LOCUS       NM_001145315            2181 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DSN1 component of MIS12 kinetochore complex (DSN1),
            transcript variant 2, mRNA.
ACCESSION   NM_001145315
VERSION     NM_001145315.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Wang,X., Lu,X., Wang,P., Chen,Q., Xiong,L., Tang,M., Hong,C.,
            Lin,X., Shi,K., Liang,L. and Lin,J.
  TITLE     SRSF9 promotes colorectal cancer progression via stabilizing DSN1
            mRNA in an m6A-related manner
  JOURNAL   J Transl Med 20 (1), 198 (2022)
   PUBMED   35509101
  REMARK    GeneRIF: SRSF9 promotes colorectal cancer progression via
            stabilizing DSN1 mRNA in an m6A-related manner.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2181)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2181)
  AUTHORS   Peng,Q., Wen,T., Liu,D., Wang,S., Jiang,X., Zhao,S. and Huang,G.
  TITLE     DSN1 is a prognostic biomarker and correlated with clinical
            characterize in breast cancer
  JOURNAL   Int Immunopharmacol 101 (Pt B), 107605 (2021)
   PUBMED   34238686
  REMARK    GeneRIF: DSN1 is a prognostic biomarker and correlated with
            clinical characterize in breast cancer.
REFERENCE   5  (bases 1 to 2181)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2181)
  AUTHORS   Kiyomitsu,T., Obuse,C. and Yanagida,M.
  TITLE     Human Blinkin/AF15q14 is required for chromosome alignment and the
            mitotic checkpoint through direct interaction with Bub1 and BubR1
  JOURNAL   Dev Cell 13 (5), 663-676 (2007)
   PUBMED   17981135
REFERENCE   7  (bases 1 to 2181)
  AUTHORS   Kline,S.L., Cheeseman,I.M., Hori,T., Fukagawa,T. and Desai,A.
  TITLE     The human Mis12 complex is required for kinetochore assembly and
            proper chromosome segregation
  JOURNAL   J Cell Biol 173 (1), 9-17 (2006)
   PUBMED   16585270
REFERENCE   8  (bases 1 to 2181)
  AUTHORS   Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R.
  TITLE     Phosphoproteome analysis of the human mitotic spindle
  JOURNAL   Proc Natl Acad Sci U S A 103 (14), 5391-5396 (2006)
   PUBMED   16565220
REFERENCE   9  (bases 1 to 2181)
  AUTHORS   Obuse,C., Iwasaki,O., Kiyomitsu,T., Goshima,G., Toyoda,Y. and
            Yanagida,M.
  TITLE     A conserved Mis12 centromere complex is linked to heterochromatic
            HP1 and outer kinetochore protein Zwint-1
  JOURNAL   Nat Cell Biol 6 (11), 1135-1141 (2004)
   PUBMED   15502821
REFERENCE   10 (bases 1 to 2181)
  AUTHORS   Cheeseman,I.M., Niessen,S., Anderson,S., Hyndman,F., Yates,J.R.
            3rd, Oegema,K. and Desai,A.
  TITLE     A conserved protein network controls assembly of the outer
            kinetochore and its ability to sustain tension
  JOURNAL   Genes Dev 18 (18), 2255-2268 (2004)
   PUBMED   15371340
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC058899.1 and AL132768.15.
            
            On Jun 2, 2019 this sequence version replaced NM_001145315.1.
            
            Summary: This gene encodes a kinetochore protein that functions as
            part of the minichromosome instability-12 centromere complex. The
            encoded protein is required for proper kinetochore assembly and
            progression through the cell cycle. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2009].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058899.1, SRR14243140.6546861.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373750.9/ ENSP00000362855.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1745              BC058899.1         23-1767
            1746-2181           AL132768.15        50905-51340         c
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..2181
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /note="DSN1 component of MIS12 kinetochore complex"
                     /db_xref="GeneID:79980"
                     /db_xref="HGNC:HGNC:16165"
                     /db_xref="MIM:609175"
     exon            1..102
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            103..151
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1188
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /note="isoform 1 is encoded by transcript variant 2;
                     kinetochore null 3 homolog; kinetochore-associated protein
                     DSN1 homolog; DSN1, MIND kinetochore complex component,
                     homolog; DSN1, MIS12 kinetochore complex component; DSN1
                     homolog, MIS12 kinetochore complex component"
                     /codon_start=1
                     /product="kinetochore-associated protein DSN1 homolog
