LOCUS       NM_006870               3405 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens destrin, actin depolymerizing factor (DSTN),
            transcript variant 1, mRNA.
ACCESSION   NM_006870
VERSION     NM_006870.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Wen,R., Zhou,L., Jiang,S., Fan,H., Zheng,K., Yu,Y., Gao,X., Hao,L.,
            Lou,Z., Yu,G., Yang,F. and Zhang,W.
  TITLE     DSTN Hypomethylation Promotes Radiotherapy Resistance of Rectal
            Cancer by Activating the Wnt/beta-Catenin Signaling Pathway
  JOURNAL   Int J Radiat Oncol Biol Phys 117 (1), 198-210 (2023)
   PUBMED   37019366
  REMARK    GeneRIF: DSTN Hypomethylation Promotes Radiotherapy Resistance of
            Rectal Cancer by Activating the Wnt/beta-Catenin Signaling Pathway.
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3405)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   4  (bases 1 to 3405)
  AUTHORS   Liao,K.A., Rangarajan,K.V., Bai,X., Taylor,J.M. and Mack,C.P.
  TITLE     The actin depolymerizing factor destrin serves as a negative
            feedback inhibitor of smooth muscle cell differentiation
  JOURNAL   Am J Physiol Heart Circ Physiol 321 (5), H893-H904 (2021)
   PUBMED   34559579
  REMARK    GeneRIF: The actin depolymerizing factor destrin serves as a
            negative feedback inhibitor of smooth muscle cell differentiation.
REFERENCE   5  (bases 1 to 3405)
  AUTHORS   Ullo,M.F. and Logue,J.S.
  TITLE     ADF and cofilin-1 collaborate to promote cortical actin flow and
            the leader bleb-based migration of confined cells
  JOURNAL   Elife 10, e67856 (2021)
   PUBMED   34169836
  REMARK    GeneRIF: ADF and cofilin-1 collaborate to promote cortical actin
            flow and the leader bleb-based migration of confined cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3405)
  AUTHORS   Toshima,J., Toshima,J.Y., Takeuchi,K., Mori,R. and Mizuno,K.
  TITLE     Cofilin phosphorylation and actin reorganization activities of
            testicular protein kinase 2 and its predominant expression in
            testicular Sertoli cells
  JOURNAL   J Biol Chem 276 (33), 31449-31458 (2001)
   PUBMED   11418599
REFERENCE   7  (bases 1 to 3405)
  AUTHORS   Hatanaka,H., Ogura,K., Moriyama,K., Ichikawa,S., Yahara,I. and
            Inagaki,F.
  TITLE     Tertiary structure of destrin and structural similarity between two
            actin-regulating protein families
  JOURNAL   Cell 85 (7), 1047-1055 (1996)
   PUBMED   8674111
REFERENCE   8  (bases 1 to 3405)
  AUTHORS   Agnew,B.J., Minamide,L.S. and Bamburg,J.R.
  TITLE     Reactivation of phosphorylated actin depolymerizing factor and
            identification of the regulatory site
  JOURNAL   J Biol Chem 270 (29), 17582-17587 (1995)
   PUBMED   7615564
REFERENCE   9  (bases 1 to 3405)
  AUTHORS   Hawkins,M., Pope,B., Maciver,S.K. and Weeds,A.G.
  TITLE     Human actin depolymerizing factor mediates a pH-sensitive
            destruction of actin filaments
  JOURNAL   Biochemistry 32 (38), 9985-9993 (1993)
   PUBMED   8399167
REFERENCE   10 (bases 1 to 3405)
  AUTHORS   Moriyama,K., Nishida,E., Yonezawa,N., Sakai,H., Matsumoto,S.,
            Iida,K. and Yahara,I.
  TITLE     Destrin, a mammalian actin-depolymerizing protein, is closely
            related to cofilin. Cloning and expression of porcine brain destrin
            cDNA
  JOURNAL   J Biol Chem 265 (10), 5768-5773 (1990)
   PUBMED   2156828
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC009477.2, S65738.1,
            AI597935.1 and AL132765.38.
            
            On Nov 22, 2018 this sequence version replaced NM_006870.3.
