LOCUS       NM_001389               8571 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens DS cell adhesion molecule (DSCAM), transcript variant
            1, mRNA.
ACCESSION   NM_001389
VERSION     NM_001389.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   McKean,D.M., Zhang,Q., Narayan,P., Morton,S.U., Strohmenger,V.,
            Tang,V.T., McAllister,S., Sharma,A., Quiat,D., Reichart,D.,
            DeLaughter,D.M., Wakimoto,H., Gorham,J.M., Brown,K., McDonough,B.,
            Willcox,J.A., Jang,M.Y., DePalma,S.R., Ward,T., Kim,R.,
            Cleveland,J.D., Seidman,J.G. and Seidman,C.E.
  CONSRTM   Pediatric Cardiac Genomics Consortium Investigators
  TITLE     Increased endothelial sclerostin caused by elevated DSCAM mediates
            multiple trisomy 21 phenotypes
  JOURNAL   J Clin Invest 134 (11), e167811 (2024)
   PUBMED   38828726
  REMARK    GeneRIF: Increased endothelial sclerostin caused by elevated DSCAM
            mediates multiple trisomy 21 phenotypes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8571)
  AUTHORS   Wang,N., Yang,Y., Jia,G.Z., Wang,K., Zhou,S., Zhang,B., Zhang,Z.S.,
            Qiao,Q. and He,X.L.
  TITLE     Long non-coding RNA Down syndrome cell adhesion molecule-anti-sense
            1 promotes gastric carcinoma cell proliferation and migration by
            regulating the miR-204/TPT1 axis
  JOURNAL   Hum Exp Toxicol 40 (12_suppl), S187-S195 (2021)
   PUBMED   34372727
  REMARK    GeneRIF: Long non-coding RNA Down syndrome cell adhesion
            molecule-anti-sense 1 promotes gastric carcinoma cell proliferation
            and migration by regulating the miR-204/TPT1 axis.
REFERENCE   4  (bases 1 to 8571)
  AUTHORS   Lim,C.S., Kim,M.J., Choi,J.E., Islam,M.A., Lee,Y.K., Xiong,Y.,
            Shim,K.W., Yang,J.E., Lee,R.U., Lee,J., Park,P., Kwak,J.H., Seo,H.,
            Kim,C.H., Lee,J.H., Lee,Y.S., Hwang,S.K., Lee,K., Lee,J.A. and
            Kaang,B.K.
  TITLE     Dysfunction of NMDA receptors in neuronal models of an autism
            spectrum disorder patient with a DSCAM mutation and in
            Dscam-knockout mice
  JOURNAL   Mol Psychiatry 26 (12), 7538-7549 (2021)
   PUBMED   34253863
  REMARK    GeneRIF: Dysfunction of NMDA receptors in neuronal models of an
            autism spectrum disorder patient with a DSCAM mutation and in
            Dscam-knockout mice.
REFERENCE   5  (bases 1 to 8571)
  AUTHORS   Lemieux,M., Thiry,L., Laflamme,O.D. and Bretzner,F.
  TITLE     Role of DSCAM in the Development of Neural Control of Movement and
            Locomotion
  JOURNAL   Int J Mol Sci 22 (16), 8511 (2021)
   PUBMED   34445216
  REMARK    GeneRIF: Role of DSCAM in the Development of Neural Control of
            Movement and Locomotion.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8571)
  AUTHORS   Meijers,R., Puettmann-Holgado,R., Skiniotis,G., Liu,J.H., Walz,T.,
            Wang,J.H. and Schmucker,D.
  TITLE     Structural basis of Dscam isoform specificity
  JOURNAL   Nature 449 (7161), 487-491 (2007)
   PUBMED   17721508
  REMARK    GeneRIF: The specificity of Drosophila Dscam is due to
            complementarity of variable residues in epitope I.
REFERENCE   7  (bases 1 to 8571)
  AUTHORS   Barlow,G.M., Lyons,G.E., Richardson,J.A., Sarnat,H.B. and
            Korenberg,J.R.
  TITLE     DSCAM: an endogenous promoter drives expression in the developing
            CNS and neural crest
  JOURNAL   Biochem Biophys Res Commun 299 (1), 1-6 (2002)
   PUBMED   12435380
  REMARK    GeneRIF: A specific promoter region directs expression of DSCAM in
            the developing choroid plexus and roof of the fourth ventricle, the
            floor plate of the fourth ventricle, pons and medulla oblongata,
            and the eye, limb buds, and dorsal root ganglion
REFERENCE   8  (bases 1 to 8571)
  AUTHORS   Barlow,G.M., Micales,B., Chen,X.N., Lyons,G.E. and Korenberg,J.R.
