LOCUS       NM_001942               7191 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens desmoglein 1 (DSG1), mRNA.
ACCESSION   NM_001942
VERSION     NM_001942.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Burks,H.E., Pokorny,J.L., Koetsier,J.L., Roth-Carter,Q.R.,
            Arnette,C.R., Gerami,P., Seykora,J.T., Johnson,J.L., Ren,Z. and
            Green,K.J.
  TITLE     Melanoma cells repress Desmoglein 1 in keratinocytes to promote
            tumor cell migration
  JOURNAL   J Cell Biol 222 (11) (2023)
   PUBMED   37733372
  REMARK    GeneRIF: Melanoma cells repress Desmoglein 1 in keratinocytes to
            promote tumor cell migration.
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Kawamura,T., Muramatsu,K., Orita,A., Mai,Y., Sugai,T., Haga,N.,
            Fujimura,Y., Miyauchi,T., Izumi,K., Koga,H., Ishii,N. and Ujiie,H.
  TITLE     Two cases of Hallopeau-type pemphigus vegetans with anti-desmoglein
            1 and anti-desmocollin 3 antibodies without mucosal involvement
  JOURNAL   J Eur Acad Dermatol Venereol 37 (4), e508-e510 (2023)
   PUBMED   36305887
  REMARK    GeneRIF: Two cases of Hallopeau-type pemphigus vegetans with
            anti-desmoglein 1 and anti-desmocollin 3 antibodies without mucosal
            involvement.
REFERENCE   3  (bases 1 to 7191)
  AUTHORS   Hegazy,M., Koetsier,J.L., Huffine,A.L., Broussard,J.A.,
            Godsel,B.M., Cohen-Barak,E., Sprecher,E., Wolfgeher,D.J.,
            Kron,S.J., Godsel,L.M. and Green,K.J.
  TITLE     Epidermal stratification requires retromer-mediated desmoglein-1
            recycling
  JOURNAL   Dev Cell 57 (24), 2683-2698 (2022)
   PUBMED   36495876
  REMARK    GeneRIF: Epidermal stratification requires retromer-mediated
            desmoglein-1 recycling.
REFERENCE   4  (bases 1 to 7191)
  AUTHORS   Schmitt,T., Pircher,J., Steinert,L., Meier,K., Ghoreschi,K.,
            Vielmuth,F., Kugelmann,D. and Waschke,J.
  TITLE     Dsg1 and Dsg3 Composition of Desmosomes Across Human Epidermis and
            Alterations in Pemphigus Vulgaris Patient Skin
  JOURNAL   Front Immunol 13, 884241 (2022)
   PUBMED   35711465
  REMARK    GeneRIF: Dsg1 and Dsg3 Composition of Desmosomes Across Human
            Epidermis and Alterations in Pemphigus Vulgaris Patient Skin.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7191)
  AUTHORS   Samuelov,L., Sarig,O., Harmon,R.M., Rapaport,D.,
            Ishida-Yamamoto,A., Isakov,O., Koetsier,J.L., Gat,A., Goldberg,I.,
            Bergman,R., Spiegel,R., Eytan,O., Geller,S., Peleg,S., Shomron,N.,
            Goh,C.S.M., Wilson,N.J., Smith,F.J.D., Pohler,E., Simpson,M.A.,
            McLean,W.H.I., Irvine,A.D., Horowitz,M., McGrath,J.A., Green,K.J.
            and Sprecher,E.
  TITLE     Desmoglein 1 deficiency results in severe dermatitis, multiple
            allergies and metabolic wasting
  JOURNAL   Nat Genet 45 (10), 1244-1248 (2013)
   PUBMED   23974871
  REMARK    GeneRIF: DSG1 deficiency was associated with increased expression
            of a number of genes encoding allergy-related cytokines
REFERENCE   6  (bases 1 to 7191)
  AUTHORS   Rickman,L., Simrak,D., Stevens,H.P., Hunt,D.M., King,I.A.,
            Bryant,S.P., Eady,R.A., Leigh,I.M., Arnemann,J., Magee,A.I.,
            Kelsell,D.P. and Buxton,R.S.
