LOCUS       NM_020175               2038 bp    mRNA    linear   PRI 21-FEB-2025
DEFINITION  Homo sapiens dihydrouridine synthase 3 like (DUS3L), transcript
            variant 1, mRNA.
ACCESSION   NM_020175
VERSION     NM_020175.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Finet,O., Yague-Sanz,C., Kruger,L.K., Tran,P., Migeot,V.,
            Louski,M., Nevers,A., Rougemaille,M., Sun,J., Ernst,F.G.M.,
            Wacheul,L., Wery,M., Morillon,A., Dedon,P., Lafontaine,D.L.J. and
            Hermand,D.
  TITLE     Transcription-wide mapping of dihydrouridine reveals that mRNA
            dihydrouridylation is required for meiotic chromosome segregation
  JOURNAL   Mol Cell 82 (2), 404-419 (2022)
   PUBMED   34798057
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Dai,W., Li,A., Yu,N.J., Nguyen,T., Leach,R.W., Wuhr,M. and
            Kleiner,R.E.
  TITLE     Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated
            dihydrouridylation
  JOURNAL   Nat Chem Biol 17 (11), 1178-1187 (2021)
   PUBMED   34556860
REFERENCE   3  (bases 1 to 2038)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2038)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK315691.1, AC011499.5 and BC008362.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020175.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225840.1, AK023958.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309061.12/ ENSP00000311977.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-658               AK315691.1         53-710
            659-659             AC011499.5         162847-162847       c
            660-1975            AK315691.1         712-2027
            1976-2038           BC008362.1         1845-1907
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2038
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /note="dihydrouridine synthase 3 like"
                     /db_xref="GeneID:56931"
                     /db_xref="HGNC:HGNC:26920"
                     /db_xref="MIM:621104"
     exon            1..120
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1975
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /EC_number="1.3.1.89"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mRNA-dihydrouridine synthase DUS3L"
                     /codon_start=1
                     /product="tRNA-dihydrouridine(47) synthase
                     [NAD(P)(+)]-like isoform 1"
                     /protein_id="NP_064560.2"
                     /db_xref="CCDS:CCDS32880.1"
                     /db_xref="GeneID:56931"
                     /db_xref="HGNC:HGNC:26920"
                     /db_xref="MIM:621104"
                     /translation="MAEGTAEAPLENGGGGDSGAGALERGVAPIKRQYLTTKEQFHQF
                     LEAKGQEKTCRETEVGDPAGNELAEPEAKRIRLEDGQTADGQTEEAAEPGEQLQTQKR
                     ARGQNKGRPHVKPTNYDKNRLCPSLIQESAAKCFFGDRCRFLHDVGRYLETKPADLGP
                     RCVLFETFGRCPYGVTCRFAGAHLRPEGQNLVQEELAARGTQPPSIRNGLDKALQQQL
                     RKREVRFERAEQALRRFSQGPTPAAAVPEGTAAEGAPRQENCGAQQVPAGPGTSTPPS
                     SPVRTCGPLTDEDVVRLRPCEKKRLDIRGKLYLAPLTTCGNLPFRRICKRFGADVTCG
                     EMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPDTMTKCAELLSRTVEVDFVDI
                     NVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPLTVKIRTGVQERVNLAHRL
                     LPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQAASPMPLFGNGDILSFEDANR
                     AMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTNYGLEHWGSDTQ
                     GVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLETLMASQKAADWI
                     RISEMLLGPVPPSFAFLPKHKANAYK"
     exon            121..409
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            410..922
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            923..964
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1117
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1234
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1300
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1411
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1508
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1584
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1773
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1902
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2038
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2017..2022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /note="hexamer: AATAAA"
     polyA_site      2038
                     /gene="DUS3L"
                     /gene_synonym="DUS3"
                     /note="major polyA site"
ORIGIN      
        1 agagcggatg tgaggggcgc cgatggcgga gggaacggcg gaggctcctc tagagaatgg
       61 tggtggtggc gactcgggag ccggagcttt ggaacgagga gtggcgccca ttaagcgtca
      121 atacctcacc accaaggagc agtttcacca attcctggaa gccaaagggc aggagaagac
      181 ttgccgggaa accgaggtag gagaccctgc tggcaatgag ctggctgagc ctgaggctaa
      241 gcggatccga ctggaggatg gacagacggc ggacgggcag acggaggagg cagcagagcc
      301 cggggagcag ctacagactc agaagagggc ccggggacaa aacaagggcc ggccccatgt
      361 gaagcccacg aactacgaca agaacaggct gtgtccctcc ctaatccagg agtcggctgc
      421 taagtgtttc ttcggtgatc gctgccgctt tctgcacgac gtggggcgct acctggagac
      481 caagccggcc gacctgggcc cccgctgcgt gctcttcgag accttcggcc ggtgccccta
      541 cggcgtgacc tgccgcttcg ctggggccca cctgaggccc gagggacaga acctggtgca
      601 ggaggagttg gcggcccgcg ggacccagcc cccgtccatc cgcaacggcc tggacaaagc
      661 cctgcagcag cagctgcgga agcgcgaggt ccgcttcgag cgagctgagc aggccctgcg
      721 ccggttcagc cagggcccca cacccgctgc cgctgtcccc gagggcacgg cagccgaggg
      781 cgctcccagg caggaaaact gtggtgccca gcaggtcccc gcagggccgg gcactagcac
      841 ccctcccagc agccccgtgc ggacctgcgg gcccctgacg gatgaggacg tggtcaggct
      901 gcggccctgt gagaagaagc ggctggacat ccgtggcaaa ctttacctgg cccccctcac
      961 cacgtgtggg aacctgccct tccgacggat ctgcaagcgc ttcggggcgg atgtgacatg
     1021 tggagagatg gccgtctgca ccaacctgct gcagggccag atgtccgagt gggccctact
     1081 caaacgccac cagtgtgagg acatctttgg cgtccagctg gagggcgcct tccccgacac
     1141 catgaccaag tgtgccgagc tgctgagccg caccgtggag gtggactttg tggacatcaa
     1201 cgtcggctgc cccatcgacc tcgtgtacaa gaagggtggg ggctgtgccc tcatgaatcg
     1261 ctccaccaag ttccagcaga tcgtccgtgg catgaaccag gtgctggatg tgccgctgac
     1321 tgtgaagatc cgcacaggcg tccaggagcg tgtgaacctg gcgcaccgcc tgctgcccga
     1381 gctgcgggac tggggcgtgg cactcgtcac gctccacggc cgctctcggg agcagcgcta
     1441 caccaagcta gccgactggc agtacatcga ggagtgcgtg caggccgcca gccccatgcc
     1501 cctgttcgga aatggggaca tcttgtcatt tgaggatgcc aaccgcgcca tgcagactgg
     1561 tgtcaccggg atcatgattg cccgtggcgc cctgctcaag ccgtggctct tcacggagat
     1621 caaggagcag cggcactggg acatctcgtc gtccgagcgc ctggacatcc tgcgggactt
     1681 caccaactac ggcctggagc actggggctc ggacacgcag ggcgtggaga agacccggcg
     1741 ctttctgctc gagtggctgt ccttcctgtg ccggtacgtg cccgtggggc tgctggagcg
     1801 gctcccacag aggatcaacg agcggccgcc ctactacctg ggccgcgact acctggagac
     1861 gctgatggcc agccagaagg cagccgactg gatccgcatc agcgagatgc tccttgggcc
     1921 agtgcccccc agcttcgcct tcttgccgaa gcacaaggcc aacgcgtaca agtagcctca
     1981 ggctttccca ggggcaccct ggggcgagga gagtacaata aattttattc ttttaaaa
//