  TITLE     Mammalian DSCAMs: roles in the development of the spinal cord,
            cortex, and cerebellum?
  JOURNAL   Biochem Biophys Res Commun 293 (3), 881-891 (2002)
   PUBMED   12051741
REFERENCE   9  (bases 1 to 8571)
  AUTHORS   Agarwala,K.L., Nakamura,S., Tsutsumi,Y. and Yamakawa,K.
  TITLE     Down syndrome cell adhesion molecule DSCAM mediates homophilic
            intercellular adhesion
  JOURNAL   Brain Res Mol Brain Res 79 (1-2), 118-126 (2000)
   PUBMED   10925149
REFERENCE   10 (bases 1 to 8571)
  AUTHORS   Yamakawa,K., Huot,Y.K., Haendelt,M.A., Hubert,R., Chen,X.N.,
            Lyons,G.E. and Korenberg,J.R.
  TITLE     DSCAM: a novel member of the immunoglobulin superfamily maps in a
            Down syndrome region and is involved in the development of the
            nervous system
  JOURNAL   Hum Mol Genet 7 (2), 227-237 (1998)
   PUBMED   9426258
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF043945.2, AF217525.1,
            BE503065.1, AF064862.2 and BM684380.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001389.4.
            
            Summary: This gene is a member of the immunoglobulin superfamily of
            cell adhesion molecules (Ig-CAMs), and is involved in human central
            and peripheral nervous system development. This gene is a candidate
            for Down syndrome and congenital heart disease (DSCHD). A gene
            encoding a similar Ig-CAM protein is located on chromosome 11.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Oct
            2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (CHD2-42).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF217525.1, AB209136.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA1968968 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000400454.6/ ENSP00000383303.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AF043945.2         55710-55728         c
            20-6660             AF217525.1         1-6641
            6661-7154           BE503065.1         7-500               c
            7155-8503           AF064862.2         21147-22495         c
            8504-8571           BM684380.1         18-85               c
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.2"
     gene            1..8571
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /note="DS cell adhesion molecule"
                     /db_xref="GeneID:1826"
                     /db_xref="HGNC:HGNC:3039"
                     /db_xref="MIM:602523"
     exon            1..540
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     CDS             498..6536
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /note="isoform CHD2-42 precursor is encoded by transcript
                     variant 1; Down syndrome cell adhesion molecule; cell
                     adhesion molecule DSCAM"
                     /codon_start=1
                     /product="cell adhesion molecule DSCAM isoform CHD2-42
                     precursor"
                     /protein_id="NP_001380.2"
                     /db_xref="CCDS:CCDS42929.1"
                     /db_xref="GeneID:1826"
                     /db_xref="HGNC:HGNC:3039"
                     /db_xref="MIM:602523"
                     /translation="MWILALSLFQSFANVFSEDLHSSLYFVNASLQEVVFASTTGTLV
                     PCPAAGIPPVTLRWYLATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYC
                     TAENPSGKIRSQDVHIKAVLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSW
                     EKDTVSLVSGSRFLITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVS
                     DPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVT
                     GLLIENIRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLS
                     CSVTGTEDQELSWYRNGEILNPGKNVRITGINHENLIMDHMVKSDGGAYQCFVRKDKL
                     SAQDYVQVVLEDGTPKIISAFSEKVVSPAEPVSLMCNVKGTPLPTITWTLDDDPILKG
