LOCUS       NM_032411                753 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens ECRG4 augurin precursor (ECRG4), mRNA.
ACCESSION   NM_032411
VERSION     NM_032411.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Xie,Z., Li,W., Ai,J., Xie,J. and Zhang,X.
  TITLE     C2orf40 inhibits metastasis and regulates chemo-resistance and
            radio-resistance of nasopharyngeal carcinoma cells by influencing
            cell cycle and activating the PI3K/AKT/mTOR signaling pathway
  JOURNAL   J Transl Med 20 (1), 264 (2022)
   PUBMED   35676661
  REMARK    GeneRIF: C2orf40 inhibits metastasis and regulates chemo-resistance
            and radio-resistance of nasopharyngeal carcinoma cells by
            influencing cell cycle and activating the PI3K/AKT/mTOR signaling
            pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  AUTHORS   Huo,H., Yang,S., Wu,H., Sun,Y., Zhao,R., Ye,R., Yan,D., Shi,X. and
            Yang,J.
  TITLE     Brain endothelial cells-derived extracellular vesicles
            overexpressing ECRG4 inhibit glioma proliferation through
            suppressing inflammation and angiogenesis
  JOURNAL   J Tissue Eng Regen Med 15 (12), 1162-1171 (2021)
   PUBMED   34551201
  REMARK    GeneRIF: Brain endothelial cells-derived extracellular vesicles
            overexpressing ECRG4 inhibit glioma proliferation through
            suppressing inflammation and angiogenesis.
REFERENCE   3  (bases 1 to 753)
  AUTHORS   Chen,L., Tang,H., Liu,G., Xiao,S., Liang,D., Ma,J., Yang,Y.,
            Luo,H., Zhu,Y., Xie,F., Cheng,X., Chi,J. and Wu,X.
  TITLE     MicroRNA-196b promotes gastric cancer progression by targeting
            ECRG4
  JOURNAL   Anticancer Drugs 32 (2), 127-137 (2021)
   PUBMED   33417325
  REMARK    GeneRIF: MicroRNA-196b promotes gastric cancer progression by
            targeting ECRG4.
REFERENCE   4  (bases 1 to 753)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 753)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 753)
  AUTHORS   Li,L.W., Yu,X.Y., Yang,Y., Zhang,C.P., Guo,L.P. and Lu,S.H.
  TITLE     Expression of esophageal cancer related gene 4 (ECRG4), a novel
            tumor suppressor gene, in esophageal cancer and its inhibitory
            effect on the tumor growth in vitro and in vivo
  JOURNAL   Int J Cancer 125 (7), 1505-1513 (2009)
   PUBMED   19521989
  REMARK    GeneRIF: The restoration of ECRG4 expression in ESCC cells
            inhibited cell proliferation, colony formation, cell cycle
            progression and tumor growth in vivo. ECRG4 is a novel candidate
            tumor suppressor gene in ESCC.
REFERENCE   7  (bases 1 to 753)
  AUTHORS   Mori,Y., Ishiguro,H., Kuwabara,Y., Kimura,M., Mitsui,A.,
            Kurehara,H., Mori,R., Tomoda,K., Ogawa,R., Katada,T., Harata,K. and
            Fujii,Y.
  TITLE     Expression of ECRG4 is an independent prognostic factor for poor
            survival in patients with esophageal squamous cell carcinoma
  JOURNAL   Oncol Rep 18 (4), 981-985 (2007)
   PUBMED   17786363
  REMARK    GeneRIF: Significantly lower expression of esophageal
            cancer-related gene 4 is associated with esophageal squamous cell
            carcinoma
REFERENCE   8  (bases 1 to 753)
  AUTHORS   Mirabeau,O., Perlas,E., Severini,C., Audero,E., Gascuel,O.,
            Possenti,R., Birney,E., Rosenthal,N. and Gross,C.
