LOCUS       NM_001374441            9574 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens dymeclin (DYM), transcript variant 22, mRNA.
ACCESSION   NM_001374441
VERSION     NM_001374441.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Abdullah, Shah,P.W., Nawaz,S., Hussain,S., Ullah,A., Basit,S. and
            Ahmad,W.
  TITLE     A homozygous nonsense variant in DYM underlies
            Dyggve-Melchior-Clausen syndrome associated with ectodermal
            features
  JOURNAL   Mol Biol Rep 47 (9), 7083-7088 (2020)
   PUBMED   32886330
  REMARK    GeneRIF: A homozygous nonsense variant in DYM underlies
            Dyggve-Melchior-Clausen syndrome associated with ectodermal
            features.
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Dupuis,N., Fafouri,A., Bayot,A., Kumar,M., Lecharpentier,T.,
            Ball,G., Edwards,D., Bernard,V., Dournaud,P., Drunat,S.,
            Vermelle-Andrzejewski,M., Vilain,C., Abramowicz,M., Desir,J.,
            Bonaventure,J., Gareil,N., Boncompain,G., Csaba,Z., Perez,F.,
            Passemard,S., Gressens,P. and El Ghouzzi,V.
  TITLE     Dymeclin deficiency causes postnatal microcephaly, hypomyelination
            and reticulum-to-Golgi trafficking defects in mice and humans
  JOURNAL   Hum Mol Genet 24 (10), 2771-2783 (2015)
   PUBMED   25652408
  REMARK    GeneRIF: Dymeclin is crucial for proper myelination and anterograde
            neuronal trafficking, two processes that are highly active during
            postnatal brain maturation.
REFERENCE   3  (bases 1 to 9574)
  AUTHORS   He,M., Xu,M., Zhang,B., Liang,J., Chen,P., Lee,J.Y., Johnson,T.A.,
            Li,H., Yang,X., Dai,J., Liang,L., Gui,L., Qi,Q., Huang,J., Li,Y.,
            Adair,L.S., Aung,T., Cai,Q., Cheng,C.Y., Cho,M.C., Cho,Y.S.,
            Chu,M., Cui,B., Gao,Y.T., Go,M.J., Gu,D., Gu,W., Guo,H., Hao,Y.,
            Hong,J., Hu,Z., Hu,Y., Huang,J., Hwang,J.Y., Ikram,M.K., Jin,G.,
            Kang,D.H., Khor,C.C., Kim,B.J., Kim,H.T., Kubo,M., Lee,J., Lee,J.,
            Lee,N.R., Li,R., Li,J., Liu,J., Longe,J., Lu,W., Lu,X., Miao,X.,
            Okada,Y., Ong,R.T., Qiu,G., Seielstad,M., Sim,X., Song,H.,
            Takeuchi,F., Tanaka,T., Taylor,P.R., Wang,L., Wang,W., Wang,Y.,
            Wu,C., Wu,Y., Xiang,Y.B., Yamamoto,K., Yang,H., Liao,M., Yokota,M.,
            Young,T., Zhang,X., Kato,N., Wang,Q.K., Zheng,W., Hu,F.B., Lin,D.,
            Shen,H., Teo,Y.Y., Mo,Z., Wong,T.Y., Lin,X., Mohlke,K.L., Ning,G.,
            Tsunoda,T., Han,B.G., Shu,X.O., Tai,E.S., Wu,T. and Qi,L.
  TITLE     Meta-analysis of genome-wide association studies of adult height in
            East Asians identifies 17 novel loci
  JOURNAL   Hum Mol Genet 24 (6), 1791-1800 (2015)
   PUBMED   25429064
REFERENCE   4  (bases 1 to 9574)
  AUTHORS   Seven,M., Koparir,E., Gezdirici,A., Aydin,H., Skladny,H.,
            Fenercioglu,E., Guven,G., Karatas,O.F., Koparir,A., Ozen,M. and
            Ulucan,H.
  TITLE     A novel frameshift mutation and infrequent clinical findings in two
            cases with Dyggve-Melchior-Clausen syndrome
  JOURNAL   Clin Dysmorphol 23 (1), 1-7 (2014)
   PUBMED   24300288
  REMARK    GeneRIF: The clinical diagnosis was confirmed with molecular
            analysis of DYM with a known mutation at c.580C>T (p.R194X).
