LOCUS       NM_198968               7491 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens DAZ interacting zinc finger protein 1 (DZIP1),
            transcript variant 2, mRNA.
ACCESSION   NM_198968
VERSION     NM_198968.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Lv,M., Liu,W., Chi,W., Ni,X., Wang,J., Cheng,H., Li,W.Y., Yang,S.,
            Wu,H., Zhang,J., Gao,Y., Liu,C., Li,C., Yang,C., Tan,Q., Tang,D.,
            Zhang,J., Song,B., Chen,Y.J., Li,Q., Zhong,Y., Zhang,Z., Saiyin,H.,
            Jin,L., Xu,Y., Zhou,P., Wei,Z., Zhang,C., He,X., Zhang,F. and
            Cao,Y.
  TITLE     Homozygous mutations in DZIP1 can induce asthenoteratospermia with
            severe MMAF
  JOURNAL   J Med Genet 57 (7), 445-453 (2020)
   PUBMED   32051257
  REMARK    GeneRIF: Homozygous mutations in DZIP1 can induce
            asthenoteratospermia with severe MMAF.
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 7491)
  AUTHORS   Toomer,K.A., Yu,M., Fulmer,D., Guo,L., Moore,K.S., Moore,R.,
            Drayton,K.D., Glover,J., Peterson,N., Ramos-Ortiz,S., Drohan,A.,
            Catching,B.J., Stairley,R., Wessels,A., Lipschutz,J.H.,
            Delling,F.N., Jeunemaitre,X., Dina,C., Collins,R.L., Brand,H.,
            Talkowski,M.E., Del Monte,F., Mukherjee,R., Awgulewitsch,A.,
            Body,S., Hardiman,G., Hazard,E.S., da Silveira,W.A., Wang,B.,
            Leyne,M., Durst,R., Markwald,R.R., Le Scouarnec,S., Hagege,A., Le
            Tourneau,T., Kohl,P., Rog-Zielinska,E.A., Ellinor,P.T.,
            Levine,R.A., Milan,D.J., Schott,J.J., Bouatia-Naji,N.,
            Slaugenhaupt,S.A. and Norris,R.A.
  TITLE     Primary cilia defects causing mitral valve prolapse
  JOURNAL   Sci Transl Med 11 (493) (2019)
   PUBMED   31118289
REFERENCE   4  (bases 1 to 7491)
  AUTHORS   Lee,J.K., Kim,H., Park,Y.M., Kim,D.H. and Lim,H.T.
  TITLE     Mutations in DZIP1 and XYLT1 are associated with nonsyndromic early
            onset high myopia in the Korean population
  JOURNAL   Ophthalmic Genet 38 (4), 395-397 (2017)
   PUBMED   28085539
  REMARK    GeneRIF: In conclusion, we identified two genes, DZIP1 and XYLT1,
            potentially associated with nonsyndromic high myopia using whole
            exome sequencing and subsequent mutation screening analysis.
REFERENCE   5  (bases 1 to 7491)
  AUTHORS   Zhang,B., Wang,G., Xu,X., Yang,S., Zhuang,T., Wang,G., Ren,H.,
            Cheng,S.Y., Jiang,Q. and Zhang,C.
  TITLE     DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like
            Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of
            the BBSome Protein during the Cell Cycle
  JOURNAL   J Biol Chem 292 (4), 1351-1360 (2017)
   PUBMED   27979967
  REMARK    GeneRIF: centrosome protein Dzip1 mediates the assembly of the
            BBSome-Dzip1-PCM1 complex in the centriolar satellites (CS) at the
            G0 phase for ciliary translocation of the BBSome. Phosphorylation
            of Dzip1 at Ser-210 by Plk1 (polo-like kinase 1) during the G2
            phase promotes disassembly of this complex, resulting in removal of
            Dzip1 and the BBSome from the CS.
REFERENCE   6  (bases 1 to 7491)
  AUTHORS   Jin,Z., Mei,W., Strack,S., Jia,J. and Yang,J.
