LOCUS       NM_001258315            4136 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens epithelial cell transforming 2 (ECT2), transcript
            variant 1, mRNA.
ACCESSION   NM_001258315
VERSION     NM_001258315.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Lv,E., Sheng,J., Yu,C., Rao,D. and Huang,W.
  TITLE     Long noncoding RNA MAPKAPK5-AS1 promotes metastasis through
            regulation miR-376b-5p/ECT2 axis in hepatocellular carcinoma
  JOURNAL   Dig Liver Dis 55 (7), 945-954 (2023)
   PUBMED   36567178
  REMARK    GeneRIF: Long noncoding RNA MAPKAPK5-AS1 promotes metastasis
            through regulation miR-376b-5p/ECT2 axis in hepatocellular
            carcinoma.
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wen,C., Li,Y., Huang,Y., Wang,N., He,S., Bao,M., Zhou,H. and Wu,L.
  TITLE     CircSETD3 mediates acquired resistance to gefitinib in non-small
            lung cancer cells by FXR1/ECT2 pathway
  JOURNAL   Int J Biochem Cell Biol 154, 106344 (2023)
   PUBMED   36503048
  REMARK    GeneRIF: CircSETD3 mediates acquired resistance to gefitinib in
            non-small lung cancer cells by FXR1/ECT2 pathway.
REFERENCE   3  (bases 1 to 4136)
  AUTHORS   Li,L., Liu,P., He,C. and Xu,C.
  TITLE     miRNA-223-3p regulates ECT2 to promote proliferation, invasion, and
            metastasis of gastric cancer through the Wnt/beta-catenin signaling
            pathway
  JOURNAL   J Cancer Res Clin Oncol 149 (1), 121-134 (2023)
   PUBMED   36355210
  REMARK    GeneRIF: miRNA-223-3p regulates ECT2 to promote proliferation,
            invasion, and metastasis of gastric cancer through the
            Wnt/beta-catenin signaling pathway.
REFERENCE   4  (bases 1 to 4136)
  AUTHORS   Liu,X., Zhang,J., Ju,S., Liu,L., Sun,Y., Guo,L., Zhen,Q., Han,S.,
            Lu,W. and Zhang,Y.
  TITLE     ECT2 promotes malignant phenotypes through the activation of the
            AKT/mTOR pathway and cisplatin resistance in cervical cancer
  JOURNAL   Cancer Gene Ther 30 (1), 62-73 (2023)
   PUBMED   36056253
  REMARK    GeneRIF: ECT2 promotes malignant phenotypes through the activation
            of the AKT/mTOR pathway and cisplatin resistance in cervical
            cancer.
REFERENCE   5  (bases 1 to 4136)
  AUTHORS   Husser,M.C., Ozugergin,I., Resta,T., Martin,V.J.J. and Piekny,A.J.
  TITLE     Cytokinetic diversity in mammalian cells is revealed by the
            characterization of endogenous anillin, Ect2 and RhoA
  JOURNAL   Open Biol 12 (11), 220247 (2022)
   PUBMED   36416720
  REMARK    GeneRIF: Cytokinetic diversity in mammalian cells is revealed by
            the characterization of endogenous anillin, Ect2 and RhoA.
REFERENCE   6  (bases 1 to 4136)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   7  (bases 1 to 4136)
  AUTHORS   Kimura,K., Tsuji,T., Takada,Y., Miki,T. and Narumiya,S.
  TITLE     Accumulation of GTP-bound RhoA during cytokinesis and a critical
            role of ECT2 in this accumulation
  JOURNAL   J Biol Chem 275 (23), 17233-17236 (2000)
   PUBMED   10837491
REFERENCE   8  (bases 1 to 4136)
  AUTHORS   Tatsumoto,T., Xie,X., Blumenthal,R., Okamoto,I. and Miki,T.
  TITLE     Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in
            G2/M phases, and involved in cytokinesis
  JOURNAL   J Cell Biol 147 (5), 921-928 (1999)
   PUBMED   10579713
REFERENCE   9  (bases 1 to 4136)
  AUTHORS   Takai,S., Long,J.E., Yamada,K. and Miki,T.
  TITLE     Chromosomal localization of the human ECT2 proto-oncogene to
            3q26.1-->q26.2 by somatic cell analysis and fluorescence in situ
            hybridization
  JOURNAL   Genomics 27 (1), 220-222 (1995)
   PUBMED   7665179
REFERENCE   10 (bases 1 to 4136)
  AUTHORS   Miki,T., Smith,C.L., Long,J.E., Eva,A. and Fleming,T.P.
  TITLE     Oncogene ect2 is related to regulators of small GTP-binding
            proteins
  JOURNAL   Nature 362 (6419), 462-465 (1993)
   PUBMED   8464478
  REMARK    Erratum:[Nature 1993 Aug 19;364(6439):737]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB229273.1, DQ847274.1 and
            BC070038.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001258315.1.
