LOCUS       NM_024422              12331 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens desmocollin 2 (DSC2), transcript variant Dsc2a, mRNA.
ACCESSION   NM_024422
VERSION     NM_024422.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Han,J.Y., Che,N., Mo,J., Zhang,D.F., Liang,X.H., Dong,X.Y.,
            Zhao,X.L. and Sun,B.C.
  TITLE     Desmoglein 2 and desmocollin 2 depletions promote malignancy
            through distinct mechanisms in triple-negative and luminal breast
            cancer
  JOURNAL   BMC Cancer 24 (1), 532 (2024)
   PUBMED   38671389
  REMARK    GeneRIF: Desmoglein 2 and desmocollin 2 depletions promote
            malignancy through distinct mechanisms in triple-negative and
            luminal breast cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  AUTHORS   Sun,C., Wen,K., Zhang,B., Dong,Y., Chen,C., Neo,S.Y., Leng,B.,
            Gao,T.T. and Wu,J.
  TITLE     DSC2 suppresses the growth of gastric cancer through the inhibition
            of nuclear translocation of gamma-catenin and PTEN/PI3K/AKT
            signaling pathway
  JOURNAL   Aging (Albany NY) 15 (13), 6380-6399 (2023)
   PUBMED   37421607
  REMARK    GeneRIF: DSC2 suppresses the growth of gastric cancer through the
            inhibition of nuclear translocation of gamma-catenin and
            PTEN/PI3K/AKT signaling pathway.
REFERENCE   3  (bases 1 to 12331)
  AUTHORS   Pariyar,M., Thorne,R.F., Scott,R.J. and Avery-Kiejda,K.A.
  TITLE     Verification and Validation of a Four-Gene Panel as a Prognostic
            Indicator in Triple Negative Breast Cancer
  JOURNAL   Front Oncol 12, 821334 (2022)
   PUBMED   35387118
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12331)
  AUTHORS   Chen,S.Y., Lih,T.M., Li,Q.K. and Zhang,H.
  TITLE     Comparing Urinary Glycoproteins among Three Urogenital Cancers and
            Identifying Prostate Cancer-Specific Glycoproteins
  JOURNAL   ACS Omega 7 (11), 9172-9180 (2022)
   PUBMED   35350332
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12331)
  AUTHORS   Syrris,P., Ward,D., Evans,A., Asimaki,A., Gandjbakhch,E.,
            Sen-Chowdhry,S. and McKenna,W.J.
  TITLE     Arrhythmogenic right ventricular dysplasia/cardiomyopathy
            associated with mutations in the desmosomal gene desmocollin-2
  JOURNAL   Am J Hum Genet 79 (5), 978-984 (2006)
   PUBMED   17033975
REFERENCE   6  (bases 1 to 12331)
  AUTHORS   Nuber,U.A., Schafer,S., Schmidt,A., Koch,P.J. and Franke,W.W.
  TITLE     The widespread human desmocollin Dsc2 and tissue-specific patterns
            of synthesis of various desmocollin subtypes
  JOURNAL   Eur J Cell Biol 66 (1), 69-74 (1995)
   PUBMED   7750520
REFERENCE   7  (bases 1 to 12331)
  AUTHORS   McNally,E., MacLeod,H. and Dellefave-Castillo,L.
  TITLE     Arrhythmogenic Right Ventricular Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301310
REFERENCE   8  (bases 1 to 12331)
  AUTHORS   Arnemann,J., Spurr,N.K., Wheeler,G.N., Parker,A.E. and Buxton,R.S.
  TITLE     Chromosomal assignment of the human genes coding for the major
            proteins of the desmosome junction, desmoglein DGI (DSG),
            desmocollins DGII/III (DSC), desmoplakins DPI/II (DSP), and
            plakoglobin DPIII (JUP)
  JOURNAL   Genomics 10 (3), 640-645 (1991)
   PUBMED   1889810
REFERENCE   9  (bases 1 to 12331)
  AUTHORS   Parker,A.E., Wheeler,G.N., Arnemann,J., Pidsley,S.C., Ataliotis,P.,
            Thomas,C.L., Rees,D.A., Magee,A.I. and Buxton,R.S.
  TITLE     Desmosomal glycoproteins II and III. Cadherin-like junctional
            molecules generated by alternative splicing
  JOURNAL   J Biol Chem 266 (16), 10438-10445 (1991)
   PUBMED   2037591
REFERENCE   10 (bases 1 to 12331)
  AUTHORS   Garrod,D.R. and Fleming,S.
