LOCUS       NM_173666               5776 bp    mRNA    linear   PRI 27-FEB-2025
DEFINITION  Homo sapiens DTW domain containing 2 (DTWD2), transcript variant 1,
            mRNA.
ACCESSION   NM_173666
VERSION     NM_173666.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Xie,Y., Chai,P., Till,N.A., Hemberger,H., Lebedenko,C.G., Porat,J.,
            Watkins,C.P., Caldwell,R.M., George,B.M., Perr,J., Bertozzi,C.R.,
            Garcia,B.A. and Flynn,R.A.
  TITLE     The modified RNA base acp3U is an attachment site for N-glycans in
            glycoRNA
  JOURNAL   Cell 187 (19), 5228-5237 (2024)
   PUBMED   39173631
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Takakura,M., Ishiguro,K., Akichika,S., Miyauchi,K. and Suzuki,T.
  TITLE     Biogenesis and functions of aminocarboxypropyluridine in tRNA
  JOURNAL   Nat Commun 10 (1), 5542 (2019)
   PUBMED   31804502
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5776)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   4  (bases 1 to 5776)
  AUTHORS   Pan,Y., Luo,X., Liu,X., Wu,L.Y., Zhang,Q., Wang,L., Wang,W., Zuo,L.
            and Wang,K.S.
  TITLE     Genome-wide association studies of maximum number of drinks
  JOURNAL   J Psychiatr Res 47 (11), 1717-1724 (2013)
   PUBMED   23953852
  REMARK    GeneRIF: The rs17144687 near DTWD2 associated with MaxDirnks.
REFERENCE   5  (bases 1 to 5776)
  AUTHORS   Fox,C.S., Liu,Y., White,C.C., Feitosa,M., Smith,A.V.,
            Heard-Costa,N., Lohman,K., Johnson,A.D., Foster,M.C.,
            Greenawalt,D.M., Griffin,P., Ding,J., Newman,A.B., Tylavsky,F.,
            Miljkovic,I., Kritchevsky,S.B., Launer,L., Garcia,M.,
            Eiriksdottir,G., Carr,J.J., Gudnason,V., Harris,T.B., Cupples,L.A.
            and Borecki,I.B.
  CONSRTM   GIANT Consortium; MAGIC Consortium; GLGC Consortium
  TITLE     Genome-wide association for abdominal subcutaneous and visceral
            adipose reveals a novel locus for visceral fat in women
  JOURNAL   PLoS Genet 8 (5), e1002695 (2012)
   PUBMED   22589738
REFERENCE   6  (bases 1 to 5776)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 5776)
  AUTHORS   Manrow,R.E., Leone,A., Krug,M.S., Eschenfeldt,W.H. and Berger,S.L.
  TITLE     The human prothymosin alpha gene family contains several processed
            pseudogenes lacking deleterious lesions
  JOURNAL   Genomics 13 (2), 319-331 (1992)
   PUBMED   1612591
REFERENCE   8  (bases 1 to 5776)
  AUTHORS   Eschenfeldt,W.H., Manrow,R.E., Krug,M.S. and Berger,S.L.
  TITLE     Isolation and partial sequencing of the human prothymosin alpha
            gene family. Evidence against export of the gene products
  JOURNAL   J Biol Chem 264 (13), 7546-7555 (1989)
   PUBMED   2708378
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY055818.1, BC114508.1, AC010462.6 and AW009893.1.
