LOCUS       NM_001364929            7215 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens Ecm29 proteasome adaptor and scaffold (ECPAS),
            transcript variant 3, mRNA.
ACCESSION   NM_001364929 XM_005251852
VERSION     NM_001364929.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Wang,X., Chemmama,I.E., Yu,C., Huszagh,A., Xu,Y., Viner,R.,
            Block,S.A., Cimermancic,P., Rychnovsky,S.D., Ye,Y., Sali,A. and
            Huang,L.
  TITLE     The proteasome-interacting Ecm29 protein disassembles the 26S
            proteasome in response to oxidative stress
  JOURNAL   J Biol Chem 292 (39), 16310-16320 (2017)
   PUBMED   28821611
  REMARK    GeneRIF: Data suggest that the adapter protein ECM29 is the main
            proteasome-interacting protein responsible for oxidative
            stress-induced proteasome disassembly/remodeling; ECM29 appears to
            intrude on interaction between the 20S core particle and 19S
            regulatory particle in 26S proteasome, disrupting proteasome
            structure in response to oxidative stress.
REFERENCE   3  (bases 1 to 7215)
  AUTHORS   Haratake,K., Sato,A., Tsuruta,F. and Chiba,T.
  TITLE     KIAA0368-deficiency affects disassembly of 26S proteasome under
            oxidative stress condition
  JOURNAL   J Biochem 159 (6), 609-618 (2016)
   PUBMED   26802743
  REMARK    GeneRIF: Ecm29 is involved in the dissociation process of 26S
            proteasome, providing clue to analyse the mechanism of proteasomal
            degradation under various stress condition.
REFERENCE   4  (bases 1 to 7215)
  AUTHORS   Gorbea,C., Rechsteiner,M., Vallejo,J.G. and Bowles,N.E.
  TITLE     Depletion of the 26S proteasome adaptor Ecm29 increases Toll-like
            receptor 3 signaling
  JOURNAL   Sci Signal 6 (295), ra86 (2013)
   PUBMED   24084648
  REMARK    GeneRIF: The absence of Ecm29 enhanced TLR3 signaling, which was
            characterized by the increased abundance of the adaptor protein and
            E3 ubiquitin ligase tumor necrosis factor receptor-associated
            factor 3
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7215)
  AUTHORS   Gorbea,C., Pratt,G., Ustrell,V., Bell,R., Sahasrabudhe,S.,
            Hughes,R.E. and Rechsteiner,M.
  TITLE     A protein interaction network for Ecm29 links the 26 S proteasome
            to molecular motors and endosomal components
  JOURNAL   J Biol Chem 285 (41), 31616-31633 (2010)
   PUBMED   20682791
  REMARK    GeneRIF: Ecm29 serves as an adaptor for coupling 26 S proteasomes
            to specific cellular compartments.
REFERENCE   6  (bases 1 to 7215)
  AUTHORS   Gorbea,C., Goellner,G.M., Teter,K., Holmes,R.K. and Rechsteiner,M.
  TITLE     Characterization of mammalian Ecm29, a 26 S proteasome-associated
            protein that localizes to the nucleus and membrane vesicles
  JOURNAL   J Biol Chem 279 (52), 54849-54861 (2004)
   PUBMED   15496406
  REMARK    GeneRIF: Ecm29 protein serves to couple the 26 S proteasome to
            secretory compartments engaged in quality control and to other
            sites of enhanced proteolysis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL159168.15 and AL354661.14.
