LOCUS       NM_024680               3706 bp    mRNA    linear   PRI 24-JUN-2024
DEFINITION  Homo sapiens E2F transcription factor 8 (E2F8), transcript variant
            1, mRNA.
ACCESSION   NM_024680
VERSION     NM_024680.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Miki,R., Matsuo,S., Ushida,T., Tano,S., Imai,K., Nawa,A.,
            Kajiyama,H. and Kotani,T.
  TITLE     TJP1 suppresses trophoblast cell invasion by expressing E2F8 in the
            human placenta
  JOURNAL   Mol Cell Endocrinol 591, 112277 (2024)
   PUBMED   38795825
  REMARK    GeneRIF: TJP1 suppresses trophoblast cell invasion by expressing
            E2F8 in the human placenta.
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Liu,Z., Wang,C., Tang,Y., Zhang,X., Pei,J., Liu,H., Yu,Y. and Gu,W.
  TITLE     ENO1 promotes trophoblast invasion regulated by E2F8 in recurrent
            miscarriage
  JOURNAL   FASEB J 38 (8), e23631 (2024)
   PUBMED   38661062
  REMARK    GeneRIF: ENO1 promotes trophoblast invasion regulated by E2F8 in
            recurrent miscarriage.
REFERENCE   3  (bases 1 to 3706)
  AUTHORS   Lee,D.Y., Lee,S., Kim,Y.S., Park,S., Bae,S.M., Cho,E.A., Park,E.J.,
            Park,H.H., Kim,S.Y., So,I., Chun,J.N. and Jeon,J.H.
  TITLE     Cyclosporin A inhibits prostate cancer growth through suppression
            of E2F8 transcription factor in a MELK-dependent manner
  JOURNAL   Oncol Rep 50 (6) (2023)
   PUBMED   37888771
  REMARK    GeneRIF: Cyclosporin A inhibits prostate cancer growth through
            suppression of E2F8 transcription factor in a MELK-dependent
            manner.
REFERENCE   4  (bases 1 to 3706)
  AUTHORS   Liu,K., Wang,L., Lou,Z., Guo,L., Xu,Y., Qi,H., Fang,Z., Mei,L.,
            Chen,X., Zhang,X., Shao,J. and Xiang,X.
  TITLE     E2F8 exerts cancer-promoting effects by transcriptionally
            activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition
            in suppressing lung adenocarcinoma
  JOURNAL   Biochem Pharmacol 218, 115854 (2023)
   PUBMED   37863324
  REMARK    GeneRIF: E2F8 exerts cancer-promoting effects by transcriptionally
            activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition
            in suppressing lung adenocarcinoma.
REFERENCE   5  (bases 1 to 3706)
  AUTHORS   Zhang,M., Xu,Y., Zhang,Y. and Lou,G.
  TITLE     E2F8 knockdown suppresses cell proliferation and induces cell cycle
            arrest via Wnt/beta-Catenin pathway in ovarian cancer
  JOURNAL   Chin J Physiol 66 (4), 266-275 (2023)
   PUBMED   37635486
  REMARK    GeneRIF: E2F8 knockdown suppresses cell proliferation and induces
            cell cycle arrest via Wnt/beta-Catenin pathway in ovarian cancer.
REFERENCE   6  (bases 1 to 3706)
  AUTHORS   Lammens,T., Li,J., Leone,G. and De Veylder,L.
  TITLE     Atypical E2Fs: new players in the E2F transcription factor family
  JOURNAL   Trends Cell Biol 19 (3), 111-118 (2009)
   PUBMED   19201609
  REMARK    Review article
REFERENCE   7  (bases 1 to 3706)
  AUTHORS   Zalmas,L.P., Zhao,X., Graham,A.L., Fisher,R., Reilly,C.,
            Coutts,A.S. and La Thangue,N.B.
  TITLE     DNA-damage response control of E2F7 and E2F8
  JOURNAL   EMBO Rep 9 (3), 252-259 (2008)
   PUBMED   18202719
  REMARK    GeneRIF: E2F7 and E2F8 act upstream of E2F1, and influence the
            ability of cells to undergo a DNA-damage response.
