LOCUS       NM_001353214           10144 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens dymeclin (DYM), transcript variant 6, mRNA.
ACCESSION   NM_001353214
VERSION     NM_001353214.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Abdullah, Shah,P.W., Nawaz,S., Hussain,S., Ullah,A., Basit,S. and
            Ahmad,W.
  TITLE     A homozygous nonsense variant in DYM underlies
            Dyggve-Melchior-Clausen syndrome associated with ectodermal
            features
  JOURNAL   Mol Biol Rep 47 (9), 7083-7088 (2020)
   PUBMED   32886330
  REMARK    GeneRIF: A homozygous nonsense variant in DYM underlies
            Dyggve-Melchior-Clausen syndrome associated with ectodermal
            features.
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Dupuis,N., Fafouri,A., Bayot,A., Kumar,M., Lecharpentier,T.,
            Ball,G., Edwards,D., Bernard,V., Dournaud,P., Drunat,S.,
            Vermelle-Andrzejewski,M., Vilain,C., Abramowicz,M., Desir,J.,
            Bonaventure,J., Gareil,N., Boncompain,G., Csaba,Z., Perez,F.,
            Passemard,S., Gressens,P. and El Ghouzzi,V.
  TITLE     Dymeclin deficiency causes postnatal microcephaly, hypomyelination
            and reticulum-to-Golgi trafficking defects in mice and humans
  JOURNAL   Hum Mol Genet 24 (10), 2771-2783 (2015)
   PUBMED   25652408
  REMARK    GeneRIF: Dymeclin is crucial for proper myelination and anterograde
            neuronal trafficking, two processes that are highly active during
            postnatal brain maturation.
REFERENCE   3  (bases 1 to 10144)
  AUTHORS   He,M., Xu,M., Zhang,B., Liang,J., Chen,P., Lee,J.Y., Johnson,T.A.,
            Li,H., Yang,X., Dai,J., Liang,L., Gui,L., Qi,Q., Huang,J., Li,Y.,
            Adair,L.S., Aung,T., Cai,Q., Cheng,C.Y., Cho,M.C., Cho,Y.S.,
            Chu,M., Cui,B., Gao,Y.T., Go,M.J., Gu,D., Gu,W., Guo,H., Hao,Y.,
            Hong,J., Hu,Z., Hu,Y., Huang,J., Hwang,J.Y., Ikram,M.K., Jin,G.,
            Kang,D.H., Khor,C.C., Kim,B.J., Kim,H.T., Kubo,M., Lee,J., Lee,J.,
            Lee,N.R., Li,R., Li,J., Liu,J., Longe,J., Lu,W., Lu,X., Miao,X.,
            Okada,Y., Ong,R.T., Qiu,G., Seielstad,M., Sim,X., Song,H.,
            Takeuchi,F., Tanaka,T., Taylor,P.R., Wang,L., Wang,W., Wang,Y.,
            Wu,C., Wu,Y., Xiang,Y.B., Yamamoto,K., Yang,H., Liao,M., Yokota,M.,
            Young,T., Zhang,X., Kato,N., Wang,Q.K., Zheng,W., Hu,F.B., Lin,D.,
            Shen,H., Teo,Y.Y., Mo,Z., Wong,T.Y., Lin,X., Mohlke,K.L., Ning,G.,
            Tsunoda,T., Han,B.G., Shu,X.O., Tai,E.S., Wu,T. and Qi,L.
  TITLE     Meta-analysis of genome-wide association studies of adult height in
            East Asians identifies 17 novel loci
  JOURNAL   Hum Mol Genet 24 (6), 1791-1800 (2015)
   PUBMED   25429064
REFERENCE   4  (bases 1 to 10144)
  AUTHORS   Seven,M., Koparir,E., Gezdirici,A., Aydin,H., Skladny,H.,
            Fenercioglu,E., Guven,G., Karatas,O.F., Koparir,A., Ozen,M. and
            Ulucan,H.
  TITLE     A novel frameshift mutation and infrequent clinical findings in two
            cases with Dyggve-Melchior-Clausen syndrome
  JOURNAL   Clin Dysmorphol 23 (1), 1-7 (2014)
   PUBMED   24300288
  REMARK    GeneRIF: The clinical diagnosis was confirmed with molecular
            analysis of DYM with a known mutation at c.580C>T (p.R194X).