  TITLE     The antagonistic action of B56-containing protein phosphatase 2As
            and casein kinase 2 controls the phosphorylation and Gli turnover
            function of Daz interacting protein 1
  JOURNAL   J Biol Chem 286 (42), 36171-36179 (2011)
   PUBMED   21878643
  REMARK    GeneRIF: reversible phosphorylation of Dzip1, which is controlled
            by the antagonistic action of CK2 and B56-containing PP2As, has an
            important impact on the stability of Gli transcription factors and
            Hh signaling.
REFERENCE   7  (bases 1 to 7491)
  AUTHORS   Glazer,A.M., Wilkinson,A.W., Backer,C.B., Lapan,S.W., Gutzman,J.H.,
            Cheeseman,I.M. and Reddien,P.W.
  TITLE     The Zn finger protein Iguana impacts Hedgehog signaling by
            promoting ciliogenesis
  JOURNAL   Dev Biol 337 (1), 148-156 (2010)
   PUBMED   19852954
REFERENCE   8  (bases 1 to 7491)
  AUTHORS   Moore,F.L., Jaruzelska,J., Dorfman,D.M. and Reijo-Pera,R.A.
  TITLE     Identification of a novel gene, DZIP (DAZ-interacting protein),
            that encodes a protein that interacts with DAZ (deleted in
            azoospermia) and is expressed in embryonic stem cells and germ
            cells
  JOURNAL   Genomics 83 (5), 834-843 (2004)
   PUBMED   15081113
  REMARK    GeneRIF: DZIP may associate with DAZ and its other cofactors in an
            RNA-binding protein complex that functions in both ES cells and
            germ cells
            GeneRIF: gene expression profiling [DZIP]
REFERENCE   9  (bases 1 to 7491)
  AUTHORS   Moore,F.L., Jaruzelska,J., Fox,M.S., Urano,J., Firpo,M.T.,
            Turek,P.J., Dorfman,D.M. and Pera,R.A.
  TITLE     Human Pumilio-2 is expressed in embryonic stem cells and germ cells
            and interacts with DAZ (Deleted in AZoospermia) and DAZ-like
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 538-543 (2003)
   PUBMED   12511597
REFERENCE   10 (bases 1 to 7491)
  AUTHORS   Christian,S.L., McDonough,J., Liu Cy,C.Y., Shaikh,S., Vlamakis,V.,
            Badner,J.A., Chakravarti,A. and Gershon,E.S.
  TITLE     An evaluation of the assembly of an approximately 15-Mb region on
            human chromosome 13q32-q33 linked to bipolar disorder and
            schizophrenia
  JOURNAL   Genomics 79 (5), 635-656 (2002)
   PUBMED   11991713
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA782750.1, DR001764.1, BC041804.1, AK094042.1, BF981831.1,
            AK092363.1 and AI470938.1.
            
            On Jun 2, 2019 this sequence version replaced NM_198968.3.
            
            Transcript Variant: This variant (2) includes an alternate exon in
            the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2) is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.114133.1, BC041804.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376829.7/ ENSP00000366025.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                DA782750.1         1-57
            58-87               DA782750.1         59-88
            88-467              DR001764.1         8-387
            468-3738            BC041804.1         468-3738
            3739-4576           AK094042.1         1703-2540
            4577-4885           BF981831.1         252-560
            4886-7314           AK092363.1         179-2607
            7315-7491           AI470938.1         8-184               c
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.1"