REFERENCE   8  (bases 1 to 3706)
  AUTHORS   Christensen,J., Cloos,P., Toftegaard,U., Klinkenberg,D.,
            Bracken,A.P., Trinh,E., Heeran,M., Di Stefano,L. and Helin,K.
  TITLE     Characterization of E2F8, a novel E2F-like cell-cycle regulated
            repressor of E2F-activated transcription
  JOURNAL   Nucleic Acids Res 33 (17), 5458-5470 (2005)
   PUBMED   16179649
  REMARK    GeneRIF: E2F8 may have an important role in repressing the
            expression of E2F-target genes in the S-phase of the cell cycle.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3706)
  AUTHORS   Logan,N., Graham,A., Zhao,X., Fisher,R., Maiti,B., Leone,G. and La
            Thangue,N.B.
  TITLE     E2F-8: an E2F family member with a similar organization of
            DNA-binding domains to E2F-7
  JOURNAL   Oncogene 24 (31), 5000-5004 (2005)
   PUBMED   15897886
  REMARK    GeneRIF: The similarities between E2F-7 and E2F-8 define a new
            subgroup of the E2F family, and further imply that E2F-7 and E2F-8
            may act through overlapping mechanisms in mediating cell cycle
            control.
REFERENCE   10 (bases 1 to 3706)
  AUTHORS   Maiti,B., Li,J., de Bruin,A., Gordon,F., Timmers,C., Opavsky,R.,
            Patil,K., Tuttle,J., Cleghorn,W. and Leone,G.
  TITLE     Cloning and characterization of mouse E2F8, a novel mammalian E2F
            family member capable of blocking cellular proliferation
  JOURNAL   J Biol Chem 280 (18), 18211-18220 (2005)
   PUBMED   15722552
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB959621.1, BX504614.1, BU164108.1, AK055206.1, AK026964.1,
            BC028244.1 and BM972658.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024680.3.
            
            Summary: This gene encodes a member of a family of transcription
            factors which regulate the expression of genes required for
            progression through the cell cycle. The encoded protein regulates
            progression from G1 to S phase by ensuring the nucleus divides at
            the proper time. Multiple alternatively spliced variants, encoding
            the same protein, have been identified. [provided by RefSeq, Jan
            2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC126402.1, SRR7346977.2059164.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000250024.9/ ENSP00000250024.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-522               CB959621.1         67-588
            523-801             BX504614.1         284-562
            802-1273            BU164108.1         167-638
            1274-1435           AK055206.1         1231-1392
            1436-1885           AK026964.1         21-470
            1886-3341           BC028244.1         302-1757
            3342-3702           AK026964.1         1929-2289
            3703-3706           BM972658.1         16-19               c
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.1"
     gene            1..3706
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /note="E2F transcription factor 8"
                     /db_xref="GeneID:79733"
                     /db_xref="HGNC:HGNC:24727"
                     /db_xref="MIM:612047"
     exon            1..398
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            399..522
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             508..3111
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /note="E2F family member 8"
                     /codon_start=1
                     /product="transcription factor E2F8"