REFERENCE   5  (bases 1 to 10144)
  AUTHORS   Berndt,S.I., Gustafsson,S., Magi,R., Ganna,A., Wheeler,E.,
            Feitosa,M.F., Justice,A.E., Monda,K.L., Croteau-Chonka,D.C.,
            Day,F.R., Esko,T., Fall,T., Ferreira,T., Gentilini,D.,
            Jackson,A.U., Luan,J., Randall,J.C., Vedantam,S., Willer,C.J.,
            Winkler,T.W., Wood,A.R., Workalemahu,T., Hu,Y.J., Lee,S.H.,
            Liang,L., Lin,D.Y., Min,J.L., Neale,B.M., Thorleifsson,G., Yang,J.,
            Albrecht,E., Amin,N., Bragg-Gresham,J.L., Cadby,G., den Heijer,M.,
            Eklund,N., Fischer,K., Goel,A., Hottenga,J.J., Huffman,J.E.,
            Jarick,I., Johansson,A., Johnson,T., Kanoni,S., Kleber,M.E.,
            Konig,I.R., Kristiansson,K., Kutalik,Z., Lamina,C., Lecoeur,C.,
            Li,G., Mangino,M., McArdle,W.L., Medina-Gomez,C.,
            Muller-Nurasyid,M., Ngwa,J.S., Nolte,I.M., Paternoster,L.,
            Pechlivanis,S., Perola,M., Peters,M.J., Preuss,M., Rose,L.M.,
            Shi,J., Shungin,D., Smith,A.V., Strawbridge,R.J., Surakka,I.,
            Teumer,A., Trip,M.D., Tyrer,J., Van Vliet-Ostaptchouk,J.V.,
            Vandenput,L., Waite,L.L., Zhao,J.H., Absher,D., Asselbergs,F.W.,
            Atalay,M., Attwood,A.P., Balmforth,A.J., Basart,H., Beilby,J.,
            Bonnycastle,L.L., Brambilla,P., Bruinenberg,M., Campbell,H.,
            Chasman,D.I., Chines,P.S., Collins,F.S., Connell,J.M.,
            Cookson,W.O., de Faire,U., de Vegt,F., Dei,M., Dimitriou,M.,
            Edkins,S., Estrada,K., Evans,D.M., Farrall,M., Ferrario,M.M.,
            Ferrieres,J., Franke,L., Frau,F., Gejman,P.V., Grallert,H.,
            Gronberg,H., Gudnason,V., Hall,A.S., Hall,P., Hartikainen,A.L.,
            Hayward,C., Heard-Costa,N.L., Heath,A.C., Hebebrand,J., Homuth,G.,
            Hu,F.B., Hunt,S.E., Hypponen,E., Iribarren,C., Jacobs,K.B.,
            Jansson,J.O., Jula,A., Kahonen,M., Kathiresan,S., Kee,F.,
            Khaw,K.T., Kivimaki,M., Koenig,W., Kraja,A.T., Kumari,M.,
            Kuulasmaa,K., Kuusisto,J., Laitinen,J.H., Lakka,T.A.,
            Langenberg,C., Launer,L.J., Lind,L., Lindstrom,J., Liu,J.,
            Liuzzi,A., Lokki,M.L., Lorentzon,M., Madden,P.A., Magnusson,P.K.,
            Manunta,P., Marek,D., Marz,W., Mateo Leach,I., McKnight,B.,
            Medland,S.E., Mihailov,E., Milani,L., Montgomery,G.W., Mooser,V.,
            Muhleisen,T.W., Munroe,P.B., Musk,A.W., Narisu,N., Navis,G.,
            Nicholson,G., Nohr,E.A., Ong,K.K., Oostra,B.A., Palmer,C.N.,
            Palotie,A., Peden,J.F., Pedersen,N., Peters,A., Polasek,O.,
            Pouta,A., Pramstaller,P.P., Prokopenko,I., Putter,C.,
            Radhakrishnan,A., Raitakari,O., Rendon,A., Rivadeneira,F.,
            Rudan,I., Saaristo,T.E., Sambrook,J.G., Sanders,A.R., Sanna,S.,
            Saramies,J., Schipf,S., Schreiber,S., Schunkert,H., Shin,S.Y.,
            Signorini,S., Sinisalo,J., Skrobek,B., Soranzo,N., Stancakova,A.,
            Stark,K., Stephens,J.C., Stirrups,K., Stolk,R.P., Stumvoll,M.,
            Swift,A.J., Theodoraki,E.V., Thorand,B., Tregouet,D.A., Tremoli,E.,
            Van der Klauw,M.M., van Meurs,J.B., Vermeulen,S.H., Viikari,J.,
            Virtamo,J., Vitart,V., Waeber,G., Wang,Z., Widen,E., Wild,S.H.,
            Willemsen,G., Winkelmann,B.R., Witteman,J.C., Wolffenbuttel,B.H.,
            Wong,A., Wright,A.F., Zillikens,M.C., Amouyel,P., Boehm,B.O.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Chanock,S.J.,
            Cupples,L.A., Cusi,D., Dedoussis,G.V., Erdmann,J., Eriksson,J.G.,
            Franks,P.W., Froguel,P., Gieger,C., Gyllensten,U., Hamsten,A.,
            Harris,T.B., Hengstenberg,C., Hicks,A.A., Hingorani,A., Hinney,A.,