  TITLE     N-terminal deletion in a desmosomal cadherin causes the autosomal
            dominant skin disease striate palmoplantar keratoderma
  JOURNAL   Hum Mol Genet 8 (6), 971-976 (1999)
   PUBMED   10332028
REFERENCE   7  (bases 1 to 7191)
  AUTHORS   Amagai,M., Klaus-Kovtun,V. and Stanley,J.R.
  TITLE     Autoantibodies against a novel epithelial cadherin in pemphigus
            vulgaris, a disease of cell adhesion
  JOURNAL   Cell 67 (5), 869-877 (1991)
   PUBMED   1720352
REFERENCE   8  (bases 1 to 7191)
  AUTHORS   Nilles,L.A., Parry,D.A., Powers,E.E., Angst,B.D., Wagner,R.M. and
            Green,K.J.
  TITLE     Structural analysis and expression of human desmoglein: a
            cadherin-like component of the desmosome
  JOURNAL   J Cell Sci 99 (Pt 4), 809-821 (1991)
   PUBMED   1770008
REFERENCE   9  (bases 1 to 7191)
  AUTHORS   Arnemann,J., Spurr,N.K., Wheeler,G.N., Parker,A.E. and Buxton,R.S.
  TITLE     Chromosomal assignment of the human genes coding for the major
            proteins of the desmosome junction, desmoglein DGI (DSG),
            desmocollins DGII/III (DSC), desmoplakins DPI/II (DSP), and
            plakoglobin DPIII (JUP)
  JOURNAL   Genomics 10 (3), 640-645 (1991)
   PUBMED   1889810
REFERENCE   10 (bases 1 to 7191)
  AUTHORS   Wheeler,G.N., Parker,A.E., Thomas,C.L., Ataliotis,P., Poynter,D.,
            Arnemann,J., Rutman,A.J., Pidsley,S.C., Watt,F.M., Rees,D.A. et al.
  TITLE     Desmosomal glycoprotein DGI, a component of intercellular desmosome
            junctions, is related to the cadherin family of cell adhesion
            molecules
  JOURNAL   Proc Natl Acad Sci U S A 88 (11), 4796-4800 (1991)
   PUBMED   1711210
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF097935.1, BX476267.1,
            CU456411.1 and AC009717.15.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001942.3.
            
            Summary: This gene encodes a member of the desmoglein protein
            subfamily. Desmogleins, along with desmocollins, are cadherin-like
            transmembrane glycoproteins that are major components of the
            desmosome. Desmosomes are cell-cell junctions that help resist
            shearing forces and are found in high concentrations in cells
            subject to mechanical stress. This gene is found in a cluster with
            other desmoglein family members on chromosome 18. The encoded
            protein has been identified as a target of auto-antibodies in the
            autoimmune skin blistering disease pemphigus foliaceus. Disruption
            of this gene has also been associated with the skin diseases
            palmoplantar keratoderma and erythroderma. [provided by RefSeq, Feb
            2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF097935.1, X56654.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145122, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257192.5/ ENSP00000257192.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AF097935.1         73-150
            79-172              BX476267.1         123-216
            173-872             AF097935.1         245-944
            873-873             CU456411.1         707-707             c
            874-3356            AF097935.1         946-3428
