LOCUS       NM_018453               1303 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens E2F associated phosphoprotein (EAPP), transcript
            variant 1, mRNA.
ACCESSION   NM_018453
VERSION     NM_018453.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1303)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 1303)
  AUTHORS   Ostensson,M., Monten,C., Bacelis,J., Gudjonsdottir,A.H.,
            Adamovic,S., Ek,J., Ascher,H., Pollak,E., Arnell,H., Browaldh,L.,
            Agardh,D., Wahlstrom,J., Nilsson,S. and Torinsson-Naluai,A.
  TITLE     A possible mechanism behind autoimmune disorders discovered by
            genome-wide linkage and association analysis in celiac disease
  JOURNAL   PLoS One 8 (8), e70174 (2013)
   PUBMED   23936387
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1303)
  AUTHORS   Andorfer,P. and Rotheneder,H.
  TITLE     Regulation of the MDR1 promoter by E2F1 and EAPP
  JOURNAL   FEBS Lett 587 (10), 1504-1509 (2013)
   PUBMED   23542036
  REMARK    GeneRIF: Independently of EAPP, E2F1 also increases the activity of
            the MDR1 promoter.
REFERENCE   6  (bases 1 to 1303)
  AUTHORS   Andorfer,P. and Rotheneder,H.
  TITLE     EAPP: gatekeeper at the crossroad of apoptosis and p21-mediated
            cell-cycle arrest
  JOURNAL   Oncogene 30 (23), 2679-2690 (2011)
   PUBMED   21258403
  REMARK    GeneRIF: results indicate that the level of EAPP is critical for
            cellular homeostasis
REFERENCE   7  (bases 1 to 1303)
  AUTHORS   Schwarzmayr,L., Andorfer,P., Novy,M. and Rotheneder,H.
  TITLE     Regulation of the E2F-associated phosphoprotein promoter by GC-box
            binding proteins
  JOURNAL   Int J Biochem Cell Biol 40 (12), 2845-2853 (2008)
   PUBMED   18588995
  REMARK    GeneRIF: Regulation of the EAPP promoter by GC-box binding proteins
            is reported.
REFERENCE   8  (bases 1 to 1303)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   9  (bases 1 to 1303)
  AUTHORS   Kamnasaran,D., Chen,C.P., Devriendt,K., Mehta,L. and Cox,D.W.
  TITLE     Defining a holoprosencephaly locus on human chromosome 14q13 and
            characterization of potential candidate genes
  JOURNAL   Genomics 85 (5), 608-621 (2005)
   PUBMED   15820313
REFERENCE   10 (bases 1 to 1303)
  AUTHORS   Novy,M., Pohn,R., Andorfer,P., Novy-Weiland,T., Galos,B.,
            Schwarzmayr,L. and Rotheneder,H.
  TITLE     EAPP, a novel E2F binding protein that modulates E2F-dependent
            transcription
  JOURNAL   Mol Biol Cell 16 (5), 2181-2190 (2005)
   PUBMED   15716352
  REMARK    GeneRIF: EAPP modulates E2F-regulated transcription, stimulates
            proliferation, and may be involved in the malignant transformation
            of cells.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC001245.1 and AL445363.7.
            
            On Nov 23, 2018 this sequence version replaced NM_018453.3.