            Hofman,A., Hovingh,K.G., Hveem,K., Illig,T., Jarvelin,M.R.,
            Jockel,K.H., Keinanen-Kiukaanniemi,S.M., Kiemeney,L.A., Kuh,D.,
            Laakso,M., Lehtimaki,T., Levinson,D.F., Martin,N.G., Metspalu,A.,
            Morris,A.D., Nieminen,M.S., Njolstad,I., Ohlsson,C.,
            Oldehinkel,A.J., Ouwehand,W.H., Palmer,L.J., Penninx,B., Power,C.,
            Province,M.A., Psaty,B.M., Qi,L., Rauramaa,R., Ridker,P.M.,
            Ripatti,S., Salomaa,V., Samani,N.J., Snieder,H., Sorensen,T.I.,
            Spector,T.D., Stefansson,K., Tonjes,A., Tuomilehto,J.,
            Uitterlinden,A.G., Uusitupa,M., van der Harst,P., Vollenweider,P.,
            Wallaschofski,H., Wareham,N.J., Watkins,H., Wichmann,H.E.,
            Wilson,J.F., Abecasis,G.R., Assimes,T.L., Barroso,I., Boehnke,M.,
            Borecki,I.B., Deloukas,P., Fox,C.S., Frayling,T., Groop,L.C.,
            Haritunian,T., Heid,I.M., Hunter,D., Kaplan,R.C., Karpe,F.,
            Moffatt,M.F., Mohlke,K.L., O'Connell,J.R., Pawitan,Y., Schadt,E.E.,
            Schlessinger,D., Steinthorsdottir,V., Strachan,D.P.,
            Thorsteinsdottir,U., van Duijn,C.M., Visscher,P.M., Di Blasio,A.M.,
            Hirschhorn,J.N., Lindgren,C.M., Morris,A.P., Meyre,D., Scherag,A.,
            McCarthy,M.I., Speliotes,E.K., North,K.E., Loos,R.J. and
            Ingelsson,E.
  TITLE     Genome-wide meta-analysis identifies 11 new loci for anthropometric
            traits and provides insights into genetic architecture
  JOURNAL   Nat Genet 45 (5), 501-512 (2013)
   PUBMED   23563607
REFERENCE   6  (bases 1 to 10144)
  AUTHORS   El Ghouzzi,V., Dagoneau,N., Kinning,E., Thauvin-Robinet,C.,
            Chemaitilly,W., Prost-Squarcioni,C., Al-Gazali,L.I., Verloes,A., Le
            Merrer,M., Munnich,A., Trembath,R.C. and Cormier-Daire,V.
  TITLE     Mutations in a novel gene Dymeclin (FLJ20071) are responsible for
            Dyggve-Melchior-Clausen syndrome
  JOURNAL   Hum Mol Genet 12 (3), 357-364 (2003)
   PUBMED   12554689
  REMARK    GeneRIF: Mutations cause Dyggve-Melchior-Clausen syndrome.  Normal
            function may be in process of intracellular digestion of proteins.
REFERENCE   7  (bases 1 to 10144)
  AUTHORS   Cohn,D.H., Ehtesham,N., Krakow,D., Unger,S., Shanske,A.,
            Reinker,K., Powell,B.R. and Rimoin,D.L.
  TITLE     Mental retardation and abnormal skeletal development
            (Dyggve-Melchior-Clausen dysplasia) due to mutations in a novel,
            evolutionarily conserved gene
  JOURNAL   Am J Hum Genet 72 (2), 419-428 (2003)
   PUBMED   12491225
  REMARK    GeneRIF: Gene mutations in a novel, evolutionarily conserved gene
            are identified in both rare autosomal recessive
            osteochondrodysplasias (DMC and SMC).
REFERENCE   8  (bases 1 to 10144)
  AUTHORS   Thauvin-Robinet,C., El Ghouzzi,V., Chemaitilly,W., Dagoneau,N.,
            Boute,O., Viot,G., Megarbane,A., Sefiani,A., Munnich,A., Le
            Merrer,M. and Cormier-Daire,V.
  TITLE     Homozygosity mapping of a Dyggve-Melchior-Clausen syndrome gene to
            chromosome 18q21.1
  JOURNAL   J Med Genet 39 (10), 714-717 (2002)
   PUBMED   12362026
REFERENCE   9  (bases 1 to 10144)
  AUTHORS   Ehtesham,N., Cantor,R.M., King,L.M., Reinker,K., Powell,B.R.,
            Shanske,A., Unger,S., Rimoin,D.L. and Cohn,D.H.
  TITLE     Evidence that Smith-McCort dysplasia and Dyggve-Melchior-Clausen
            dysplasia are allelic disorders that result from mutations in a
            gene on chromosome 18q12
  JOURNAL   Am J Hum Genet 71 (4), 947-951 (2002)
   PUBMED   12161821
REFERENCE   10 (bases 1 to 10144)
  AUTHORS   Jia,L., Young,M.F., Powell,J., Yang,L., Ho,N.C., Hotchkiss,R.,
            Robey,P.G. and Francomano,C.A.