  TITLE     Early expression of desmosomal components during kidney tubule
            morphogenesis in human and murine embryos
  JOURNAL   Development 108 (2), 313-321 (1990)
   PUBMED   2112455
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC382631.1, BC063291.1,
            X56807.1, BE350090.1 and AC012417.13.
            
            On May 20, 2019 this sequence version replaced NM_024422.5.
            
            Summary: This gene encodes a member of the desmocollin protein
            subfamily. Desmocollins, along with desmogleins, are cadherin-like
            transmembrane glycoproteins that are major components of the
            desmosome. Desmosomes are cell-cell junctions that help resist
            shearing forces and are found in high concentrations in cells
            subject to mechanical stress. This gene is found in a cluster with
            other desmocollin family members on chromosome 18. Mutations in
            this gene are associated with arrhythmogenic right ventricular
            dysplasia-11, and reduced protein expression has been described in
            several types of cancer. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Feb 2015].
            
            Transcript Variant: This variant (Dsc2a, also known as DGII)
            encodes the longer isoform (Dsc2a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063291.1, SRR14038192.2462055.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280904.11/ ENSP00000280904.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-572               DC382631.1         12-583
            573-3157            BC063291.1         566-3150
            3158-3566           X56807.1           2794-3202
            3567-5130           BC063291.1         3560-5123
            5131-5197           BE350090.1         7-73                c
            5198-12331          AC012417.13        123479-130612
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..12331
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /note="desmocollin 2"
                     /db_xref="GeneID:1824"
                     /db_xref="HGNC:HGNC:3036"
                     /db_xref="MIM:125645"
     exon            1..519
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             451..3156
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /note="isoform Dsc2a preproprotein is encoded by
                     transcript variant Dsc2a; desmosomal glycoprotein II/III;
                     cadherin family member 2"
                     /codon_start=1
                     /product="desmocollin-2 isoform Dsc2a preproprotein"
                     /protein_id="NP_077740.1"
                     /db_xref="CCDS:CCDS11892.1"
                     /db_xref="GeneID:1824"
                     /db_xref="HGNC:HGNC:3036"
                     /db_xref="MIM:125645"
                     /translation="MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLD
                     AEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTE
                     NQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQ
                     SDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTP
                     DGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTM
                     HTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFG
                     LQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRAN
                     YTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPR
                     SAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRY
                     KKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGII
                     LQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWR
                     LKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVD
                     PRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVS
                     NTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCR
                     GAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKVYLCNQDENHKHAQDYVLT
                     YNYEGRGSVAGSVGCCSERQEEDGLEFLDNLEPKFRTLAEACMKR"
     exon            520..604
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            605..804
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            805..924
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1080
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1225
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1392
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1527
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1713
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1970
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2113
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2338
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2575
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2700
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2958
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..12331
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5177..5182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /note="hexamer: AATAAA"
     polyA_site      5195
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /note="major polyA site"
     regulatory      12311..12316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
                     /note="hexamer: ATTAAA"
     polyA_site      12331
                     /gene="DSC2"
                     /gene_synonym="ARVD11; CDHF2; DG2; DGII/III; DSC3"
ORIGIN      
        1 agtcagggct tctgctgggc agggccggct gttaatctcg cctggcggag cggacaccgg
       61 ggcgtggtgg gggagatggg ccttataggt ggcgtccgct ggtgagaaac accttgcgca
      121 ggtaaaaggg tggcggcgag agggagttcc cacccgtggc tttcttagag aaatgaagtc
      181 ttaagtctta aatagacaac aaggaggggc cgatcggtgt cttttggacg cgtctggagc
      241 ccctccctcc gccaaaggaa aagccccttg gatgagaggc aggcgcttca gagaagctaa
      301 gaaaagcacc tctccgcgcg ccccacctcc tccgcctcgc gctcctcctg agcagcgggc
      361 ccagactgcg ctccggccgc ggccctcgcc ccgcggagcc ctcctacccc ggcccgacgc
      421 tcggcccgcg acctgccccg agccctctcc atggaggcag cccgcccctc cggctcctgg
      481 aacggagccc tctgccggct gctcctgctg accctcgcga tcttaatatt tgccagtgat
      541 gcctgcaaaa atgtgacatt acatgttccc tccaaactag atgccgagaa acttgttggt
      601 agagttaacc tgaaagagtg ctttacagct gcaaatctaa ttcattcaag tgatcctgac
      661 ttccaaattt tggaggatgg ttcagtctat acaacaaata ctattctatt gtcctcggag
      721 aagagaagtt ttaccatatt actttccaac actgagaacc aagaaaagaa gaaaatattt
      781 gtctttttgg agcatcaaac aaaggtccta aagaaaagac atactaaaga aaaagttcta
      841 aggcgcgcca agagaagatg ggctccaatt ccttgttcga tgctagaaaa ctccttgggt
      901 ccttttccac ttttccttca acaggttcaa tctgacacgg cccaaaacta taccatatac
      961 tattccataa gaggtcctgg agttgaccaa gaacctcgga atttatttta tgtggagaga
     1021 gacactggaa acttgtattg tactcgtcct gtagatcgtg agcagtatga atcttttgag
     1081 ataattgcct ttgcaacaac tccagatggg tatactccag aacttccact gcccctaata
     1141 atcaaaatag aggatgaaaa tgataactac ccaattttta cagaagaaac ttatactttt