            
            Summary: The protein encoded by this gene is a guanine nucleotide
            exchange factor and transforming protein that is related to
            Rho-specific exchange factors and yeast cell cycle regulators. The
            expression of this gene is elevated with the onset of DNA synthesis
            and remains elevated during G2 and M phases. In situ hybridization
            analysis showed that expression is at a high level in cells
            undergoing mitosis in regenerating liver. Thus, this protein is
            expressed in a cell cycle-dependent manner during liver
            regeneration, and is thought to have an important role in the
            regulation of cytokinesis. Several transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Mar 2017].
            
            Transcript Variant: This variant (1), as well as variants 8, 9, and
            10, encodes the isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.242051.1,
                                           SRR1660807.193227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392692.8/ ENSP00000376457.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-154               DB229273.1         42-195
            155-2899            DQ847274.1         1-2745
            2900-4136           BC070038.1         2809-4045
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.31"
     gene            1..4136
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /note="epithelial cell transforming 2"
                     /db_xref="GeneID:1894"
                     /db_xref="HGNC:HGNC:3155"
                     /db_xref="MIM:600586"
     exon            1..132
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            133..284
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..2899
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /note="isoform a is encoded by transcript variant 1;
                     protein ECT2; epithelial cell-transforming sequence 2
                     oncogene"
                     /codon_start=1
                     /product="protein ECT2 isoform a"
                     /protein_id="NP_001245244.1"
                     /db_xref="CCDS:CCDS58860.1"
                     /db_xref="GeneID:1894"
                     /db_xref="HGNC:HGNC:3155"
                     /db_xref="MIM:600586"
                     /translation="MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEE
                     EMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKSDEKLIKSVINMD
                     IKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQK
                     GEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLV
                     ANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCIL
                     SFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQ
                     EWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSR
                     ETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQ
                     SARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDI
                     FDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEK
                     QKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTL
                     EKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLG
                     EHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKK
                     VLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSDELPKENWLKMLCRHVANTICKA
                     DAENLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALM
                     TSHGSVEGRSPSSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI
                     "
     exon            285..364
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            365..457
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            458..640
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            641..730
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            731..838
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            839..912
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1043
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1159
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1222
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1445
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1582
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1702
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1771
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1882
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1979
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2061
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2140
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2260
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2399
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2554
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2662
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2809
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..4136
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3823..3828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /note="hexamer: ATTAAA"
     polyA_site      3846
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
     regulatory      4112..4117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /note="hexamer: AATAAA"