REFERENCE   5  (bases 1 to 9574)
  AUTHORS   Berndt,S.I., Gustafsson,S., Magi,R., Ganna,A., Wheeler,E.,
            Feitosa,M.F., Justice,A.E., Monda,K.L., Croteau-Chonka,D.C.,
            Day,F.R., Esko,T., Fall,T., Ferreira,T., Gentilini,D.,
            Jackson,A.U., Luan,J., Randall,J.C., Vedantam,S., Willer,C.J.,
            Winkler,T.W., Wood,A.R., Workalemahu,T., Hu,Y.J., Lee,S.H.,
            Liang,L., Lin,D.Y., Min,J.L., Neale,B.M., Thorleifsson,G., Yang,J.,
            Albrecht,E., Amin,N., Bragg-Gresham,J.L., Cadby,G., den Heijer,M.,
            Eklund,N., Fischer,K., Goel,A., Hottenga,J.J., Huffman,J.E.,
            Jarick,I., Johansson,A., Johnson,T., Kanoni,S., Kleber,M.E.,
            Konig,I.R., Kristiansson,K., Kutalik,Z., Lamina,C., Lecoeur,C.,
            Li,G., Mangino,M., McArdle,W.L., Medina-Gomez,C.,
            Muller-Nurasyid,M., Ngwa,J.S., Nolte,I.M., Paternoster,L.,
            Pechlivanis,S., Perola,M., Peters,M.J., Preuss,M., Rose,L.M.,
            Shi,J., Shungin,D., Smith,A.V., Strawbridge,R.J., Surakka,I.,
            Teumer,A., Trip,M.D., Tyrer,J., Van Vliet-Ostaptchouk,J.V.,
            Vandenput,L., Waite,L.L., Zhao,J.H., Absher,D., Asselbergs,F.W.,
            Atalay,M., Attwood,A.P., Balmforth,A.J., Basart,H., Beilby,J.,
            Bonnycastle,L.L., Brambilla,P., Bruinenberg,M., Campbell,H.,
            Chasman,D.I., Chines,P.S., Collins,F.S., Connell,J.M.,
            Cookson,W.O., de Faire,U., de Vegt,F., Dei,M., Dimitriou,M.,
            Edkins,S., Estrada,K., Evans,D.M., Farrall,M., Ferrario,M.M.,
            Ferrieres,J., Franke,L., Frau,F., Gejman,P.V., Grallert,H.,
            Gronberg,H., Gudnason,V., Hall,A.S., Hall,P., Hartikainen,A.L.,
            Hayward,C., Heard-Costa,N.L., Heath,A.C., Hebebrand,J., Homuth,G.,
            Hu,F.B., Hunt,S.E., Hypponen,E., Iribarren,C., Jacobs,K.B.,
            Jansson,J.O., Jula,A., Kahonen,M., Kathiresan,S., Kee,F.,
            Khaw,K.T., Kivimaki,M., Koenig,W., Kraja,A.T., Kumari,M.,
            Kuulasmaa,K., Kuusisto,J., Laitinen,J.H., Lakka,T.A.,
            Langenberg,C., Launer,L.J., Lind,L., Lindstrom,J., Liu,J.,
            Liuzzi,A., Lokki,M.L., Lorentzon,M., Madden,P.A., Magnusson,P.K.,
            Manunta,P., Marek,D., Marz,W., Mateo Leach,I., McKnight,B.,
            Medland,S.E., Mihailov,E., Milani,L., Montgomery,G.W., Mooser,V.,
            Muhleisen,T.W., Munroe,P.B., Musk,A.W., Narisu,N., Navis,G.,
            Nicholson,G., Nohr,E.A., Ong,K.K., Oostra,B.A., Palmer,C.N.,
            Palotie,A., Peden,J.F., Pedersen,N., Peters,A., Polasek,O.,
            Pouta,A., Pramstaller,P.P., Prokopenko,I., Putter,C.,
            Radhakrishnan,A., Raitakari,O., Rendon,A., Rivadeneira,F.,
            Rudan,I., Saaristo,T.E., Sambrook,J.G., Sanders,A.R., Sanna,S.,
            Saramies,J., Schipf,S., Schreiber,S., Schunkert,H., Shin,S.Y.,
            Signorini,S., Sinisalo,J., Skrobek,B., Soranzo,N., Stancakova,A.,
            Stark,K., Stephens,J.C., Stirrups,K., Stolk,R.P., Stumvoll,M.,
            Swift,A.J., Theodoraki,E.V., Thorand,B., Tregouet,D.A., Tremoli,E.,
            Van der Klauw,M.M., van Meurs,J.B., Vermeulen,S.H., Viikari,J.,
            Virtamo,J., Vitart,V., Waeber,G., Wang,Z., Widen,E., Wild,S.H.,
            Willemsen,G., Winkelmann,B.R., Witteman,J.C., Wolffenbuttel,B.H.,