            
            On Jul 17, 2018 this sequence version replaced XM_005251852.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.957.1,
                                           SRR7346977.2889682.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684092.1/ ENSP00000507419.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-268               AL159168.15        55890-56157         c
            269-372             AL159168.15        44671-44774         c
            373-503             AL159168.15        23199-23329         c
            504-620             AL159168.15        16152-16268         c
            621-739             AL159168.15        14080-14198         c
            740-889             AL159168.15        12146-12295         c
            890-1058            AL159168.15        8714-8882           c
            1059-1198           AL159168.15        5007-5146           c
            1199-1280           AL159168.15        2321-2402           c
            1281-1400           AL354661.14        137785-137904       c
            1401-1486           AL354661.14        135486-135571       c
            1487-1565           AL354661.14        135161-135239       c
            1566-1615           AL354661.14        132942-132991       c
            1616-1682           AL354661.14        131877-131943       c
            1683-1805           AL354661.14        131664-131786       c
            1806-1909           AL354661.14        129760-129863       c
            1910-2033           AL354661.14        127626-127749       c
            2034-2114           AL354661.14        126015-126095       c
            2115-2337           AL354661.14        124172-124394       c
            2338-2429           AL354661.14        123638-123729       c
            2430-2564           AL354661.14        121757-121891       c
            2565-2727           AL354661.14        120723-120885       c
            2728-2900           AL354661.14        119784-119956       c
            2901-3002           AL354661.14        118314-118415       c
            3003-3126           AL354661.14        106773-106896       c
            3127-3272           AL354661.14        103903-104048       c
            3273-3327           AL354661.14        103423-103477       c
            3328-3442           AL354661.14        102511-102625       c
            3443-3511           AL354661.14        101499-101567       c
            3512-3629           AL354661.14        99727-99844         c
            3630-3797           AL354661.14        99299-99466         c
            3798-3877           AL354661.14        96120-96199         c
            3878-3983           AL354661.14        95080-95185         c
            3984-4031           AL354661.14        94265-94312         c
            4032-4153           AL354661.14        92954-93075         c
            4154-4304           AL354661.14        88323-88473         c
            4305-4370           AL354661.14        86219-86284         c
            4371-4460           AL354661.14        84856-84945         c
            4461-4527           AL354661.14        83715-83781         c
            4528-4619           AL354661.14        83058-83149         c
            4620-4686           AL354661.14        82913-82979         c
            4687-4878           AL354661.14        82172-82363         c
            4879-5087           AL354661.14        81364-81572         c
            5088-5131           AL354661.14        80465-80508         c
            5132-5324           AL354661.14        80178-80370         c
            5325-5463           AL354661.14        78778-78916         c
            5464-5569           AL354661.14        76265-76370         c
            5570-5658           AL354661.14        75982-76070         c
            5659-5730           AL354661.14        73331-73402         c
            5731-7215           AL354661.14        70428-71912         c
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q31.3"
     gene            1..7215