     polyA_site      4136
                     /gene="ECT2"
                     /gene_synonym="ARHGEF31"
                     /note="major polyA site"
ORIGIN      
        1 aatcggcatg gctcttagag agagcagctt agtttttgaa tcggttgtgg cggccgccgg
       61 cgaggaatgg cggtatttgt gagaggagtc ggcgtttgaa gaggtggaac tcctagggct
      121 tttttgagag tgctgattta gaagaataca aatcatggct gaaaatagtg tattaacatc
      181 cactactggg aggactagct tggcagactc ttccattttt gattctaaag ttactgagat
      241 ttccaaggaa aacttactta ttggatctac ttcatatgta gaagaagaga tgcctcagat
      301 tgaaacaaga gtgatattgg ttcaagaagc tggaaaacaa gaagaactta taaaagcctt
      361 aaagactatt aaaataatgg aagtccctgt tataaagata aaagaaagtt gtcctggaaa
      421 atcggatgaa aaattaataa aaagtgttat taatatggac attaaagtgg gctttgtaaa
      481 gatggagtca gtggaagaat ttgaaggttt ggattctccg gaatttgaaa atgtatttgt
      541 agtcacggac tttcaggatt ctgtctttaa tgacctctac aaggctgatt gtagagttat
      601 tggaccacca gttgtattaa attgttcaca aaaaggagag cctttgccat tttcatgtcg
      661 cccgttgtat tgtacaagta tgatgaatct agtactatgc tttactggat ttaggaaaaa
      721 agaagaacta gtcaggttgg tgacattggt ccatcacatg ggtggagtta ttcgaaaaga
      781 ctttaattca aaagttacac atttggtggc aaattgtaca caaggagaaa aattcagggt
      841 tgctgtgagt ctaggtactc caattatgaa gccagaatgg atttataaag cttgggaaag
      901 gcggaatgaa caggatttct atgcagcagt tgatgacttt agaaatgaat ttaaagttcc
      961 tccatttcaa gattgtattt taagtttcct gggattttca gatgaagaga aaaccaatat
     1021 ggaagaaatg actgaaatgc aaggaggtaa atatttaccg cttggagatg aaagatgcac
     1081 tcaccttgta gttgaagaga atatagtaaa agatcttccc tttgaacctt caaagaaact
     1141 ttatgttgtc aagcaagagt ggttctgggg aagcattcaa atggatgccc gagctggaga
     1201 aactatgtat ttatatgaaa aggcaaatac tcctgagctc aagaaatcag tgtcaatgct
     1261 ttctctaaat acccctaaca gcaatcgcaa acgacgtcgt ttaaaagaaa cacttgctca
     1321 gctttcaaga gagacagacg tgtcaccatt tccaccccgt aagcgcccat cagctgagca
     1381 ttccctttcc atagggtcac tcctagatat ctccaacaca ccagagtcta gcattaacta
     1441 tggagacacc ccaaagtctt gtactaagtc ttctaaaagc tccactccag ttccttcaaa
     1501 gcagtcagca aggtggcaag ttgcaaaaga gctttatcaa actgaaagta attatgttaa
     1561 tatattggca acaattattc agttatttca agtaccattg gaagaggaag gacaacgtgg
     1621 tggacctatc cttgcaccag aggagattaa gactattttt ggtagcatcc cagatatctt
     1681 tgatgtacac actaagataa aggatgatct tgaagacctt atagttaatt gggatgagag
     1741 caaaagcatt ggtgacattt ttctgaaata ttcaaaagat ttggtaaaaa cctaccctcc
     1801 ctttgtaaac ttctttgaaa tgagcaagga aacaattatt aaatgtgaaa aacagaaacc
     1861 aagatttcat gcttttctca agataaacca agcaaaacca gaatgtggac ggcagagcct
     1921 tgttgaactt cttatccgac cagtacagag gttacccagt gttgcattac ttttaaatga
     1981 tcttaagaag catacagctg atgaaaatcc agacaaaagc actttagaaa aagctattgg
     2041 atcactgaag gaagtaatga cgcatattaa tgaggataag agaaaaacag aagctcaaaa
     2101 gcaaattttt gatgttgttt atgaagtaga tggatgccca gctaatcttt tatcttctca
     2161 ccgaagctta gtacagcggg ttgaaacaat ttctctaggt gagcacccct gtgacagagg
     2221 agaacaagta actctcttcc tcttcaatga ttgcctagag atagcaagaa aacggcacaa
     2281 ggttattggc acttttagga gtcctcatgg ccaaacccga cccccagctt ctcttaagca
     2341 tattcaccta atgcctcttt ctcagattaa gaaggtattg gacataagag agacagaaga
     2401 ttgccataat gcttttgcct tgcttgtgag gccaccaaca gagcaggcaa atgtgctact
     2461 cagtttccag atgacatcag atgaacttcc aaaagaaaac tggctaaaga tgctgtgtcg
     2521 acatgtagct aacaccattt gtaaagcaga tgctgagaat cttatttata ctgctgatcc
     2581 agaatccttt gaagtaaata caaaagatat ggacagtaca ttgagtagag catcaagagc
     2641 aataaaaaag acttcaaaaa aggttacaag agcattctct ttctccaaaa ctccaaaaag
     2701 agctcttcga agggctctta tgacatccca cggctcagtg gagggaagaa gtccttccag
     2761 caatgataag catgtaatga gtcgtctttc tagcacatca tcattagcag gtatcccttc
     2821 tccctccctt gtcagccttc cttccttctt tgaaaggaga agtcatacgt taagtagatc
     2881 tacaactcat ttgatatgaa gcgttaccaa aatcttaaat tatagaaatg tatagacacc
     2941 tcatactcaa ataagaaact gacttaaatg gtacttgtaa ttagcacttg gtgaaagctg
     3001 gaaggaagat aaataacact aaactatgct atttgatttt tcttcttgaa agagtaaggt
     3061 ttacctgtta cattttcaag ttaattcatg taaaaaatga tagtgatttt gatgtaattt
     3121 atctcttgtt tgaatctgtc attcaaaggc caataattta agttgctatc agctgatatt
     3181 agtagctttg caaccctgat agagtaaata aattttatgg gcgggtgcca aatactgctg
     3241 tgaatctatt tgtatagtat ccatgaatga atttatggaa atagatattt gtgcagctca
     3301 atttatgcag agattaaatg acatcataat actggatgaa aacttgcata gaattctgat
     3361 taaatagtgg gtctgtttca catgtgcagt ttgaagtatt taaataacca ctcctttcac
     3421 agtttatttt cttctcaagc gttttcaaga tctagcatgt ggattttaaa agatttgccc
     3481 tcattaacaa gaataacatt taaaggagat tgtttcaaaa tatttttgca aattgagata
     3541 aggacagaaa gattgagaaa cattgtatat tttgcaaaaa caagatgttt gtagctgttt
     3601 cagagagagt acggtatatt tatggtaatt ttatccacta gcaaatcttg atttagtttg
     3661 atagtgtgtg gaattttatt ttgaaggata agaccatggg aaaattgtgg taaagactgt
     3721 ttgtaccctt catgaaataa ttctgaagtt gccatcagtt ttactaatct tctgtgaaat
     3781 gcatagatat gcgcatgttc aactttttat tgtggtctta taattaaatg taaaattgaa
     3841 aattcatttg ctgtttcaaa gtgtgatatc tttcacaata gcctttttat agtcagtaat
     3901 tcagaataat caagttcata tggataaatg catttttatt tcctatttct ttagggagtg
     3961 ctacaaatgt ttgtcactta aatttcaagt ttctgtttta atagttaact gactatagat
     4021 tgttttctat gccatgtatg tgccacttct gagagtagta aatgactctt tgctacattt
     4081 taaaagcaat tgtattagta agaactttgt aaataaatac ctaaaaccca agtgta
//