            
            On Nov 22, 2018 this sequence version replaced NM_173666.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.506931.1,
                                           SRR1660803.3912.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000510708.6/ ENSP00000425048.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 HY055818.1         60-65
            7-1281              BC114508.1         1-1275
            1282-5625           AC010462.6         88411-92754
            5626-5776           AW009893.1         16-166              c
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q23.1"
     gene            1..5776
                     /gene="DTWD2"
                     /note="DTW domain containing 2"
                     /db_xref="GeneID:285605"
                     /db_xref="HGNC:HGNC:19334"
                     /db_xref="MIM:621117"
     exon            1..254
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..933
                     /gene="DTWD2"
                     /EC_number="2.5.1.25"
                     /note="isoform a is encoded by transcript variant 1; DTW
                     domain-containing protein 2; tRNA-uridine
                     aminocarboxypropyltransferase 2"
                     /codon_start=1
                     /product="tRNA-uridine aminocarboxypropyltransferase 2
                     isoform a"
                     /protein_id="NP_775937.1"
                     /db_xref="CCDS:CCDS34216.1"
                     /db_xref="GeneID:285605"
                     /db_xref="HGNC:HGNC:19334"
                     /db_xref="MIM:621117"
                     /translation="MESQKEARTLQEPVARPSGASSSQTPNDKERREGGAVPAAAALG
                     AEADDDSADGLWELPVEPAERRPECTRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPA
                     EENKVLRTVPLLAACLPQDKCKVKIGRRFSEERDPELSTVCRKSGTLILYPGAEAANL
                     EEFILDSPVYPSTIIIIDGTWSQAKDIFYKNSLFRHPKQVQLKTSISSQYVIRMQPTN
                     RCLSTLECAAVALSILEKNNYIQETLLRPLQALCSFQLQHGAQIRLSKEHLLKNGLYP
                     KPMPKNKRKLRKMELLMNSVKI"
     exon            255..345
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     exon            346..440
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     exon            441..633
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     exon            634..762
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     exon            763..5776
                     /gene="DTWD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1095..1100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DTWD2"
                     /note="hexamer: AATAAA"
     polyA_site      1125
                     /gene="DTWD2"
                     /note="major polyA site"
     regulatory      4508..4513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DTWD2"
                     /note="hexamer: AATAAA"
     regulatory      4512..4517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DTWD2"
                     /note="hexamer: AATAAT"
     polyA_site      4528
                     /gene="DTWD2"
                     /note="major polyA site"
     polyA_site      4535
                     /gene="DTWD2"
     regulatory      5755..5760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DTWD2"
                     /note="hexamer: ATTAAA"
     polyA_site      5776
                     /gene="DTWD2"
                     /note="major polyA site"
ORIGIN      
        1 gggcttcaat gccacggcct gaccggagtg tccgccatgg agtcgcagaa agaggcacga
       61 acactccagg agcccgttgc gcggccttct ggggcctcaa gctctcagac gccgaacgac