  TITLE     Gene expression profile of human bone marrow stromal cells:
            high-throughput expressed sequence tag sequencing analysis
  JOURNAL   Genomics 79 (1), 7-17 (2002)
   PUBMED   11827452
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC100778.5, AC090329.7,
            AC044840.10 and AC016866.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 28, 2019 this sequence version replaced NM_001353214.2.
            
            Summary: This gene encodes a protein which regulates
            Golgi-associated secretory pathways that are essential to
            endochondral bone formation during early development. This gene is
            also believed to play a role in early brain development. This gene
            is widely expressed in embryos and is particularly abundant in
            chodrocytes and brain tissues. It encodes a peripheral membrane
            protein which shuttles between the cytosol and Golgi complex.
            Mutations in this gene are associated with two types of recessive
            osteochondrodysplasia: Dyggve-Melchior-Clausen (DMC) dysplasia and
            Smith-McCort (SMC) dysplasia. [provided by RefSeq, Jun 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.174055.1,
                                           SRR1660809.89956.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA2158569,
                                           SAMN03267768 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000675505.1/ ENSP00000501694.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AC100778.5         39202-39449
            249-441             AC100778.5         69400-69592
            442-494             AC100778.5         108202-108254
            495-588             AC100778.5         120089-120182
            589-722             AC100778.5         121147-121280
            723-795             AC100778.5         136614-136686
            796-921             AC090329.7         10948-11073
            922-1064            AC090329.7         12795-12937
            1065-1247           AC044840.10        3837-4019
            1248-1426           AC044840.10        8278-8456
            1427-1552           AC044840.10        18150-18275
            1553-1666           AC044840.10        31960-32073
            1667-1761           AC044840.10        33349-33443
            1762-1926           AC044840.10        80738-80902
            1927-2029           AC044840.10        126666-126768
            2030-2212           AC016866.12        128603-128785       c
            2213-2326           AC016866.12        107261-107374       c
            2327-10144          AC016866.12        46246-54063         c
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     gene            1..10144
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="dymeclin"
                     /db_xref="GeneID:54808"
                     /db_xref="HGNC:HGNC:21317"
                     /db_xref="MIM:607461"
     exon            1..248
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            249..441
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..2476
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="isoform 6 is encoded by transcript variant 6;
                     dyggve-Melchior-Clausen syndrome protein"
                     /codon_start=1
                     /product="dymeclin isoform 6"
                     /protein_id="NP_001340143.1"
                     /db_xref="CCDS:CCDS92460.1"
                     /db_xref="GeneID:54808"
                     /db_xref="HGNC:HGNC:21317"
                     /db_xref="MIM:607461"
                     /translation="MGSNSSRIGDLPKNEYLKKLSGTESISENDPFWNQLLSFSFPAP
                     TSSSELKLLEEATISVCRSLVENNPRTGNLGALIKVFLSRTKELKLSAECQNHIFIWQ
                     THNALFIICCLLKVFICQMSEEELQLHFTYEEKSPGNYSSDSEDLLEELLCCLMQLIT
