LOCUS       NM_004215               2383 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens estrogen receptor binding site associated antigen 9
            (EBAG9), transcript variant 1, mRNA.
ACCESSION   NM_004215
VERSION     NM_004215.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Wirges,A., Bunse,M., Joedicke,J.J., Blanc,E., Gudipati,V.,
            Moles,M.W., Shiku,H., Beule,D., Huppa,J.B., Hopken,U.E. and Rehm,A.
  TITLE     EBAG9 silencing exerts an immune checkpoint function without
            aggravating adverse effects
  JOURNAL   Mol Ther 30 (11), 3358-3378 (2022)
   PUBMED   35821635
  REMARK    GeneRIF: EBAG9 silencing exerts an immune checkpoint function
            without aggravating adverse effects.
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Suwiwat,S., Tungsinmunlong,K. and Siriaungkul,S.
  TITLE     Expression of CD44v6 and RCAS1 in Uterine Cervical Carcinoma
            Infected with Human Papillomavirus and Its Effect on Cell
            Proliferation and Differentiation
  JOURNAL   Asian Pac J Cancer Prev 23 (7), 2431-2439 (2022)
   PUBMED   35901351
  REMARK    GeneRIF: Expression of CD44v6 and RCAS1 in Uterine Cervical
            Carcinoma Infected with Human Papillomavirus and Its Effect on Cell
            Proliferation and Differentiation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2383)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2383)
  AUTHORS   Szubert,S., Koper,K., Dutsch-Wicherek,M.M. and Kojs,Z.
  TITLE     The potential predictive value of serum srCaS1 levels for overall
            survival in endometrial cancer
  JOURNAL   Ginekol Pol 90 (3), 134-140 (2019)
   PUBMED   30950002
  REMARK    GeneRIF: Studied soluble cancer-associated surface antigen (srCaS1)
            levels as a biomarker for survival in endometrial cancer. Found
            elevated serum sRCAS1 to be associated with shortened overall
            survival in endometrial cancer patients.
REFERENCE   5  (bases 1 to 2383)
  AUTHORS   Dong,J., Sun,G. and Zhu,H.
  TITLE     Diagnostic value of soluble receptor-binding cancer antigen
            expressed on SiSo cells and carcinoembryonic antigen in
            differentiating malignant from benign pleural effusion
  JOURNAL   Tumour Biol 37 (3), 3257-3264 (2016)
   PUBMED   26438059
  REMARK    GeneRIF: Data suggest that joint detection of receptor-binding
            cancer antigen expressed on SiSo cells (RCAS1) and carcinoembryonic
            antigen (CEA) can improve the diagnostic sensitivity and
            specificity.
REFERENCE   6  (bases 1 to 2383)
  AUTHORS   Matsushima,T., Nakashima,M., Oshima,K., Abe,Y., Nishimura,J.,
            Nawata,H., Watanabe,T. and Muta,K.
  TITLE     Receptor binding cancer antigen expressed on SiSo cells, a novel
            regulator of apoptosis of erythroid progenitor cells
  JOURNAL   Blood 98 (2), 313-321 (2001)
   PUBMED   11435298
REFERENCE   7  (bases 1 to 2383)
  AUTHORS   Ohshima,K., Nakashima,M., Sonoda,K., Kikuchi,M. and Watanabe,T.
  TITLE     Expression of RCAS1 and FasL in human trophoblasts and uterine
            glands during pregnancy: the possible role in immune privilege
  JOURNAL   Clin Exp Immunol 123 (3), 481-486 (2001)
   PUBMED   11298137
REFERENCE   8  (bases 1 to 2383)
  AUTHORS   Ikeda,K., Sato,M., Tsutsumi,O., Tsuchiya,F., Tsuneizumi,M., Emi,M.,
            Imoto,I., Inazawa,J., Muramatsu,M. and Inoue,S.