     1201 acaatttttg aaaattgcag agtgggcact actgtgggac aagtgtgtgc tactgacaaa
     1261 gatgagcctg acacgatgca cacacgcctg aagtactcca tcattgggca ggtgccacca
     1321 tcacccaccc tattttctat gcatccaact acaggcgtga tcaccacaac atcatctcag
     1381 ctagacagag agttaattga caagtaccag ttgaaaataa aagtacaaga catggatggt
     1441 cagtattttg gtctacagac aacttcaact tgtatcatta acattgatga tgtaaatgac
     1501 cacttgccaa catttactcg tacttcttat gtgacatcag tggaagaaaa tacagttgat
     1561 gtggaaatct tacgagttac tgttgaggat aaggacttag tgaatactgc taactggaga
     1621 gctaattata ccattttaaa gggcaatgaa aatggcaatt ttaaaattgt aacagatgcc
     1681 aaaaccaatg aaggagttct ttgtgtagtt aagcctttga attatgaaga aaagcaacag
     1741 atgatcttgc aaattggtgt agttaatgaa gctccatttt ccagagaggc tagtccaaga
     1801 tcagccatga gcacagcaac agttactgtt aatgtagaag atcaggatga gggccctgag
     1861 tgtaaccctc caatacagac tgttcgcatg aaagaaaatg cagaagtggg aacaacaagc
     1921 aatggatata aagcatatga cccagaaaca agaagtagca gtggcataag gtataagaaa
     1981 ttaactgatc caacagggtg ggtcaccatt gatgaaaata caggatcaat caaagttttc
     2041 agaagcctgg atagagaggc agagaccatc aaaaatggca tatataatat tacagtcctt
     2101 gcatcagacc aaggagggag aacatgtacg gggacactgg gcattatact tcaagacgtg
     2161 aatgataaca gcccattcat acctaaaaag acagtgatca tctgcaaacc caccatgtca
     2221 tctgcggaga ttgttgcggt tgatcctgat gagcctatcc atggcccacc ctttgacttt
     2281 agtctggaga gttctacttc agaagtacag agaatgtgga gactgaaagc aattaatgat
     2341 acagcagcac gtctttccta tcagaatgat cctccatttg gctcatatgt agtacctata
     2401 acagtgagag atagacttgg catgtctagt gtcacttcat tggatgttac actgtgtgac
     2461 tgcattaccg aaaatgactg cacacatcgt gtagatccaa ggattggcgg tggaggagta
     2521 caacttggaa agtgggccat ccttgcaata ttgttgggca tagcattgct cttttgcatc
     2581 ctgtttacgc tggtctgtgg ggcttctggg acgtctaaac aaccaaaagt aattcctgat
     2641 gatttagccc agcagaacct aattgtatca aacacagaag ctcctggaga tgacaaagtg
     2701 tattctgcga atggcttcac aacccaaact gtgggcgctt ctgctcaggg agtttgtggc
     2761 accgtgggat caggaatcaa aaacggaggt caggagacca tcgaaatggt gaaaggagga
     2821 caccagacct cggaatcctg ccggggggct ggccaccatc acaccctgga ctcctgcagg
     2881 ggaggacaca cggaggtgga caactgcaga tacacttact cggagtggca cagttttact
     2941 cagccccgtc ttggtgaaaa agtgtatctg tgtaatcaag atgaaaatca caagcatgcc
     3001 caagactatg tcctgacata taactatgaa ggaagaggat cggtggctgg gtctgtaggt
     3061 tgttgcagtg aacgacaaga agaagatggg cttgaatttt tggataattt ggagcccaaa
     3121 tttaggacac tagcagaagc atgcatgaag agatgagtgt gttctaataa gtctctgaaa
     3181 gccagtggct ttatgacttt taaaaaaaat tacaaaccaa gaatttttta aagcagaaga
     3241 tgctatttgt gggggttttt ctctcattat ttggatggaa tctctttggt caaatgcaca
     3301 tttacagaga gacactataa acaagtacac aaatttttca atttttacat atttttaaat
     3361 tacttatctt ctatccaagg aggtctacag agaaattaaa gtctgcctta tttgttacat
     3421 ttgggtataa tgacaacagc caatttatag tgcaataaaa tgtaattaat tcaagtcctt
     3481 attatagact atttgaagca caacctaatg gaaaattgta gagaccttgc tttaacatta
     3541 tctccagtta attaagtgtt catgtggtgc ttggaaactg ttgttttcct gaacatctaa
     3601 agtgtgtaga ctgcattctt gctattattt tattcttgta atgtgacctt ttcactgtgc
     3661 aaagggagat ttctagccag gcattgacta ttacaatttc attttggtgg agtttagttt
     3721 taggttttat tgtatataaa atcctgcact gaatctgtgt ctcctctgtt acctactttt
     3781 gccagtgaaa tttaagtttt aaaatacttt cagaatgtat ttttactact gcaagttttt
     3841 ggtctttaaa atgtcaagta gcatctctct ctttctctct gtctctttct gtttctctct
     3901 ccagtttttt tttttttttt aatttccata tgggctaaag aatccaaata ttttaaaaat