            3357-7191           AC009717.15        14920-18754         c
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..7191
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="desmoglein 1"
                     /db_xref="GeneID:1828"
                     /db_xref="HGNC:HGNC:3048"
                     /db_xref="MIM:125670"
     exon            1..189
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..3291
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="desmosomal glycoprotein 1; pemphigus foliaceus
                     antigen; cadherin family member 4"
                     /codon_start=1
                     /product="desmoglein-1 preproprotein"
                     /protein_id="NP_001933.2"
                     /db_xref="CCDS:CCDS11896.1"
                     /db_xref="GeneID:1828"
                     /db_xref="HGNC:HGNC:3048"
                     /db_xref="MIM:125670"
                     /translation="MDWSFFRVVAMLFIFLVVVEVNSEFRIQVRDYNTKNGTIKWHSI
                     RRQKREWIKFAAACREGEDNSKRNPIAKIHSDCAANQQVTYRISGVGIDQPPYGIFVI
                     NQKTGEINITSIVDREVTPFFIIYCRALNSMGQDLERPLELRVRVLDINDNPPVFSMA
                     TFAGQIEENSNANTLVMILNATDADEPNNLNSKIAFKIIRQEPSDSPMFIINRNTGEI
                     RTMNNFLDREQYGQYALAVRGSDRDGGADGMSAECECNIKILDVNDNIPYMEQSSYTI
                     EIQENTLNSNLLEIRVIDLDEEFSANWMAVIFFISGNEGNWFEIEMNERTNVGILKVV
                     KPLDYEAMQSLQLSIGVRNKAEFHHSIMSQYKLKASAISVTVLNVIEGPVFRPGSKTY
                     VVTGNMGSNDKVGDFVATDLDTGRPSTTVRYVMGNNPADLLAVDSRTGKLTLKNKVTK
                     EQYNMLGGKYQGTILSIDDNLQRTCTGTININIQSFGNDDRTNTEPNTKITTNTGRQE
                     STSSTNYDTSTTSTDSSQVYSSEPGNGAKDLLSDNVHFGPAGIGLLIMGFLVLGLVPF
                     LMICCDCGGAPRSAAGFEPVPECSDGAIHSWAVEGPQPEPRDITTVIPQIPPDNANII
                     ECIDNSGVYTNEYGGREMQDLGGGERMTGFELTEGVKTSGMPEICQEYSGTLRRNSMR
                     ECREGGLNMNFMESYFCQKAYAYADEDEGRPSNDCLLIYDIEGVGSPAGSVGCCSFIG
                     EDLDDSFLDTLGPKFKKLADISLGKESYPDLDPSWPPQSTEPVCLPQETEPVVSGHPP
                     ISPHFGTTTVISESTYPSGPGVLHPKPILDPLGYGNVTVTESYTTSDTLKPSVHVHDN
                     RPASNVVVTERVVGPISGADLHGMLEMPDLRDGSNVIVTERVIAPSSSLPTSLTIHHP
                     RESSNVVVTERVIQPTSGMIGSLSMHPELANAHNVIVTERVVSGAGVTGISGTTGISG
                     GIGSSGLVGTSMGAGSGALSGAGISGGGIGLSSLGGTASIGHMRSSSDHHFNQTIGSA
                     SPSTARSRITKYSTVQYSK"
     exon            190..225
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            226..357
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            358..513
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..658
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..825
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..960
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1146
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1406
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1546
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1828
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1962
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2032
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2241
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..7191