                     DIPLLDITYEISVEAISTMVVFLSCQLFHKEVLRQSISHKYLMRGPCLPYTSKLVKTL
                     LYNFIRQEKPPPPGAHVFPQQSDGGGLLYGLASGVATGLWTVFTLGGVGSKAAASPEL
                     SSPLANQSLLLLLVLANLTDASDAPNPYRQAIMSFKNTQDSSPFPSSIPHAFQINFNS
                     LYTALCEQQTSDQATLLLYTLLHQNSNIRTYMLARTDMENLVLPILEILYHVEERNSH
                     HVYMALIILLILTEDDGFNRSIHEVILKNITWYSERVLTEISLGSLLILVVIRTIQYN
                     MTRTRDKYLHTNCLAALANMSAQFRSLHQYAAQRIISYTCRHLRRYVYVLDKLYFPHS
                     HCSTLQHCFSTLSDNGEELLSLTCSHILRSYASSLFSLLSKKHNKVLEQATQSLRGSL
                     SSNDVPLPDYAQDLNVIEEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRT
                     HPSFQDIMQNIDLVISFFSSRLLQAGAELSVERVLEIIKQGVVALPKDRLKKFPELKF
                     KYVEEEQPEEFFIPYVWSLVYNSAVGLYWNPQDIQLFTMDSD"
     exon            442..494
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            495..588
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            589..722
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            723..795
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            796..921
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1064
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1247
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1426
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1552
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1666
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1761
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1926
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2029
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2212
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2326
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..10144
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2704..2709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      2729
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="major polyA site"
     regulatory      5033..5038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      5057
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
     regulatory      7738..7743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: AATAAA"
     polyA_site      7764
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
     regulatory      10120..10125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
                     /note="hexamer: ATTAAA"
     polyA_site      10144
                     /gene="DYM"
                     /gene_synonym="DMC; SMC"
ORIGIN      
        1 ccctcttctc tcgccgcctc ctggcctccg caccgacgcg gcccgggctg gagccgagcc
       61 ggggccgagc tgcaggccgg accggagccg gatctgtacc cgctgagacg tggaaacatg
      121 gaggcctgag ccggtgtgcg ccacctgggc tgcggcggcg acagcgactt ctcctgaccc
      181 ctctgccacc ctcccatccg tccgcgggtc cgtggagctg gagcagatcc cccagccggc
      241 tgagacaggt tgtcttttgg aaatgcaggt ttaaggacaa attatctgct taagctagaa
      301 gatgggatcg aatagcagca gaatcggcga tcttcctaaa aatgagtact tgaaaaagtt
      361 atcaggcacg gaatctatct ctgagaatga cccgttctgg aatcagcttc tctcattttc
      421 tttccctgca ccaactagca gtagtgagtt gaaactcttg gaggaagcaa ccatttcagt
      481 ctgcaggtca ttagttgaaa acaatcctcg aacaggaaat cttggtgcac taattaaggt
      541 cttcctttct agaaccaaag aactaaaact ttcagcagaa tgtcagaacc acatcttcat
      601 ttggcagaca cacaatgctt tgtttattat ttgctgtttg ctgaaagtgt tcatctgtca
      661 gatgtcagag gaggaattac aacttcattt tacttatgaa gaaaaatctc ctggcaatta
      721 cagttctgac tcagaagatc ttttggaaga attgctgtgc tgtttgatgc agttgatcac
      781 tgatattcca ctcttagata ttacatatga aatatcagta gaagctatat caacaatggt
      841 tgttttcctt tcctgccaac tcttccacaa agaagttttg cgacagagca tcagccacaa
      901 gtatttgatg cgaggtccat gtcttccata caccagcaaa cttgtgaaga ccttattata
      961 taactttatc agacaagaaa agccacctcc tccaggggcc catgttttcc ctcagcagtc