     3961 ctgtctctct tttcttctct cataaagtga attattcctt ttttttgttt tatgtaagtg
     4021 tatatattct tagtttttct tgaaatcatt gtaatgttaa ctttgttgtt tcaaatatct
     4081 tggtgattgc ttcattatct cttcaacaaa aaaaaccttt aattttgcca ttgaaactgt
     4141 agaactatgc catgctttta ttagaagcag tgctctgtgt taacaacaag aatggtgtaa
     4201 ttagaattgg gatgtggata tttactgtat gacaacacat ttacagttct gtaatgcaag
     4261 gatgcagttt aaaaatgtga agtagtgatg gtttttgaaa taagctttaa aatataggga
     4321 tcttgaaggc tccctggggt aactatttta taacttagat aaaatggcta gtcatatctg
     4381 tgtgtttgta aagttatttt tttaatattt taagattaca attttaacaa atgtagaaat
     4441 gagccaaact atttaaattt taaaacagta aaacaaaatg aaacttaata gctcacaaaa
     4501 ttccagtcca tgtttcatga cttattttag tcaatgaatt ttctatttat actaaacata
     4561 tggacatttt aaatgtgttt ctaatatttt tgattatcta taatgtgcct gtcttcaatt
     4621 cacaagattg ggttataaca attatttgcc agattaacac tagggaatta tttgataacc
     4681 agcttatctt atcagtagtt ttattgctga tcaggcaaaa atagttttcc aaagttattt
     4741 ttaataaagt atatacaaaa ttcttatata ttactagtca tgataaagta aattaagcag
     4801 tttttaaaac ttagtgtgag tttgttcatc acaggtctga tatgagttta agggatttcg
     4861 cactccctga atcagagaag taagacccct tccttagatt cctgttatac attttttaaa
     4921 atgtagagtt tgttttggag acattttcag tgcattgtta ttgccatatt tatataatat
     4981 gactattcta aaggctgtga ggccatgggg tattggttaa gttgcttgct tttgctttgt
     5041 ccattttcat cattttaaaa tgggggataa taacagaact tgtttcctag ggccattgta
     5101 agtcacttga ataaaaaata gttttgaagc atgagagtca tacagagcgg tccacctaaa
     5161 aggcactcct gataataata aatgatttta aacaagtcac atgtacaatt catttcatgt
     5221 taacaaaagg tcaattaaga caataagata ctgaaaagat ttttcttttc actgtagaac
     5281 atgtctcttc ataatgatca cctgatgact gggtcttgta tgttcagttt ttcagaatac
     5341 gtagtgcaaa gattgctgag gcattcgtat gggattgcct ggtttgaaac actgtgaaac
     5401 tgtaaggttc aaattggatg aaaagcagaa catctgcttc ttaaaatggg cactaattct
     5461 aacaccctat gaaaattatg tgtattttag attctccctg tcaatccctt ccttggcttc
     5521 ttccctagct gtccagctag acattagcag tcacttgcct gggaggtcat ggctgtttgg
     5581 cagtcgtcat agtaacccca agtgctaaga cttccaagta agagtgtctt gatgcaaatt
     5641 ccggctctat ccttactagc tgtgggattt gagaagtcac ttaagctttc tgtgcttagt
     5701 ttccttactt ataggacctg tactgaggag ggtagaggag tctacttgac agggctttgt
     5761 cagagttaat tcatgaaaag agttggcata gagttagcac tcaatcatgt accttagcca
     5821 ctgctattgt tctatgagca gagaacgcct gagttgggaa caatttagct tcagtctcca
     5881 ctctgcttta ctgctgacat cttgccatga tgctcttatt cacctttccc agaagcccga
     5941 ccagtatttc accagtgatg gcctttggga atttcatggc tcatagtagc ttccaggtct
     6001 ctcatttgtg gtattcagca agttacatct tattcatccc tagccagaag tcagtgtgat
     6061 aaatcttgat gcttaactga aaaggcaggt aaaattctcg aggaggcaaa atcataaaga
     6121 gtttcccgaa gaaatggctg gtgcttcttc agcaaatgaa aagcagcaag atcttctgtt
     6181 ccatgcagtg tgtgtctggg ggctcctggt aatcattact ttttctttat tcccatctta
     6241 agactgtttt tatcttggat cccaattctg ttttgccaaa tctgactttg actacgggct
     6301 tcccttttgt ctccatctcc attcctggac tccagattga agtcttgaca actgactttg
     6361 ctctgatcag cccctttagt atcatctcca aaataaaatg ctttcccttc tttgtgccta
     6421 gaatcatctt gtttttcacc tccttaaata ttcaaagccc taagaagtta cctgatatcc
     6481 gtataaacct acagctctta tatcagctcc cctgcgagcc ccctgttaac ctagcacttc
     6541 aaactcatgc aaaaagacaa acacatacta ccacatcaaa tcaggttctc ctgagttatt
     6601 tacacaatgg tgcccttctt ccacttactt aggcttgaaa cagagaatcc ttgattcctc
     6661 tctcttcttt ccatagcatc ctctattttt tgccaaaaac tgccaaactt ttgagaattg
     6721 tagacacaat ttcattcatt aaatactttg tttgtactgt agtcatttga tgccttccca