     gene            1..7491
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="DAZ interacting zinc finger protein 1"
                     /db_xref="GeneID:22873"
                     /db_xref="HGNC:HGNC:20908"
                     /db_xref="MIM:608671"
     exon            1..330
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            331..428
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            429..643
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            644..891
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     CDS             856..3459
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="isoform 2 is encoded by transcript variant 2; zinc
                     finger DAZ interacting protein 1; zinc finger protein
                     DZIP1; cilium assembly protein DZIP1; zinc-finger protein
                     DZIPt1; DAZ interacting protein testis1; DAZ-interacting
                     protein 1/2"
                     /codon_start=1
                     /product="cilium assembly protein DZIP1 isoform 2"
                     /protein_id="NP_945319.1"
                     /db_xref="CCDS:CCDS9478.1"
                     /db_xref="GeneID:22873"
                     /db_xref="HGNC:HGNC:20908"
                     /db_xref="MIM:608671"
                     /translation="MQAEAADWFSSMPFQKHVYYPLASGPEGPDVAVAAAAAGAASMA
                     CAPPSAASGPLPFFQFRPRLESVDWRRLSAIDVDKVAGAVDVLTLQENIMNITFCKLE
                     DEKCPHCQSGVDPVLLKLIRLAQFTIEYLLHSQEFLTSQLHTLEERLRLSHCDGEQSK
                     KLLTKQAGEIKTLKEECKRRKKMISTQQLMIEAKANYYQCHFCDKAFMNQAFLQSHIQ
                     RRHTEENSHFEYQKNAQIEKLRSEIVVLKEELQLTRSELEAAHHASAVRFSKEYEMQK
                     TKEEDFLKLFDRWKEEEKEKLVDEMEKVKEMFMKEFKELTSKNSALEYQLSEIQKSNM
                     QIKSNIGTLKDAHEFKEDRSPYPQDFHNVMQLLDSQESKWTARVQAIHQEHKKEKGRL
                     LSHIEKLRTSMIDDLNASNVFYKKRIEELGQRLQEQNELIITQRQQIKDFTCNPLNSI
                     SEPKGNPLAWQAFESQPAAPAVPMNAPALHTLETKSSLPMVHEQAFSSHILEPIEELS
                     EEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGIS
                     SDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDT
                     LSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSS
                     EEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAG
                     VAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLE
                     EEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLV
                     TVTDWSDTSDV"
     exon            892..1452
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1540
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1665
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1827
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1965
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2028
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2169
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2218
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2275
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2332
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2392
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2535
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2698
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..2828
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..2882
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..3073
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3204
