LOCUS       NM_006683                932 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens epididymal protein 3A (EDDM3A), mRNA.
ACCESSION   NM_006683
VERSION     NM_006683.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 932)
  AUTHORS   Premzl,M.
  TITLE     Comparative genomic analysis of eutherian ribonuclease A genes
  JOURNAL   Mol Genet Genomics 289 (2), 161-167 (2014)
   PUBMED   24337645
REFERENCE   3  (bases 1 to 932)
  AUTHORS   Lasserre,A., Barrozo,R., Tezon,J.G., Miranda,P.V. and
            Vazquez-Levin,M.H.
  TITLE     Human epididymal proteins and sperm function during fertilization:
            un update
  JOURNAL   Biol Res 34 (3-4), 165-178 (2001)
   PUBMED   11715855
  REMARK    Review article
REFERENCE   4  (bases 1 to 932)
  AUTHORS   Kirchhoff,C., Osterhoff,C., Pera,I. and Schroter,S.
  TITLE     Function of human epididymal proteins in sperm maturation
  JOURNAL   Andrologia 30 (4-5), 225-232 (1998)
   PUBMED   9739419
REFERENCE   5  (bases 1 to 932)
  AUTHORS   Kirchhoff,C.
  TITLE     Molecular characterization of epididymal proteins
  JOURNAL   Rev Reprod 3 (2), 86-95 (1998)
   PUBMED   9685187
  REMARK    Review article
REFERENCE   6  (bases 1 to 932)
  AUTHORS   Kirchhoff,C., Pera,I., Rust,W. and Ivell,R.
  TITLE     Major human epididymis-specific gene product, HE3, is the first
            representative of a novel gene family
  JOURNAL   Mol Reprod Dev 37 (2), 130-137 (1994)
   PUBMED   7514008
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL163636.7, X76383.2 and BC096698.1.
            
            On May 17, 2019 this sequence version replaced NM_006683.4.
            
            Summary: Testicular sperm are morphologically differentiated but
            are not progressively motile nor able to fertilize an egg.
            Post-testicular maturation requires exposure of spermatozoa to the
            microenvironment of the epididymal lumen. Spermatozoa undergo
            extensive changes in the epididymis, including enzymatic
            modifications, loss of pre-existing components and addition of new
            glycoproteins from epididymal secretions. These modifying proteins
            and enzymes are synthesized by epithelial cells lining the
            epididymal duct and secreted apically into the lumen, where they
            come into contact with, and may be absorbed onto, the sperm
            membranes. The proteins encoded by the genes in this cluster are
            synthesized and secreted by epididymal epithelial cells. [provided
            by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.362600.1,
                                           SRR5189667.376613.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326842.3/ ENSP00000315098.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AL163636.7         177975-178027
            54-99               X76383.2           1-46
            100-652             BC096698.1         37-589
            653-932             AL163636.7         180189-180468
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..932
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /note="epididymal protein 3A"
                     /db_xref="GeneID:10876"
                     /db_xref="HGNC:HGNC:16978"
                     /db_xref="MIM:611580"
     exon            1..106
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /inference="alignment:Splign:2.1.0"
     exon            107..932
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..576
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /note="human epididymis-specific 3 alpha; family with
                     sequence similarity 12, member A; epididymal secretory
                     protein E3-alpha; human epididymis-specific protein
                     3-alpha; ribonuclease A M1; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="epididymal secretory protein E3-alpha precursor"
                     /protein_id="NP_006674.2"
                     /db_xref="CCDS:CCDS9556.1"
                     /db_xref="GeneID:10876"
                     /db_xref="HGNC:HGNC:16978"
                     /db_xref="MIM:611580"
                     /translation="MTSSLKIWGILLALLCILCRLCVYSNNIYWREFIKLHYLSPSRE
                     FKEYKCDVLMREKEALKGKSFHMFIYSLWFKIQRACINEKGSDRYRNAYVWAPGALKV
                     LECHWEKYNNRYTESRSFSYIEFHCGVDGYVDNIEDLRIIEPISN"
     regulatory      914..919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /note="hexamer: AATATA"
     polyA_site      932
                     /gene="EDDM3A"
                     /gene_synonym="EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA;
                     RAM1"
                     /note="major polyA site"
ORIGIN      
        1 agatttccag ggctctgaga aaaggaggag gacgcagact ctgctcttca gatccacatg
       61 ctgatcccca ctacaatcag tgacctgaac tcagagtcca agtaggacac gcaggtggac
      121 gtggtgactg agatgacatc ctctctaaag atttggggca tactcttggc cctgctttgc
      181 atcctttgca ggctgtgtgt atacagtaac aacatttact ggagagaatt cataaaactt
      241 cattacttaa gtccaagtcg agaattcaaa gagtacaaat gtgatgtcct catgagagaa
      301 aaagaggctc tgaaaggcaa gagctttcat atgttcatct atagcttatg gttcaaaatt
      361 cagcgtgcat gcatcaatga gaaggggagc gaccgatata gaaatgcata tgtatgggcc
      421 ccaggtgccc tcaaagtact cgagtgtcac tgggagaagt acaacaatag gtacacagag
      481 agcagaagct tcagctacat tgaattccat tgtggcgtag atggatatgt tgataacata
      541 gaagacctga ggattataga acctatcagc aactagaaag tctatgcaca tcctcagata
      601 ttggtagagt attcagtgct tccaaagtgg tgggccctgc ctccatcaat agcccctgcc
      661 actccccgct tacatttatg tgtcagtgtt ttccaactac ttagagttta tgtacctcgt
      721 gatttcttga taccaaatct ttgtgtggtt tctgtatctg taatacaatt ttgtcctaat
      781 ttgcctaatt tacacccaca ttttttccaa gattcagctc atatggcatc tgtcctcgac
      841 taacctaaga ctttcctgat attgactctc tttataccta cccaagctga acgaccctcc
      901 ttttcttaaa taaaatatat tattctaacg ta
//