     6781 gtgcagtcat atgtagcctt ttaaaacaga gactagaagg actgtgcttc tatgagaaag
     6841 tgcaagatga atcagccact ctccccaccc acacacttga atgcaagtca agacacggtg
     6901 gatgtaagga gtgggctaat gcctgtaaaa cacatcacac aatgcctggc acatagtaag
     6961 tgcataatgc atgtttttaa aaagcttcag atgatgtgaa agaacttcag gacactagtg
     7021 agtgaacttc tgcttctgct actcccaatc caagcattca tgaggttgag gtgtacccta
     7081 tgtcagctgt tcccactctt cacttcattc ctgatcaata ttcccccccg ccagtacccc
     7141 agattaaatc ttcagcatca ctcagctcca atggtgttta tgcttccaat ttcttccctc
     7201 ttctcttttt tcaagggcca tcaaagagca gatatgatca tgccattctg cctaagagct
     7261 ttctataggg ttttagagga taaattctaa acacaaaatc acttttaaat acagatgctt
     7321 cttgacttat gatggggtta catcctgata aacccatggt aaattgaaaa tattacaaat
     7381 caaaacagca ttttccattt accatatgtt caacttaaaa taagcttatt gggatgtagt
     7441 ctcataagtg taggagaatg ctgaatgcat atttctttga caccatcata aagttgaaat
     7501 atcagatcga gccatcttaa gttgaggacc ttctgtacta atttccccaa tctccttgta
     7561 tccttcattt ttcaatttgt gccttgctca tgattagttc tctttttaca atgtctttac
     7621 tcattgtctc tttctttgta aaaatattac tttttgagga tctagtaaaa atactaccgc
     7681 ctagacattt gtgtgtctag agcacatggc tgtaaagttt tcatctacag gtttgtgtgt
     7741 gtgtttcttt tctttcatgt aggacactaa ggaccaagaa aggtgttcac aattgcatgc
     7801 ctatacaact ctggccatta attttttttt tttttttttt gagacggaat ctcactctgt
     7861 cacctaggct ggagtgcagt ggcatgatca tggctcactg caacctctgc ctcccaggtt
     7921 caagcaattc ttctgcctca gcctcctgag tagctgggat tacaggcacc taccaccacg
     7981 cctggttaat ttttgtattt ttagcagaga aagggtttca ccatgttggc caggctggtc
     8041 ttgaactcct gacctcaagt gatctgtcag cacttggcct cccaaagtgc tgggattaca
     8101 ggcataagcc actgtgcctg gccaaatttt gattcatcaa attatattgc ccaggtcata
     8161 aaggaaaaaa gagaaataat aaaagttagg ttttttattc tcagcacata ctaattttct
     8221 gtggcataaa ttttacatga tagacatggt gtatgttaga ttctgcactg aattgttttt
     8281 cctcagaaga cattgaatga ctagaacaat tgtttgcatt cacagcttaa tacaggaaag
     8341 tgaagtgttg agattttgga atcccttctc ccttgggata gtgagagggg cagacagaag
     8401 gggtagtgtc tgaatattgt ggttcaggcc agctcaaatc ctaccaccaa taattgcacg
     8461 aacctacttg tagccaccaa taattgcccc atattgaaat gtaaccccca agggttctcc
     8521 tctgtcttct tgaccactgt atgcctaaca tgtagagcag tagacactga atatatttgg
     8581 tgtgtgatga ataaatggat gaatgaataa atgcacatgt gtgttaggta cctggctaag
     8641 atcagactgc atattgccta aagaattctt tttctcaact tgaaaatttt tagccaatgt
     8701 gaaaattatg tttgcgactg tcacttcatc attgccaggt tcaaacactg cagtgaattt
     8761 ttttttttta accaaaaggc cactctctaa ccacctgagg aaaagcaact aaaaaccact
     8821 tatgtttaga aattcaagaa atgattctta tgagtttgcc aaaattccca ttagttattt
     8881 ataaaatgtt gtaagtcagg ccttgcttca aaaaatatct tgagaaacca caaagcaaag
     8941 aaagtggcat acatctacga gctttttcca tgttaggctg ggagaacatt ctgtaacagg
     9001 aaaccaccgt gctcatgtgt gccctgagag aagaaaatct tcacacaaga agtagagaat
     9061 agggaaagaa atgcatagca atgagcctca gaggcaatgc ctatattgtg ctgtgaactg
     9121 ataatgtcac ttggaagata acacgtaaga ggtagttccc agccctgctc ttctcacgta
     9181 ggacatgtag gacagaccta tgtgttttgc tttctgggga gaggggatca gttgtatgac
     9241 cccctttatt atttcctctt caccagttct ggttttctag gtttgaggac tgaaatcttg
     9301 agaagtagga tctgatcttt cttccaagaa gctcttctct ccccacctgt ccttgtgttc
     9361 tttatcccat caatagctaa gaagcagaac ctattacttt tgcttaaatg ttttaaatta
     9421 agcaaataga tacattatga ggctgcattt taaactttag atatttcaaa taagacatgc
     9481 aatatagagt cttaaggtaa aacaaaagag cataatgcta aaaaacaggc aacaaaaaga