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /note="Ecm29 proteasome adaptor and scaffold"
                     /db_xref="GeneID:23392"
                     /db_xref="HGNC:HGNC:29020"
                     /db_xref="MIM:616694"
     exon            1..268
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            269..372
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     CDS             351..5870
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /note="isoform 3 is encoded by transcript variant 3;
                     homolog of yeast Ecm29; ECM29 homolog, proteasome
                     accessory protein; proteasome-associated protein ECM29
                     homolog; ecm29 proteasome adapter and scaffold; proteasome
                     adapter and scaffold protein ECM29"
                     /codon_start=1
                     /product="proteasome adapter and scaffold protein ECM29
                     isoform 3"
                     /protein_id="NP_001351858.1"
                     /db_xref="CCDS:CCDS94456.1"
                     /db_xref="GeneID:23392"
                     /db_xref="HGNC:HGNC:29020"
                     /db_xref="MIM:616694"
                     /translation="MYHIDCRDQLERVFLRLGHAETDEQLQNIISKFLPPVLLKLSST
                     QEGVRKKVMELLVHLNKRIKSRPKIQLPVETLLVQYQDPAAVSFVTNFTIIYVKMGYP
                     RLPVEKQCELAPTLLTAMEGKPQPQQDSLMHLLIPTLFHMKYPVESSKSASPFNLAEK
                     PKTVQLLLDFMLDVLLMPYGYVLNESQSRQNSSSAQGSSSNSGGGSGIPQPPPGMSFY
                     AAKRVIGDNPWTPEQLEQCKLGIVKFIEAEQVPELEAVLHLVIASSDTRHSVATAADL
                     ELKSKQSLIDWNNPAIINKMYKVYLGDIPLKTKEGAVLKPELKRDPVSTRVKLKIVPH
                     LLRSRQAAETFPANIQVVYDGLFGTNTNSKLRTLSLQFVHHICITCPEIKIKPLGPML
                     LNGLTKLINEYKEDPKLLSMAYSAVGKLSSRMPHLFTKDIALVQQLFEALCKEEPETR
                     LAIQEALSMMVGAYSTLEGAQRTLMEALVASYLIKPEVQVRQVAVKFASTVFPSDHIP
                     SRYLLLLAAGDPREEVHGEAQRVLRCLPGRNRKESTSEQMPSFPEMVYYIQEKASHRM
                     KTPVKYMTGTTVLPFNPAAFGEIVLYLRMCLAHSAGVVPTSQSLADMQDHAPAIGRYI
                     RTLMSSGQMAPSSSNKSGETNPVQIYIGLLQQLLAGVGGLPVMYCLLEAVSVYPEKLA
                     TKFVDKTEWIKSLMNNSKEEMRELAALFYSVVVSTVSGNELKSMIEQLIKTTKDNHSP
                     EIQHGSLLALGFTVGRYLAKKKMRMSEQQDLERNADTLPDQEELIQSATETIGSFLDS
                     TSPLLAIAACTALGEIGRNGPLPIPSEGSGFTKLHLVESLLSRIPSSKETNKMKERAI
                     QTLGYFPVGDGDFPHQKLLLQGLMDSVEAKQIELQFTIGEAITSAAIGTSSVAARDAW
                     QMTEEEYTPPAGAKVNDVVPWVLDVILNKHIISPNPHVRQAACIWLLSLVRKLSTHKE
                     VKSHLKEIQSAFVSVLSENDELSQDVASKGLGLVYELGNEQDQQELVSTLVETLMTGK
                     RVKHEVSGETVVFQGGALGKTPDGQGLSTYKELCSLASDLSQPDLVYKFMNLANHHAM
                     WNSRKGAAFGFNVIATRAGEQLAPFLPQLVPRLYRYQFDPNLGIRQAMTSIWNALVTD
                     KSMVDKYLKEILQDLVKNLTSNMWRVRESSCLALNDLLRGRPLDDIIDKLPEIWETLF
                     RVQDDIKESVRKAAELALKTLSKVCVKMCDPAKGAAGQRTIAALLPCLLDKGMMSTVT
                     EVRALSINTLVKISKSAGAMLKPHAPKLIPALLESLSVLEPQVLNYLSLRATEQEKAA
                     MDSARLSAAKSSPMMETINMCLQYLDVSVLGELVPRLCELIRSGVGLGTKGGCASVIV
                     SLTTQCPQDLTPYSGKLMSALLSGLTDRNSVIQKSCAFAMGHLVRTSRDSSTEKLLQK
                     LNGWYMEKEEPIYKTSCALTIHAIGRYSPDVLKNHAKEVLPLAFLGMHEIADEEKSEK
                     EECNLWTEVWQENVPGSFGGIRLYLQELITITQKALQSQSWKMKAQGAIAMASIAKQT
                     SSLVPPYLGMILTALLQGLAGRTWAGKEELLKAIACVVTACSAELEKSVPNQPSTNEI
                     LQAVLKECSKENVKYKIVAISCAADILKATKEDRFQEFSNIVIPLIKKNSLESSGVRT
                     TKNEEENEKEKELQLEYLLGAFESLGKAWPRNAETQRCYRQELCKLMCERLKLSTWKV
                     QLGVLQSMNAFFQGLMLLEEEHADPEALAEILLETCKSITYSLENKTYSSVRTEALSV
                     IELLLKKLEESKQWECLTSECRVLLIESLATMEPDSRPELQEKAALLKKTLENLE"
     exon            373..503
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            504..620
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            621..739
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            740..889
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1058
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1198
