LOCUS       NM_033083               4447 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens ELL associated factor 1 (EAF1), mRNA.
ACCESSION   NM_033083
VERSION     NM_033083.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Pal,S., Yadav,D. and Biswas,D.
  TITLE     ATM-mediated ELL phosphorylation enhances its self-association
            through increased EAF1 interaction and inhibits global
            transcription during genotoxic stress
  JOURNAL   Nucleic Acids Res 50 (19), 10995-11012 (2022)
   PUBMED   36305813
  REMARK    GeneRIF: ATM-mediated ELL phosphorylation enhances its
            self-association through increased EAF1 interaction and inhibits
            global transcription during genotoxic stress.
            Erratum:[Nucleic Acids Res. 2023 Jul 7;51(12):6507. doi:
            10.1093/nar/gkad454.. PMID: 37194690]
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Chen,Y., Vos,S.M., Dienemann,C., Ninov,M., Urlaub,H. and Cramer,P.
  TITLE     Allosteric transcription stimulation by RNA polymerase II super
            elongation complex
  JOURNAL   Mol Cell 81 (16), 3386-3399 (2021)
   PUBMED   34265249
  REMARK    GeneRIF: Allosteric transcription stimulation by RNA polymerase II
            super elongation complex.
REFERENCE   3  (bases 1 to 4447)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4447)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4447)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4447)
  AUTHORS   Xiao,W., Jiang,F. and Wang,Z.
  TITLE     ELL binding regulates U19/Eaf2 intracellular localization,
            stability, and transactivation
  JOURNAL   Prostate 66 (1), 1-12 (2006)
   PUBMED   16114057
  REMARK    GeneRIF: ELL may be an important factor required for U19/Eaf2
            function because U19/Eaf2 nuclear localization and transactivation
            activity are essential for its function as a transcription factor.
REFERENCE   7  (bases 1 to 4447)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   8  (bases 1 to 4447)
  AUTHORS   Kong,S.E., Banks,C.A., Shilatifard,A., Conaway,J.W. and
            Conaway,R.C.
  TITLE     ELL-associated factors 1 and 2 are positive regulators of RNA
            polymerase II elongation factor ELL
  JOURNAL   Proc Natl Acad Sci U S A 102 (29), 10094-10098 (2005)
   PUBMED   16006523
  REMARK    GeneRIF: ELL-associated factors 1 and 2 are positive regulators of
            RNA polymerase II elongation factor ELL.
REFERENCE   9  (bases 1 to 4447)
  AUTHORS   Polak,P.E., Simone,F., Kaberlein,J.J., Luo,R.T. and Thirman,M.J.
  TITLE     ELL and EAF1 are Cajal body components that are disrupted in
            MLL-ELL leukemia
  JOURNAL   Mol Biol Cell 14 (4), 1517-1528 (2003)
   PUBMED   12686606
  REMARK    Erratum:[Mol Biol Cell.2003 May;14(5):following table of contents]
REFERENCE   10 (bases 1 to 4447)
  AUTHORS   Simone,F., Polak,P.E., Kaberlein,J.J., Luo,R.T., Levitan,D.A. and
            Thirman,M.J.
  TITLE     EAF1, a novel ELL-associated factor that is delocalized by
            expression of the MLL-ELL fusion protein
  JOURNAL   Blood 98 (1), 201-209 (2001)
   PUBMED   11418481
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL698790.1, AF272973.1, BC041329.2, AC027125.5 and BC035167.2.
