LOCUS       NM_152773                625 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens dynein light chain Tctex-type 2B (DYNLT2B), transcript
            variant 2, mRNA.
ACCESSION   NM_152773 XM_936384
VERSION     NM_152773.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 625)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 625)
  AUTHORS   Hamada,Y., Tsurumi,Y., Nozaki,S., Katoh,Y. and Nakayama,K.
  TITLE     Interaction of WDR60 intermediate chain with TCTEX1D2 light chain
            of the dynein-2 complex is crucial for ciliary protein trafficking
  JOURNAL   Mol Biol Cell 29 (13), 1628-1639 (2018)
   PUBMED   29742051
  REMARK    GeneRIF: WDR60 plays a major role and TCTEX1D2 plays an auxiliary
            role in the dynein-2 complex to mediate retrograde ciliary protein
            trafficking.
REFERENCE   4  (bases 1 to 625)
  AUTHORS   Boldt,K., van Reeuwijk,J., Lu,Q., Koutroumpas,K., Nguyen,T.M.,
            Texier,Y., van Beersum,S.E., Horn,N., Willer,J.R., Mans,D.A.,
            Dougherty,G., Lamers,I.J., Coene,K.L., Arts,H.H., Betts,M.J.,
            Beyer,T., Bolat,E., Gloeckner,C.J., Haidari,K., Hetterschijt,L.,
            Iaconis,D., Jenkins,D., Klose,F., Knapp,B., Latour,B.,
            Letteboer,S.J., Marcelis,C.L., Mitic,D., Morleo,M., Oud,M.M.,
            Riemersma,M., Rix,S., Terhal,P.A., Toedt,G., van Dam,T.J., de
            Vrieze,E., Wissinger,Y., Wu,K.M., Apic,G., Beales,P.L.,
            Blacque,O.E., Gibson,T.J., Huynen,M.A., Katsanis,N., Kremer,H.,
            Omran,H., van Wijk,E., Wolfrum,U., Kepes,F., Davis,E.E., Franco,B.,
            Giles,R.H., Ueffing,M., Russell,R.B. and Roepman,R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 625)
  AUTHORS   Schmidts,M., Hou,Y., Cortes,C.R., Mans,D.A., Huber,C., Boldt,K.,
            Patel,M., van Reeuwijk,J., Plaza,J.M., van Beersum,S.E., Yap,Z.M.,
            Letteboer,S.J., Taylor,S.P., Herridge,W., Johnson,C.A.,
            Scambler,P.J., Ueffing,M., Kayserili,H., Krakow,D., King,S.M.,
            Beales,P.L., Al-Gazali,L., Wicking,C., Cormier-Daire,V.,
            Roepman,R., Mitchison,H.M. and Witman,G.B.
  CONSRTM   UK10K
  TITLE     TCTEX1D2 mutations underlie Jeune asphyxiating thoracic dystrophy
            with impaired retrograde intraflagellar transport
  JOURNAL   Nat Commun 6, 7074 (2015)
   PUBMED   26044572
  REMARK    GeneRIF: integral component of the evolutionarily conserved
            retrograde intraflagellar transport machinery
            Erratum:[Nat Commun. 2016 Mar 29;7:11270. doi:
            10.1038/ncomms11270.. PMID: 27021811]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 625)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   7  (bases 1 to 625)
  AUTHORS   Gholkar,A.A., Senese,S., Lo,Y.C., Capri,J., Deardorff,W.J.,
            Dharmarajan,H., Contreras,E., Hodara,E., Whitelegge,J.P.,
            Jackson,P.K. and Torres,J.Z.
  TITLE     Tctex1d2 associates with short-rib polydactyly syndrome proteins
            and is required for ciliogenesis
  JOURNAL   Cell Cycle 14 (7), 1116-1125 (2015)
   PUBMED   25830415
  REMARK    GeneRIF: Tctex1d2 is a novel dynein light chain important for
            trafficking to the cilium and potentially retrograde intraflagellar
            transport.
REFERENCE   8  (bases 1 to 625)
  AUTHORS   Asante,D., Stevenson,N.L. and Stephens,D.J.