      121 aaggagcggc gggagggcgg cgcagtgccg gcggcggctg ccctgggcgc agaggcggac
      181 gacgacagtg cggacgggct gtgggagctg ccggtggagc cggccgagcg gaggcctgag
      241 tgcacccgct gcagccggcc tcagaaagtg tgtttgtgtc catttctccc agcgcaccct
      301 ctgcatatct ctacccactt gtacataatt cagcatccag cagaggaaaa caaagtgttg
      361 cgtacagttc ctctactagc agcatgcctc ccccaggaca agtgtaaagt gaagatcggt
      421 cgtcgcttca gtgaagaaag agatcctgaa ctttcaactg tttgccggaa gtctggtaca
      481 ttaatattat atccaggggc tgaagctgct aatttggaag aatttatatt agattctcct
      541 gtttatcctt ctacaatcat catcattgat ggtacatgga gccaggctaa ggacattttc
      601 tataagaact ccttgttccg acatcccaaa caggtgcaat taaaaactag catttctagt
      661 cagtatgtaa ttcggatgca gccgactaat agatgccttt ctacactgga gtgtgcagct
      721 gttgctcttt ccatcttgga gaaaaataat tacatacaag agactttgct tcgccctctt
      781 caagctttat gctcctttca acttcagcat ggtgcccaaa ttcgcctcag caaggaacac
      841 cttctgaaaa atggattata tcctaaacca atgccaaaga acaaacgcaa actcaggaaa
      901 atggaattgt taatgaatag tgttaaaatt tagtacagtt attcttttgg tgctagctta
      961 actgtcttca tctaacaata ccaaattaag ttttcatata gttaaggtct gtaggttttt
     1021 gacttgctaa agaaggagaa ttgcatacta atcttaccca gaagaaggaa gtaaaccaaa
     1081 tattcataaa tacaaataaa cagaaattca ctggctcact gaaaagcagg atttcctact
     1141 gtacttgttt ttaaaaatgt gcctctaata catgttttca aacccaaatt taaaatatga
     1201 tttgttgttg tgataggtta gaagttctta cttgtaaaat agaagaaaat tgttgtagtg
     1261 gattggaacg gtgttgtgtc cattaatgtt tataaataat taggtgtaga ccaataatat
     1321 aagattccta catcagtttg cagtcctgac tttacagttt gaaataagta gcaaaagaaa
     1381 attatgataa tcaagtaatt tattattttg taaaatagta taaaggaata aggaaatttt
     1441 aagtattgtt taggggccaa atttgaactc agctaatatg atttaagcgc tctattaatt
     1501 gccctgattt ttaaagaggt gcatgtaatt ttgaaatagc acacattttc atttgtaatc
     1561 actctggata aatttgtatt tttcagtgtt cattgaacag gcttattgac tctggtaaac
     1621 ttattttatt gatgttttca tatggaaaat aataagtgga atatgataca attgttattt
     1681 tcagaacata tttctttagg gctatttaat ataacctttt taacgagcaa aaactttcca
     1741 tttgagagaa aaaattcttc ctgctctgtg ggaaaatatt tgctgagatt ttgtatagaa
     1801 taagagacat gtatgtaaca tatatgtata ttcagcacaa gtctactgca attatgtaca
     1861 cattttggca ggaagagggg attttgttta gtctttgttt atgagttcta gagtttcctg
     1921 tatctagtgt taagttgtaa ggaaaaacta aacatgccat ttaaaggtaa acttggtaac
     1981 tatttataga gcatacacca atgattattt ctcacagttt tcatgatcat ttgtttatta
     2041 tttacttgga ttagactcat taaaaggtat aatgctggtc acaattagaa tgctaatatt
     2101 tggggaaact atgcagaaaa tattttaaaa tattaaaaga gtgctggctt ctattgttga
     2161 tttatttttg gatttaaaaa caaattaggg ctgggcccat tagctcacac ctgtaatccc
     2221 agcactttgg gaggctgaga tgtgagaatt gcttgagccc aggagtttga gaccagcctg
     2281 ggcaatatgg tgagactcca tctccataca aaattaaaaa aaaaataatt agccagatgt
     2341 ggtggtgtgc acttgtagtc ctagctactt gggaggctaa ggtgggagga tcgctggagc
     2401 ccaggacatc aaggttgcaa ggagctatgt tcattccact gcactccagc ctgggctaca
     2461 gagtgagacc tgtctaaaat aaataaataa ataaacaaac tagctcattt acaatgtaaa
     2521 gcacttaata tcaaatgatt atttggtgta ctagaaaaga aacagcatat aataatgtat
     2581 aatagtttct taacaagtgg ggctaaaatc attttgtagt aatttttcac attgatatta
     2641 aacttcatga gtttctttta gtcattatta ttattattat tattatttga gatggaatct
     2701 cgctctgtcg cccaggctgg agtgcagtgg cgcaatcttg gctcactgaa agctctgcct
     2761 cctgggttca cgccattctt ctgcctcagc ctcctgagta gctgggacta caggtgccca
     2821 ccaccacgcc cggctaattt tttgtatttt tagtagagat ggggtttcac cgtattagcc
     2881 aggatggtct cgatctcctg acctcgtgat ctgtctgcct tggcctccaa aagtgctggg