            
            On Nov 22, 2018 this sequence version replaced NM_033083.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.138232.1,
                                           SRR1660805.75989.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396842.7/ ENSP00000380054.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                AL698790.1         42-69
            29-673              AF272973.1         1-645
            674-935             BC041329.2         657-918
            936-1017            AF272973.1         908-989
            1018-2392           BC041329.2         1001-2375
            2393-3754           AC027125.5         51112-52473         c
            3755-4447           BC035167.2         1357-2049
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..4447
                     /gene="EAF1"
                     /note="ELL associated factor 1"
                     /db_xref="GeneID:85403"
                     /db_xref="HGNC:HGNC:20907"
                     /db_xref="MIM:608315"
     exon            1..285
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..989
                     /gene="EAF1"
                     /note="ELL (eleven nineteen lysine-rich leukemia
                     gene)-associated factor 1"
                     /codon_start=1
                     /product="ELL-associated factor 1"
                     /protein_id="NP_149074.3"
                     /db_xref="CCDS:CCDS2626.1"
                     /db_xref="GeneID:85403"
                     /db_xref="HGNC:HGNC:20907"
                     /db_xref="MIM:608315"
                     /translation="MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRYDFKPASIDT
                     SCEGELQVGKGDEVTITLPHIPGSTPPMTVFKGNKRPYQKDCVLIINHDTGEYVLEKL
                     SSSIQVKKTRAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPL
                     KDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGP
                     ASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD"
     exon            286..380
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            381..517
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..708
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            709..942
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..4447
                     /gene="EAF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4423..4428
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EAF1"
                     /note="hexamer: AATAAA"
     polyA_site      4447
                     /gene="EAF1"
                     /note="major polyA site"
ORIGIN      
        1 ctctctcacc cccacgcaga ggagagaact tgcttctgga cccgggtggg tgccggctcg
       61 gctctccttg tcttccagag cggtggcccg gaagcacagt cctcccagac gccagcgcca
      121 gaagctcgga tcgcggctgc accgggagag cgccgatctg ggtgcgaggc aggtgcgggg
      181 ccatgaatgg gaccgcaaac ccgctgctgg accgcgagga acattgcctg aggctcgggg
      241 agagcttcga gaagcggccg cgggcctcct tccacactat tcgttatgat tttaaaccag
      301 catctataga cacttcctgt gaaggagagc ttcaagttgg caaaggagat gaagtcacaa
      361 ttacactgcc acatatccct ggatccacac cacccatgac tgtgttcaag gggaacaaac
      421 ggccttacca gaaagactgt gtgcttatta ttaatcatga cactggtgaa tatgtgctgg
      481 aaaaactcag tagcagcatt caggtgaaga aaacaagagc tgagggcagc agtaaaatcc
      541 aggcccgaat ggaacagcag cccactcgtc ctccacagac gtcacagcca ccaccacctc
      601 caccacctat gccattcaga gctccaacga agcctccagt tggacccaaa acttctccct
      661 tgaaagataa cccctcacct gaacctcagt tggatgacat caaaagagag ctgagggctg
      721 aagttgacat tattgaacaa atgagcagca gcagtgggag cagctcttca gactctgaga
      781 gctcttcggg aagtgatgac gatagctcca gcagtggagg cgaggacaat ggcccagcct
      841 ctcctccgca gccttcacac cagcagccct acaacagtag gcctgccgtt gccaatggaa
      901 ccagccggcc acaaggaagc aaccagctca tgaacaccct cagaaatgac ttgcagttga
      961 gtgagtctgg cagtgacagt gatgactagt gctggatctt tcgaaaccta ctttttggtg
     1021 cacaaacatg ccgcaagact gagctacttt ggcgtggagt ccattgcaag aggaaaatgt
     1081 tatggatcag tgactgtagt aggagtttga ggctctggaa ctctcacata ttcaagtctt
     1141 taacttagtg gtgatgggtg attttctcgt gtcatttttg aacaatctca tctttcaaag