     1021 ggatggggga ggactgcttt atggacttgc atcaggagta gcaacaggac tctggactgt
     1081 cttcacacta ggtggtgtgg gcagcaaagc ggctgcctct ccagagcttt cttcccctct
     1141 ggccaaccag agtctcctgc ttctgctggt gttggccaat ctgacagatg cctcagatgc
     1201 gccaaacccc tacagacaag ccattatgtc cttcaagaac acacaagata gcagtccttt
     1261 cccctcatca attccacatg ccttccagat caactttaat agtttgtaca cagctctttg
     1321 tgaacagcag acatctgatc aagcaactct cctcttgtat accttgctcc atcaaaatag
     1381 taatattaga acatacatgt tggctcgcac agatatggaa aatcttgttt taccaattct
     1441 tgagattctg tatcatgttg aagaaaggaa ttcacaccat gtgtatatgg cccttataat
     1501 attgttgatc cttacggaag atgatggctt caacagatcc attcatgaag tgatactaaa
     1561 aaatattact tggtattcag aacgagtttt aactgaaatc tccttgggga gtctcctgat
     1621 cctggtggta ataagaacca ttcaatacaa catgactagg acacgagaca agtaccttca
     1681 cacaaattgt ttggcagctt tagcaaatat gtcggcacag tttcgttctc tccatcagta
     1741 tgctgcccag aggatcatca gttatacctg ccgccacttg cggagatatg tttatgtgtt
     1801 ggacaaactg tatttccccc actctcactg ttctacgctt cagcattgct tctcgaccct
     1861 cagtgacaat ggagaggaac tcttgtcttt aacttgctct cacattctca gatcctatgc
     1921 ttccagttta ttttctttgc tgtctaaaaa acacaacaaa gttctggaac aagccacaca
     1981 gtccttgaga ggttcgctga gttctaatga tgttcctcta ccagattatg cacaagacct
     2041 aaatgtcatt gaagaagtga ttcgaatgat gttagagatc atcaactcct gcctgacaaa
     2101 ttcccttcac cacaacccaa acttggtata cgccctgctt tacaaacgcg atctctttga
     2161 acaatttcga actcatcctt catttcagga tataatgcaa aatattgatc tggtgatctc
     2221 cttctttagc tcaaggttgc tgcaagctgg agctgagctg tcagtggaac gggtcctgga
     2281 aatcattaag caaggcgtcg ttgcgctgcc caaagacaga ctgaagaaat ttccagaatt
     2341 gaaattcaaa tatgtggaag aggagcagcc cgaggagttt tttatcccct atgtctggtc
     2401 tcttgtctac aactcagcag tcggcctgta ctggaatcca caggacatcc agctgttcac
     2461 catggattcc gactgagggc aggatgctct cccacccgga cccctccagc caagcagccc
     2521 ttcaagttct tttatttctg ggtaacagaa gtagacagac aggttacttg gtgtatcttc
     2581 tgttaaagag gattgcacga gtgtgttttc ctcacacact ttgatttgga gaattggtgc
     2641 tagttggcaa tagataactc agcgtagata gtattgcaaa aaggggagga aatacacaac
     2701 aataataaat gtaaaaacct gctattcaac atgcagtttt atttcgaagc caaaaatcta
     2761 gagctttccc aagatcctgt tgccttaggc acatcacact tcaacagtgc acactatcca
     2821 acagtgcaca ctattcaaca gtgcacacta ttcaaaagcg tagactattt ttttgcatgt
     2881 tcaagatatt tgttttggtc ttatgtgtgt gtgagagaga gagattcctt tgacattaag
     2941 gagcatcaat gagaaaagat gatgaggcag gaattaataa agaaatgaag tcgtgtgtgt
     3001 ttggttgcct gtcagagggc acacaatttc ataaacacca tgcctggaca atttgatatt
     3061 aatatttaac acctctgcat cttttcttaa aaaagaatat gggccagata cagtggctca
     3121 catttgtaat cccagcactt tggggagcca agttagcaga atcccttgag cacaggaatc
     3181 tgaaaccagc ttgggcaaca tagtgagatc ccatctctac aaaaaactta aaaattagcc
     3241 aggcatgatg gcacattcct gtagtcctag ctactcagga ggctaaggta ggaggattgc
     3301 ctgagcccag gagttcaagg ctgcagtgag ctaagaacgt gccagtacac tccagcctga
     3361 gccacaaagt gagaccctgt ctcgcaaaaa aaaaaaaaga aagaaaagaa aaagaaaatg
     3421 gctacttctc atgaagccca gattaacttt tctgtgaata tggcctgtga actgttgctg
     3481 gaattaaatt ggagtctagc acaaattaag ttaatctact ctgtattaat cattgggaaa
     3541 aagaaaagct tcatttgaaa acagtctttt tccttcaccc acactaatag aaaaaggaga
     3601 gtaatttgtt catactgtat tccacgtggg atgaaaagca tgttttgctc tttgtttctg
     3661 ggccggtgtg atccgtgtgt tggtgcctga gctggaggaa ggagcttctt gcagggaaac
     3721 agccactggg gccacattga gggccagttg ggaccttcct ttccagtcac actctgtgtc
     3781 ctcacgggcc ccttcacagt ctagataagg agcctagttt cattctcaaa gaaaaagtat
     3841 ttggtcttca acatagaaag cacaaaagcc cagacgagac tgagcacact gttcgaatcc
     3901 actgccagct catctgtgtc agggctggct tctcctgtcc tgtgtcccag ggctgcaggc
     3961 ccgtgttatt tgagtccatc aaatcttccg agggcctcgg aggaaccatt ctcagtcacc
     4021 ccaagctctg taaaaggtca aaacactggc agcctctcag tgcaccactg tgtgggctcc