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1280
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1400
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1486
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1565
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1615
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1682
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1805
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1909
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2033
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2114
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2337
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2429
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2430..2564
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2727
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2900
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            2901..3002
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3003..3126
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3272
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3273..3327
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3442
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3443..3511
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3512..3629
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3797
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3798..3877
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..3983
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            3984..4031
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4032..4153
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4154..4304
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4305..4370
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4460
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4461..4527
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4528..4619
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4620..4686
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4687..4878
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            4879..5087
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5088..5131
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5132..5324
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5325..5463
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5464..5569
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5570..5658
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5659..5730
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     exon            5731..7215
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5953
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
     regulatory      5993..5998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /note="hexamer: AATAAA"
     polyA_site      6014
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /note="major polyA site"
     regulatory      7183..7188
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /note="hexamer: AATAAA"
     polyA_site      7215
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
ORIGIN      
        1 gagtccacgt ctcttccttt ggacaggcgt gtttccccag gtcgggccca gccaacgtga
       61 cagccgcaga cctcggaaaa gggcgctccg gccccgcctc gaggatttcc cccggccgct
      121 agggcagccc gggcccgctc ggcccagccc ctcacggact gcggcgcgcg gctccccggg
      181 gcccggcccg ccgaacgcct cgctctacag ccctcacctc gcggcgccga gcgcagcgtc
      241 cgcggcatgg agaccggctc cgactcagat tctttataca attgcatatg tacgagtgga
      301 tgaacgtacc attggtgatc ccgaggattt ttgtcaagat tggataaacc atgtatcaca
      361 ttgattgtag agatcagctt gaacgggtct ttttacgact tggccatgct gaaacagatg
      421 aacaattaca gaatattata tctaaattcc ttcctcctgt tttgctcaaa ctctctagca
      481 cccaagaagg agtacgtaaa aaggtaatgg aactgctggt ccatctgaat aaacgtataa
      541 aaagccgccc caaaatacaa cttccagtag agacactgtt ggttcagtac caggaccctg
      601 ctgcagtttc ctttgtcaca aattttacta taatttatgt taaaatgggc tatcctcgcc
      661 taccagtgga aaaacaatgt gaactggccc ctacgcttct tactgccatg gaagggaagc
      721 ctcagccaca gcaggatagc ttaatgcatc ttttaatacc aacccttttt cacatgaaat