            
            Summary: This gene encodes a phosphoprotein that interacts with
            several members of the E2F family of proteins. The protein
            localizes to the nucleus, and is present throughout the cell cycle
            except during mitosis. It functions to modulate E2F-regulated
            transcription and stimulate proliferation. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jan
            2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.178107.1, BC001245.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000250454.8/ ENSP00000250454.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-574               BC001245.1         10-583
            575-575             AL445363.7         96622-96622         c
            576-788             BC001245.1         585-797
            789-1189            AL445363.7         87898-88298         c
            1190-1303           BC001245.1         1199-1312
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q13.1"
     gene            1..1303
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="E2F associated phosphoprotein"
                     /db_xref="GeneID:55837"
                     /db_xref="HGNC:HGNC:19312"
                     /db_xref="MIM:609486"
     exon            1..147
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..931
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="E2F-associated phosphoprotein isoform 1"
                     /protein_id="NP_060923.2"
                     /db_xref="CCDS:CCDS41941.1"
                     /db_xref="GeneID:55837"
                     /db_xref="HGNC:HGNC:19312"
                     /db_xref="MIM:609486"
                     /translation="MNRLPDDYDPYAVEEPSDEEPALSSSEDEVDVLLHGTPDQKRKL
                     IRECLTGESESSSEDEFEKEMEAELNSTMKTMEDKLSSLGTGSSSGNGKVATAPTRYY
                     DDIYFDSDSEDEDRAVQVTKKKKKKQHKIPTNDELLYDPEKDNRDQAWVDAQRRGYHG
                     LGPQRSRQQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSINKEEVL
                     RYKASENRKKRRVHKKMRSNREDAAEKAETDVEEIYHPVMCTECSTEVAVYDKDEVFH
                     FFNVLASHS"
     exon            148..329
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     exon            330..425
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     exon            426..543
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     exon            544..654
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     exon            655..1303
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1205..1210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="hexamer: ATTAAA"
     polyA_site      1231
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="major polyA site"
     regulatory      1279..1284
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="hexamer: ATTAAA"
     polyA_site      1303
                     /gene="EAPP"
                     /gene_synonym="BM036; C14orf11"
                     /note="major polyA site"
ORIGIN      
        1 agggactgga cttgcagtgt aaacagagac gctgcaaatt gcttgtggac ggtgtaggcc
       61 gctgcaggcc accatgaacc ggcttccgga tgactacgac ccctacgcgg ttgaagagcc
      121 tagcgacgag gagccggctt tgagcagctc tgaggatgaa gtggatgtgc ttttacatgg
      181 aactcctgac caaaaacgaa aactcatcag agaatgtctt accggagaaa gtgaatcatc
      241 tagtgaagat gaatttgaaa aggagatgga agctgaatta aattctacca tgaaaacaat
      301 ggaggacaag ttatcctctc tgggaactgg atcttcctca ggaaatggaa aagttgcaac
      361 agctccgaca aggtactacg atgatatata ttttgattct gattccgagg atgaagacag
      421 agcagtacag gtgaccaaga aaaaaaagaa gaaacaacac aagattccaa caaatgacga
      481 attactgtat gatcctgaaa aagataacag agatcaggcc tgggttgatg cacagagaag
      541 gggttaccat ggtttgggac cacagagatc acgtcaacaa cagcctgttc caaatagtga
      601 tgctgtcttg aattgtcctg cctgcatgac cacactttgc cttgattgcc aaaggcatga
      661 atcatacaaa actcaatata gagcaatgtt tgtaatgaat tgttctatta acaaagagga
      721 ggttctaaga tataaagcct cagagaacag gaagaaaagg cgggtccata agaagatgag
      781 gtctaaccgg gaagatgctg ccgagaaggc agagacagat gtggaagaaa tctatcaccc
      841 agtcatgtgc actgaatgtt ccactgaagt ggcagtctac gacaaggatg aagtctttca
      901 ttttttcaat gttttagcaa gccattccta aacagcccaa ctggcattta attacccaat
      961 actgtatata aggcaaatat ggacagttac tttcctcttg cctgttcata tccttcagtg
     1021 acattgagga agcagtgttt ctctttttaa aggagaatag ttgtcaacct tcattcatct
     1081 cttacatctc tcaccctctc cttttttttt tctttgattt tcccccttat tgatgggact
     1141 gatattcatt ctgtttttga tgaacatttg gaaactgtcg ggctttttat taaagctctg
     1201 tagaattaaa atgttctgga attataagca atctttgttt ctgagtgtta tttttatttt
     1261 ggatatagct ttgatgtaat taaactggaa actctttcct gaa
//
