LOCUS       NM_206836               1368 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens enoyl-CoA delta isomerase 2 (ECI2), transcript variant
            2, mRNA.
ACCESSION   NM_206836
VERSION     NM_206836.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Chen,L., Dai,P., Liu,L., Chen,Y., Lu,Y., Zheng,L., Wang,H., Yuan,Q.
            and Li,X.
  TITLE     The lipid-metabolism enzyme ECI2 reduces neutrophil extracellular
            traps formation for colorectal cancer suppression
  JOURNAL   Nat Commun 15 (1), 7184 (2024)
   PUBMED   39169021
  REMARK    GeneRIF: The lipid-metabolism enzyme ECI2 reduces neutrophil
            extracellular traps formation for colorectal cancer suppression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1368)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1368)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1368)
  AUTHORS   Itkonen,H.M., Brown,M., Urbanucci,A., Tredwell,G., Ho Lau,C.,
            Barfeld,S., Hart,C., Guldvik,I.J., Takhar,M., Heemers,H.V.,
            Erho,N., Bloch,K., Davicioni,E., Derua,R., Waelkens,E.,
            Mohler,J.L., Clarke,N., Swinnen,J.V., Keun,H.C., Rekvig,O.P. and
            Mills,I.G.
  TITLE     Lipid degradation promotes prostate cancer cell survival
  JOURNAL   Oncotarget 8 (24), 38264-38275 (2017)
   PUBMED   28415728
  REMARK    GeneRIF: Enoyl-CoA delta isomerase 2 (ECI2) is a novel AR-target
            that promotes prostate cancer cell survival
REFERENCE   6  (bases 1 to 1368)
  AUTHORS   Suzuki,Y., Tsunoda,T., Sese,J., Taira,H., Mizushima-Sugano,J.,
            Hata,H., Ota,T., Isogai,T., Tanaka,T., Nakamura,Y., Suyama,A.,
            Sakaki,Y., Morishita,S., Okubo,K. and Sugano,S.
  TITLE     Identification and characterization of the potential promoter
            regions of 1031 kinds of human genes
  JOURNAL   Genome Res 11 (5), 677-684 (2001)
   PUBMED   11337467
REFERENCE   7  (bases 1 to 1368)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 1368)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
REFERENCE   9  (bases 1 to 1368)
  AUTHORS   Geisbrecht,B.V., Zhang,D., Schulz,H. and Gould,S.J.
  TITLE     Characterization of PECI, a novel monofunctional Delta(3),
            Delta(2)-enoyl-CoA isomerase of mammalian peroxisomes
  JOURNAL   J Biol Chem 274 (31), 21797-21803 (1999)
   PUBMED   10419495
REFERENCE   10 (bases 1 to 1368)
  AUTHORS   Suk,K., Kim,Y.H., Hwang,D.Y., Ihm,S.H., Yoo,H.J. and Lee,M.S.
  TITLE     Molecular cloning and expression of a novel human cDNA related to
            the diazepam binding inhibitor
  JOURNAL   Biochim Biophys Acta 1454 (1), 126-131 (1999)
   PUBMED   10354522
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AU100345.1 and BC002668.2.
            
            On May 20, 2020 this sequence version replaced NM_206836.2.
            
            Summary: This gene encodes a member of the hydratase/isomerase
            superfamily. The protein encoded is a key mitochondrial enzyme
            involved in beta-oxidation of unsaturated fatty acids. It catalyzes
            the transformation of 3-cis and 3-trans-enoyl-CoA esters arising
            during the stepwise degradation of cis-, mono-, and polyunsaturated
            fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively
            spliced transcript variants have been described. [provided by
            RefSeq, Aug 2011].
            