  TITLE     Promoter analysis and chromosomal mapping of human EBAG9 gene
  JOURNAL   Biochem Biophys Res Commun 273 (2), 654-660 (2000)
   PUBMED   10873660
REFERENCE   9  (bases 1 to 2383)
  AUTHORS   Nakashima,M., Sonoda,K. and Watanabe,T.
  TITLE     Inhibition of cell growth and induction of apoptotic cell death by
            the human tumor-associated antigen RCAS1
  JOURNAL   Nat Med 5 (8), 938-942 (1999)
   PUBMED   10426319
REFERENCE   10 (bases 1 to 2383)
  AUTHORS   Watanabe,T., Inoue,S., Hiroi,H., Orimo,A., Kawashima,H. and
            Muramatsu,M.
  TITLE     Isolation of estrogen-responsive genes with a CpG island library
  JOURNAL   Mol Cell Biol 18 (1), 442-449 (1998)
   PUBMED   9418891
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD108096.1, BC022506.1,
            AA812215.1 and AI619447.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004215.4.
            
            Summary: This gene was identified as an estrogen-responsive gene.
            Regulation of transcription by estrogen is mediated by estrogen
            receptor, which binds to the estrogen-responsive element found in
            the 5'-flanking region of this gene. The encoded protein is a
            tumor-associated antigen that is expressed at high frequency in a
            variety of cancers. Alternate splicing results in multiple
            transcript variants. A pseudogene of this gene has been defined on
            chromosome 10. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022506.1, SRR1660809.202546.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337573.10/ ENSP00000337675.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-176               CD108096.1         43-218
            177-1138            BC022506.1         173-1134
            1139-1550           AA812215.1         9-420               c
            1551-1922           AI619447.1         1-372               c
            1923-2383           BC022506.1         1920-2380
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q23.2"
     gene            1..2383
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="estrogen receptor binding site associated antigen
                     9"
                     /db_xref="GeneID:9166"
                     /db_xref="HGNC:HGNC:3123"
                     /db_xref="MIM:605772"
     exon            1..289
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     exon            290..387
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..946
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="cancer-associated surface antigen RCAS1; estrogen
                     receptor-binding fragment-associated gene 9 protein;
                     receptor-binding cancer antigen expressed on SiSo cells 1"
                     /codon_start=1
                     /product="receptor-binding cancer antigen expressed on
                     SiSo cells"
                     /protein_id="NP_004206.1"
                     /db_xref="CCDS:CCDS6313.1"
                     /db_xref="GeneID:9166"
                     /db_xref="HGNC:HGNC:3123"
                     /db_xref="MIM:605772"
                     /translation="MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPT
                     TVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTI
                     RKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWE
                     EEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS"
     exon            388..466
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     exon            467..625
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     exon            626..733
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     exon            734..825
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     exon            826..2383
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1333..1338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="hexamer: AATAAA"