                     isoform 1"
                     /protein_id="NP_001138787.1"
                     /db_xref="CCDS:CCDS13286.1"
                     /db_xref="GeneID:79980"
                     /db_xref="HGNC:HGNC:16165"
                     /db_xref="MIM:609175"
                     /translation="MTSVTRSEIIDEKGPVMSKTHDHQLESSLSPVEVFAKTSASLEM
                     NQGVSEERIHLGSSPKKGGNCDLSHQERLQSKSLHLSPQEQSASYQDRRQSWRRASMK
                     ETNRRKSLHPIHQGITELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFS
                     LESFRAKASSLSEELKHFADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKF
                     SLERQTWDQLLLHYQQEAKEILSRGSTEAKITEVKVEPMTYLGSSQNEVLNTKPDYQK
                     ILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVSVQLGKRSMQQLDPSPAR
                     KLLKLQLQNPPAIHGSGSGSCQ"
     exon            152..472
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            473..546
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            547..619
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            620..707
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            708..767
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            768..842
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            843..990
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1078
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..2181
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1918
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
     regulatory      2158..2163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /note="hexamer: ATTAAA"
     polyA_site      2181
                     /gene="DSN1"
                     /gene_synonym="C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13"
                     /note="major polyA site"
ORIGIN      
        1 ggagaccgct tcaccctgat cagggagtat cggctgcggg tgcgcaaggc gtccaggagt
       61 gacctggggc tgtggagagc gacccgtggc cttgtgtttc agagtttacc acctaggatg
      121 acttcagtga ctagatcaga gatcatagat gaaaaaggac cagtgatgtc taagactcat
      181 gatcatcaat tggaatcaag tctcagtcct gtggaagtgt ttgctaaaac atctgcctcc
      241 ctggagatga atcaaggcgt ttcagaggaa agaattcacc ttggctctag ccctaaaaaa
      301 gggggaaatt gtgatctcag ccaccaggaa agacttcagt cgaagtccct tcatttgtct
      361 cctcaagaac aatctgccag ttatcaagac aggaggcaat cctggcggcg agcaagtatg
      421 aaagaaacga accggcggaa gtcgctgcat cccattcacc agggcatcac agagctcagc
      481 cggtctatca gtgtcgattt agcagaaagc aaacggcttg gctgtctcct gctttccagt
      541 ttccagttct ctattcagaa acttgaacct ttcctaaggg acactaaggg cttcagtctt
      601 gaaagtttta gagccaaagc atcttctctt tctgaagaat tgaaacattt tgcagacgga
      661 ctggaaactg atggaactct acaaaaatgt tttgaagatt caaatggaaa agcatcagat
      721 ttttctttgg aagcatctgt ggctgagatg aaggaataca taacaaagtt ttctttagaa
      781 cgtcagactt gggatcagct cttgcttcac taccagcagg aggctaaaga gatattgtcc
      841 agaggatcaa ctgaggccaa aattactgag gtcaaagtgg aacctatgac atatcttggg
      901 tcttctcaga atgaagttct taatacaaaa cctgactacc agaaaatatt acagaaccag
      961 agcaaagtct ttgactgtat ggagttggtg atggatgaac tgcaaggatc agtgaaacag
     1021 ctgcaggcct ttatggatga aagtacccag tgcttccaga aggtgtcagt acagctcgga
     1081 aagagaagca tgcaacaatt agatccctca ccagctcgaa aactgttgaa gcttcagcta
     1141 cagaacccac ctgccataca tggatctgga tctggatctt gtcagtgact ttatgagagt
     1201 ttctgccaca aggtgcccaa gaggagagga atgggaagag tgccccagca cgtggtgact
     1261 gcgtgatttc tgctcgttgc ctttgaagat aactggcagg actgactgta gaacactttg
     1321 acttttttca aaaagtgatg gaatttgtac atccaaatga atattgtata gacaattttc
     1381 ccaggaatgt gcaaaatgct tgaaagttca aacttctttt ttgaaatgat cttcagatcc
     1441 agtggcccat tcttttatct ttatcctgtg aaggtgtttt tcaggttttg aaacaatcca
     1501 aaaatcattt aggaccaagt ctaaggaaac attttagtgg ccaagttgga ttccgattgt
     1561 aaaggaatga tactaatttt ctagcatggc tctgaaggtg attttaggta gaagagtttt
     1621 gaggctgggc gcaatggctc acgcctgtaa tcctagcatt ttgggtgact gaggcgggtg
     1681 gattgcttga gcccagaagt tgaagaccag cctgagaaat aaggtgaaac cctgtctaca
     1741 aaaaatacaa aaagttagct gggtgtggtg gcgtgtgcct gtagtgctag ctactcagaa
     1801 ggctgaggtg ggaggattgt ttgagcccag gaggttgagg ctgcagtgag ttctaattgc
     1861 gccactgcac tccagcctga gcgacagagt gagacactgt cttaaaaaaa attaaaaatt
     1921 gtaaaaaaat gaaaaaaaaa gttttgagca ttatttgcat cattgggata catatgtcac
     1981 ttcacaagat gttcaatttg aaggaaatac cactcattct ctatgtcctg ttgtctgtag
     2041 tgtgcttcag tttttcatat tgagttgacc taaatcctgg attcatgaca agaaaggagt
     2101 aagtactact attcattgtt ctatttgttt ataatctgta ttataaaatt gcacataatt
     2161 aaaagctttc ccttgtcttc a
//