     1201 tttaagtaga cctgtagaag agctaacaga attatatttc tatttagtta aaattgttaa
     1261 tgaaaaacag ataaatgtgc cctggtctaa gttactcaaa ggccatatct tcaccaggcc
     1321 agagattcag ttttaaacct cagcagccat cagtttggcc aagtggttgg taccagtgga
     1381 atgaagtctt tgatgggtag gagagagagg gaaaacctca aactacatca tagaagtatg
     1441 ttaaaaccac tttaaattgt tgacacaaag tggtaaggaa gttttgtctg actcagcctg
     1501 acaagcctgg atagaaatgg acctcagaat acagaatgga gtccttgagt gtcagttggt
     1561 gaggaccatg gagtcctgcc agctcaacct gtcatttaca gactaagaaa gggaagtaca
     1621 cacctggctt gtggccagtc tcctgccccc tctctgggct ttcctcactg ttctctccct
     1681 tgaatctcat ttgtaggaat caagagattc ctatacatgg gacttttaga tgtacattgg
     1741 cacttggatt agattccaaa agacaaaagt gttgggtgct tctagcttga attcagtccc
     1801 taaatacatg aggctgggtg agcagatgaa accggtagcc actgtgctga tgcatgtgac
     1861 tccattcagt tggggatttt ggttccatgt ggatccatac taagtattct tcagtcagtg
     1921 gattttagca aggggaggta ggagtggaag agaggtgtga agaaacctct ccgaacaaat
     1981 gaaccagcag taagtttcta aaaatcagaa tctagataga agttctgcaa tatgaaatga
     2041 gcacattctt tgataaggcg tatctattca tgcttttgta ccactgtttt gtacctgact
     2101 ccctgaccga tttgtatttt ttatatacaa ctagaaggaa gtcacaagat tgccttctac
     2161 agtgtgccat ttccaaatgg atctgttgtt ggaggaaact ggttgctagt caatgttcta
     2221 tatttaatga atgtgtgata aatcatcctg taatcagtat ggagtaacct gtttttgtag
     2281 tttggatgaa tatgtcctga gaaatttcca tccactttgg ttcagcggac atcaaggtag
     2341 taataataat ttttcctcca caggtccctc cactcatatg gcctctccct ccccagctag
     2401 tggaggggaa gcagtctgga cttagaaagg aaataggtgg tctgtcatag gggctttcat
     2461 tagagttaaa cttcatagag tcaactgttt catcatcata gtgagcccag agagccactg
     2521 cccagcagca tgctcacacc acctacccta gtgtaggtaa taggtctacg ctaggacccc
     2581 gtgctgggct ctcagcccat catgagattt tggtggattt aatggcaggt aggaacttat
     2641 ttatagtgga ttgataattg ctttataatt ccttggtaat gacagctcag ggaaggtttc
     2701 acaaggtcat gatcaggaga cttgaattgt tactggatgt aggaattgtt tcactgctct
     2761 taacttgctc aaactggggc aggttccagg aacttgaact aaaaatatct atttaagcct
     2821 ctctctcttt ctctcctccc caactttttt ctgaaagcct tgatttctgt agacagaact
     2881 atggtttttg gcatgttggg tcaagacgtg tttctatagg aaatgcttgc acgtggcact
     2941 ggcttggtga gtcaagcttc ctgagcttca gaccgtttgt cctgtcatct gtcctgccca
     3001 acactgtatg gtttttctct agcacggctt caattttgtt ctctgcacaa agcctttggt
     3061 ggttctgccc tctgccctcc agctttaccc accagagtaa gcaatctttt ctttttagaa
     3121 atcaagaatt tggcttcaca gatggcttct cacagatgcc ctcctgccag ccaaaccgga
     3181 cttgttcttc ctgcctctgt gccttctctc attctccttc tctccctcca taccccactt
     3241 ttgaggttct gtgggtacca tctttgtgga ctcttgtctt ttttttcaac ccacatggag
     3301 tgccctgtgt gtacatactg accttttttt tttttttttt tttttgcttg aactctggtc
     3361 ggtatgagct ccttaaaagc aggtactggg ctggctgtgg tgactcatgc ctgtaatccc
     3421 agcactttgg gaggctgagg tgggaggatt gcttgagtcc aggagtttga gaccagcctg
     3481 ggcaacatgg cgagaccctg tttctacaaa aagaaaaagg gaaaaaaaaa gcagggactg
     3541 tcattctcat ctcctccctg gggtatccac aggaatggac attcaaatgg ttgtagcctg
     3601 gtggggtaga aggcagagaa gactacccag ccagtgaagt gcagaggata gacataggaa
     3661 aacattccag gcatttggag acagctgaaa gtatccatgc tgactttcat gatttagaag
     3721 tctttaactg aattattaaa attgcctttt ttttgtgctg tacaatgtct actgtactgt
     3781 gggtcattta gatgagatga aaaacttaat taaatctgaa gtgtcagagg cgcttagtaa
     3841 gccttagtaa agttgtgatt gaagaaactt aatacaaatg aactagaggt ttgtaatcat
     3901 gccactcact aacgatttta tttctgtagc agaatcattt tttctatgta aggtgttaat
     3961 gtgtgtgtat gtgctttggt tgtaggaaaa cttgaaaatt ccaaaatcct tattttccta
     4021 tttgagaggc tggttcagca gggtgtgtgt gtgtgtgtgt gtgtgtgtgt atgaatggta
     4081 tatttattac attattttga aagagaatta gtgtgttatg tggataatgt tatatacagc
     4141 caaagtggat gtttctattt ggcaaggaag gtaggatttc tgaaactcag gccttaacca
     4201 ataggttgga agacaagacc aattgaagag ttaggaaatg tgagtttttg ttacttctgt
     4261 tattccagtc ttggtttcat tgtctcattc tttattttta aaatcttgtg cctaaaagtt
     4321 tttttgctta attatgaagt agacatgcat gtttacattt atgtaaaata tttgctgtgt
     4381 aaagtatttt ttgtttattc tcttaaaaga tcactatatt taaataaaag tgaaggtcag
     4441 caacaca
//