            Wong,A., Wright,A.F., Zillikens,M.C., Amouyel,P., Boehm,B.O.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Chanock,S.J.,
            Cupples,L.A., Cusi,D., Dedoussis,G.V., Erdmann,J., Eriksson,J.G.,
            Franks,P.W., Froguel,P., Gieger,C., Gyllensten,U., Hamsten,A.,
            Harris,T.B., Hengstenberg,C., Hicks,A.A., Hingorani,A., Hinney,A.,
            Hofman,A., Hovingh,K.G., Hveem,K., Illig,T., Jarvelin,M.R.,
            Jockel,K.H., Keinanen-Kiukaanniemi,S.M., Kiemeney,L.A., Kuh,D.,
            Laakso,M., Lehtimaki,T., Levinson,D.F., Martin,N.G., Metspalu,A.,
            Morris,A.D., Nieminen,M.S., Njolstad,I., Ohlsson,C.,
            Oldehinkel,A.J., Ouwehand,W.H., Palmer,L.J., Penninx,B., Power,C.,
            Province,M.A., Psaty,B.M., Qi,L., Rauramaa,R., Ridker,P.M.,
            Ripatti,S., Salomaa,V., Samani,N.J., Snieder,H., Sorensen,T.I.,
            Spector,T.D., Stefansson,K., Tonjes,A., Tuomilehto,J.,
            Uitterlinden,A.G., Uusitupa,M., van der Harst,P., Vollenweider,P.,
            Wallaschofski,H., Wareham,N.J., Watkins,H., Wichmann,H.E.,
            Wilson,J.F., Abecasis,G.R., Assimes,T.L., Barroso,I., Boehnke,M.,
            Borecki,I.B., Deloukas,P., Fox,C.S., Frayling,T., Groop,L.C.,
            Haritunian,T., Heid,I.M., Hunter,D., Kaplan,R.C., Karpe,F.,
            Moffatt,M.F., Mohlke,K.L., O'Connell,J.R., Pawitan,Y., Schadt,E.E.,
            Schlessinger,D., Steinthorsdottir,V., Strachan,D.P.,
            Thorsteinsdottir,U., van Duijn,C.M., Visscher,P.M., Di Blasio,A.M.,
            Hirschhorn,J.N., Lindgren,C.M., Morris,A.P., Meyre,D., Scherag,A.,
            McCarthy,M.I., Speliotes,E.K., North,K.E., Loos,R.J. and
            Ingelsson,E.
  TITLE     Genome-wide meta-analysis identifies 11 new loci for anthropometric
            traits and provides insights into genetic architecture
  JOURNAL   Nat Genet 45 (5), 501-512 (2013)
   PUBMED   23563607
REFERENCE   6  (bases 1 to 9574)
  AUTHORS   El Ghouzzi,V., Dagoneau,N., Kinning,E., Thauvin-Robinet,C.,
            Chemaitilly,W., Prost-Squarcioni,C., Al-Gazali,L.I., Verloes,A., Le
            Merrer,M., Munnich,A., Trembath,R.C. and Cormier-Daire,V.
  TITLE     Mutations in a novel gene Dymeclin (FLJ20071) are responsible for
            Dyggve-Melchior-Clausen syndrome
  JOURNAL   Hum Mol Genet 12 (3), 357-364 (2003)
   PUBMED   12554689
  REMARK    GeneRIF: Mutations cause Dyggve-Melchior-Clausen syndrome.  Normal
            function may be in process of intracellular digestion of proteins.
REFERENCE   7  (bases 1 to 9574)
  AUTHORS   Cohn,D.H., Ehtesham,N., Krakow,D., Unger,S., Shanske,A.,
            Reinker,K., Powell,B.R. and Rimoin,D.L.
  TITLE     Mental retardation and abnormal skeletal development
            (Dyggve-Melchior-Clausen dysplasia) due to mutations in a novel,
            evolutionarily conserved gene
  JOURNAL   Am J Hum Genet 72 (2), 419-428 (2003)
   PUBMED   12491225
  REMARK    GeneRIF: Gene mutations in a novel, evolutionarily conserved gene
            are identified in both rare autosomal recessive
            osteochondrodysplasias (DMC and SMC).
REFERENCE   8  (bases 1 to 9574)
  AUTHORS   Thauvin-Robinet,C., El Ghouzzi,V., Chemaitilly,W., Dagoneau,N.,
            Boute,O., Viot,G., Megarbane,A., Sefiani,A., Munnich,A., Le
            Merrer,M. and Cormier-Daire,V.