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            3205..3379
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     exon            3380..7491
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4556..4561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="hexamer: AGTAAA"
     polyA_site      4577
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="major polyA site"
     regulatory      5815..5820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="hexamer: AATAAA"
     polyA_site      5841
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
     regulatory      7462..7467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
                     /note="hexamer: AATAAA"
     polyA_site      7491
                     /gene="DZIP1"
                     /gene_synonym="DZIP; DZIPt1; MMVP3; MVP3; SPGF47"
ORIGIN      
        1 agaggagccg ggacaccgag ggaagaggcc ggagggagat cgcggagggg agggggcggg
       61 aggggggtgg ggtatcctga gtcgtccgtg gtccggagtc tggaaggccc tagagcggcg
      121 ccagtcgacc cgcctcgcca caacttgccc agaccagaca cgtttcatcc tgcgccctgc
      181 aagaaggagc cggcctgcct ctctccgctt tgctgctcct cggcctccgc gggcccggcc
      241 cgcgtcagca gcgaccctgg ggtctgggtc ccctgtgtcg cccccgcccg cctgcagcgc
      301 ccggcacccg cccaggagcg cgcagctggg gttctaggga cgtatacttg agcaagagag
      361 accacagctc ttgttcccgc tgatcctgca gcccagtgga tggagtccag aatctacaga
      421 cctgccagga aagaaaaaaa aattcctgat gtctggtcca agcaggaatt ccccggtgga
      481 ttggggaatg tctggctttt cctcagctta ttgatctctg tggtaaccac tggaggcccc
      541 cagaagaccc atagaaatga gaggcctgag gtctacaagt cgctgctaga aatattttag
      601 cctctccaaa gcccagaatg cagccccgac ccaagtttgt aagggttctg ggtgcacgct
      661 gaccctgcgc gggcagacgc gccctttgct ccaggtccgg acctgggcgc tgctatagca
      721 acgtcctgga cgcccagacc ttaggccgcc gccgccgcgg aagcgaggaa cccggccttc
      781 tcccgctcct gagggctgtg gcggcggcgg cccgggaggc ggcccaggct gggtaaagac
      841 cgcccggctc ctcctatgca agctgaggca gcggattggt tttcaagcat gcccttccag
      901 aagcatgtct actacccgct cgccagcggc ccagaggggc ccgacgtcgc tgtggccgcc
      961 gccgccgcgg gtgcggcctc catggcctgt gcgcccccca gcgcggcttc ggggcccctg
     1021 cccttcttcc agttcaggcc gcggctggag agtgtggact ggcggcggct gagcgccatc
     1081 gacgtggaca aggtggcggg ggctgtggac gtgctgacgc tgcaggagaa catcatgaac
     1141 atcaccttct gcaagctgga agacgagaag tgcccacact gccagtcggg ggtggacccg
     1201 gtgctgctga agctcatccg tctggcgcag ttcaccatcg agtacttgct gcactcacaa
     1261 gagttcctca cctcgcagct gcacaccctg gaggagcggc tgcgcctgag ccactgcgac
     1321 ggcgagcaga gcaagaagct gctcaccaag caggcggggg agatcaagac gctcaaggaa
     1381 gagtgcaaac gccggaagaa gatgatctcc acccagcagc tgatgatcga ggccaaagcc
     1441 aactattacc agtgccattt ttgtgacaag gcctttatga accaagcttt tctacaaagt
     1501 cacattcaac gccgccacac tgaagaaaat tctcattttg agtatcagaa aaatgcacag
     1561 attgagaagc tccggagtga gatcgtcgta ttgaaggaag agctgcagct caccaggtct
     1621 gagctagagg ctgcacacca tgccagtgca gtcagattct ccaaggaata tgaaatgcag
     1681 aaaacaaaag aggaagactt tttgaagtta tttgacaggt ggaaagaaga agaaaaggag
     1741 aaactagttg atgaaatgga aaaagtcaag gagatgttta tgaaggaatt taaagaatta
     1801 acttcgaaga attcagcatt agaatatcaa ctgtcagaaa tccagaagtc caatatgcag
     1861 atcaagtcca acataggcac attaaaagat gcacacgagt ttaaagaaga ccgttctcca
     1921 tatccccagg atttccataa tgtcatgcag cttcttgata gtcaggaaag caaatggaca
     1981 gctcgagttc aagctattca tcaagaacac aagaaagaga agggtcggct cctgtcacat
