LOCUS       NM_001919               1507 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens enoyl-CoA delta isomerase 1 (ECI1), transcript variant
            1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001919
VERSION     NM_001919.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Bramhecha,Y.M., Guerard,K.P., Audet-Walsh,E., Rouzbeh,S.,
            Kassem,O., Pernet,E., Scarlata,E., Hamel,L., Brimo,F.,
            Divangahi,M., Aprikian,A.G., Chevalier,S., Giguere,V. and
            Lapointe,J.
  TITLE     Fatty acid oxidation enzyme Delta3, Delta2-enoyl-CoA isomerase 1
            (ECI1) drives aggressive tumor phenotype and predicts poor clinical
            outcome in prostate cancer patients
  JOURNAL   Oncogene 41 (20), 2798-2810 (2022)
   PUBMED   35411033
  REMARK    GeneRIF: Fatty acid oxidation enzyme Delta3, Delta2-enoyl-CoA
            isomerase 1 (ECI1) drives aggressive tumor phenotype and predicts
            poor clinical outcome in prostate cancer patients.
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1507)
  AUTHORS   Rasmussen,A.L., Diamond,D.L., McDermott,J.E., Gao,X., Metz,T.O.,
            Matzke,M.M., Carter,V.S., Belisle,S.E., Korth,M.J., Waters,K.M.,
            Smith,R.D. and Katze,M.G.
  TITLE     Systems virology identifies a mitochondrial fatty acid oxidation
            enzyme, dodecenoyl coenzyme A delta isomerase, required for
            hepatitis C virus replication and likely pathogenesis
  JOURNAL   J Virol 85 (22), 11646-11654 (2011)
   PUBMED   21917952
  REMARK    GeneRIF: Hepatitis C virus growth and RNA replication in hepatoma
            cell lines stably expressing coenzyme A delta isomerase
            (DCI)-targeting short hairpin RNA (shRNA) were abrogated,
            indicating that DCI is required for productive infection.
REFERENCE   4  (bases 1 to 1507)
  AUTHORS   Zhao,X., Leon,I.R., Bak,S., Mogensen,M., Wrzesinski,K., Hojlund,K.
            and Jensen,O.N.
  TITLE     Phosphoproteome analysis of functional mitochondria isolated from
            resting human muscle reveals extensive phosphorylation of inner
            membrane protein complexes and enzymes
  JOURNAL   Mol Cell Proteomics 10 (1), M110.000299 (2011)
   PUBMED   20833797
REFERENCE   5  (bases 1 to 1507)
  AUTHORS   Partanen,S.T., Novikov,D.K., Popov,A.N., Mursula,A.M.,
            Hiltunen,J.K. and Wierenga,R.K.
  TITLE     The 1.3 A crystal structure of human mitochondrial
            Delta3-Delta2-enoyl-CoA isomerase shows a novel mode of binding for
            the fatty acyl group
  JOURNAL   J Mol Biol 342 (4), 1197-1208 (2004)
   PUBMED   15351645
  REMARK    GeneRIF: determination of structure and mode of binding for fatty
            acyl group
REFERENCE   6  (bases 1 to 1507)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Behal,A., Touchman,J.W.,
            Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human lens for the NEIBank
            Project: over 2000 non-redundant transcripts, novel genes and
            splice variants
  JOURNAL   Mol Vis 8, 171-184 (2002)
   PUBMED   12107413
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1507)
  AUTHORS   Takahashi,Y., Hirata,Y., Burstein,Y. and Listowsky,I.
  TITLE     Delta 3, delta 2-enoyl-CoA isomerase is the protein that copurifies
            with human glutathione S-transferases from S-hexylglutathione
            affinity matrices
  JOURNAL   Biochem J 304 (Pt 3) (Pt 3), 849-852 (1994)
   PUBMED   7818490
REFERENCE   8  (bases 1 to 1507)
  AUTHORS   Janssen,U., Fink,T., Lichter,P. and Stoffel,W.
  TITLE     Human mitochondrial 3,2-trans-enoyl-CoA isomerase (DCI): gene
            structure and localization to chromosome 16p13.3
  JOURNAL   Genomics 23 (1), 223-228 (1994)
   PUBMED   7829074
REFERENCE   9  (bases 1 to 1507)
  AUTHORS   Kilponen,J.M., Hayrinen,H.M., Rehn,M. and Hiltunen,J.K.
  TITLE     cDNA cloning and amino acid sequence of human mitochondrial delta 3
            delta 2-enoyl-CoA isomerase: comparison of the human enzyme with
            its rat counterpart, mitochondrial short-chain isomerase
  JOURNAL   Biochem J 300 (Pt 1) (Pt 1), 1-5 (1994)
   PUBMED   8198519
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291127.1, BQ773923.1 and
            AC009065.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001919.3.