     4081 acacgggaag gcctggccaa gctgtgctct tagaggctgt cgaggtaggg caggaagctt
     4141 ccctatcctc gtctggatgg aatgaaacag aggttcccta aggccaaggg actcgtggag
     4201 cgcccatccc gggtggggtc ccagaggtga gtgcagcttg cctcctgtcc ccaaggggct
     4261 tgttctgtca ggcctcctcc agtcactgct ggagtgggga ctctgactcc tgtgttcacc
     4321 tcagagccca tgtacaggct agaggtgagg cttggcaaca acttctacta atacttagct
     4381 ttgtggcaga gccagtctga aggaggagaa gcgctctcag ccagcagggg gagtgatgat
     4441 tatcatttca ggtagcaagg atatgtattt actatgtggg gaaataattt gcaactttaa
     4501 tttgtttcca aagcattttg attggcttcc ttttccttta aaccaaaaaa ttgtttaaat
     4561 gtttaagggt catgtggaac agctgagctt ctggcaggac cacagaactg tcctctgagt
     4621 ctgagggctg ggcagtgcca caggacagtc cctgggctgc aggactgtca gtgcttgttg
     4681 tgtgaggaaa tgaggccctt caaaaaccca gtcatcttga cagagggcac actcccaagg
     4741 aaggcctgac tggggaatca tacagcctct cctccaggcc tctgggctca ctgaggtgcc
     4801 cccagaaccc tgattgccct cccctctttc ccttttctca ttcatcacac agtagaagca
     4861 ctcactagct aacagctcag ctggggcagc agcctccatg gaggcctgca agtgatgctc
     4921 ccagtcctgg ttcccgctca ggagggaggc cccaccacgt gcatcactgc ctcagctctg
     4981 caaagtcacc agaggcttct tcctgatttt tgtgaaagat ttattatatg gcaataaatc
     5041 tgaatgataa actttcatta tacttgcatt aattttgagt attagtttgc ttagaaattt
     5101 ttacacgaag taaaaatgtg taatgatgca acacaggatc atcgtgtgtt cagatggaaa
     5161 ttggcatctg attttacccc agatctgaag ctgttactgc attgtgtcat tttatcaaca
     5221 aagacttctg ccacaatatt tacttttaaa agaatttgtc cctttcaata gcatcttccc
     5281 tatcaagagt tcaaggcact ttgcagaaga gcttcagatt tctaactcta gttcccacag
     5341 tcacccgttg aggctggtgt catacaatct atgaacaaca accttgcagc aggactgcca
     5401 ccattcagca gtgcagacaa cacagctcaa ggacaaagac agagaaagga aagaagagaa
     5461 ggtccctgaa ggtgaagcat agaaagtaca ctgcacacac agttctctct ggaaggaaag
     5521 cacaggagct gggaagtaga cgatagaaag tggaaaggac acaggaccag catcagaaca
     5581 ctcagcaact tcctaagagc ttgctaaggt acatccaaga gaaatattta gtgagggaaa
     5641 cttttaagca ctggaattgt taatatatca tactttaagc aacatttcgt cctttagatt
     5701 ccgggagcca gggttttgca ttgggtggta gagctctctg cataattgag tcaccctgtg
     5761 cttttttgca gagccataaa tttttggcac ttgctgatac ttcataggct ggagtctctg
     5821 caaggtcaac cctttgggtc tcttaattct ttcctaggca ctatcctgca atatcagtag
     5881 ttgccacttg attttaggga ctgattatta ggccttctga ctccaaattc agaacaattc
     5941 cagtgtccta catcacctcc tggagtcaaa gaggtcagtt tctattcagt gatgaagagc
     6001 taagctagct atgcatatga agaactgaga agtagattat aacacacaat ccacagcaaa
     6061 taatgcatat ttattttgta tgaattaaag cataaaattt aatctcatta gcgtatagct
     6121 attcagagtt cccactcaaa gcaagggaga ctcaccagta gccccaagac ccttggccag
     6181 gtcatactca acccctaatc taacttcaca aggctgtcta aatgtcagct actcagacac
     6241 agcctctggc attagcaaaa tcccccagag gaaaagcagc cccatagaac tggacaagta
     6301 atacttaact atcttgttga ctctggggtc cttcaagcaa attttaaaaa tatttttacc
     6361 agatttccaa gttgtcctta gcttgagggt tggtcctcca ttactgtcat tggactatcc
     6421 ctcatgggta gtgcagttca acttctaaac cacctcagta cctgaatgtg gtccgaggtg
     6481 aaaccagatt actgatgcct tcaggtgccc aattgaagca aatggcaaat tctgtggagg
     6541 aagtaaacat aacccaaggc ctcaaattat ttctgcagtg ttgcaatcag aaatacccag
     6601 gtatgggaga agaccagaca acattaatta cttgaattaa aaccattaga ggaaaacaca
     6661 gacaatagaa atatccaaac agactccaga taaagttgtc gcagaattta aaacagctat
     6721 ggttactgtg ttcaaaaaaa tgagacgcat ggtcaagaat ttcaacagag agttagaaac
     6781 tgtaaaaggc gcattggaaa ttctataact aaagtactgt aatgagaaga acagtgaaca
     6841 ggtttaagag cagattagaa ataactgaaa agagaatgag taagatgaaa aattgagggt
     6901 gaggggtggg agcaggaatt atatagtaaa aaaaacagaa catggagaat agagggtaag
     6961 agacttaaag ggtacagtga gcatgtctga cctgctcaga catgcagggt agttggcatc
     7021 tcaaaagagt agaagaaagg aatatttaaa caaatggctg agaacttttc caaaactcat
     7081 gaaagatatc acaccaaaaa ttcaagaatc cctcccacaa actccaagtg ggataaataa