  TITLE     Homozygosity mapping of a Dyggve-Melchior-Clausen syndrome gene to
            chromosome 18q21.1
  JOURNAL   J Med Genet 39 (10), 714-717 (2002)
   PUBMED   12362026
REFERENCE   9  (bases 1 to 9574)
  AUTHORS   Ehtesham,N., Cantor,R.M., King,L.M., Reinker,K., Powell,B.R.,
            Shanske,A., Unger,S., Rimoin,D.L. and Cohn,D.H.
  TITLE     Evidence that Smith-McCort dysplasia and Dyggve-Melchior-Clausen
            dysplasia are allelic disorders that result from mutations in a
            gene on chromosome 18q12
  JOURNAL   Am J Hum Genet 71 (4), 947-951 (2002)
   PUBMED   12161821
REFERENCE   10 (bases 1 to 9574)
  AUTHORS   Jia,L., Young,M.F., Powell,J., Yang,L., Ho,N.C., Hotchkiss,R.,
            Robey,P.G. and Francomano,C.A.
  TITLE     Gene expression profile of human bone marrow stromal cells:
            high-throughput expressed sequence tag sequencing analysis
  JOURNAL   Genomics 79 (1), 7-17 (2002)
   PUBMED   11827452
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC100778.5, AC044840.10 and
            AC016866.12.
            
            Summary: This gene encodes a protein which regulates
            Golgi-associated secretory pathways that are essential to
            endochondral bone formation during early development. This gene is
            also believed to play a role in early brain development. This gene
            is widely expressed in embryos and is particularly abundant in
            chodrocytes and brain tissues. It encodes a peripheral membrane
            protein which shuttles between the cytosol and Golgi complex.
            Mutations in this gene are associated with two types of recessive
            osteochondrodysplasia: Dyggve-Melchior-Clausen (DMC) dysplasia and
            Smith-McCort (SMC) dysplasia. [provided by RefSeq, Jun 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.943494.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA2158569,
                                           SAMN03267768 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AC100778.5         39202-39449
            249-441             AC100778.5         69400-69592
            442-494             AC100778.5         108202-108254
            495-677             AC044840.10        3837-4019
            678-856             AC044840.10        8278-8456
            857-982             AC044840.10        18150-18275
            983-1096            AC044840.10        31960-32073
            1097-1191           AC044840.10        33349-33443
            1192-1356           AC044840.10        80738-80902
            1357-1459           AC044840.10        126666-126768
            1460-1642           AC016866.12        128603-128785       c
            1643-1756           AC016866.12        107261-107374       c
            1757-9574           AC016866.12        46246-54063         c
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     gene            1..9574
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="dymeclin"
                     /db_xref="GeneID:54808"
                     /db_xref="HGNC:HGNC:21317"
                     /db_xref="MIM:607461"
     exon            1..248
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            249..441
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..1906
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="isoform 21 is encoded by transcript variant 22;
                     dyggve-Melchior-Clausen syndrome protein"
                     /codon_start=1
                     /product="dymeclin isoform 21"
                     /protein_id="NP_001361370.1"