                     GSHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSAGVVLYQARINVRGPASIR
                     PMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNSNLLPFNHRQVAFENNGTLKLSDVQK
                     EVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPFEFPRFSIGQRVFIPCVVVSGDL
                     PITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQS
                     QLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALN
                     GRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSMYLTVKIPAMITSYPNTTL
                     ATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTKEVGEEVISTLQILPTV
                     REDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFDGNSP
                     ITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPS
                     NELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTG
                     GNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVP
                     SYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEIKNITTT
                     QPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAASASM
                     VFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVWVV
                     AVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWM
                     KDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQV
                     PPDQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSY
                     RLENLKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRL
                     NLIGWNDGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCN
                     SAGCAEKQANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLVTISCILVGVLLLF
                     VLLLVVRRRRREQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLL
                     IEERDTMETIDDRSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARP
                     GTNPTTRRNAKAGPTARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESD
                     SYSVSPSQDTDRARSSMVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDT
                     SSEQLTAGTNEYTDSLTSSTPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIH
                     LPPYLRMDFLLNRGGPGTSRDLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTR
                     EGQSWQPGAVATLPQREGAELGQAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV"
     exon            541..858
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1005
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1152
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1431
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1707
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..2004
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2280
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2559
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2679
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2853
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..3050
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3147
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3148..3276
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3444
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3515
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3516..3756
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3757..3903
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            3904..4059
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4060..4193
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4194..4347
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4348..4465
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4466..4629
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4630..4728
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4729..4917
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            4918..5208
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5209..5385