                     /protein_id="NP_078956.2"
                     /db_xref="CCDS:CCDS7849.1"
                     /db_xref="GeneID:79733"
                     /db_xref="HGNC:HGNC:24727"
                     /db_xref="MIM:612047"
                     /translation="MENEKENLFCEPHKRGLMKTPLKESTTANIVLAEIQPDFGPLTT
                     PTKPKEGSQGEPWTPTANLKMLISAVSPEIRNRDQKRGLFDNRSGLPEAKDCIHEHLS
                     GDEFEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNNDICLDEVAEELNVERRRIYDI
                     VNVLESLHMVSRLAKNRYTWHGRHNLNKTLGTLKSIGEENKYAEQIMMIKKKEYEQEF
                     DFIKSYSIEDHIIKSNTGPNGHPDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVML
                     FLVSTPQIVSLEVAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVT
                     EERGRKPAFKWTGPEISPNTSGSSPVIHFTPSDLEVRRSSKENCAKNLFSTRGKPNFT
                     RHPSLIKLVKSIESDRRKINSAPSSPIKTNKAESSQNSAPFPSKMAQLAAICKMQLEE
                     QSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLIPSPLSSAV
                     PLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTDATTEKA
                     ANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLK
                     GLENVSATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTS
                     SELTAVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQH
                     LGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQ
                     SVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLGTLFVPQ
                     RKLEVSTEDVH"
     exon            523..801
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            802..958
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1273
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1435
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1573
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1777
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1865
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..2400
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2533
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2928
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3706
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3685..3690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /note="hexamer: ATTAAA"
     polyA_site      3706
                     /gene="E2F8"
                     /gene_synonym="E2F-8"
                     /note="major polyA site"
ORIGIN      
        1 actgagcgag tggcggccgg gggacagtac ctgcttgcct tattgggcaa atacagcctt
       61 ccaaggtgga gtctaacgcc gtggtgtgaa ctggccaccc gaacagggag taaatagtgc
      121 tttgtatctt taaggaagcc cttttttaaa tttaaaaaaa aagtcttaat tataagactc
      181 tttaaatgcc caactgcata cttggagctt tgggactgaa tttggaactt tcctgtcaag
      241 cgacctccca cgactttact gctgagcctg tgcacgtgtg tgtaagggga gaaatccagg
      301 catctagatg cagacttgta cccagttact tggggtcgcg tgcgctcagc tgggacctgg
      361 gctcgtgcgc ttagtccgga gccctgatct gcgaacagga tattaaaact tttagtacaa
      421 ttgattggac tacttgaacc atcgggattt ggggaggaac tccagatttt tcatttttaa
      481 actctaaatg tatgaggaat ttacagaatg gagaacgaaa aggaaaatct cttttgtgag
      541 ccacataaaa ggggactaat gaaaacacct ctgaaagaat ccaccacagc aaatatcgtg
      601 ttggcagaga tccagcctga ctttggccct ttaaccacac ctaccaagcc caaggaaggc
      661 tctcagggag agccgtggac accgacagcc aacctgaaaa tgctcatcag tgctgtgagc
      721 cctgagatcc gcaacagaga tcagaaaagg ggtttgtttg acaacagaag tggattacct
      781 gaggccaaag actgtataca cgaacactta tctggagatg aatttgagaa atcccaacca
      841 agtcgaaaag agaaaagttt aggattattg tgtcataagt tcttagcacg atatcctaat
