LOCUS       NM_017803               1982 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens dihydrouridine synthase 2 (DUS2), transcript variant
            1, mRNA.
ACCESSION   NM_017803
VERSION     NM_017803.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ji,J., Yu,N.J. and Kleiner,R.E.
  TITLE     Sequence- and Structure-Specific tRNA Dihydrouridylation by hDUS2
  JOURNAL   ACS Cent Sci 10 (4), 803-812 (2024)
   PUBMED   38680565
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Finet,O., Yague-Sanz,C., Kruger,L.K., Tran,P., Migeot,V.,
            Louski,M., Nevers,A., Rougemaille,M., Sun,J., Ernst,F.G.M.,
            Wacheul,L., Wery,M., Morillon,A., Dedon,P., Lafontaine,D.L.J. and
            Hermand,D.
  TITLE     Transcription-wide mapping of dihydrouridine reveals that mRNA
            dihydrouridylation is required for meiotic chromosome segregation
  JOURNAL   Mol Cell 82 (2), 404-419 (2022)
   PUBMED   34798057
REFERENCE   3  (bases 1 to 1982)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 1982)
  AUTHORS   Bou-Nader,C., Barraud,P., Pecqueur,L., Perez,J., Velours,C.,
            Shepard,W., Fontecave,M., Tisne,C. and Hamdane,D.
  TITLE     Molecular basis for transfer RNA recognition by the double-stranded
            RNA-binding domain of human dihydrouridine synthase 2
  JOURNAL   Nucleic Acids Res 47 (6), 3117-3126 (2019)
   PUBMED   30605527
  REMARK    GeneRIF: we establish that while hDus2 dsRBD retains a conventional
            dsRNA recognition capability, the presence of an N-terminal
            extension appended to the canonical domain provides additional
            residues for binding tRNA in a structure-specific mode of action.
REFERENCE   5  (bases 1 to 1982)
  AUTHORS   Bou-Nader,C., Pecqueur,L., Bregeon,D., Kamah,A., Guerineau,V.,
            Golinelli-Pimpaneau,B., Guimaraes,B.G., Fontecave,M. and Hamdane,D.
  TITLE     An extended dsRBD is required for post-transcriptional modification
            in human tRNAs
  JOURNAL   Nucleic Acids Res 43 (19), 9446-9456 (2015)
   PUBMED   26429968
  REMARK    GeneRIF: This is the first reported case of a tRNA-modifying enzyme
            (dihydrouridine synthase 2) carrying a dsRNA binding domain used to
            bind tRNAs.
REFERENCE   6  (bases 1 to 1982)
  AUTHORS   Kasprzak,J.M., Czerwoniec,A. and Bujnicki,J.M.
  TITLE     Molecular evolution of dihydrouridine synthases
  JOURNAL   BMC Bioinformatics 13, 153 (2012)
   PUBMED   22741570
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1982)
  AUTHORS   Griffiths,S., Byrne,R.T., Antson,A.A. and Whelan,F.
  TITLE     Crystallization and preliminary X-ray crystallographic analysis of
            the catalytic domain of human dihydrouridine synthase
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun 68 (Pt 3), 333-336
            (2012)
   PUBMED   22442237
  REMARK    GeneRIF: crystals belonged to space group P2(1) and contained a
            single molecule of HsDus2L in the asymmetric unit
REFERENCE   8  (bases 1 to 1982)
  AUTHORS   Luo,X., Wang,C.Z., Chen,J., Song,W.X., Luo,J., Tang,N., He,B.C.,
            Kang,Q., Wang,Y., Du,W., He,T.C. and Yuan,C.S.
  TITLE     Characterization of gene expression regulated by American ginseng
            and ginsenoside Rg3 in human colorectal cancer cells
  JOURNAL   Int J Oncol 32 (5), 975-983 (2008)
   PUBMED   18425323
REFERENCE   9  (bases 1 to 1982)
  AUTHORS   Mittelstadt,M., Frump,A., Khuu,T., Fowlkes,V., Handy,I., Patel,C.V.
            and Patel,R.C.
  TITLE     Interaction of human tRNA-dihydrouridine synthase-2 with
            interferon-induced protein kinase PKR
  JOURNAL   Nucleic Acids Res 36 (3), 998-1008 (2008)
   PUBMED   18096616
  REMARK    GeneRIF: hDUS2 interacts with protein kinase PKR through its dsRNA
            binding/dimerization domain and inhibits its kinase activity.
REFERENCE   10 (bases 1 to 1982)
  AUTHORS   Kato,T., Daigo,Y., Hayama,S., Ishikawa,N., Yamabuki,T., Ito,T.,
            Miyamoto,M., Kondo,S. and Nakamura,Y.
  TITLE     A novel human tRNA-dihydrouridine synthase involved in pulmonary
            carcinogenesis
  JOURNAL   Cancer Res 65 (13), 5638-5646 (2005)
   PUBMED   15994936
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC130462.3, BC006527.2 and
            AK000406.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017803.4.
            
            Summary: This gene encodes a cytoplasmic protein that catalyzes the
            conversion of uridine residues to dihydrouridine in the D-loop of
            tRNA. The resulting modified bases confer enhanced regional
            flexibility to tRNA. The encoded protein may increase the rate of
            translation by inhibiting an interferon-induced protein kinase.
            This gene has been implicated in pulmonary carcinogenesis.
            Alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.210121.1,
                                           SRR3476690.650714.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000565263.6/ ENSP00000455229.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC130462.3         14759-14775
            18-1942             BC006527.2         1-1925
            1943-1982           AK000406.1         1850-1889
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..1982
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /note="dihydrouridine synthase 2"
                     /db_xref="GeneID:54920"
                     /db_xref="HGNC:HGNC:26014"
                     /db_xref="MIM:609707"
     exon            1..68
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            69..148
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            149..292
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1648
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /EC_number="1.3.1.91"
                     /note="isoform 1 is encoded by transcript variant 1;
                     up-regulated in lung cancer protein 8; dihydrouridine
                     synthase 2-like, SMM1 homolog; tRNA-dihydrouridine(20)
                     synthase (NAD(P)(+))"
                     /codon_start=1
                     /product="tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
                     isoform 1"
                     /protein_id="NP_060273.1"
                     /db_xref="CCDS:CCDS10859.1"
                     /db_xref="GeneID:54920"
                     /db_xref="HGNC:HGNC:26014"
                     /db_xref="MIM:609707"
                     /translation="MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEEL
                     IDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARL
                     VENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILP
                     SLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSH
                     DHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDN
                     HYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARL
                     SAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQ
                     RPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHK
                     RKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW"
     exon            293..338
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            339..430
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            431..474
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            475..535
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            536..583
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            584..649
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            650..720
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            721..807
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            808..976
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1098
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1248
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1336
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1410
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1982
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1921..1926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /note="hexamer: AATAAA"
     polyA_site      1943
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
     polyA_site      1982
                     /gene="DUS2"
                     /gene_synonym="DUS2L; SMM1; URLC8"
                     /note="major polyA site"
ORIGIN      
        1 agtacggtgt gtggagctgg agcaccgtga ggaagaagcg aggttctttt taagagttca
       61 gctgcgagat atcaaacaaa gaattactct gtacaaagcc agaacacata tatcaaagta
      121 atcctgaagt atcagaacaa aataataggc tgtaacagag gaggaaatga ttttgaatag
      181 cctctctctg tgttaccata ataagctaat cctggcccca atggttcggg tagggactct
      241 tccaatgagg ctgctggccc tggattatgg agcggacatt gtttactgtg aggagctgat
      301 cgacctcaag atgattcagt gcaagagagt tgttaatgag gtgctcagca cagtggactt
      361 tgtcgcccct gatgatcgag ttgtcttccg cacctgtgaa agagagcaga acagggtggt
      421 cttccagatg gggacttcag acgcagagcg agcccttgct gtggccaggc ttgtagaaaa
      481 tgatgtggct ggtattgatg tcaacatggg ctgtccaaaa caatattcca ccaagggagg
      541 aatgggagct gccctgctgt cagaccctga caagattgag aagatcctca gcactcttgt
      601 taaagggaca cgcagacctg tgacctgcaa gattcgcatc ctgccatcgc tagaagatac
      661 cctgagcctt gtgaagcgga tagagaggac tggcattgct gccatcgcag ttcatgggag
      721 gaagcgggag gagcgacctc agcatcctgt cagctgtgaa gtcatcaaag ccattgctga
      781 taccctctcc attcctgtca tagccaacgg aggatctcat gaccacatcc aacagtattc
      841 ggacatagag gactttcgac aagccacggc agcctcttcc gtgatggtgg cccgagcagc
      901 catgtggaac ccatctatct tcctcaagga gggtctgcgg cccctggagg aggtcatgca
      961 gaaatacatc agatacgcgg tgcagtatga caaccactac accaacacca agtactgctt
     1021 gtgccagatg ctacgagaac agctggagtc gccccaggga aggttgctcc atgctgccca
     1081 gtcttcccgg gaaatttgtg aggcctttgg ccttggtgcc ttctatgagg agaccacaca
     1141 ggagctggat gcccagcagg ccaggctctc agccaagact tcagagcaga caggggagcc
     1201 agctgaagat acctctggtg tcattaagat ggctgtcaag tttgaccgga gagcataccc
     1261 agcccagatc acccctaaga tgtgcctact agagtggtgc cggagggaga agttggcaca
     1321 gcctgtgtat gaaacggttc aacgccctct agatcgcctg ttctcctcta ttgtcaccgt
     1381 tgctgaacaa aagtatcagt ctaccttgtg ggacaagtcc aagaaactgg cggagcaggc
     1441 tgcagccatc gtctgtctgc ggagccaggg cctccctgag ggtcggctgg gtgaggagag
     1501 cccttccttg cacaagcgaa agagggaggc tcctgaccaa gaccctgggg gccccagagc
     1561 tcaggagcta gcacaacctg gggatctgtg caagaagccc tttgtggcct tgggaagtgg
     1621 tgaagaaagc cccctggaag gctggtgact actcttcctg ccttagtcac ccctccatgg
     1681 gcctggtgct aaggtggctg tggatgccac agcatgaacc agatgccgtt gaacagtttg
     1741 ctggtcttgc ctggcagaag ttagatgtcc tggcaggggc catcagccta gagcatggac
     1801 caggggccgc ccaggggtgg atcctggccc ctttggtgga tctgagtgac agggtcaagt
     1861 tctctttgaa aacaggagct tttcaggtgg taactcccca acctgacatt ggtactgtgc
     1921 aataaagaca ccccctaccc tcacccacgg ctggctgctt cagccttggg catcttcata
     1981 aa
//