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5386..5416
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5417..5532
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5533..5682
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5683..5880
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            5881..6183
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     exon            6184..8571
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7129..7134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /note="hexamer: AATAAA"
     polyA_site      7157
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /note="major polyA site"
     regulatory      8552..8557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
                     /note="hexamer: AATAAA"
     polyA_site      8571
                     /gene="DSCAM"
                     /gene_synonym="CHD2; CHD2-42; CHD2-52"
ORIGIN      
        1 actcgcccgg ctcgcaggca gggaaggttg gcagagggat ccatgtgact gaggccggag
       61 cacggcaaag atgagcctgc ccgcccgcct gctgcctgga tgcggagggt gagggctggc
      121 gcacgggagg ccgctggctg cgcattctgg gcgccgagtg cccgggatga gctcacgccc
      181 gcgtctgcgg ctctctccac ctgccgacct gccgggggcc cactgagctg acggcgcacc
      241 tgggctccgg ccgcagcgtg gggcgcggcg cccgggagca ggtgtgcagg agcgcagcgc
      301 gcggcgagcg cagccctcgc tccggagccc ggccgcgccg cgtgcccggg cggctaggca
      361 gcggcggcgg cggcggcggg cggcgggcgg gcggcggccc ccgggcaggt gccgagcggc
      421 gagcggagcc gggccgggcg gagcgcgggg ggcgaggccg gcgcgtcgct cgcgggaggc
      481 cggggagcgg caggggcatg tggatactgg ctctctcctt gttccagagc ttcgcgaatg
      541 ttttcagtga agacctacac tccagcctct actttgtcaa tgcatctctg caagaggtag
      601 tgtttgccag caccacgggg actctggtgc cctgccccgc agcaggcatc cctcctgtga
      661 ctctcagatg gtacctagcc acgggcgagg agatctacga tgtccccggg atccgccacg
      721 tccaccccaa cggcactctc caaattttcc ccttccctcc ttcaagcttc agtaccttaa
      781 tccatgataa tacttattat tgcacagctg aaaatccttc agggaaaatt agaagtcagg
      841 atgtccacat caaggctgtt ttacgggagc cctatacagt ccgtgtggag gaccagaaaa
      901 ccatgagagg caatgttgcg gtcttcaagt gcattatccc ctcctcggtg gaggcgtaca
      961 tcactgtcgt ctcatgggag aaagacactg tttcacttgt ctcaggatct agatttctca
     1021 tcacatccac gggagccttg tatattaaag atgtacagaa tgaagatgga ttgtataact
     1081 accgctgcat cacgcggcat cgatacaccg gagagacgag gcagagcaac agcgccagac
     1141 tttttgtatc agacccagcg aactcagccc catccatact ggatgggttt gaccatcgca
     1201 aagccatggc tgggcagcgt gtggagctgc cttgcaaagc gctcgggcac cctgagccag
     1261 attaccgctg gctgaaggac aacatgcccc tggaactttc agggaggttc cagaagaccg
     1321 tgacggggct gctcattgag aacattcgcc cctcggactc aggcagctat gtttgtgaag
     1381 tgtccaacag atacggaact gctaaggtga taggccgcct gtacgtgaaa cagccactga
     1441 aagccaccat cagtcccagg aaggttaaaa gcagcgtggg tagccaagtt tccttgtcct
     1501 gcagcgtgac aggaactgag gaccaggaac tctcctggta ccgcaatggt gaaatcctca
     1561 accctggaaa aaatgtgagg atcacaggga tcaaccacga aaaccttata atggatcaca
     1621 tggtcaaaag tgacgggggc gcataccagt gctttgtgcg caaggacaag ctgtccgctc
     1681 aagactatgt gcaggtggtc cttgaagatg gaactcccaa aattatttct gcctttagtg
     1741 aaaaggtggt gagtccagca gagccggttt cccttatgtg caacgtgaag ggaacacctt
     1801 tgcccacgat cacgtggacc ctggacgatg acccgattct caagggtggc agtcaccgca
     1861 tcagccagat gatcacgtcg gaggggaacg tggtcagcta cctgaacatc tccagctccc
     1921 aggtccggga cgggggagtc taccgctgca ctgccaacaa ctcggcggga gtcgtcctgt
     1981 accaggctcg aataaacgta agagggcctg caagcattcg accaatgaaa aacatcacag
     2041 caatagcagg acgggacaca tacattcact gtcgtgtgat tggctatccg tattactcca
     2101 ttaaatggta caagaactct aacctgcttc ctttcaacca ccgccaagtg gcatttgaga
     2161 acaatggaac tcttaaactt tcagatgtgc aaaaggaagt ggacgagggg gagtacacgt
     2221 gcaacgtgtt ggttcaacca caactctcca ccagccagag cgtccacgtg accgtgaaag
     2281 ttccgccttt catacaaccc tttgagtttc caagattctc cattgggcag cgggtcttca