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4946..4951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="hexamer: AATAAA"
     polyA_site      4963
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="major polyA site"
     regulatory      5229..5234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="hexamer: AATAAA"
     polyA_site      5248
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="major polyA site"
     regulatory      7173..7178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="hexamer: AATAAA"
     polyA_site      7191
                     /gene="DSG1"
                     /gene_synonym="CDHF4; DG1; DSG; EPKHE; EPKHIA; PPKS1;
                     SPPK1"
                     /note="major polyA site"
ORIGIN      
        1 agaaacacct caaagcctgc atgtaagaac atctactgag aaattatttt aatcagacac
       61 cagctgagtg ggagaaagaa aaagaacaga gaagaacaaa caaaactccc ttggtcttgg
      121 atgtaagaga atccagcaga gatggactgg agtttcttca gagtagttgc aatgctgttc
      181 atttttctgg tggtggtaga agttaacagt gaattccgaa tccaggtaag agattataac
      241 actaaaaatg gcaccatcaa atggcattca atccgaaggc agaaacgtga atggatcaag
      301 ttcgcagcag cctgtcgtga aggtgaagac aactcaaaga ggaacccaat cgccaaaatt
      361 cactcagatt gtgctgcaaa ccagcaagtt acataccgca tctctggagt aggaattgat
      421 cagccaccat atgggatctt tgtcattaat cagaaaactg gtgaaattaa tataacatcc
      481 atagttgatc gagaggtcac tcctttcttc attatctact gccgagctct gaactcaatg
      541 ggccaagatt tagagaggcc tctagagctc agagtcaggg ttttggatat aaatgacaac
      601 cctccagtgt tttcaatggc tacatttgca ggacaaatag aagaaaattc taatgcaaat
      661 acactggtga tgatactcaa tgctactgac gcagatgaac cgaacaattt gaactcaaaa
      721 atagccttca agattataag acaagaacct tcagattcac caatgtttat tatcaacaga
      781 aatactggag aaattcgaac gatgaataat tttctagaca gagagcaata cggccagtat
      841 gctcttgctg taagaggctc tgaccgagat ggcggggcag atggcatgtc agcggaatgt
      901 gagtgcaaca ttaaaatcct cgatgtcaat gataatatcc cttacatgga acagtcttca
      961 tataccatag aaattcaaga aaatactcta aattcaaatt tgctcgagat tagagtaatt
     1021 gatttggatg aagagttctc agctaactgg atggcagtaa ttttctttat ctctggaaat
     1081 gaaggaaatt ggtttgagat agaaatgaat gaaagaacaa atgtgggaat tttaaaggtt
     1141 gttaagccct tagattatga agctatgcag agtctgcaac tcagtattgg tgtcagaaat
     1201 aaagctgaat ttcatcattc aattatgtct caatataaac tgaaagcatc tgcaatttct
     1261 gtgactgtgt taaatgtaat tgaaggccca gtgtttcgtc caggttcaaa gacatatgtt
     1321 gtaactggta atatgggatc aaatgataaa gtgggagact ttgtagctac tgacctggac
     1381 acaggtagac cttcaacgac tgttaggtat gtaatgggaa ataatccagc tgacctgcta
     1441 gctgttgatt caagaacagg caaactcact ttgaaaaata aagttaccaa ggaacagtac
     1501 aatatgctcg gaggaaaata ccaaggaacg attctctcta tagatgataa tcttcaaaga
     1561 acttgcactg gtacaattaa tattaacatt caaagttttg gtaatgacga caggactaat
     1621 acagagccga acactaaaat tactaccaat actggcagac aagaaagtac ttcttccact
     1681 aactatgata ccagcacaac ttctactgac tctagccaag tatattcttc tgaacccgga
     1741 aacggagcca aagatttgtt atcagacaat gtacattttg gtcctgctgg cattggactc
     1801 ctcatcatgg gattcttggt cttaggattg gtcccatttt tgatgatctg ttgtgattgt
     1861 ggaggtgctc ctcgtagtgc agctggcttt gagcctgttc ccgaatgttc agatggagca
     1921 attcattcat gggcagtaga aggaccacag cctgaaccca gggatataac cactgtcata
     1981 ccacaaatac cacctgataa cgcaaatata attgaatgca ttgacaactc aggagtttat
