LOCUS       NM_014183                662 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens dynein light chain roadblock-type 1 (DYNLRB1),
            transcript variant 1, mRNA.
ACCESSION   NM_014183
VERSION     NM_014183.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 662)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 662)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 662)
  AUTHORS   Tsurumi,Y., Hamada,Y., Katoh,Y. and Nakayama,K.
  TITLE     Interactions of the dynein-2 intermediate chain WDR34 with the
            light chains are required for ciliary retrograde protein
            trafficking
  JOURNAL   Mol Biol Cell 30 (5), 658-670 (2019)
   PUBMED   30649997
REFERENCE   5  (bases 1 to 662)
  AUTHORS   Schlager,M.A., Hoang,H.T., Urnavicius,L., Bullock,S.L. and
            Carter,A.P.
  TITLE     In vitro reconstitution of a highly processive recombinant human
            dynein complex
  JOURNAL   EMBO J 33 (17), 1855-1868 (2014)
   PUBMED   24986880
REFERENCE   6  (bases 1 to 662)
  AUTHORS   Pfister,K.K., Fisher,E.M., Gibbons,I.R., Hays,T.S., Holzbaur,E.L.,
            McIntosh,J.R., Porter,M.E., Schroer,T.A., Vaughan,K.T.,
            Witman,G.B., King,S.M. and Vallee,R.B.
  TITLE     Cytoplasmic dynein nomenclature
  JOURNAL   J Cell Biol 171 (3), 411-413 (2005)
   PUBMED   16260502
REFERENCE   7  (bases 1 to 662)
  AUTHORS   Tang,Q., Staub,C.M., Gao,G., Jin,Q., Wang,Z., Ding,W.,
            Aurigemma,R.E. and Mulder,K.M.
  TITLE     A novel transforming growth factor-beta receptor-interacting
            protein that is also a light chain of the motor protein dynein
  JOURNAL   Mol Biol Cell 13 (12), 4484-4496 (2002)
   PUBMED   12475967
REFERENCE   8  (bases 1 to 662)
  AUTHORS   Susalka,S.J., Nikulina,K., Salata,M.W., Vaughan,P.S., King,S.M.,
            Vaughan,K.T. and Pfister,K.K.
  TITLE     The roadblock light chain binds a novel region of the cytoplasmic
            Dynein intermediate chain
  JOURNAL   J Biol Chem 277 (36), 32939-32946 (2002)
   PUBMED   12077152
REFERENCE   9  (bases 1 to 662)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Ray,S., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human retina for the NEIBank
            Project: retbindin, an abundant, novel retinal cDNA and alternative
            splicing of other retina-preferred gene transcripts
  JOURNAL   Mol Vis 8, 196-204 (2002)
   PUBMED   12107411
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 662)
  AUTHORS   Jiang,J., Yu,L., Huang,X., Chen,X., Li,D., Zhang,Y., Tang,L. and
            Zhao,S.
  TITLE     Identification of two novel human dynein light chain genes, DNLC2A
            and DNLC2B, and their expression changes in hepatocellular
            carcinoma tissues from 68 Chinese patients
  JOURNAL   Gene 281 (1-2), 103-113 (2001)
   PUBMED   11750132
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109923.29 and AL136173.24.
            
            On May 1, 2020 this sequence version replaced NM_014183.3.
            
            Summary: This gene is a member of the roadblock dynein light chain
            family. The encoded cytoplasmic protein is capable of binding
            intermediate chain proteins, interacts with transforming growth
            factor-beta, and has been implicated in the regulation of actin
            modulating proteins. Upregulation of this gene has been associated
            with hepatocellular carcinomas, suggesting that this gene may be
            involved in tumor progression. Alternative splicing results in
            multiple transcript variants. Pseudogenes of this gene have been
            defined on chromosomes 12 and 18. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1), as well as variant 8, encodes
            isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA734845.1, BQ056781.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357156.7/ ENSP00000349679.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AL109923.29        93564-93603
            41-116              AL136173.24        5678-5753
            117-284             AL136173.24        14037-14204
            285-662             AL136173.24        19990-20367
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22"
     gene            1..662
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /note="dynein light chain roadblock-type 1"
                     /db_xref="GeneID:83658"
                     /db_xref="HGNC:HGNC:15468"
                     /db_xref="MIM:607167"
     exon            1..40
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..328
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /note="isoform a is encoded by transcript variant 1;
                     dynein, cytoplasmic, light polypeptide 2A;
                     bithoraxoid-like protein; dynein-associated protein Km23;
                     ROBL/LC7-like 1; roadblock domain-containing protein 1"
                     /codon_start=1
                     /product="dynein light chain roadblock-type 1 isoform a"
                     /protein_id="NP_054902.1"
                     /db_xref="CCDS:CCDS13235.1"
                     /db_xref="GeneID:83658"
                     /db_xref="HGNC:HGNC:15468"
                     /db_xref="MIM:607167"
                     /translation="MAEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYAS
                     LMHSFILKARSTVRDIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPTE"
     exon            41..116
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /inference="alignment:Splign:2.1.0"
     exon            117..284
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /inference="alignment:Splign:2.1.0"
     exon            285..662
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      640..645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /note="hexamer: ACTAAA"
     polyA_site      662
                     /gene="DYNLRB1"
                     /gene_synonym="BITH; BLP; DNCL2A; DNLC2A; ROBLD1"
                     /note="major polyA site"
ORIGIN      
        1 aagtgttcgc tacgcggggc taccggatcg gtcggaaatg gcagaggtgg aggagacact
       61 gaagcgactg cagagccaga agggagtgca gggaatcatc gtcgtgaaca cagaaggcat
      121 tcccatcaag agcaccatgg acaaccccac caccacccag tatgccagcc tcatgcacag
      181 cttcatcctg aaggcacgga gcaccgtgcg tgacatcgac ccccagaacg atctcacctt
      241 ccttcgaatt cgctccaaga aaaatgaaat tatggttgca ccagataaag actatttcct
      301 gattgtgatt cagaatccaa ccgaataagc cactctcttg gctccctgtg tcattcctta
      361 atttaatgcc ccccaagaat gttaatgtca atcatgtcag tggactagca catggcagtc
      421 gcttggaacc cactcacacc aatccagtga ccgtgtgtgg gctggcggct cttctccccc
      481 accaacggaa cccctgtgtg caccaacctt ccccagagct ccggagcgcc ctctcctcac
      541 ttccaggttt tggagcaaga gcttgcagga agcccgcacc cagcttcctt ctgaccttca
      601 gttcactttg tcgcccttgg agaaagctgt ttttctttaa ctaaaaataa ccaaaatgct
      661 ta
//
