LOCUS       NM_022360                919 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens epididymal protein 3B (EDDM3B), mRNA.
ACCESSION   NM_022360
VERSION     NM_022360.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 919)
  AUTHORS   Premzl,M.
  TITLE     Comparative genomic analysis of eutherian ribonuclease A genes
  JOURNAL   Mol Genet Genomics 289 (2), 161-167 (2014)
   PUBMED   24337645
REFERENCE   3  (bases 1 to 919)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   4  (bases 1 to 919)
  AUTHORS   Lasserre,A., Barrozo,R., Tezon,J.G., Miranda,P.V. and
            Vazquez-Levin,M.H.
  TITLE     Human epididymal proteins and sperm function during fertilization:
            un update
  JOURNAL   Biol Res 34 (3-4), 165-178 (2001)
   PUBMED   11715855
  REMARK    Review article
REFERENCE   5  (bases 1 to 919)
  AUTHORS   Kirchhoff,C., Osterhoff,C., Pera,I. and Schroter,S.
  TITLE     Function of human epididymal proteins in sperm maturation
  JOURNAL   Andrologia 30 (4-5), 225-232 (1998)
   PUBMED   9739419
REFERENCE   6  (bases 1 to 919)
  AUTHORS   Kirchhoff,C.
  TITLE     Molecular characterization of epididymal proteins
  JOURNAL   Rev Reprod 3 (2), 86-95 (1998)
   PUBMED   9685187
  REMARK    Review article
REFERENCE   7  (bases 1 to 919)
  AUTHORS   Kirchhoff,C., Pera,I., Rust,W. and Ivell,R.
  TITLE     Major human epididymis-specific gene product, HE3, is the first
            representative of a novel gene family
  JOURNAL   Mol Reprod Dev 37 (2), 130-137 (1994)
   PUBMED   7514008
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL163636.7, AY358865.1, BX112699.1 and AI075915.1.
            
            On May 17, 2019 this sequence version replaced NM_022360.4.
            
            Summary: Testicular sperm are morphologically differentiated but
            are not progressively motile nor able to fertilize an egg.
            Post-testicular maturation requires exposure of spermatozoa to the
            microenvironment of the epididymal lumen. Spermatozoa undergo
            extensive changes in the epididymis, including enzymatic
            modifications, loss of pre-existing components and addition of new
            glycoproteins from epididymal secretions. These modifying proteins
            and enzymes are synthesized by epithelial cells lining the
            epididymal duct and secreted apically into the lumen, where they
            come into contact with, and may be absorbed onto, the sperm
            membranes. The proteins encoded by the genes in this cluster are
            synthesized and secreted by epididymal epithelial cells. [provided
            by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358865.1, SRR5189652.104446.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267784 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326783.4/ ENSP00000314810.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AL163636.7         200492-200514
            24-129              AY358865.1         1-106
            130-519             BX112699.1         103-492
            520-919             AI075915.1         2-401               c
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..919
                     /gene="EDDM3B"
                     /gene_synonym="EP3B; FAM12B; HE3-BETA; HE3B; RAM2"
                     /note="epididymal protein 3B"
                     /db_xref="GeneID:64184"
                     /db_xref="HGNC:HGNC:19223"
                     /db_xref="MIM:611582"
     exon            1..103
                     /gene="EDDM3B"
                     /gene_synonym="EP3B; FAM12B; HE3-BETA; HE3B; RAM2"
                     /inference="alignment:Splign:2.1.0"
     exon            104..919
                     /gene="EDDM3B"
                     /gene_synonym="EP3B; FAM12B; HE3-BETA; HE3B; RAM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..565
                     /gene="EDDM3B"
                     /gene_synonym="EP3B; FAM12B; HE3-BETA; HE3B; RAM2"
                     /note="family with sequence similarity 12, member B
                     (epididymal); human epididymis-specific 3 beta; epididymal
                     secretory protein E3-beta; human epididymis-specific
                     protein 3-beta; ribonuclease A M2"
                     /codon_start=1
                     /product="epididymal secretory protein E3-beta precursor"
                     /protein_id="NP_071755.1"
                     /db_xref="CCDS:CCDS9557.1"
                     /db_xref="GeneID:64184"
                     /db_xref="HGNC:HGNC:19223"
                     /db_xref="MIM:611582"
                     /translation="MASSLKIWGTLLALLCILCTLLVQSKEVSWREFMKQHYLSPSRE
                     FREYKCDVLMRENEALKDKSSHMFIYISWYKIEHICTSDNWMDRFRNAYVWVQNPLKV
                     LKCHQENSKNSYTESRSFNYIEFHCSMDGYVDSIEDLKMVEPIGN"
ORIGIN      
        1 agatttccag ggccctgagg aaaggaggac tcagactctg ctcttcagat ccacacactg
       61 atcccaacta caaccagtga cctgaacttg gagtctgagt agggcggccc cggtgactga
      121 gatggcatcg tctctaaaga tctggggcac actcttggcc ctactttgca tcctatgcac
      181 actgcttgta cagagcaaag aagtttcttg gagagaattc atgaaacagc actacttaag
      241 tccaagtcga gaattcagag agtacaaatg tgatgtcctc atgagagaaa atgaagctct
      301 gaaagacaag agctctcaca tgtttatcta tatctcatgg tacaaaatcg agcatatatg
      361 cactagtgac aactggatgg atcgcttccg aaatgcatat gtatgggtcc agaatcctct
      421 caaagtactc aagtgtcacc aggagaattc caaaaatagc tacacagaga gcaggagctt
      481 caactacatt gaattccatt gtagcatgga cgggtatgtt gatagcatag aagacctaaa
      541 gatggtagaa cctatcggca actagaaagt ctatgcacat cctcaggtat tggtagagta
      601 ttcagtgctt tctaagtagc agcccctgcc tccatcaata gtcctaccac tcccctcttg
      661 catttatttg tcaatgtttt ccaaatactt agagttatga atagcataat ttcttgatac
      721 cataactttg cctgtgttgt ttctctgcct ggaatacact tttgtcttca tttacctaat
      781 ttactcttat acttttgtcg acattcagct catatggcat ctgttcttga ttaacccaag
      841 actttcctga tattgactct cttgatacat acccaagctg aacaatcttc ccttccctaa
      901 ataaattata tgactgcaa
//