  TITLE     Subunit composition of the human cytoplasmic dynein-2 complex
  JOURNAL   J Cell Sci 127 (Pt 21), 4774-4787 (2014)
   PUBMED   25205765
REFERENCE   9  (bases 1 to 625)
  AUTHORS   Miyamoto-Sato,E., Fujimori,S., Ishizaka,M., Hirai,N., Masuoka,K.,
            Saito,R., Ozawa,Y., Hino,K., Washio,T., Tomita,M., Yamashita,T.,
            Oshikubo,T., Akasaka,H., Sugiyama,J., Matsumoto,Y. and Yanagawa,H.
  TITLE     A comprehensive resource of interacting protein regions for
            refining human transcription factor networks
  JOURNAL   PLoS One 5 (2), e9289 (2010)
   PUBMED   20195357
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC021177.2, BM548106.1 and
            BM983853.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_152773.4.
            
            Summary: Dyneins are a group of microtubule-activated ATPases that
            function as molecular motors. They are divided into two subgroups
            of axonemal and cytoplasmic dyneins. The cytoplasmic dyneins
            function in intracellular motility, including retrograde axonal
            transport, protein sorting, organelle movement, and spindle
            dynamics. Molecules of conventional cytoplasmic dynein are
            comprised of 2 heavy chain polypeptides and a number of
            intermediate and light chains. This gene encodes a subunit of the
            human cytoplasmic dynein-2 complex. Mutations in this gene are
            associated with short-rib thoracic dysplasia 17 with or without
            polydactyly. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, May 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.542246.1,
                                           SRR1163657.100823.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325318.10/ ENSP00000324323.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-251               BC021177.2         35-285
            252-335             BM548106.1         331-414
            336-617             BC021177.2         370-651
            618-625             BM983853.1         17-24               c
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..625
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /note="dynein light chain Tctex-type 2B"
                     /db_xref="GeneID:255758"
                     /db_xref="HGNC:HGNC:28482"
                     /db_xref="MIM:617353"
     exon            1..201
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..517
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tctex1 domain-containing protein 2; Tctex1 domain
                     containing 2; dynein light chain Tctex-type protein 2B"
                     /codon_start=1
                     /product="dynein light chain Tctex-type protein 2B isoform
                     2"
                     /protein_id="NP_689986.2"
                     /db_xref="CCDS:CCDS33929.1"
                     /db_xref="GeneID:255758"
                     /db_xref="HGNC:HGNC:28482"
                     /db_xref="MIM:617353"
                     /translation="MATSIGVSFSVGDGVPEAEKNAGEPENTYILRPVFQQRFRPSVV
                     KDCIHAVLKEELANAEYSPEEMPQLTKHLSENIKDKLKEMGFDRYKMVVQVVIGEQRG
                     EGVFMASRCFWDADTDNYTHDVFMNDSLFCVVAAFGCFYY"
     exon            202..335
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..405
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            406..469
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            470..625
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      596..601
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /note="hexamer: AATAAA"
     polyA_site      625
                     /gene="DYNLT2B"
                     /gene_synonym="SRTD17; TCTEX1D2"
                     /note="major polyA site"
ORIGIN      
        1 gccctggaga cgctttccct gctgccggcc gcgaccgcaa cccgctaggc cttcatcgcg
       61 agctacgccc ggaccgagaa gccccggcat ggccacgtcc atcggagtgt ccttctcggt
      121 gggcgacggg gtgcctgagg ctgagaagaa cgcaggggag cccgagaaca cctatattct
      181 gcggcctgtt ttccagcaga ggttcaggcc ctctgtggtt aaagactgta tccatgctgt
      241 gctcaaggag gaactggcaa atgctgaata ttctccagaa gaaatgcctc agcttacaaa
      301 acatttatca gaaaacatta aagataaatt aaaagaaatg ggatttgacc gatacaaaat
      361 ggtggtgcaa gtagtgattg gagaacaaag aggtgaagga gtattcatgg cttctcgctg
      421 tttctgggat gctgacactg acaactatac tcatgatgtt ttcatgaatg acagtttatt
      481 ctgcgttgta gcagcatttg gctgtttcta ctactgaatg aatctttgaa aagctggtaa
      541 aagacatgac catgaagaaa tctgaacttt ttaatattgt taaatatctt gacaaaataa
      601 agatgttagt agtttgacaa ctgaa
//