            
            Summary: This gene encodes a member of the hydratase/isomerase
            superfamily. The protein encoded is a key mitochondrial enzyme
            involved in beta-oxidation of unsaturated fatty acids. It catalyzes
            the transformation of 3-cis and 3-trans-enoyl-CoA esters arising
            during the stepwise degradation of cis-, mono-, and polyunsaturated
            fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively
            spliced transcript variants have been described. [provided by
            RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.616007.1, AK291127.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2152568 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000301729.9/
                                                  ENSP00000301729.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1010              AK291127.1         15-1024
            1011-1037           BQ773923.1         19-45               c
            1038-1507           AC009065.8         37541-38010
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1507
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /note="enoyl-CoA delta isomerase 1"
                     /db_xref="GeneID:1632"
                     /db_xref="HGNC:HGNC:2703"
                     /db_xref="MIM:600305"
     exon            1..73
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..930
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /EC_number="5.3.3.8"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; dodecenoyl-Coenzyme A delta isomerase (3,2
                     trans-enoyl-Coenzyme A isomerase); dodecenoyl-CoA delta
                     isomerase (3,2 trans-enoyl-CoA isomerase); D3,D2-enoyl-CoA
                     isomerase; acetylene-allene isomerase; 3,2-trans-enoyl-CoA
                     isomerase, mitochondrial; enoyl-CoA delta isomerase 1,
                     mitochondrial; delta(3),Delta(2)-enoyl-CoA isomerase;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="enoyl-CoA delta isomerase 1, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_001910.2"
                     /db_xref="CCDS:CCDS10464.1"
                     /db_xref="GeneID:1632"
                     /db_xref="HGNC:HGNC:2703"
                     /db_xref="MIM:600305"
                     /translation="MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGS
                     QRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPG
                     VFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALT
                     CDYRILADNPRYCIGLNETQLGIIAPFWLKDTLENTIGHRAAERALQLGLLFPPAEAL
                     QVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQN
                     FVSFISKDSIQKSLQMYLERLKEEKG"
     exon            74..187
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     exon            188..315
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     exon            316..462
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     exon            463..584
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     exon            585..763
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     exon            764..1507
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      991..996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /note="hexamer: AACAAG"
     polyA_site      1014
                     /gene="ECI1"
                     /gene_synonym="DCI"
                     /note="major polyA site"
     polyA_site      1507
                     /gene="ECI1"
                     /gene_synonym="DCI"
ORIGIN      
        1 atcccgcgcg ttgcggtcaa gatggcgctg gtggcttctg tgcgagtccc ggcgcgcgtt
       61 ctgctccgcg cgggggcccg gctcccgggc gcggccctcg ggcggacgga gcgggcggcc
      121 ggcggcggag acggcgcgcg gcgcttcggg agccagcggg tgctggtgga gccggacgcg
      181 ggcgcagggg tcgctgtgat gaaattcaag aaccccccag tgaacagcct gagcctggag
      241 tttctgacgg agctggtcat cagcctggag aagctggaga atgacaagag cttccgcggt
      301 gtcattctga cctcggaccg cccgggtgtc ttctcggccg gcctggacct gacggagatg
      361 tgtgggagga gccccgccca ctacgctggg tactggaagg ccgttcagga gctgtggctg
      421 cggttgtacc agtccaacct ggtgctggtc tccgccatca acggagcctg ccccgctgga
      481 ggctgcctgg tggccctgac ctgtgactac cgcatcctgg cggacaaccc caggtactgc
      541 ataggactca atgagaccca gctgggcatc atcgcccctt tctggttgaa agacaccctg
      601 gagaacacca tcgggcaccg ggcggcggag cgtgccctgc agctggggct gctcttcccg
      661 ccggcggagg ccctgcaggt gggcatagtg gaccaggtgg tcccggagga gcaggtgcag
      721 agcactgcgc tgtcagcgat agcccagtgg atggccattc cagaccatgc tcgacagctg
      781 accaaggcca tgatgcgaaa ggccacggcc agccgcctgg tcacgcagcg cgatgcggac
      841 gtgcagaact tcgtcagctt catctccaaa gactccatcc agaagtccct gcagatgtac
      901 ttagagaggc tcaaagaaga aaaaggctaa cgattgggct gccacaggct tacggccaca
      961 cgtgcccctg tgggtcccag ggaggtctta aacaaggtat ttttcaactt aaaagtactg
     1021 ccagcgtttc attttgctga tgttacgtag aagttcctgt tcctcatagc ccagtggccc
     1081 aaggcccagc cacacacaaa cacacatggg tgcctgtgcc cgtcaggcaa ggggagttgg
     1141 agggcagcct tttgacttgg gattgtgact ggcaggggat gccattctgg acctggtgag
     1201 gttgccacgg agtgtctctg ttcccaaggc agaatcgtaa gtcaagtccc tggtcatcca
     1261 ttctggcctt tggactgcct gctcttaggt gtaaggccag gtggtcttgg cccccatgac
     1321 ccacacagaa ctccccaggg aaaagccctt tggtcactgc ccttactcat caggatgaag
     1381 agcgcacagt gaatcagagc aactggggga tcctgagcca gcgggagtgg gactctggag
     1441 gcagctgtgc tgccagggat ccaaatgtcc cttaagaagg attttgcaag aaccaaactc
     1501 atccaaa
//