     2041 acaaatgagt atggtggcag agaaatgcaa gatctgggag gaggagagag aatgacagga
     2101 tttgaactaa cagagggagt taaaacttca ggaatgcctg agatatgtca agaatactct
     2161 ggaacattaa gaagaaattc tatgagggaa tgtagagaag gaggtctgaa tatgaatttc
     2221 atggaaagct acttctgtca gaaagcatat gcttacgcag atgaagatga aggacgccca
     2281 tctaatgact gtttgctcat atatgacatc gaaggtgtag gttcccctgc tggctctgtg
     2341 ggttgttgta gcttcattgg agaagacctg gatgacagct tcttggatac cctgggacct
     2401 aaatttaaga agttggcaga catcagccta ggaaaagaat catatccaga ccttgatcct
     2461 tcttggccac cacaaagcac tgaaccagtt tgccttcctc aggaaacaga gcccgttgtt
     2521 agtggacacc caccaatctc cccacatttc ggcactacca cagtaatttc tgagagcacc
     2581 tatccctcgg gacctggtgt actgcatcct aagcctattc tcgatcctct gggctatggt
     2641 aatgtcactg tgaccgagtc ttacaccacc tctgacactc tgaagccctc tgtgcacgtt
     2701 cacgataacc gaccagcatc aaacgtggta gtgacagaga gagtggtcgg cccaatctct
     2761 ggcgctgatt tgcatggaat gttagagatg cctgacttgc gagatgggtc gaatgttata
     2821 gtgacagaaa gggtaatagc accaagctct agtctaccca cctctctgac tatccatcat
     2881 cctagagagt cttcaaatgt ggtagtgaca gaaagagtaa tccaaccaac ttccggcatg
     2941 ataggtagtc tgagtatgca ccccgagtta gccaatgccc acaatgtcat tgtgacagag
     3001 agggttgttt ctggtgctgg cgtaactgga attagtggca ccactgggat cagcggtggc
     3061 ataggcagca gtggcctggt tggcaccagc atgggtgctg ggagcggtgc cctgagtgga
     3121 gctggcataa gtggtggtgg cattggcctg agcagcttgg gagggacagc cagcattggc
     3181 cacatgagga gttcctctga ccatcacttt aaccaaacca ttgggtccgc ctcccctagc
     3241 acagctcgaa gtcgaatcac aaagtatagt accgtgcaat atagcaagta gtcaggaccc
     3301 cagctcactt tttcatagtc attgtggttt agatccaatt cccaccacta aaaaaccaac
     3361 aatgtgattt ataacgcaca acttcgtgct caggtcatct aggagcaagg tgagaaatca
     3421 caatgagaaa aataaatgga aacaccactg ctaggggaga gctctcctta gcattcataa
     3481 acttttctct tatattagga ctaaggaact aaaacttgag gcagagtctt ctttgtgcct
     3541 gagtggcctg tagtccatct ccagcatgta actggcctta cgatggcaat tggcatcatt
     3601 ctccttgctc tgttttgctt ttccatatag ctcgagcaaa attcaaaaag aactaaatat
     3661 gcaatatatg ttcatatcta tgggaaaaat ctaaaatgtg tgccagatgc cctgttggtt
     3721 tcacagataa cataaataaa aattcaacca cagatttata caagggttaa ccattttttt
     3781 taagtttgac tacatagtca agtccacaag ccatcaagca ctcctacctt aattattgca
     3841 ctagagaaaa taaattccaa attaggaagt gtttcctagg aggaaaattc cattagagag
     3901 tggcaatagg atgaggtttc ttcagggtaa actagcaatg cctgagcctg aaccttaatg
     3961 tggggcctca gttaaatctc ctgtggagtc aaggattctt ctgattctag tgtgtgttta
     4021 gtgatagatg tagtcttgac gaatattgct tactggtgag gttgaggaat atcacactcg
     4081 tctttccctt taccactgtg gttttgactt aagaaagcaa aactcactaa gtttacttct
     4141 cgaattgaag caagtgaggc ctgacatggt tgtcatcact agtggcaaat gaccttccaa
     4201 gtaagcagat gggaactgaa ttgtgttttc aggttttgtt tttagtaggt gatattcatt
     4261 cgtatccagc tctttattac atagctctga agttaaaatg atttacatag gccgagctgt
     4321 ggacaaaaaa aaaagaagca gcagcttgta gtatgcttaa gctttgggga attttttttt
     4381 aaggggatct aaaaaaatgt ttttagaaca tgtaaaatgt ttaatggtga aagttggaaa
     4441 agaattcttc tgtaaagtaa taccatgcta attattcgct tttagtaagt aaagtagtgg
     4501 ttgctttagc aaacctctgc tgccattttg caggaatcaa ccaggaacct ttagcagaat
     4561 tgacaatatg gtgttgataa gcatgaaata ataatagaaa cctattctgc tagtttatct