                     /db_xref="GeneID:54808"
                     /db_xref="HGNC:HGNC:21317"
                     /db_xref="MIM:607461"
                     /translation="MGSNSSRIGDLPKNEYLKKLSGTESISENDPFWNQLLSFSFPAP
                     TSSSELKLLEEATISVCRSLAGLWTVFTLGGVGSKAAASPELSSPLANQSLLLLLVLA
                     NLTDASDAPNPYRQAIMSFKNTQDSSPFPSSIPHAFQINFNSLYTALCEQQTSDQATL
                     LLYTLLHQNSNIRTYMLARTDMENLVLPILEILYHVEERNSHHVYMALIILLILTEDD
                     GFNRSIHEVILKNITWYSERVLTEISLGSLLILVVIRTIQYNMTRTRDKYLHTNCLAA
                     LANMSAQFRSLHQYAAQRIISYTCRHLRRYVYVLDKLYFPHSHCSTLQHCFSTLSDNG
                     EELLSLTCSHILRSYASSLFSLLSKKHNKVLEQATQSLRGSLSSNDVPLPDYAQDLNV
                     IEEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRTHPSFQDIMQNIDLVIS
                     FFSSRLLQAGAELSVERVLEIIKQGVVALPKDRLKKFPELKFKYVEEEQPEEFFIPYV
                     WSLVYNSAVGLYWNPQDIQLFTMDSD"
     exon            442..494
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            495..677
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            678..856
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            857..982
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1096
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1191
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1356
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1459
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1642
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1756
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..9574
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2134..2139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      2159
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="major polyA site"
     regulatory      4463..4468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      4487
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
     regulatory      7168..7173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      7194
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
     regulatory      9550..9555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: ATTAAA"
     polyA_site      9574
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
ORIGIN      
        1 ccctcttctc tcgccgcctc ctggcctccg caccgacgcg gcccgggctg gagccgagcc
       61 ggggccgagc tgcaggccgg accggagccg gatctgtacc cgctgagacg tggaaacatg
      121 gaggcctgag ccggtgtgcg ccacctgggc tgcggcggcg acagcgactt ctcctgaccc
      181 ctctgccacc ctcccatccg tccgcgggtc cgtggagctg gagcagatcc cccagccggc
      241 tgagacaggt tgtcttttgg aaatgcaggt ttaaggacaa attatctgct taagctagaa
      301 gatgggatcg aatagcagca gaatcggcga tcttcctaaa aatgagtact tgaaaaagtt
      361 atcaggcacg gaatctatct ctgagaatga cccgttctgg aatcagcttc tctcattttc
      421 tttccctgca ccaactagca gtagtgagtt gaaactcttg gaggaagcaa ccatttcagt
      481 ctgcaggtca ttagcaggac tctggactgt cttcacacta ggtggtgtgg gcagcaaagc
      541 ggctgcctct ccagagcttt cttcccctct ggccaaccag agtctcctgc ttctgctggt
      601 gttggccaat ctgacagatg cctcagatgc gccaaacccc tacagacaag ccattatgtc
      661 cttcaagaac acacaagata gcagtccttt cccctcatca attccacatg ccttccagat
      721 caactttaat agtttgtaca cagctctttg tgaacagcag acatctgatc aagcaactct
      781 cctcttgtat accttgctcc atcaaaatag taatattaga acatacatgt tggctcgcac
      841 agatatggaa aatcttgttt taccaattct tgagattctg tatcatgttg aagaaaggaa
      901 ttcacaccat gtgtatatgg cccttataat attgttgatc cttacggaag atgatggctt
      961 caacagatcc attcatgaag tgatactaaa aaatattact tggtattcag aacgagtttt