     polyA_site      1352
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="major polyA site"
     regulatory      1530..1535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="hexamer: AATAAT"
     polyA_site      1550
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="major polyA site"
     regulatory      1654..1659
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="hexamer: ATTATA"
     polyA_site      1677
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
     regulatory      1904..1909
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="hexamer: CATAAA"
     polyA_site      1923
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
     regulatory      2360..2365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
                     /note="hexamer: AATAAA"
     polyA_site      2383
                     /gene="EBAG9"
                     /gene_synonym="EB9; PDAF"
ORIGIN      
        1 aggcgtgcag cattcgccat gctccgctca cgcgtgggag actgggctgt ggggtaccgg
       61 cccggaaagc acgcagcctc caaagccgcc ttcctcaggg aaatttgcgt gaccttactg
      121 ccctccgtct acaggccttg tacctctcca ggccgatttt tccacaattt aaatctcagt
      181 tcacctggta tccagctcca gcaacttaga gcgtttcacg tcacgccggg cgccaggcgt
      241 cggcttgtat aacctgaaaa cgctcctgtt tttctcatct gtgcagtggg ttttgattcc
      301 caccatggcc atcacccagt ttcggttatt taaattttgt acctgcctag caacagtatt
      361 ctcattccta aagagattaa tatgcagatc tggcagagga cggaaattaa gtggagacca
      421 aataactttg ccaactacag ttgattattc atcagttcct aagcagacag atgttgaaga
      481 gtggacttcc tgggatgaag atgcacccac cagtgtaaag atcgaaggag ggaatgggaa
      541 tgtggcaaca caacaaaatt ctttggaaca actggaacct gactatttta aggacatgac
      601 accaactatt aggaaaactc agaaaattgt tattaagaag agagaaccat tgaattttgg
      661 catcccagat gggagcacag gtttctctag tagattagca gctacacaag atctgccttt
      721 tattcatcag tcttctgaat taggtgactt agatacctgg caggaaaata ccaatgcatg
      781 ggaagaagaa gaagatgcag cctggcaagc agaagaagtt ctgagacagc agaaactagc
      841 agacagagaa aagagagcag ccgaacaaca aaggaagaaa atggaaaagg aagcacaacg
      901 gctaatgaag aaggaacaaa acaaaattgg tgtgaaactt tcataacaca tgttcaaatt
      961 ttatcatgcc agtaggagaa atctcagctc cacaacccaa gcaacatttg tatggattta
     1021 agagtatttt aagaagacat actgcttgat tttaatacat tgatcaggcc atccaggaca
     1081 ccacgattct cccaaagtac cttgaactct tagtgattga gactcaaaaa aacaaaaaag
     1141 acttgagaca atgttttctt caacatgctc caaatataag acatttgttt gctgtacaga
     1201 aagtatcaca aatggaatat atcagtacct ctcaagctag tgtttctagc taaataaatg
     1261 ggtgtatata attttatggt ggaaaagaac tgtactgtct gttatgattt ccttcaatgt
     1321 gcataatgat aaaataaata attttaatat tcttttgttt ccatggttac ctgacctaaa
     1381 ttagataaat tgtagggctt tagctttctt atttttgtca aaagttggtg ttgacataca
     1441 ttccctctaa tttgaactgg tattgtttac gtttgataca acattaagga atttgatgat
     1501 tttcatttca tgaaaatgac attaaatgca ataattttac ttatcataaa cattttgtac
     1561 atcattattt tcttttggat tagtgttgtc atacatgtaa tttatatcac atgtataaac
     1621 attgaaaatc aactaaaatg acatttgttc tacattataa cttgtgagtt tcaagaacta
     1681 gtattagtag ttttttcctt tcattataga ttgtaagatg ttgtggtatc tttgagtgcc
     1741 ttagttcttc cttcctccca aaagccatta atttacaaat gcttaaagcc atcaggtcaa
     1801 atatttcaaa gcctttagat gatttctgta ctagctttag atgtctgacg ttatgtaggt
     1861 tacctgtgtt cttggctagg aaaacattat tgattcatta aatcataaag gtgggaataa
     1921 taatctttta tttatgatgt tgattggctc caaaatagtc ttaaggaaat aaatactggg
     1981 tctgtagggg aaaagtagac ttcatagttt aaaatcccat taaccttttc accgcagttg
     2041 aaatgcatcc agcctgattt tcctatcatt ttggaatttt taaggatttt tactttctta
     2101 agttactgcc tagaatcaac attcggtgag attttgaaat gtcatagata ctgtacaggc
     2161 caaaccttac taatttattt tacttaaagt gatattttat agaaaaatca taagttatac
     2221 aatgagaacc ctttaagccc ttagcctaag ctttcagact aaatgtgatt atagaataag
     2281 atgaaagtta actttggtac agagcttttt atagccccaa attatatttc cagttattat
     2341 attcattaga atttctgcta ataaactccc aacttaaata gaa
//