     2941 accgtgccca gccatcatta ttatttttta aaaagaaaac tccccctacc cccagtatac
     3001 cattttgtat ctcaaaattt tactttattg atttttaaga gtaatttcta cttttatctt
     3061 ttaaataaaa cattttagag tatttttgaa gacttaaaaa gcttaaaatt tagtgttttt
     3121 ggaaatgtat aaaattttct tgaagtatgt atagttttcc aaattacttt actgacctgt
     3181 taactcactg tacttgtttt ttacttattt tttcctgacc tcttaacaag gtgagtccat
     3241 ttaatcatct gtggtataaa atgcttctct agtcacctgt tctcattaaa cagttgtaag
     3301 taagttttaa aagccattat tttaattact aaaagttctc aagtgtgtag tattatctga
     3361 gtattttaat tgaaaaatta atcaggcttt taaaaattac ttttttattg tattagttgg
     3421 cttttaaatt cagattcttt atatttgctt gatttttcct ggaagttcca agttttatga
     3481 atttgtagct gatagtaggt tttacttgtg taccccatct gcactttgat gttgtgtaga
     3541 tcgttttgtg agttatttga accgtgtaac tcagagaata gtatttcctc atacataaag
     3601 tgacctcact tttaatcagc tcctttatag tatgttgaaa atcactgcgt gtaatgagag
     3661 gtattgagag tttcttgcac acatgagtgt aaaaggttga gaactagatt atacaaagta
     3721 gtaagtcaca gtgcatggca actttctaca ggttggatta taagacttat gtggaatagg
     3781 agaattatat tgggaaaaaa ttattttctc taacttgaag actccagaga cacttttctt
     3841 agtcatgacc agtaatattt ttcatatatt ttggaagaaa taagctcctc actataccac
     3901 cagttagtga tttatttatt tatgtatttt agagacaggg cctccttctg tcacccagtt
     3961 tggagtgcag tggtgtaatc atagcttact gcagcatcaa actcctgggc tcaagtgttc
     4021 ctcctgcctc agcctcccaa gtagctagaa cctcagcccc atgccaccac actcagctaa
     4081 ttttaaattt tactaatttc atttaatttt tgtggagatg ggggtcttgc tgtgttgttt
     4141 aggctggtcc tgaactcctg gcctcaagcg atccttctgc cttggcctct cagtaaatta
     4201 tatagaagag cacttctgta taatttactc agtttcttca tcacatattt ccaactgttg
     4261 ccctaaatag gtttaaaaat gtgatcatgt tattagaaaa tcctttccat tgaatttcag
     4321 ggatgctgaa tttcagggag ctaaattgtt gacccttgcc tgtagttgtt attggttcag
     4381 aagtaaattt tattgatgct acaagtaaaa aattagatct gacttagtaa actcaaaagt
     4441 tttacaaaac tgtattttac tatgtagatg tatttcttta aaataggtat ggatggatat
     4501 ttttatgaat aaataatatt gcattataga tggaatgaaa ctttgcattt aatttgcccc
     4561 ataagtaggc tttttgtaga atgaattact ctcttaaatt gtccaatcta aatttttatt
     4621 agctacatta acttaaagca tattctctct cttttctctt cacactttct tcctccttcc
     4681 cagggtcttc ctctccctct ttcatcacct acattgttcc ccttcccttt tatactattc
     4741 ttattgtatg tactagctgt tgttatttat acaaatactc atacatcctt acatagcatg
     4801 tttttaaaca aaagatactg tagtggtctt aacagtttac aattttatat ttaaaaatta
     4861 ttcaggaaat ctggaaatcc ctgacttttt acaccgtgct gatcacttga ctcctgtctt
     4921 agtccattct ggctgctctc tcagaatacc agagactgga tagcttataa aaaacagaaa
     4981 tttatttctt ctaagtctgg aggctgggaa atacaatatc aaggtgccag cagatttggt
     5041 gtctggtgag gggcccactc actggttcat agatggtgca ttctagctgt gtcttcacct
     5101 agtggaaggg tcgagctagc tctctgagac ccttatgacc taatcatccc ccaaaggttc
     5161 cacttcctaa tactatacct cgggaattaa ggtttcaacc tgtgagtttt gtggggacac
     5221 aaatattcag accatagcaa ctcctgtact ctactaaaaa gcatattaga gaaataagta
     5281 attgtttttc aatttttaag aattattcac ttgagccaag cgtggtggct cacatctgta
     5341 attccagcac tttgggaggc cgaggtgggt agatcacgag gtcaggagtt caagaccagc
     5401 ctggccaaga tggtgaaacc tcatctgtac taaaaataca aaaattagcc aggcgtagtg
     5461 gcaggcacct gtaatcccag ctactcagga ggctgaggca gagaattgct tgaacctggg
     5521 aggcagaggt tgcagtgagc tgagatgtac cactgcactc tagcctgggc gacagagtga
     5581 gacagtgtct caaataaata aataaataaa taaaaataag attcacttga actaacaacg
     5641 taagaattat ttttaaaaga aactttttaa tgtaaggttt tttaaaaaaa ttattcgaat
     5701 aagaaatttt actatatgtc ttactgttat tgttacttag aagtatatta aagaattaaa
     5761 acatttttta aaagtg
//