     1021 aactgaaatc tccttgggga gtctcctgat cctggtggta ataagaacca ttcaatacaa
     1081 catgactagg acacgagaca agtaccttca cacaaattgt ttggcagctt tagcaaatat
     1141 gtcggcacag tttcgttctc tccatcagta tgctgcccag aggatcatca gttatacctg
     1201 ccgccacttg cggagatatg tttatgtgtt ggacaaactg tatttccccc actctcactg
     1261 ttctacgctt cagcattgct tctcgaccct cagtgacaat ggagaggaac tcttgtcttt
     1321 aacttgctct cacattctca gatcctatgc ttccagttta ttttctttgc tgtctaaaaa
     1381 acacaacaaa gttctggaac aagccacaca gtccttgaga ggttcgctga gttctaatga
     1441 tgttcctcta ccagattatg cacaagacct aaatgtcatt gaagaagtga ttcgaatgat
     1501 gttagagatc atcaactcct gcctgacaaa ttcccttcac cacaacccaa acttggtata
     1561 cgccctgctt tacaaacgcg atctctttga acaatttcga actcatcctt catttcagga
     1621 tataatgcaa aatattgatc tggtgatctc cttctttagc tcaaggttgc tgcaagctgg
     1681 agctgagctg tcagtggaac gggtcctgga aatcattaag caaggcgtcg ttgcgctgcc
     1741 caaagacaga ctgaagaaat ttccagaatt gaaattcaaa tatgtggaag aggagcagcc
     1801 cgaggagttt tttatcccct atgtctggtc tcttgtctac aactcagcag tcggcctgta
     1861 ctggaatcca caggacatcc agctgttcac catggattcc gactgagggc aggatgctct
     1921 cccacccgga cccctccagc caagcagccc ttcaagttct tttatttctg ggtaacagaa
     1981 gtagacagac aggttacttg gtgtatcttc tgttaaagag gattgcacga gtgtgttttc
     2041 ctcacacact ttgatttgga gaattggtgc tagttggcaa tagataactc agcgtagata
     2101 gtattgcaaa aaggggagga aatacacaac aataataaat gtaaaaacct gctattcaac
     2161 atgcagtttt atttcgaagc caaaaatcta gagctttccc aagatcctgt tgccttaggc
     2221 acatcacact tcaacagtgc acactatcca acagtgcaca ctattcaaca gtgcacacta
     2281 ttcaaaagcg tagactattt ttttgcatgt tcaagatatt tgttttggtc ttatgtgtgt
     2341 gtgagagaga gagattcctt tgacattaag gagcatcaat gagaaaagat gatgaggcag
     2401 gaattaataa agaaatgaag tcgtgtgtgt ttggttgcct gtcagagggc acacaatttc
     2461 ataaacacca tgcctggaca atttgatatt aatatttaac acctctgcat cttttcttaa
     2521 aaaagaatat gggccagata cagtggctca catttgtaat cccagcactt tggggagcca
     2581 agttagcaga atcccttgag cacaggaatc tgaaaccagc ttgggcaaca tagtgagatc
     2641 ccatctctac aaaaaactta aaaattagcc aggcatgatg gcacattcct gtagtcctag
     2701 ctactcagga ggctaaggta ggaggattgc ctgagcccag gagttcaagg ctgcagtgag
     2761 ctaagaacgt gccagtacac tccagcctga gccacaaagt gagaccctgt ctcgcaaaaa
     2821 aaaaaaaaga aagaaaagaa aaagaaaatg gctacttctc atgaagccca gattaacttt
     2881 tctgtgaata tggcctgtga actgttgctg gaattaaatt ggagtctagc acaaattaag
     2941 ttaatctact ctgtattaat cattgggaaa aagaaaagct tcatttgaaa acagtctttt
     3001 tccttcaccc acactaatag aaaaaggaga gtaatttgtt catactgtat tccacgtggg
     3061 atgaaaagca tgttttgctc tttgtttctg ggccggtgtg atccgtgtgt tggtgcctga
     3121 gctggaggaa ggagcttctt gcagggaaac agccactggg gccacattga gggccagttg
     3181 ggaccttcct ttccagtcac actctgtgtc ctcacgggcc ccttcacagt ctagataagg
     3241 agcctagttt cattctcaaa gaaaaagtat ttggtcttca acatagaaag cacaaaagcc
     3301 cagacgagac tgagcacact gttcgaatcc actgccagct catctgtgtc agggctggct
     3361 tctcctgtcc tgtgtcccag ggctgcaggc ccgtgttatt tgagtccatc aaatcttccg
     3421 agggcctcgg aggaaccatt ctcagtcacc ccaagctctg taaaaggtca aaacactggc
     3481 agcctctcag tgcaccactg tgtgggctcc acacgggaag gcctggccaa gctgtgctct
     3541 tagaggctgt cgaggtaggg caggaagctt ccctatcctc gtctggatgg aatgaaacag
     3601 aggttcccta aggccaaggg actcgtggag cgcccatccc gggtggggtc ccagaggtga
     3661 gtgcagcttg cctcctgtcc ccaaggggct tgttctgtca ggcctcctcc agtcactgct
     3721 ggagtgggga ctctgactcc tgtgttcacc tcagagccca tgtacaggct agaggtgagg
     3781 cttggcaaca acttctacta atacttagct ttgtggcaga gccagtctga aggaggagaa
     3841 gcgctctcag ccagcagggg gagtgatgat tatcatttca ggtagcaagg atatgtattt