     2041 atagagaaac ttcgaacctc aatgatagat gatctaaatg caagcaatgt tttctataag
     2101 aaaaggatag aagagctagg gcagagactc caggagcaga atgagctgat tataactcag
     2161 agacagcaga ttaaagactt tacctgtaat ccattaaaca gtatcagtga acccaaagga
     2221 aatcctttag cctggcaggc ttttgaatct cagccagctg ctccagctgt gcctatgaat
     2281 gccccagccc tgcacacttt ggaaactaaa tcaagtctgc caatggtgca tgaacaggca
     2341 ttctcgtcgc acatactgga accaatagaa gaactttcag aggaagaaaa aggaagggaa
     2401 aatgaacaga aattaaataa caacaaaatg catttaagga aagctttgaa gagtaactcc
     2461 tccctcacta agggactaag aacaatggtg gagcagaact tgatggagaa actggaaacc
     2521 ttggggatta atgcagatat acgtggcatt tcaagtgatc agttgcatag agtactaaaa
     2581 agtgtggaat cagaaagaca taagcaagaa agagaaatac ctaactttca tcaaattcga
     2641 gaattccttg aacatcaagt cagctgtaaa attgaggaga aagcactact ctcttcagat
     2701 cagtgcagtg tttctcaaat ggataccctt tcaactggag aagtacccaa aatgatacaa
     2761 cttccttcca aaaacagaca actgattaga caaaaagctg tttctactga taggacatct
     2821 gttccaaaaa ttaagaaaaa tgtcatggaa gatccttttc ccagaaagtc ttcaactatt
     2881 acgacccctc cttttagttc agaggaggag caggaggacg acgacctcat ccgggcatac
     2941 gcatccccag gcccacttcc tgtgccgcca ccacaaaaca agggcagctt cgggaagaac
     3001 acagtgaaaa gtgacgcgga cgggaccgag ggaagcgaaa tcgaggacac tgatgattct
     3061 cccaagcccg caggagtcgc cgttaaaaca cctactgaaa aagttgaaaa gatgtttcca
     3121 catcgcaaaa atgtgaacaa accagtcggt ggaactaatg tccctgagat gtttatcaaa
     3181 aaagaagaat tacaagaact aaagtgtgcg gatgtggagg atgaagactg ggacatatca
     3241 tccctagagg aagagatatc tttgggaaaa aaatctggga aagaacagaa ggaacctcca
     3301 cctgcgaaaa atgaaccaca ttttgctcat gtgctaaatg cctggggcgc atttaatcct
     3361 aaggggccaa agggagaagg acttcaagaa aatgaatcaa gcacattaaa aagcagctta
     3421 gtaactgtga ctgattggag cgacacttca gatgtctaat tccacatgtc agaagattat
     3481 tccagaagcc agcagtattt cagtatcaca gtgtttcagt aatttgcctc catgattcta
     3541 gtgcttctgc cttaccgtgt ttcccacagc aacacagaga ctgattcaaa gaacaatggt
     3601 ctctttaatg gcacccaata cagtattgaa aatcagatca tcaacagtat ttcgaagcat
     3661 gtaaaggtgt ttaagacttc cgctgctgct taaaaataac atgtcattga agtcataaaa
     3721 agttttttct tcagaaaggt actctagtgt taagtgtatt tttttcaact aattttttag
     3781 tgaatttttt ttaaacttac agcatgtttt ggtttgaatt actaaaactt taaaaaatat
     3841 ttttcttatg tatgctgtcg tatcgtaggc gtttatatta taaaattctg ttagtagtct
     3901 taaaattgaa ttggtggaac cactaatcct taaaagttag tctggttatt tttcatatag
     3961 aagtaagttt aatccgagtg tggtggtgtt cacctttaat cccagctact tgggaggctg
     4021 aggtgggagg ataacttgag cacaggagtt caagaccagc gtgggcaata tagcaagact
     4081 ccacccctcc acaccccaaa aaagtaagtt taggattaga atatagctag gtccaatgtt
     4141 aaatacattt tcctggagta catttgtcac attcagcttt gagccactgt aagcatgtta
     4201 ctattaaatg gttggttatt ttatatagca tattctttat cttggatatt ttatgaataa
     4261 agtatagtta ttttaagtgc caattaattt atcagactaa atagaaaata tttgagccat
     4321 tactgaattc acatatgtat gttttttttt actatttaaa atacccaaca tgtattatga
     4381 aatacctcaa aagtaattta gttacattct taaacaatga cattgtcgaa agaaagttct
     4441 tataagctgt tttttgcatt tttataactt ggttatacta tattctgttt ccaagtaacc
     4501 ttttaactaa aagatttgtt gggttttaga tctcttttca tttgtcaacc ttttcagtaa
     4561 agccctctgt tacatcactg tgactgtggc tgtgttatta gagatgaatt ctgagttaaa
     4621 tttgtactta ttaggggtct tacagaggga gaatctggcc ttgattgcta tgccatatga
     4681 cattctgtcc taatggttag aacaaatctt tatctgtttc cacccaccct gggatccttt
