttcaa
     3241 atgcagcact gcaccaccct gcaacacctc aggccaggga aagattactg agcattcctg
     3301 cgaaccagat ttctgttgtc tctggataga caagaaacaa aattcattta gtagtggagt
     3361 ggggaatagg agtttggata gccttctaat taaaggaagc tcgcctttct tggttttggg
     3421 ggttagaggc tcttttggga agatgcatcc gagtattgtg gcattctgat tatgctgcct
     3481 tcacaaaaca ctctaagtga cctaagtggt tatgaagcaa atgcatttat ggtgaaaaca
     3541 gtctttgctc attgctttct cttgtttcat ttagtgacaa atgatcaaga tgacttgatt
     3601 ttttttcctt cttaacaatg tcttttttat ttaaaccaaa ggtgaagcca gtgtactttc
     3661 tcagtgagtt ctctgcataa agactaatca gtgggaccag gtaaaaaggt catataatac
     3721 attgtggaga ttgcttactt aatacttctg aaaaatggag taagggagaa actgtaatgt
     3781 tgcaatatga acctcccatt gggccttcca tagggaaagc tgtgactact ctgaaatgga
     3841 acctagcatt atatccttgt agggtagatt ataaatcatt tccagttcat ttctcttaga
     3901 ggtgattacc tctagccatc agccttactc catcccatgt ttggtatgca atttgagcca
     3961 caaggctcgt atcgccaaca gctatataca ttttgttcca tttttctgtc ttacagagcc
     4021 atgatagaac tgtggttagt gagttaaaat tcctggagta actactgttt ttctcctttg
     4081 aaacttaggt ttctaaagtt gcacctaagg aatctgtcac attttctgtt gaatcatggt
     4141 ttttgttttt gtttttaaca gatattcctt ctgatacgga cttgaaaatt agtgtatggt
     4201 gacctgtgtt taaaaaaaaa agtacaatac aactacatat agctatatag cttaatgaga
     4261 cttccacccc cccccttttt ttttttggtt tgttgttgtt gtagtagtct ggtgctggcc
     4321 acatttaagt cttaaaaatt tttaaatttt gttgttgatg tttgtagaca gccctgttgt
     4381 tgaaatcatg gctttattca ttttatttat tttttaaact tgcctgaatt tgttctaaag
     4441 gaatatttaa gagacataat tttcttctct ttaccataac attacacaaa actttttcct
     4501 aaaacacggt tgtgaggtac tgatgaggtg taagtggagc tgttaaaaac agcagtgctg
     4561 tattgcagtt atgtatattc gtgtacagta tgtttagatc ccaggtaaac atattctttt
     4621 ctgagaggat aaatacctgc attcagatat tccaggtaaa tataattgag tcagggagta
     4681 gtaaatctga tggagaattc actttgggga ggggaaaaag aatagtatgc aagaccctta
     4741 ttggctttta attatacctg aaaccaaaat ggatattttt agtctctctg catgtgagat
     4801 ttggtgtaac aagatagaac tataatatat acagtatatg gaaggataga tatagtgctt
     4861 tgttcatttt aattgcaaag ctgccaaaat agttgaagct taattacttg acttgccttg
     4921 atttatagga ctggggcttg gagaaaatga gcagatgttc ctctaagaca tcgattacag
     4981 aagccttata tacatggatt tgattttgta tttgtagctg aaagtcactg ttgtctaaaa
     5041 ctaacttttc taagttatca aaacaaccta atttcttttc caacaaggag aacttaatgg
     5101 catgaaggat tgtgtgacac attggaaaag ccagcttact gccactctct tcctttggcc
     5161 attagaggga ggtgttgcct ttcattgacg cttagaagca aattgttcac ttgttaagaa
     5221 aagtaaatcc ttaaaaaaag aaaaggaaaa aatttaacca atttttctta atacccagaa
     5281 ggaattatac tcaatatttc cctagttaag aaaagaggag ataatgttcg tctaaaaaac
     5341 tccaacgttg taattacaac tctacattca tttacttact tgacatactg ccacaaagta
     5401 gtttttgagt tcattaaaaa ttccaaaggc attagttgtt tttttttttt ttagtgtttt
     5461 gttttttagt taacttttat agacatttta gtaacttgct aaagattcag gggattctat
     5521 gaaaccccga atttagaaac atctggtcta cctcagttaa atgttgactg cttagaaata
     5581 tagctgaagt gatcaccaca gccataaaat tgtttaagaa agatttatat aatgtttaca
     5641 aatctggaat caaggatttt agctgaaatc ctttaagaga tattagagca agtatttaat
     5701 tcaggtattt tcaagtttta aaacttaacc tgtttaccta ctaaaaataa aatagctagt
     5761 ttttttctgc atataaaagt tcattgaaat gatatgccct tatttgcaat acttttccca
     5821 taaagtttta agtgtgaaag aattgtaatt tactagatat gtttggtatg ggatattttg
     5881 ttaggcaagt tttctttttt cttcttaaat tgcaataggc ttccaaaaag agtataattg
     5941 tttcagaaca aattaactct tggcattata cgtctccctt tttctttaca gtattagtaa
     6001 aaagaaaaat tgtacacttt ctgattttaa cttcactaat gtaattactc tctcaagaag
     6061 cttttaaaat ttaaattacc atcacacaac cttttttata gtaaagccaa catttgttct
     6121 ctcaccaaac cccatgccaa attcatcatg aagaaagctc agcataagta attcaaatac
     6181 tgcttataat tttagagggg ggtagaattt agtaaatatt ccagccggtc gttttatgca
     6241 caaggcttca gtcagaacat agaaaaaaaa aacattctgt gaatgaaata ttgtatgttc
     6301 agattttata aaagacattt ttaaaagccc aatttacagc cgtatatttt cttatgatgt
     6361 aatttatgaa aaagatgtct gtactaacag gtgctgtaac actactgttg ttggatttta
     6421 ttgtttggtg ataaatgtat acaatatttc taagggaaac tatgtactgt gatgtaaaag
     6481 tctgggcaaa atgtatataa tcctgtatat aattatgtat ttgattataa ttactgattg
     6541 taaagattta ataaaatatg taaatattcc agtttagttt taatttgaat ttttagaatc
     6601 actgtgcata tcctcatact tttatgtctt gatgtttatc actctgtttt cctttttttt
     6661 ttaaactcat tttatcctcc ggggattgaa ttctggggac attttaggct gtagaaaccc
     6721 aagcttgtgg gctaaagtaa ggattttgtt ctaagaaatc actgaaagtt tttcaacaag
     6781 tcagtagcat gcacaaaggt aatgctaagt gaataaaact ctagaattgt gtataaa
//