     3901 actatgtggg gaaataattt gcaactttaa tttgtttcca aagcattttg attggcttcc
     3961 ttttccttta aaccaaaaaa ttgtttaaat gtttaagggt catgtggaac agctgagctt
     4021 ctggcaggac cacagaactg tcctctgagt ctgagggctg ggcagtgcca caggacagtc
     4081 cctgggctgc aggactgtca gtgcttgttg tgtgaggaaa tgaggccctt caaaaaccca
     4141 gtcatcttga cagagggcac actcccaagg aaggcctgac tggggaatca tacagcctct
     4201 cctccaggcc tctgggctca ctgaggtgcc cccagaaccc tgattgccct cccctctttc
     4261 ccttttctca ttcatcacac agtagaagca ctcactagct aacagctcag ctggggcagc
     4321 agcctccatg gaggcctgca agtgatgctc ccagtcctgg ttcccgctca ggagggaggc
     4381 cccaccacgt gcatcactgc ctcagctctg caaagtcacc agaggcttct tcctgatttt
     4441 tgtgaaagat ttattatatg gcaataaatc tgaatgataa actttcatta tacttgcatt
     4501 aattttgagt attagtttgc ttagaaattt ttacacgaag taaaaatgtg taatgatgca
     4561 acacaggatc atcgtgtgtt cagatggaaa ttggcatctg attttacccc agatctgaag
     4621 ctgttactgc attgtgtcat tttatcaaca aagacttctg ccacaatatt tacttttaaa
     4681 agaatttgtc cctttcaata gcatcttccc tatcaagagt tcaaggcact ttgcagaaga
     4741 gcttcagatt tctaactcta gttcccacag tcacccgttg aggctggtgt catacaatct
     4801 atgaacaaca accttgcagc aggactgcca ccattcagca gtgcagacaa cacagctcaa
     4861 ggacaaagac agagaaagga aagaagagaa ggtccctgaa ggtgaagcat agaaagtaca
     4921 ctgcacacac agttctctct ggaaggaaag cacaggagct gggaagtaga cgatagaaag
     4981 tggaaaggac acaggaccag catcagaaca ctcagcaact tcctaagagc ttgctaaggt
     5041 acatccaaga gaaatattta gtgagggaaa cttttaagca ctggaattgt taatatatca
     5101 tactttaagc aacatttcgt cctttagatt ccgggagcca gggttttgca ttgggtggta
     5161 gagctctctg cataattgag tcaccctgtg cttttttgca gagccataaa tttttggcac
     5221 ttgctgatac ttcataggct ggagtctctg caaggtcaac cctttgggtc tcttaattct
     5281 ttcctaggca ctatcctgca atatcagtag ttgccacttg attttaggga ctgattatta
     5341 ggccttctga ctccaaattc agaacaattc cagtgtccta catcacctcc tggagtcaaa
     5401 gaggtcagtt tctattcagt gatgaagagc taagctagct atgcatatga agaactgaga
     5461 agtagattat aacacacaat ccacagcaaa taatgcatat ttattttgta tgaattaaag
     5521 cataaaattt aatctcatta gcgtatagct attcagagtt cccactcaaa gcaagggaga
     5581 ctcaccagta gccccaagac ccttggccag gtcatactca acccctaatc taacttcaca
     5641 aggctgtcta aatgtcagct actcagacac agcctctggc attagcaaaa tcccccagag
     5701 gaaaagcagc cccatagaac tggacaagta atacttaact atcttgttga ctctggggtc
     5761 cttcaagcaa attttaaaaa tatttttacc agatttccaa gttgtcctta gcttgagggt
     5821 tggtcctcca ttactgtcat tggactatcc ctcatgggta gtgcagttca acttctaaac
     5881 cacctcagta cctgaatgtg gtccgaggtg aaaccagatt actgatgcct tcaggtgccc
     5941 aattgaagca aatggcaaat tctgtggagg aagtaaacat aacccaaggc ctcaaattat
     6001 ttctgcagtg ttgcaatcag aaatacccag gtatgggaga agaccagaca acattaatta
     6061 cttgaattaa aaccattaga ggaaaacaca gacaatagaa atatccaaac agactccaga
     6121 taaagttgtc gcagaattta aaacagctat ggttactgtg ttcaaaaaaa tgagacgcat
     6181 ggtcaagaat ttcaacagag agttagaaac tgtaaaaggc gcattggaaa ttctataact
     6241 aaagtactgt aatgagaaga acagtgaaca ggtttaagag cagattagaa ataactgaaa
     6301 agagaatgag taagatgaaa aattgagggt gaggggtggg agcaggaatt atatagtaaa
     6361 aaaaacagaa catggagaat agagggtaag agacttaaag ggtacagtga gcatgtctga
     6421 cctgctcaga catgcagggt agttggcatc tcaaaagagt agaagaaagg aatatttaaa
     6481 caaatggctg agaacttttc caaaactcat gaaagatatc acaccaaaaa ttcaagaatc
     6541 cctcccacaa actccaagtg ggataaataa aaacaaccca cacctagaaa catcatagta
     6601 aaactgttga agaccaaaga aaaataatat tttagacctg ctggggtggg agagggaata
     6661 taaccattaa aggagcaata atattagact gacagctcct tctcagcaga aacaatggaa