     7141 aaacaaccca cacctagaaa catcatagta aaactgttga agaccaaaga aaaataatat
     7201 tttagacctg ctggggtggg agagggaata taaccattaa aggagcaata atattagact
     7261 gacagctcct tctcagcaga aacaatggaa gacagtgaaa tactgccttt tatggttctg
     7321 aaagaaaatg actgccaacc taaaaatttc taatcaggga aaatatcctg caaaaatgaa
     7381 gaggatatga agacattttt agagaaacag ctagaagaat taatcagcac gcctacccta
     7441 aaggaaaatt taaaaggtgt tttacaggca gaaggaaaat aatcccaaac gtaaggacag
     7501 agccctagga aagattgaag agcaatgaaa atgacacata aatattgact acataaaaca
     7561 ataattatgt cttatggagt tttaaatata tagagaaata aaatgtatga aaatgaaagg
     7621 atttatgtgg gaattttaag tgttctaaga cacttggcat catccaagaa gagagtaaaa
     7681 attcatttaa tattagtctt tgataactca tgtatgcatg ataatatttg agatatcaat
     7741 aaaagtgtat agctttccac ataataagag agaattcgaa taatcaattc agtggaagat
     7801 ttaagaattg caaaagagtc taagaaagaa gaaaaagcag aacaggtgga acaaatagaa
     7861 agcaagatat ttgtgataaa tccattatta cactaaatgt aaatggacta atgctcaaat
     7921 gttgacaaat attgtcagcc ttgttttaaa ccatatgcta tttacaagag acatttttag
     7981 aacatgaatt tagaaatttt ggaagaaaaa gatggagaaa tacataccca gcaaacagta
     8041 accatgagga aactggtgta gatatattta agtaaacttt aaggcaataa tttttttaag
     8101 aaataaaagg gtcaattcaa ttactagaaa gataatctaa atttatatgc acttaaaata
     8161 taaactcaaa atatgtaaaa gaaaatttga taggaaattt agacaaatat gcaaccagag
     8221 tgatatttta acatgcctat tttgttaatt ggtagaaaaa ctagcataca aaaatcaagt
     8281 tataaaagat ttaagcaatg aaatgagcaa accaaacgga tgtatgtagg acaccaaact
     8341 caataacata cttttcaaac acaaatggca cacactccaa aatactgggc tataaaggtt
     8401 tcaacaggct aggcacagtg gctcacatct gtaatactag cactttgaga ggccaagtca
     8461 ggaagatcac ttgagcccag gagtttgaga ccagcttggg caacataatg aggccccacc
     8521 tttacaaaaa ataaaaaaat taactgggca tggtggcatg tgcctatagc cccagctact
     8581 tgggaagctg aggcaggaga atcacttgtg cccaggaggt cgaggctgca gtgagctata
     8641 attgcactac tgcactccag cttgggtgac agagcaagac cctgtctcag aaaaaaagtc
     8701 tcaacaaacc tgaaaatact tcaaatgaaa tatgttctct gaccataata gaatcaagac
     8761 taattattta caaaaagata attttataat cctcatattt ttggaaatag atttccaggt
     8821 aacccatctg tcagaatctt aatggaaatt tgaaatattt tgaactgatc atgaaaatac
     8881 tacatatcaa aacagagaaa aaacttgtgg gatgcaaata aaactatgct tagaagacct
     8941 tttaatttta ttttaaattt aaagagtacc tgttcaggtt tgttacaaga atatattgat
     9001 gatgctgagg tttgggctcc taatgatcct gtcacccaga tagtgaacat agtacccaat
     9061 agaaagtttt tcagcacttg cttccctctc tccttccctt cctccctcgt tttggagtcc
     9121 ctagtgtcta ttgttcatat atatatatac acacacatat atatttaaat gtttatgtta
     9181 gaaaagaaga aaggttaaaa atcaattaag caattacctc aaaaatttta aaaacagcac
     9241 atgaaaccca aaaataagcc aaattactga tatagaacac aaatatacaa taaagggaat
     9301 caaagtcaaa agttggttat ttaaaaggat taagaggact cattggctgg gcatgttggc
     9361 tcacatgtgt aatcccagca ctttgggagt cacctgaggt caggagttca agaccagcct
     9421 ggccaacatg atgaaacccc gtctctacta aaaatacaaa aattagctgg gcgtggtggt
     9481 gggcatctgt agtcccagct acgcaggagg ctgaggcaag agaattgctt gaacctggga
     9541 ggtggagggt tcagtgggca gagattgtgc cactgcactc cagcctgggc aacagagcga
     9601 gactccgtcc caaaataaat aaataaaaga attcataacc ccttaaaaga ctgatcaaag
     9661 aaggaaggag aaagggcagg agagaggaat agccaatatc agaaaggaaa acaggtaagt
     9721 tacttcagat tatgcagaca tttaaaatat ttaagagcca gcctggataa catggtgaaa
     9781 ccctcctgtc tctacacaca cacacacaca aattagctga gcatggtggt gtgtgcctgt
     9841 agtcccagct actggagagg ctaaggtagg aggattacct gagcccagga ggtagaggct
     9901 gcagtgagct gtggtcatgg caccacactc cagcctgggc aatggagtga gagaccgtgt
     9961 ctcaaaaata tgtatacata tttaagagaa tattatgaat accaataaat tttaaatcta
    10021 gttgaaatga tctttattgc agtgataggt gtatacatat gtctaatcaa attgtacact
    10081 ttattacata tgtgcagttt tatatattaa gtatgcctca ttaaagttgt ttttagattt
    10141 tgca
//