      781 accctgttga atcatcaaaa tcagcttctc catttaatct tgctgagaaa ccaaagactg
      841 tgcagctgct tttggacttc atgctagatg tccttctgat gccttatggt tacgtgttaa
      901 atgaatccca gagtcgccaa aattcatctt cagcacaggg ttcttcttca aacagtggcg
      961 gaggttctgg aatcccacag cctcctccgg gaatgagctt ttatgcagcc aaacgagtta
     1021 ttggtgataa cccatggaca cctgaacaat tggaacagtg caaattggga atcgtgaaat
     1081 tcatagaagc tgaacaggtg cctgaacttg aagctgttct ccacttggtg attgcctcta
     1141 gtgatacacg ccacagtgtg gcaacggcag cagacctgga attgaaaagc aaacagagct
     1201 taattgactg gaataatcct gccatcatta ataagatgta caaggtgtac cttggagata
     1261 taccactgaa gacaaaagag ggtgcagttc tgaagccaga gttgaaaagg gaccctgtca
     1321 gtacaagagt caagttaaag attgtccccc atctcctccg ctctagacaa gctgctgaaa
     1381 cgttcccagc caacattcag gtggtgtatg atggactttt tggtacaaat acaaattcaa
     1441 agttaagaac attatccctg caatttgtgc atcatatttg tataacctgt ccagaaatca
     1501 agattaagcc attaggtcca atgcttttga atggcctcac caagctaatc aatgaataca
     1561 aagaggaccc taaactactg tcaatggcat attcagctgt tggaaaactc tccagtcgga
     1621 tgccacattt attcactaag gatatagcgc ttgtgcagca gctttttgaa gccctttgca
     1681 aggaagagcc tgagactcga cttgctattc aagaagcttt atctatgatg gttggagcgt
     1741 atagtacttt ggaaggggca cagcgaactc tcatggaggc acttgtggct tcgtacttaa
     1801 taaagcctga agttcaagtt cgacaagtgg ctgtgaaatt tgccagtacg gtgtttccct
     1861 cagatcatat cccttccaga tatttgctgc tactggctgc aggagatcca cgtgaagaag
     1921 ttcatggaga agcacaacgc gtattaaggt gtcttccagg tagaaacaga aaagaaagta
     1981 cttctgagca gatgccttcc ttcccagaaa tggtttatta catccaagaa aaggcttctc
     2041 atcgaatgaa aactccagtc aagtacatga ccgggaccac tgtccttcca tttaacccag
     2101 cagcctttgg agagatcgtt ctgtacttgc gcatgtgcct tgcgcacagt gcgggggtgg
     2161 tgcccacctc tcagagtttg gctgatatgc aggatcatgc cccagccatt gggcgctaca
     2221 tacggacttt aatgtcaagc gggcagatgg caccctcatc atctaacaag agtggggaga
     2281 ctaaccctgt ccagatctac attggcctgc ttcagcagct gttagcaggt gttggaggtt
     2341 tgccggttat gtactgtcta ttggaagctg tgtcagtgta tccagaaaag ctggctacca
     2401 aatttgtaga caaaacagaa tggataaaga gtctgatgaa taacagtaaa gaagaaatgc
     2461 gcgaactggc agcgttgttt tattctgtag tggtatcaac agtgtcgggg aatgagttga
     2521 aatcaatgat agaacagctt ataaagacta caaaagacaa tcacagcccg gagatacagc
     2581 atggatcctt gcttgcattg ggattcacgg tgggaaggta tttggctaaa aagaaaatga
     2641 gaatgtcaga gcaacaagac ctggagagaa atgctgacac cctccctgat caagaggaac
     2701 tcattcagag tgctacagaa acaataggct catttttgga cagtacatca cccctcctgg
     2761 caattgctgc ctgcacagcc ctgggtgaaa ttggcagaaa tggtccactt ccaatcccca
     2821 gtgagggatc tggctttacc aaattgcatc ttgtagaaag cttactaagt agaatacctt
     2881 ccagtaaaga aacaaataag atgaaagaac gagcaatcca aacactggga tattttccag
     2941 ttggggatgg agattttcct caccagaaac tcctcttgca aggtctgatg gattctgtgg
     3001 aggccaagca gatagaactt cagttcacta ttggcgaagc cattaccagt gctgcaatag
     3061 gaactagttc tgtggctgcc cgagatgcct ggcaaatgac tgaagaggaa tatactccac
     3121 ctgctggagc caaagtgaat gatgtggttc catgggtgtt ggatgtgatt ttaaataaac
     3181 atatcatcag ccccaaccca cacgtgaggc aagcagcctg catctggctc ctttcccttg
     3241 tcaggaagct aagtacccac aaagaagtga agtctcatct taaagaaatt caaagtgcat
     3301 ttgtttcagt tctatcagaa aatgatgaac ttagccaaga tgttgcatca aagggccttg
     3361 ggttggttta tgaactaggc aatgaacaag atcaacagga attggtttct acacttgtgg
     3421 aaacacttat gactggcaaa agagttaaac atgaagtttc tggagagaca gtggtatttc
     3481 aagggggagc tcttggcaaa acaccagatg gtcagggcct ttctacttac aaggaacttt
     3541 gttctctggc aagtgatctt agccagccag atctggtgta taaatttatg aatttagcca
     3601 accatcatgc aatgtggaac tctaggaagg gtgctgcttt tggttttaat gtaattgcta
     3661 ccagagctgg agagcagctg gctccttttc tgcctcagct agttcctcga ctttatcgtt
     3721 accagtttga tcccaacctt ggcattcgac aggccatgac aagtatttgg aatgcgttgg
     3781 tcactgacaa atccatggtg gataaatatt tgaaagaaat tcttcaagat ttggttaaga
     3841 accttacaag caatatgtgg cgagttcgag aatccagctg tttagctttg aatgatttat
     3901 tgagaggaag acccttagat gacatcattg ataaacttcc agaaatttgg gaaacgcttt