     6721 gacagtgaaa tactgccttt tatggttctg aaagaaaatg actgccaacc taaaaatttc
     6781 taatcaggga aaatatcctg caaaaatgaa gaggatatga agacattttt agagaaacag
     6841 ctagaagaat taatcagcac gcctacccta aaggaaaatt taaaaggtgt tttacaggca
     6901 gaaggaaaat aatcccaaac gtaaggacag agccctagga aagattgaag agcaatgaaa
     6961 atgacacata aatattgact acataaaaca ataattatgt cttatggagt tttaaatata
     7021 tagagaaata aaatgtatga aaatgaaagg atttatgtgg gaattttaag tgttctaaga
     7081 cacttggcat catccaagaa gagagtaaaa attcatttaa tattagtctt tgataactca
     7141 tgtatgcatg ataatatttg agatatcaat aaaagtgtat agctttccac ataataagag
     7201 agaattcgaa taatcaattc agtggaagat ttaagaattg caaaagagtc taagaaagaa
     7261 gaaaaagcag aacaggtgga acaaatagaa agcaagatat ttgtgataaa tccattatta
     7321 cactaaatgt aaatggacta atgctcaaat gttgacaaat attgtcagcc ttgttttaaa
     7381 ccatatgcta tttacaagag acatttttag aacatgaatt tagaaatttt ggaagaaaaa
     7441 gatggagaaa tacataccca gcaaacagta accatgagga aactggtgta gatatattta
     7501 agtaaacttt aaggcaataa tttttttaag aaataaaagg gtcaattcaa ttactagaaa
     7561 gataatctaa atttatatgc acttaaaata taaactcaaa atatgtaaaa gaaaatttga
     7621 taggaaattt agacaaatat gcaaccagag tgatatttta acatgcctat tttgttaatt
     7681 ggtagaaaaa ctagcataca aaaatcaagt tataaaagat ttaagcaatg aaatgagcaa
     7741 accaaacgga tgtatgtagg acaccaaact caataacata cttttcaaac acaaatggca
     7801 cacactccaa aatactgggc tataaaggtt tcaacaggct aggcacagtg gctcacatct
     7861 gtaatactag cactttgaga ggccaagtca ggaagatcac ttgagcccag gagtttgaga
     7921 ccagcttggg caacataatg aggccccacc tttacaaaaa ataaaaaaat taactgggca
     7981 tggtggcatg tgcctatagc cccagctact tgggaagctg aggcaggaga atcacttgtg
     8041 cccaggaggt cgaggctgca gtgagctata attgcactac tgcactccag cttgggtgac
     8101 agagcaagac cctgtctcag aaaaaaagtc tcaacaaacc tgaaaatact tcaaatgaaa
     8161 tatgttctct gaccataata gaatcaagac taattattta caaaaagata attttataat
     8221 cctcatattt ttggaaatag atttccaggt aacccatctg tcagaatctt aatggaaatt
     8281 tgaaatattt tgaactgatc atgaaaatac tacatatcaa aacagagaaa aaacttgtgg
     8341 gatgcaaata aaactatgct tagaagacct tttaatttta ttttaaattt aaagagtacc
     8401 tgttcaggtt tgttacaaga atatattgat gatgctgagg tttgggctcc taatgatcct
     8461 gtcacccaga tagtgaacat agtacccaat agaaagtttt tcagcacttg cttccctctc
     8521 tccttccctt cctccctcgt tttggagtcc ctagtgtcta ttgttcatat atatatatac
     8581 acacacatat atatttaaat gtttatgtta gaaaagaaga aaggttaaaa atcaattaag
     8641 caattacctc aaaaatttta aaaacagcac atgaaaccca aaaataagcc aaattactga
     8701 tatagaacac aaatatacaa taaagggaat caaagtcaaa agttggttat ttaaaaggat
     8761 taagaggact cattggctgg gcatgttggc tcacatgtgt aatcccagca ctttgggagt
     8821 cacctgaggt caggagttca agaccagcct ggccaacatg atgaaacccc gtctctacta
     8881 aaaatacaaa aattagctgg gcgtggtggt gggcatctgt agtcccagct acgcaggagg
     8941 ctgaggcaag agaattgctt gaacctggga ggtggagggt tcagtgggca gagattgtgc
     9001 cactgcactc cagcctgggc aacagagcga gactccgtcc caaaataaat aaataaaaga
     9061 attcataacc ccttaaaaga ctgatcaaag aaggaaggag aaagggcagg agagaggaat
     9121 agccaatatc agaaaggaaa acaggtaagt tacttcagat tatgcagaca tttaaaatat
     9181 ttaagagcca gcctggataa catggtgaaa ccctcctgtc tctacacaca cacacacaca
     9241 aattagctga gcatggtggt gtgtgcctgt agtcccagct actggagagg ctaaggtagg
     9301 aggattacct gagcccagga ggtagaggct gcagtgagct gtggtcatgg caccacactc
     9361 cagcctgggc aatggagtga gagaccgtgt ctcaaaaata tgtatacata tttaagagaa
     9421 tattatgaat accaataaat tttaaatcta gttgaaatga tctttattgc agtgataggt
     9481 gtatacatat gtctaatcaa attgtacact ttattacata tgtgcagttt tatatattaa
     9541 gtatgcctca ttaaagttgt ttttagattt tgca
//