     2341 tcccctgtgt tgtggtctca ggggacttac ccatcacgat cacctggcag aaggatggcc
     2401 ggccaatccc tgggagcctt ggggtgacca ttgacaatat tgacttcacg agctccttga
     2461 ggatttccaa tctctcgctc atgcacaatg ggaattacac ctgcatagcc cggaatgagg
     2521 ccgccgctgt ggagcaccaa agccagttga ttgtcagagt tcctcccaag tttgtggttc
     2581 agccacggga ccaggacggg atttatggca aagcagtcat cctcaattgt tctgctgagg
     2641 gttaccctgt acctaccatc gtgtggaaat tctctaaagg tgctggggtt ccccagttcc
     2701 agccaattgc cctaaatggc cgaatccaag ttctcagcaa tgggtcgttg ctgatcaagc
     2761 atgtcgtgga ggaagacagt ggctactacc tctgcaaggt cagcaacgat gtgggcgcag
     2821 acgtcagcaa gtccatgtac ctcacggtta aaattcctgc gatgataaca tcctatccaa
     2881 atactaccct ggccacgcag gggcagaaaa aggagatgag ctgcacggcg catggtgaga
     2941 agcccattat agtccgctgg gagaaggagg accgaatcat taaccctgag atggcccgtt
     3001 atcttgtgtc caccaaggag gtgggagaag aggtgatttc tactctgcag attttgccaa
     3061 ctgtgagaga agattctggt ttcttttcct gccatgctat taattcttat ggggaggacc
     3121 gtggaataat tcagctcaca gtgcaagagc ccccagaccc tcccgaaatt gagatcaaag
     3181 atgtcaaagc acgcacaatt acgctcaggt ggaccatggg gtttgatgga aacagtccca
     3241 tcacaggcta cgatattgaa tgcaaaaata aatcagactc ctgggattct gctcagagaa
     3301 ccaaagatgt ttcccctcag ctgaactcgg ccaccatcat tgatatccac ccttcctcca
     3361 cctacagcat ccgcatgtac gccaagaacc ggattggcaa gagcgagccc agcaacgagc
     3421 tcaccatcac ggcggacgag gcagctcctg atggtccacc tcaggaagtt cacctggagc
     3481 ctatatcatc tcagagcatc agggtcacat ggaaggctcc caagaaacat ttgcaaaatg
     3541 ggattatccg tggctaccaa ataggttacc gagagtacag cactgggggt aacttccaat
     3601 tcaacattat cagtgtcgac accagcgggg acagtgaggt ttacaccctg gacaacctga
     3661 ataagttcac tcagtacggc ctggtggtgc aggcctgtaa ccgggccggc acggggcctt
     3721 cttctcagga aatcatcacc accactctcg aggatgtgcc cagttacccc cccgaaaatg
     3781 tccaagccat agcaacatca ccagaaagca tatcaatatc ctggtccaca ctttccaagg
     3841 aagccttgaa tggaattctc caggggttca gagtcattta ctgggccaac ctcatggacg
     3901 gagagctggg tgagattaaa aacatcacca ccacacagcc ttcactggag ctggacgggc
     3961 tggaaaagta caccaactac agcatccagg tgctggcctt cacccgcgca ggagacgggg
     4021 tcaggagtga gcagatcttc acccggacca aagaggatgt tccaggtcct cccgcgggtg
     4081 tgaaggcagc ggcggcctca gcctccatgg tctttgtgtc ctggcttccc cctctcaagc
     4141 tgaacggcat catccgaaag tacactgtat tctgctccca cccctatccc acagtgatca
     4201 gcgagtttga ggcctctccc gactcgtttt cctacagaat tcccaacctg agtaggaatc
     4261 gtcagtacag cgtctgggtg gtggctgtta cttcagccgg aagaggcaac agcagtgaaa
     4321 tcatcacagt cgagccacta gcaaaagctc ctgcacgaat cctgaccttc agtgggacag
     4381 tgactactcc atggatgaaa gacattgtct tgccttgtaa ggctgttggg gacccttctc
     4441 ctgcagtcaa atggatgaaa gacagtaacg ggacacccag tctagtaacg attgatgggc
     4501 ggaggagcat ctttagcaac ggaagcttca ttattcgcac ggtgaaagca gaagactccg
     4561 gctattacag ctgcattgcc aataacaact ggggatctga tgaaattatt ttaaacttac
     4621 aagtacaagt tccaccagat cagcctcggc ttacagtctc caagaccacg tcttcctcca
     4681 tcaccctttc ttggctccct ggagacaacg ggggcagctc tatcagagga tacatactgc
     4741 agtactccga ggacaatagt gagcagtggg ggagttttcc aatcagcccc agcgaacgtt
     4801 cctatcgctt ggaaaatctc aaatgtggga cttggtataa gttcacactg acagcccaaa
     4861 atggagtggg cccagggcgc ataagtgaaa tcatagaagc aaagacctta ggaaaagagc
     4921 cccagttctc aaaggagcag gagctgtttg ccagcatcaa caccacacgc gtgaggctga
     4981 acctcattgg ctggaatgat ggcggctgcc ccatcacctc cttcacacta gagtacaggc
     5041 cctttgggac cacagtttgg accacagctc agaggacctc tctctccaag tcctacatcc
     5101 tgtatgacct gcaggaagcc acctggtatg agctgcagat gcgggtgtgc aacagtgcgg
     5161 gctgcgcgga gaagcaggcc aacttcgcta cgctgaacta cgatggcagt acaattcctc
     5221 cactcattaa gtcagttgtc caaaacgaag aagggctgac gaccaacgag gggctcaaga
     5281 tgctggtgac catctcctgt atcctggtgg gggtcttgct gctgtttgtg ctcctgctgg
     5341 ttgtgcggag gaggcggcgg gagcagaggc taaagaggct gcgagatgca aagagtttag
     5401 ctgaaatgct catgagtaag aatacccgga cttcagatac gttaagcaag caacagcaga