            Transcript Variant: This variant (2) uses an alternate upstream
            translational start codon and includes an alternate splice site in
            its 5' coding region, compared to variant 1. The encoded isoform
            (2) has a longer and distinct N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002668.2, AK222617.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154665, SAMEA2159080
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380118.8/ ENSP00000369461.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AU100345.1         23-23
            2-1368              BC002668.2         1-1367
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p25.2"
     gene            1..1368
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /note="enoyl-CoA delta isomerase 2"
                     /db_xref="GeneID:10455"
                     /db_xref="HGNC:HGNC:14601"
                     /db_xref="MIM:608024"
     exon            1..65
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..1200
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /EC_number="5.3.3.8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     acyl-Coenzyme A binding domain containing 2; peroxisomal
                     D3,D2-enoyl-CoA isomerase; peroxisomal 3,2-trans-enoyl-CoA
                     isomerase; hepatocellular carcinoma-associated antigen 88;
                     enoyl-CoA delta isomerase 2, mitochondrial; DBI-related
                     protein 1; dodecenoyl-CoA isomerase; testicular secretory
                     protein Li 33; renal carcinoma antigen NY-REN-1;
                     delta(3),delta(2)-enoyl-CoA isomerase; diazepam-binding
                     inhibitor-related protein 1"
                     /codon_start=1
                     /product="enoyl-CoA delta isomerase 2 isoform 2"
                     /protein_id="NP_996667.2"
                     /db_xref="CCDS:CCDS43420.2"
                     /db_xref="GeneID:10455"
                     /db_xref="HGNC:HGNC:14601"
                     /db_xref="MIM:608024"
                     /translation="MAMAYLAWRLARRSCPSSLQVTSFPVVQLHMNRTAMRASQKDFE
                     NSMNQVKLLKKDPGNEVKLKLYALYKQATEGPCNMPKPGVFDLINKAKWDAWNALGSL
                     PKEAARQNYVDLVSSLSPSLESSSQVEPGTDRKSTGFETLVVTSEDGITKIMFNRPKK
                     KNAINTEMYHEIMRALKAASKDDSIITVLTGNGDYYSSGNDLTNFTDIPPGGVEEKAK
                     NNAVLLREFVGCFIDFPKPLIAVVNGPAVGISVTLLGLFDAVYASDRATFHTPFSHLG
                     QSPEGCSSYTFPKIMSPAKATEMLIFGKKLTAGEACAQGLVTEVFPDSTFQKEVWTRL
                     KAFAKLPPNALRISKEVIRKREREKLHAVNAEECNVLQGRWLSDECTNAVVNFLSRKS
                     KL"
     exon            66..228
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            229..327
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            328..516
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            517..586
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            587..689
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            690..810
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            811..900
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1044
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1368
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1347..1352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /note="hexamer: AATAAA"
     polyA_site      1368
                     /gene="ECI2"
                     /gene_synonym="ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88;
                     PECI"
                     /note="major polyA site"
ORIGIN      
        1 agagccgccc aagggatggc gatggcgtac ttggcttgga gactggcgcg gcgttcgtgt
       61 ccgagttctc tgcaggtcac tagtttcccg gtagttcagc tgcacatgaa tagaacagca
      121 atgagagcca gtcagaagga ctttgaaaat tcaatgaatc aagtgaaact cttgaaaaag
      181 gatccaggaa acgaagtgaa gctaaaactc tacgcgctat ataagcaggc cactgaagga
      241 ccttgtaaca tgcccaaacc aggtgtattt gacttgatca acaaggccaa atgggacgca
      301 tggaatgccc ttggcagcct gcccaaggaa gctgccaggc agaactatgt ggatttggtg
      361 tccagtttga gtccttcatt ggaatcctct agtcaggtgg agcctggaac agacaggaaa
      421 tcaactgggt ttgaaactct ggtggtgacc tccgaagatg gcatcacaaa gatcatgttc
      481 aaccggccca aaaagaaaaa tgccataaac actgagatgt atcatgaaat tatgcgtgca
      541 cttaaagctg ccagcaagga tgactcaatc atcactgttt taacaggaaa tggtgactat
      601 tacagtagtg ggaatgatct gactaacttc actgatattc cccctggtgg agtagaggag
      661 aaagctaaaa ataatgccgt tttactgagg gaatttgtgg gctgttttat agattttcct
      721 aagcctctga ttgcagtggt caatggtcca gctgtgggca tctccgtcac cctccttggg
      781 ctattcgatg ccgtgtatgc atctgacagg gcaacatttc atacaccatt tagtcaccta
      841 ggccaaagtc cggaaggatg ctcctcttac acttttccga agataatgag cccagccaag
      901 gcaacagaga tgcttatttt tggaaagaag ttaacagcgg gagaggcatg tgctcaagga
      961 cttgttactg aagttttccc tgatagcact tttcagaaag aagtctggac caggctgaag
     1021 gcatttgcaa agcttccccc aaatgccttg agaatttcaa aagaggtaat caggaaaaga
     1081 gagagagaaa aactacacgc tgttaatgct gaagaatgca atgtccttca gggaagatgg
     1141 ctatcagatg aatgcacaaa tgctgtggtg aacttcttat ccagaaaatc aaaactgtga
     1201 tgaccactac agcagagtaa agcatgtcca aggaaggatg tgctgttacc tctgatttcc
     1261 agtactggaa ctaaataagc ttcattgtgc cttttgtagt gctagaatat caattacaat
     1321 gatgatattt cactacagct ctgatgaata aaaagttttg taaaacaa
//