      901 tatcccaacc ctgctgtgaa taatgacatc tgccttgacg aagtggcaga ggaacttaat
      961 gttgaacgtc gacgcattta cgatatcgtg aacgtcctag agagtttaca tatggtgagc
     1021 cgcctcgcca aaaacaggta cacttggcac gggcgacaca atctcaacaa aacccttggc
     1081 accttgaaga gcatcgggga ggagaataag tacgccgagc agattatgat gatcaaaaag
     1141 aaagaatatg agcaagagtt tgactttatt aagagttaca gtatagagga tcatatcatc
     1201 aaatcaaaca ctggcccaaa tggacaccca gacatgtgtt ttgtggaact ccctggagtg
     1261 gaatttcggg cagcttctgt aaacagccgc aaagacaagt ctttaagggt aatgagccag
     1321 aaatttgtga tgctgttttt ggtgtcaacg cctcagatag taagcctaga agttgctgcc
     1381 aagattttaa ttggggagga ccatgtggaa gatttggata aaagcaagtt taaaacaaaa
     1441 attaggaggt tgtatgatat agctaatgtt ctgagtagcc tggatcttat caagaaagtt
     1501 catgttacag aggaaagagg ccgaaaacca gctttcaaat ggaccggccc agaaatcagt
     1561 ccaaatacca gtggctccag cccagtcatt cattttactc cctctgattt ggaggtgaga
     1621 cggtcttcaa aagagaactg tgccaaaaac ctcttttcca cacgtgggaa accaaacttt
     1681 actcgacacc catctcttat caaattggta aagagtatag aaagtgatcg gagaaagata
     1741 aattctgcgc ccagtagccc tatcaagacc aacaaagctg agagttctca gaattctgca
     1801 cccttcccaa gtaaaatggc tcagctcgca gctatttgta aaatgcagtt agaagagcaa
     1861 tcaagtgaat ccagacagaa agtgaaagta cagctggcaa gatctggacc ctgcaaacca
     1921 gtagcccctc tggacccccc agtgaatgct gagatggagc tgacagcacc gtccctcatc
     1981 cagcccctgg gaatggttcc cctgatcccc agccccttgt catcagcagt gcccctgatc
     2041 ctacctcagg ccccttcagg cccatcctat gccatctacc tgcagcccac tcaagcccac
     2101 caaagtgtga cgccacccca aggcctgagc ccaacggtgt gcaccaccca ctcttctaaa
     2161 gctactggct caaaagactc cacagatgcc accactgaga aggcagccaa tgatacctca
     2221 aaggccagtg cctctaccag gcctggaagc ttgctgccag caccagagag gcaaggggca
     2281 aagagccgaa ccagggagcc agctggagaa agaggctcaa agagggcaag catgctcgag
     2341 gacagtggtt ccaaaaagaa atttaaagag gacctaaaag gacttgaaaa tgtctccgca
     2401 accttgttcc catcaggata cctaatccct ctcacgcagt gctcatccct gggggcagag
     2461 tccattttgt ctggtaaaga aaactcaagt gctctttccc caaaccacag gatttacagc
     2521 tccccaattg caggtgttat tccagtgaca tcatctgaac tcactgctgt taattttccc
     2581 tcttttcatg taacaccgtt gaagctaatg gtctcaccaa cttccgtggc agccgtacct
     2641 gtcgggaaca gcccggctct cgcttcaagc caccctgttc ccatccagaa cccaagctca
     2701 gccattgtaa acttcaccct gcagcacctg ggactcatct cacccaatgt gcagttgtct
     2761 gccagccctg ggtctggaat cgttcctgtg tctccaagaa tagagtctgt taatgtcgca
     2821 ccagaaaatg caggcactca gcaaggaagg gccaccaact atgactcacc agtcccaggc
     2881 cagagccagc caaatggaca atcagttgct gtgacagggg cacaacagcc tgttcctgtg
     2941 acacccaaag ggtcacaatt agtggccgaa agtttcttcc gtaccccagg tggacccacc
     3001 aagccaacca gctcatcctg catggatttt gagggtgcta ataaaacctc cttaggaact
     3061 ctctttgtcc cacagcgaaa actggaagtc tcaacagagg atgtccatta atcaacagat
     3121 gttggcttag tttaattttc taaagagttg tttaatagag aaaatgtaca cagactgatt
     3181 tggagaacac attctctgaa aatactgtaa atacgttggg gatttgttca atgtgaaatc
     3241 agatagttgt tttcatacat atatatatat acacacacac acacacacac acacacacat
     3301 atatttgtat aaagctaagt ttagctttca atcctacaaa ataaaagtaa aatgttgaac
     3361 tctaagatat attaacttct agggggaaaa atccattatt ttagctatgc ctatactatt
     3421 atgcaaagta actgtattaa agtttacttc cctctaagca aatatgcttg acatgcctaa
     3481 cacagcattc ccttaaacat tttgcacaaa gaaaatgctg tgtgatgtat aatgttgtat
     3541 ttttaaatag gggtatagct atattttttg taatttcttt aatctgttgt tgcagtgtat
     3601 ctttttgtaa agtttgcaac aatcctcaat caagtctatg gaaaaattat ttataaaatg
     3661 tatttttaat cataagttgt tcaaattaaa acttttctaa aatata
//