  TITLE     Identification of novel peptide hormones in the human proteome by
            hidden Markov model screening
  JOURNAL   Genome Res 17 (3), 320-327 (2007)
   PUBMED   17284679
REFERENCE   9  (bases 1 to 753)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 753)
  AUTHORS   Yue,C.M., Deng,D.J., Bi,M.X., Guo,L.P. and Lu,S.H.
  TITLE     Expression of ECRG4, a novel esophageal cancer-related gene,
            downregulated by CpG island hypermethylation in human esophageal
            squamous cell carcinoma
  JOURNAL   World J Gastroenterol 9 (6), 1174-1178 (2003)
   PUBMED   12800218
  REMARK    GeneRIF: Inactivation of ECRG 4 gene by hypermethylation is a
            frequent molecular event in esophageal squamous cell carcinoma and
            may be involved in the carcinogenesis of this cancer.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF325503.1 and BC021742.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032411.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021742.1, ERR279855.2782.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000238044.8/ ENSP00000238044.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-632               AF325503.1         32-663
            633-750             BC021742.1         668-785
            751-753             BC021742.1         789-791
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.2"
     gene            1..753
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /note="ECRG4 augurin precursor"
                     /db_xref="GeneID:84417"
                     /db_xref="HGNC:HGNC:24642"
                     /db_xref="MIM:611752"
     exon            1..156
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..524
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /note="esophageal cancer related gene 4 protein; augurin"
                     /codon_start=1
                     /product="augurin precursor"
                     /protein_id="NP_115787.1"
                     /db_xref="CCDS:CCDS2072.1"
                     /db_xref="GeneID:84417"
                     /db_xref="HGNC:HGNC:24642"
                     /db_xref="MIM:611752"
                     /translation="MAASPARPAVLALTGLALLLLLCWGPGGISGNKLKLMLQKREAP
                     VPTKTKVAVDENKAKEFLGSLKRQKRQLWDRTRPEVQQWYQQFLYMGFDEAKFEDDIT
                     YWLNRDRNGHEYYGDYYQRHYDEDSAIGPRSPYGFRHGASVNYDDY"
     exon            157..204
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /inference="alignment:Splign:2.1.0"
     exon            205..362
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /inference="alignment:Splign:2.1.0"
     exon            363..753
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      728..733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /note="hexamer: AATAAA"
     polyA_site      753
                     /gene="ECRG4"
                     /gene_synonym="C2orf40"
                     /note="major polyA site"
ORIGIN      
        1 acccctctcc cgcgcccggt tctccctcgc agcacctcga agtgcgcccc tcgccctcct
       61 gctcgcgccc cgccgccatg gctgcctccc ccgcgcggcc tgctgtcctg gccctgaccg
      121 ggctggcgct gctcctgctc ctgtgctggg gcccaggtgg cataagtgga aataaactca
      181 agctgatgct tcaaaaacga gaagcacctg ttccaactaa gactaaagtg gccgttgatg
      241 agaataaagc caaagaattc cttggcagcc tgaagcgcca gaagcggcag ctgtgggacc
      301 ggactcggcc cgaggtgcag cagtggtacc agcagtttct ctacatgggc tttgacgaag
      361 cgaaatttga agatgacatc acctattggc ttaacagaga tcgaaatgga catgaatact
      421 atggcgatta ctaccaacgt cactatgatg aagactctgc aattggtccc cggagcccct
      481 acggctttag gcatggagcc agcgtcaact acgatgacta ctaaccatga cttgccacac
      541 gctgtacaag aagcaaatag cgattctctt catgtatctc ctaatgcctt acactacttg
      601 gtttctgatt tgctctattt cagcagatct tttctaccta ctttgtgtga tcaaaaaaga
      661 agagttaaaa caacacatgt aaatgccttt tgatatttca tgggaatgcc tctcatttaa
      721 aaatagaaat aaagcatttt gttaaaaaga aaa
//