     5461 ccctgcgaat gcacatcgac atacccaggg ctcagctttt gattgaagag agagacacga
     5521 tggagaccat tgatgatcgc tccacggttc tgttgacgga tgctgacttt ggagaggcag
     5581 ctaagcagaa gtccctgacg gtcactcaca cggtccatta ccaatcggtg tctcaggcca
     5641 ctgggccctt agtggatgtt tcagacgctc ggccgggaac gaatcccacc accaggagga
     5701 atgccaaggc tgggcccaca gcgagaaacc gctatgccag ccagtggacc ctcaaccgac
     5761 cccaccccac catctcagca cacaccctca ccacagactg gaggctgcca acacccaggg
     5821 ctgcaggatc agtagacaaa gagagcgaca gttacagcgt cagcccctcg caagacacag
     5881 atcgagcaag aagcagcatg gtctccacag aaagtgcctc ctccacttac gaagaactgg
     5941 ccagggccta cgaacacgcc aagatggaag agcaactgag gcacgccaag ttcaccatca
     6001 cggagtgctt catatcagac acgtcatcgg agcagttgac ggcagggaca aatgagtaca
     6061 cggacagtct gacctccagc accccttccg aatcgggaat ctgcaggttc actgcatctc
     6121 cccccaaacc tcaggatgga ggaagagtaa tgaatatggc agttccaaag gcacatcggc
     6181 caggtgacct catacatttg cctccatacc ttagaatgga ctttttgtta aaccgaggtg
     6241 gtccaggcac cagcagggac ctgagcttag gacaagcatg cttggaacct cagaaaagcc
     6301 ggaccctgaa gcgccccacg gtcctggagc ccatcccgat ggaagccgcc tcctccgcct
     6361 cctccacgag agaaggacag tcgtggcagc cgggggccgt ggccacatta cctcagcggg
     6421 agggagcaga gctgggacag gcagctaaaa tgagcagctc ccaagaatca ctgctcgact
     6481 cccggggcca tttgaaagga aacaatcctt acgcaaaatc ttacaccctg gtataacaga
     6541 cagcatgact ggacagcggt tgtaaataca attcaaacaa ttcaatcaaa gctacctttt
     6601 ttttacggaa ttccaatatt tataattaaa gaaaattgcc aaaatatatt aaaaaaaaaa
     6661 aagagaaaat actgaaacca cagacagtgc aaagactctc ctgctttttt tctgtctgag
     6721 tgtgagctcc atccgcctgg gcatctgatt ttttgggaaa gaagtccagt tttatgatct
     6781 tcacaggcta ttgcattttt actgattttc tacaaaagtg catggggggg attaattaaa
     6841 ccttctgact ggaagttcta tcacacaaga ggcaagaaag aaaacagggt gggtgggaaa
     6901 tcagcctatt tgtgaattta aaaggaacac cgatttgcgg gcaggggaaa acttcagtca
     6961 cgtttgcaca cctttctttc ccattagaaa ccgggttctc aatttggtct tcttttgttg
     7021 ttaattagga tgagtgccgt cagtgaaggg gtggggggaa tcaatttggt tttctctctt
     7081 gtttcttttt taatttaatg agacactctt tgcattttgt ctaagcgaaa taaaaaagaa
     7141 aaggttgtct gcctttattt ccatgtctgc ttctctcgtc ttctgccagt ggcctggtcc
     7201 tccttcccat tgacacttct gtttggaaac accaggcctg aacccagagc ccaattcaat
     7261 aaaccagagt cgatactaac accctgaact cctcaggaat ctccaggaag cacaaagaag
     7321 gtgcagctcc tgctctcaag ctggagagga caaaatgcag gctggtctcc accgcaacca
     7381 gaggccagcc caagtattcg aagcccgtcg caaagctctg ccccttgcct gcccatggcc
     7441 agagttcaac tgactgtggc ctatgggcca aatccagcct cctgctgttt tttaaataaa
     7501 gttttattga aacaaggcca catgcactat ttgctattgt ctatggctgc tttcatggca
     7561 caatggtaga gctgaatagt tgcagcagag atggtatagc ccatgaagcc taaaatattt
     7621 attacctggc ccattacagg aaacatttgc cagacccgtg ctctggagtc tgaaatgtag
     7681 ccccagcttg cgtgacaagg ggcaagatcg tgcatcttac cctccatgac ttttcctatc
     7741 tccactatgc ctggtatgca aatttgtgat agaccatctt aataaacatt gcctgaatcc
     7801 tctgtaacca gaggaaagtg atgttcctct aaaatctaag tttttacttt cctttccctc
     7861 actttatctc ccatgtggct catcctaaga gagaatttac tcataaatag tcggaactgc
     7921 attctttatc ccacaaccct cacagggttt gctttatgaa agaggatgta atcctcccct
     7981 ggctgcaaca ctccattcca ggctcacttc cataaaatgg aaagataaac aggtctttgt
     8041 gtcagagatt tggcattaac cagaaaacat gatcaaatat ccacagctgc tcagtctccc
     8101 aggctgaaga ttctctacag gcttccatta aggccaccaa catagactgg tgccagttat
     8161 taccatcttg ggaacatatt cagaaatgtc tttccttccc tccctccctc cttctttccc
     8221 atcctttctt ccttctattt attttccagg caggcccatt tgtctgctta gattcagtga
     8281 cacagaatgg gtccaggacc atgtcaagac cacctgctga tgctagtgtt agatgactcc
     8341 aggaatgtga ggtgtgcatc catggtttca gaatgtaatc cgtgttgacg tgtactgttt
     8401 gcatactctc agactgtttt ttttttatgc aaaagaaatg ttactttctg ggggaaaaaa
     8461 ttaaaagtct atctttcatc aacctaaaac ttctatatcc cccaaacata gaagtttacc
     8521 tttttaacat ttttctctca acctgtacaa aaataaaaaa attctacatt a
//