     4621 caccctctaa tttttctcac tagcataaat tttaaattcc tgatttgatt tgtcaataag
     4681 atcttggctt atatatgctg atttataggt agtgtccaaa ttatatagta taacatattt
     4741 ttctagtttc aaaatttagt aatgtcctat ttatgatata tcatttctgt gtgtttgcta
     4801 tgtagtatta cccaattaaa aatctctaaa aagaattaaa gcattctaag aaaaaggtaa
     4861 atttactatt gcatggtaca gaaatttttt ctttcttaaa tacaatgtta ctataagctc
     4921 actaaaatga aactctatat gacaaaataa aattagaaaa aaatttgccc tggagttgtg
     4981 aattatatac aacttttaaa gaatttaccc caattactca aatttcccag gaaattacaa
     5041 agccaaagaa tattcaactt cctccactgg tcaaaagagg ataggagtga attactgaac
     5101 ctagagctat tttgctctgt aacaacagat aaggctaata ttttaaaagc cacagtatac
     5161 atcttctttt aactctgtag aatatgtaaa attttgatag tctgtagtat gctaaatgca
     5221 gaagtataaa taaagtcatt caaagggagt cttttctttt tctgacactt agggggccac
     5281 attaaggatg ggtaatcttt ccaggaataa agtcaaaagg tatttattaa gacatacttg
     5341 agtatgcctg ggtccaggag ttttaaggaa tagaaataag tattaatgaa ttaattaagt
     5401 aatttattaa agggaatggt agctgaccac aggaaacttg cttactgttt tgatatgaaa
     5461 tatcatcaca agcttttctt aagacatctg atatcttcca gagatatttt ttaggttgtc
     5521 ttgcaaacaa caaaatcact gtctttaata actgttgctg tcaaaatcca ttggttgtta
     5581 agatcccccc aatttagtta catctgaact cctaaacact gttaaacgat gggaaaaaca
     5641 agaaaaaaca tggccatttg agtcattgag tcatctatct ttctaggaag atactttcta
     5701 accaaacttt tcttccagga ttgcaaattg atgggaaaaa caagaaaaac tgaagtatta
     5761 gtcacctatc tttctgggaa gatactttcc aactaatttt ttcttccagg attgcacact
     5821 gattttccat ttagtcctaa attttaaaat tcccttttca agacatcaac gattttagta
     5881 gttatttaaa ggcatgtcat ttttcaatga agaagttttg ggcagaactt cattcttctt
     5941 cttagatgtt tactctagat catatacatc atgtcataga ccaagaagag atatggaaat
     6001 tattttataa gtgaatacta taattaggat tcaagctgag tttcagatca acttgctctt
     6061 aacaaaagga aaaagaaata gtaatttaat actatgtatg tatggtttga aaacaaacca
     6121 caatgtttat aaaatatcta tctgactgtc taaagaggta atctttagga gcaaaaatca
     6181 gtgtattata aatactttac catttaatat caaccaaaat accatctcaa gctaattttg
     6241 acactgaatt acagatatat ctgctacata ttatttactt ctaagcatgt tgtctgatgt
     6301 aattgcattt gcactgaaaa attaaaagaa aaagtacata tttagggtta tttatatatc
     6361 ttcatctaga catctgttct acatttgtgt ataaagtttt tagcatcata atttttattc
     6421 aagaaaatgt tctgacaaaa ttttaattat atgtcttcaa aaattacatt ttttactcta
     6481 gtaagtagat gtttttagtt atctggcaat ttatttctga atttatacca atgtttgatt
     6541 gtcatggtac aaaatatatg acacccttta acttttgctg gagttgaaag gcattataat
     6601 ctttagcata aatggccatg actattttgg aaagacattt aagacccaaa gcaaactttt
     6661 aaaagtattt gccacatttt cccatgccta tttcataaat tccaactttt ttttttacaa
     6721 tttctggatt tttaagaccc atttcacatt gcactaggat acagcagtcc acagtagagt
     6781 gctactctcc ttgaaatcaa atctgtcttc cacttccgga ttattcaatt tatgttagga
     6841 caaatcttga ctagatcaac ctgttttcca tcagataatt ttaaaacaat gtgtaatctt
     6901 gtttgtctac attctctccc cagtttagct gtatttgaat tactaaatgc tttatcgtca
     6961 aactgtacct agtctaactt atttttcttt tgctgtcgtt ttacaagcat tttaaaattc
     7021 taatattcat ctctggtggt gtttaacaca aggttctctt attcaagttt caatataaaa
     7081 gtttttggat tatttgggtg ctagtttctt gcttggttat ctgttcgttt ttttaagttg
     7141 atttgtaatt tccaaagagt tatgcataca gcaataaaat tattaatatg c
//