            
            Summary: The product of this gene belongs to the actin-binding
            proteins ADF family. This family of proteins is responsible for
            enhancing the turnover rate of actin in vivo. This gene encodes the
            actin depolymerizing protein that severs actin filaments (F-actin)
            and binds to actin monomers (G-actin). Two transcript variants
            encoding distinct isoforms have been identified for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript, but encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.145469.1,
                                           SRR1803615.81795.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246069.12/ ENSP00000246069.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-813               BC009477.2         24-836
            814-1340            S65738.1           752-1278
            1341-1485           S65738.1           1280-1424
            1486-1493           AI597935.1         1-8                 c
            1494-3405           AL132765.38        54364-56275
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.1"
     gene            1..3405
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="destrin, actin depolymerizing factor"
                     /db_xref="GeneID:11034"
                     /db_xref="HGNC:HGNC:15750"
                     /db_xref="MIM:609114"
     exon            1..137
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..632
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="isoform a is encoded by transcript variant 1;
                     bA462D18.2 (destrin (actin depolymerizing factor ADF)
                     (ACTDP)); actin-depolymerizing factor; epididymis luminal
                     protein 32; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="destrin isoform a"
                     /protein_id="NP_006861.1"
                     /db_xref="CCDS:CCDS13127.1"
                     /db_xref="GeneID:11034"
                     /db_xref="HGNC:HGNC:15750"
                     /db_xref="MIM:609114"
                     /translation="MASGVQVADEVCRIFYDMKVRKCSTPEEIKKRKKAVIFCLSADK
                     KCIIVEEGKEILVGDVGVTITDPFKHFVGMLPEKDCRYALYDASFETKESRKEELMFF
                     LWAPELAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEKLGGSLIVAF
                     EGCPV"
     exon            138..445
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /inference="alignment:Splign:2.1.0"
     exon            446..522
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /inference="alignment:Splign:2.1.0"
     exon            523..3405
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1468..1473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="hexamer: AATAAA"
     polyA_site      1486
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="major polyA site"
     regulatory      1705..1710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="hexamer: AATAAA"
     polyA_site      1728
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="major polyA site"
     regulatory      3381..3386
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
                     /note="hexamer: AATAAA"
     polyA_site      3405
                     /gene="DSTN"
                     /gene_synonym="ACTDP; ADF; bA462D18.2; HEL32"
ORIGIN      
        1 gccccggggt aagctcgcgc cgccgcgtca gctcagcgct gggtctctcg gtcccgcagc
       61 cgtgaggagg acggtctgca tactcgctgc ccgccggctc cctcccccgc gtccctgcga
      121 ccgccgcggc gaagatggcc tcaggagtgc aagtagctga tgaagtatgt cgcatttttt
      181 atgacatgaa agttcgtaaa tgctccacac cagaagaaat caagaaaaga aagaaggctg
      241 tcattttttg tctcagtgca gacaaaaagt gcatcattgt agaagaaggc aaagagatct
      301 tggttggaga tgttggtgta accataactg atcctttcaa gcattttgtg ggaatgcttc