     3961 ttagagtaca agatgatatc aaggaatctg tacgaaaagc ggcagaacta gctctgaaaa
     4021 ctctgagcaa ggtctgtgtg aaaatgtgtg accctgccaa aggagcagct ggccagagaa
     4081 ccatcgctgc ccttctgcct tgccttctgg acaaaggaat gatgagcacc gtgacggaag
     4141 ttcgagccct cagcattaac acccttgtga agatcagcaa aagtgcagga gccatgttga
     4201 aaccgcatgc accaaaactc attccagctc tgctagagtc cttaagtgta ttggagcccc
     4261 aagttctcaa ttatttgagc ctccgggcga cagagcaaga aaaggctgcg atggatagtg
     4321 ctcggcttag tgctgccaaa tcttctccaa tgatggaaac aatcaacatg tgcctgcaat
     4381 accttgatgt gtcagtgctg ggcgagctag ttcctaggtt gtgtgaactg atcagaagtg
     4441 gtgtaggtct tggaactaag ggtggctgtg ccagtgtcat tgtgtcatta actactcagt
     4501 gtcctcagga cctaacacct tactcaggta aacttatgag tgctttgctg agtggcctga
     4561 cagatcggaa cagtgtgatt cagaaatctt gtgcatttgc tatgggccat ttagttcgga
     4621 cctcacggga tagcagcact gaaaaactcc tgcagaagct caatgggtgg tatatggaga
     4681 aagaagaacc tatctacaag acctcttgtg ctttgactat tcatgctatt ggacgataca
     4741 gccctgatgt attaaagaat catgcaaaag aagtcctgcc tctggcattt ttaggcatgc
     4801 atgaaattgc tgatgaggag aaatccgaaa aagaagaatg taatttatgg accgaagtgt
     4861 ggcaggaaaa cgtacctgga tcctttggtg gcattcgatt atacctgcag gagttaatta
     4921 ctattaccca gaaggctttg cagtctcagt cctggaaaat gaaagcccag ggtgcaattg
     4981 ccatggcatc aattgcaaaa cagactagct ctctagtacc tccatatctc ggaatgatac
     5041 tgaccgcatt gctgcaaggc ctggctggaa gaacgtgggc aggaaaggag gagctattga
     5101 aagccattgc ctgtgtggtg acagcttgca gtgcagagct ggaaaagtct gtgcccaatc
     5161 aacccagcac aaatgaaatt cttcaagctg ttctgaagga atgtagcaaa gagaatgtca
     5221 aatacaagat tgtagcaatc agctgtgcag ctgatatctt gaaggccacc aaagaggaca
     5281 gattccagga gttctctaac attgtcatac ctctcatcaa gaagaactca cttgaaagca
     5341 gtggggtccg gacaaccaaa aatgaagagg agaatgaaaa ggaaaaggag ctccagctgg
     5401 aatatctgct gggtgccttt gaaagcctgg gcaaagcctg gccgcgaaac gcggagaccc
     5461 aacgttgtta tcgtcaggag ctgtgcaaac tgatgtgtga acggctaaaa ctcagcacgt
     5521 ggaaagtgca gctaggagtc ctgcaatcaa tgaatgcctt ttttcagggg ttaatgcttt
     5581 tggaagaaga acatgccgat cctgaggctt tggctgaaat tctgcttgaa acttgtaaat
     5641 caatcacata ttctttagaa aataagacct actcatctgt gagaacagaa gctttatctg
     5701 tgatagaatt gctgcttaaa aaacttgaag aatctaaaca gtgggaatgt ttgacatctg
     5761 aatgcagagt gctcctaatt gagtctttag ctactatgga gccagacagc agacctgaac
     5821 tgcaggagaa agcagcgtta ctgaagaaaa cacttgaaaa tctggaataa attagaaggg
     5881 gaagaaacaa acaagtgcca tgttcattgg gggttgaagt ggtggtgttc tttgaaaaac
     5941 caagtgggaa aaagtaaaga ttaatctgta gcatgcatca ttccttggct gaaataaaaa
     6001 gaaaaagcct taaattttgt tccttattag ctttatttta ctttatgttt ttaaagtatc
     6061 tgggctgagt gagcttaatg gaaccttgga tgtttgaggg gtggttgtaa aggaaactgg
     6121 cagaaatagt tactaaaaga gttcaagctg ttattaaagg aattaataga cagagttgca
     6181 gagcaagctg gataaaaatc ttagaactgc attcaaatta gttatttcag gaaggggccc
     6241 tataaataac ttattttaac ctccctctta cacctgaaga aataggaagc tgtgtcatct
     6301 cagaggcttt ctgaggcctg tgccaaagtc agaacccagg tgtttaatgt ctggccctgg
     6361 atactttctt ttatggccat ctgccaaatc agtgctaacc ccggcttacc caagaaactt
     6421 ccaagatgag gtacgtttag ggtggcacac agcagtcttt gtgccagaac taaactgacc
     6481 cttaccattg ggtttctgaa agccagaagc taaacattgt cttgtcatgt gtttaggtgg
     6541 ttgatttgga gaaaatgatt gtttagcatc tttacagatg gagaggacag agtcagtctc
     6601 cttcggataa ttccaatcgt cttcctttta caggtggata aagtagatta ggacaagatc
     6661 acacattcta tctgtgtctg tggctgcata ttttgcaaaa ccagaaacaa aatcgcagac
     6721 tgccgtggtt tagaccattt ataatgtcaa aactgtaatg acaaatttct gttcactttt
     6781 atgctaccag tatgacttta taggggagaa ggaaagcctt ttaattagcc acctgggaat
     6841 tactctttta aaaaatgcct tttctcaaaa gaacatgata ccactccaag taaatcattg
     6901 ccactagggg ttttcctcct tccttctgtt gcccatgccc ctgcctgaaa gtgtagggta
     6961 agaggaaaaa caagtaaaac cttagtgttg atggtaaact gagaactctg tagcttacct
     7021 tggattccag agcagaagac tggtttacat tcagtactca tccttattga cgttaacacc
     7081 aggaatctcc atgtttatta tttttcgtgg aaactccatg tttattattt ttggtggtct
     7141 ctattgacca atgtatttca aacttttttg actgcaagtc ataataaaaa taacttttta
     7201 catcacgacc cagaa
//