     9541 agctagagct ttgaaatagc tagtcaggta aacagagcac tttaatttaa aagaacacac
     9601 cctaaatctt gttttataga ggaattttac tgtgtcatgg atttggagca tattcttctc
     9661 aagccatttg gtatatccta aattctaggt atacgtgaga ttaaagaaga gatactcact
     9721 gatgtgcctc cgtatggagt gatagttggt gcatgggaaa tggcaagggc tgttttgttt
     9781 cattctctct tccctttgtg actgtacctt tgtatcccta cagaaaatct gggcatagct
     9841 gagaggttag aattgtggag tttctgctag ccacatcttt tctgtttact tattttgtta
     9901 ggattcaagg gagaggacag agcagaacac tcactgcttt tcagttaaca aatatgaagg
     9961 caaacttgct gatatattcg cttgtataat ttttagagtg tgtttcactg accatcagca
    10021 tcagaatcac cctggtgttg ctgaaaatag agatccctca tgaatcagtc acaggagtaa
    10081 caggctagta ggggaaaagt atagaagcct tcacttttca atacattttc taggtcattt
    10141 tttcttactc taaagtttga caatcgttag ggttttagta actgggttta ttgattattt
    10201 ggtggtttat gaaacttaag tatttaaaat ttgccttttc atataaaaca tattaagcaa
    10261 aatgctagaa atgtaccaaa gagttgaaac acattgatga gtaaaatttc acaatgtctg
    10321 caaattaagc atttcagaat gtacattgtt ccctgtggtt tctactttct acatgcatgt
    10381 gaacgagaga aatagtctta aggtaggaat gcagaacaaa gccacacaga aaacatttaa
    10441 taaaaattaa caagttttgc cacataaatg ctgctagtca agaagatgta tgcaaagtaa
    10501 gttgcatata tttttaaaaa tcagaattgc caaatctcca gagataaata gaactagaaa
    10561 ggtcaacagc aggtggaaca agtgactgtc cagaaggcag caactgtttg gcatcactgt
    10621 gctgttgtgt ggggacctgt gtcaggtggg gtaacaggta atgctcactg actgtggcgg
    10681 ccaggagctt cagaggggcc tgtgaatcag ggatgggaga tgagggtaga gacgagactt
    10741 ttcagcttaa ggagtgttcc cctgggaaca gggccaattg gaaaactccc aggcaccaag
    10801 gacatgagtt aaccaatagg gaatgagagg ctcagaaagc cagaggtgaa aggagcagag
    10861 ggtgtcaata tacacaagat gtgatgcaga attaccaatg aaatcaagta aaagggattt
    10921 atccaaaaga agtaagagaa tagtgtaaag attctactca catgcaagga tgtttgtctc
    10981 agtggcatta aatatatgaa taactgaaaa taaactaaat gttccatgac agggaagtaa
    11041 ttaaatgatg tatgatccat tactgtgata caatgtacca taattgagga caatggaaac
    11101 aatgatcaca cacagacttc gcagtcagaa agacgtggat ccaaatctca gctttgttct
    11161 tgctgtgtta ccttgggaaa tcacagaaac ctcaaatttc tagcatatca aataaggaca
    11221 aattaggaat aaattatata attcacataa agatcctagc ttactgcttg gctcatcagt
    11281 atatcaataa ccagcagcta tcactattgt gatatgatga tggaatatca tgcaatcatt
    11341 ttattttgaa aactttaatt aaatgggaaa agacataaac tatagtagta ttttaaaact
    11401 aggataggtt tccaaattat tataatctca catttaaata aatatgagac tatctgtaat
    11461 acactaagat attgagtgat aaatcctcgg taaatgaaat atttattcat tgtgtgtttc
    11521 catgttttca ttttaccaca agcatttata atttataaca ttgagggaaa agttcatgta
    11581 aatgaaatac agagcaagaa aaactcacag gctttagaat ttggggagct tacaggcctg
    11641 tgaagaaaag ccttttcatc ttagctttaa taaataaagt tttcactgaa catattatat
    11701 ttacagtaaa tgattaaatt gaaagcaaaa agaaaatact gtatcatctc acattatcat
    11761 aactgagtgc attcatgtgc ttttggtctt aaaattttac tttaacgcac ttaggatttg
    11821 tcgtcagtaa tttgttatgc actatctaga ctaagaatag gtattaattt actagaattg
    11881 tcaaatgaaa tatttcaagc aaagtttgca taatgaatgc aagtttaaac caattaaatg
    11941 tgttcacttt ttagcaagct aagctgctcc ttatttctcc tgtagtagac acaatcacag
    12001 ttaatctttg cacaatgcat cgcattctcc atgcaaccta acagagcggt ctgggataga
    12061 gcctgaactt tgggtccctt ggggcccaac aggcaaggtt cagatctggg ctctaccaca
    12121 aaccatctgt gtaacctgaa cctccctagt cttcagtttc ctcatctgct ttaaaaaatt
    12181 gctagaacat agttctatgt aaatgttcgg tgttaaaatt tatttggtat ctactgtatg
    12241 taagatatgg cctctgctct caaaaatatg tagtcccaga agtgatataa tatgttcacg
    12301 gagacgtact attaaaaagt gccacaaaaa a
//