      361 ctgaaaaaga ttgtcgctat gctttgtatg atgcaagctt tgaaacaaaa gaatccagaa
      421 aagaagagtt gatgtttttt ttgtgggcac cagaactagc acctctgaaa agtaaaatga
      481 tctatgcaag ctccaaggat gcaattaaaa agaaatttca aggcataaaa catgaatgtc
      541 aagcaaatgg accagaagat ctcaatcggg cttgtattgc tgaaaagtta ggtggatcct
      601 taattgtagc ctttgaagga tgccctgtgt agattattca gtgccacaaa ttgaaagctt
      661 ccatgtttaa tgttatcctc ttgctatata aataaagcaa atatatttag gccagggtct
      721 cactgagggg gagctgtctt gtcatctttt agagtaaact attctataaa catatgcaaa
      781 cagccctaaa taaatctaaa gtctaaagtt ttattgatgt gaaattaaat tcttattggc
      841 caaatgcctg ttttgatgag ttgatttata aagatttttg ttaagctcag gattttaaat
      901 tacacagttc acaaacagta aaggccatgt gaagagaatt attacatctt tattaacctc
      961 agcatttact ttgtttcttt tgcttaggaa attgctcata atctggttat aattttggtc
     1021 caaattcttt attcttcctt gagctaagca gaataatgga atataatatg tcttcataat
     1081 ataacaacac taatacacta atagtaagat taagttaggc agtcttctac caaatgtgta
     1141 atggagattg cctcaaaatt gtgtccacat aatccacgct catcttgcaa agcgctattt
     1201 caggcacatc attggaatac aggaagtagc cctgcacctg ccagtgagct cgccattcac
     1261 tgattggaag agtgacctgg catcttggaa atcattgtgt gtcttcagga gaatgtgcag
     1321 tgtcttgtaa caactaatta taatgcaaat tagggctaca ttgtaatctg ctttgttaat
     1381 gaaaatgata aaacagaata ttgacaagct aggacacctg tggtatcttt aattgtatct
     1441 ccttcagaag tttgcttctt atggtataat aaagtatgga agaatattga gtatatgttt
     1501 actctgggcc tgggagaact taactttcta gagcagtttg ttgacttgtg tgcaatgggg
     1561 agaggtacca tgatgacact cacagggagc cactgttcac tgacacttgg aagcggtcat
     1621 tgttaatatc acgggcgtaa cactttgaac gatatagaga tgcacaaaca gttgaactta
     1681 gaagtagcag tattggcttt atgtaataaa ggaagcattt ttgacttacc tctcgtgtaa
     1741 ttttcatgtg tgaattggga atacaggatt ttgccttcca tatggggtaa ggcattgagg
     1801 tttgggtatt tccaataatt aagagttaag gttggctgag tgtggtggct cactcctgta
     1861 atcccagcat tttgggaggc cgaggcaaga ggatagtttg aggccaggag tttgacaatc
     1921 agcgtgggca acatagtggg atctcatcta ctaaaaataa acaaaattag ccgggtgtgg
     1981 tggtgcacgt cagtagtccc agctactttg gatgctgagg tgggaggatc acttgagccc
     2041 aggaggtgag ctgagatcgt gccactgtac tccagcctgg atgacagagc aagaccttgt
     2101 ctcaaaaaaa aaaaaaaaag tcacaactcc ctctggtgaa taagaaagtt ggtgtaaaag
     2161 ttgggaacgt tttcaatgtt tattacacta taagtgtaat aaatattata caaaattata
     2221 aattcacatt taaaatttag aaaatagaga aaagggaaat tatacattgt cctaccacat
     2281 gccatcacat ggttagcatt ttgatacaca gctgtgcatt attacatgtc aaagactgca
     2341 tatactatgg tggtcccata caattataat gtattttcta ctgtaattag atacacaaat
     2401 accattgtgt tacagttgcc gacagtattc agtatagtaa cgtgctgtcc aggtttgtgg
     2461 tctaggagta ataggctcta ccatacagcc taggtgtgta gtaggctatg ccactaggtt
     2521 taagtgcagt ctagggtggg gaacatttta atgttgatcc acatgccagg tgtttgtgag
     2581 acaagattat gctttaatca aaatattttg gaattgtcta gaaaatagtg ctggctgctt
     2641 gtcattcatc ctgaattgct tgtttcttta ctttgtagca atgcttttag tgtgggtttt
     2701 gttttttgag acagggtctc actctttccc ccaggctaga gtgcagtggt gcaatcataa
     2761 ctcactgcat ccttgaactc ttgggctcca gcaagcctcc tgccttggcc tcctaaagtg
     2821 ctgtgattac aggtgtgagc cactgtgtcc tgctagtgat tgtgattaac aggcctccag
     2881 ggaagtgcca taagcccaag actgccgtac tcaaaagtca gccatgtagg taaagcaaga
     2941 tatttggtca aggagtttta gaaggagatt tttcctgtgg tgttggctta agtgtggcac
     3001 tgttgtgcgt gtgtgaggtg tcttctgtgg gaaggatttc aaacagtaca aagggatgtg
     3061 atgtaagcat ccagaactca ctccagaaat tgaataaaat gctagtagtt agtctctagg
     3121 ggaacttttg agaaagtctc agtggattcc tgaggcccaa gaattgtgct aacaagcact
     3181 caaggcgatt ctggtacata attagggttc agacctcagc tgattggtct actgttggtc
     3241 aggaacaaaa ctgcaagtat actctgtttc cagaaaatac gtttacctct tcacaaatgc
     3301 taacacactg caaaaatatg tttgtggaaa gctgacttag tctcatctag cggttactct
     3361 cttgccctga caaagcagca aataaagcca ttgctaacaa ttcaa
//