     4741 ttacttgtag cccattcctg ccctctaaaa gaatccttcc aacttcctat aggggccatt
     4801 tcatgcagat atactcactt tatatgtatg tttctaaaag ctttagtgaa ggctgaatcc
     4861 aagtttcatg tagtaggaca gagaacagaa ggccgaatcc aagtttcatg tggtaggaca
     4921 gagaacggca atctacaaaa ctacaccaag cctaagatgg cccatgtgcc actttgttca
     4981 ttcatttatt tattcagcag atatttattg aaccacttgg tctggtttgg ttttgaacac
     5041 gcactaattc atttaattac aacaactttg cgatgtagtt attgttataa tcctcatcct
     5101 aaaggtgtga gaaacagagt tattaagtaa gttgccagaa gccatacaag tacaaagtgt
     5161 caaggggaga ctttaagcca ggtctctatt ccatgttttg tgtctgccat gttttcattc
     5221 ctgagcagaa gagcttgggg aaatgaactt ttgctaagta aacccagtat ttaaattaag
     5281 tcactatatc tgtgtctttt aagagcccct cctttagctt acatggttag ttgtatcttt
     5341 tttttttttt ttttttgaga cagagtctca ctatgttgcc caggcttgag cgcagtggca
     5401 ccatctcggc tcactacaac ctccgcctcc cagattcaag cgattttcct gcctcagcct
     5461 cccaagtagc tgggattaca ggtgcccacc accatgcctg gcttaatttt ttgtattttt
     5521 agtagagatg aggtttcacc atgttggcca gactggtctc gaactcctga cctcaaatga
     5581 tccgctgtct tgtcctccca aagtgctggg attataggcg tgagccatcg cgccctgctg
     5641 catttcattt ttttgtataa aatttggttt gttcgttttt gaggggaacc attcctttat
     5701 gttgcccctt actgatgccc aactaatgtg tttctggtta ctaacagacc ttcatccccc
     5761 accctcccta aattcttcgg tgtaatctaa gcgttatctg caccacttct gaataataaa
     5821 tgtgtttgaa atgcacacct acacaaccct gtcccacact ggcctgtagg acctaatcta
     5881 ccttcatgtc tttcagttga tttgtatttc tgcaaaagat atttcaagga ttatcaaggt
     5941 atggaatttt ttttattttt tatcttttaa caaacttgtt catttccttt tctttccatt
     6001 gagcacctct gaacaatgtg attctttttt ttaagtcttt cacacttcag gaaaatacta
     6061 cattcaccca cgttgtgatt catatcggac agtttcttac tcaaagataa cacaaatacc
     6121 aattcttatt catactttga aactatattt taccctagac tgccttggtt caaaggccta
     6181 gtttcgacat tactgtggca aattatttaa gctctctgtt ttcatttctt tttctgtaaa
     6241 tgcaggatga aaacaatatg tatctcctag gatggctgtg aggagtgagt gagacaggac
     6301 atgaaaggtg tgtatgaact gtgataactg ttgcccttgt gggcatgtta tatagggctg
     6361 gctctaggaa gaagcctgtg tcttttttca gaaatccctg gagcaactta aatgtcttta
     6421 gaaatgacaa gtctctcagt tatccagtta gccgtaaaca ggttctggaa gacacaccta
     6481 gatagaactc ggcccgtcag ttctgagtga ggcacgtacc acttgggctt gggatgtcct
     6541 taacccattt atgcctgagg ttgcaatttt ttgaattttt ccaatcacac cttggcgatg
     6601 accttgagca gtaggatata aataactccc acatgcttag tgttccaata atggaacact
     6661 aggcataaac aggttttaat tggctcctca gtcaaccatg accaatatct ctatctcagc
     6721 ttctgctgat gccctagctc agggctctgc attccgtgcc agggacgcct cagcccctta
     6781 acatggatgt gccacatgac aggcaataat atattggccc cattagccct tcctggagca
     6841 gtcataatga attacagtca tgacaggcac atgtaagaaa ccaaaatttg ctaaatatgt
     6901 acatgagtct tcatcacagc atcgtttatg aaaacaacta aatattcact aatggtgcca
     6961 gtggaataaa tcagagaaca tcccctgcta cgtaactctc tgcatacatc aaagagaatg
     7021 gtgtggcttt gctttttcaa caatctactg agtggccatg ggcatgtgga tatggccatg
     7081 aatgagcaag atcctctctg atcctgtaga agttaagttc taccagataa cttgctgctt
     7141 caacaaaaag atttaccttt ttaaataaat gttgtagaat acttaaaaaa aacaaactag
     7201 aatttgcctg tgtgcagcca gtaacatgtc tatttaacct ggacaccttt tgaggaatat
     7261 tctcagattg cccccatgct gtttataaga cattgttcct tatacacctg tttatgaatg
     7321 aaaagaaaca taaggagtgg gtacaaagac ttctatctat gaatgattaa aaaggctaga
     7381 gtacgaatac ttcttgaacc tttggtacta aatgcttttc atgttctata taaatgtaga
     7441 aaacatttta caaatcctgt aaataaactg tttatttttt atagaaagcc a
//
