LOCUS       NP_001369437            1374 aa            linear   PRI 20-FEB-2025
DEFINITION  ribonuclease 3 isoform 1 [Homo sapiens].
ACCESSION   NP_001369437 XP_005248348
VERSION     NP_001369437.1
DBSOURCE    REFSEQ: accession NM_001382508.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1374)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1374)
  AUTHORS   Noguchi,S., Ohkura,S., Negishi,Y., Tozawa,S., Takizawa,T.,
            Morita,R., Takahashi,H., Ohkuchi,A. and Takizawa,T.
  TITLE     Cytoplasmic and nuclear DROSHA in human villous trophoblasts
  JOURNAL   J Reprod Immunol 162, 104189 (2024)
   PUBMED   38241848
  REMARK    GeneRIF: Cytoplasmic and nuclear DROSHA in human villous
            trophoblasts.
REFERENCE   3  (residues 1 to 1374)
  AUTHORS   Kondkar,A.A., Azad,T.A., Sultan,T., Osman,E.A., Almobarak,F.A.,
            Lobo,G.P. and Al-Obeidan,S.A.
  TITLE     The 3' UTR polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA
            genes are not associated with primary open-angle and angle-closure
            glaucoma: As case-control study
  JOURNAL   PLoS One 18 (4), e0284852 (2023)
   PUBMED   37099569
  REMARK    GeneRIF: The 3' UTR polymorphisms rs3742330 in DICER1 and rs10719
            in DROSHA genes are not associated with primary open-angle and
            angle-closure glaucoma: As case-control study.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 1374)
  AUTHORS   Cressoni,A.C.L., Penariol,L.B.C., Padovan,C.C., Orellana,M.D.,
            Rosa-E-Silva,J.C., Poli-Neto,O.B., Ferriani,R.A., de Paz,C.C.P. and
            Meola,J.
  TITLE     Downregulation of DROSHA: Could It Affect miRNA Biogenesis in
            Endometriotic Menstrual Blood Mesenchymal Stem Cells?
  JOURNAL   Int J Mol Sci 24 (6), 5963 (2023)
   PUBMED   36983035
  REMARK    GeneRIF: Downregulation of DROSHA: Could It Affect miRNA Biogenesis
            in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1374)
  AUTHORS   Kamalabad,S.T., Zamanzadeh,Z., Rezaei,H., Tabatabaeian,M. and
            Abkar,M.
  TITLE     Association of DROSHA rs6877842, rs642321 and rs10719 polymorphisms
            with increased susceptibility to breast cancer: A case-control
            study with genotype and haplotype analysis
  JOURNAL   Breast Dis 42 (1), 45-58 (2023)
   PUBMED   36911928
  REMARK    GeneRIF: Association of DROSHA rs6877842, rs642321 and rs10719
            polymorphisms with increased susceptibility to breast cancer: A
            case-control study with genotype and haplotype analysis.
REFERENCE   6  (residues 1 to 1374)
  AUTHORS   Lee,Y., Ahn,C., Han,J., Choi,H., Kim,J., Yim,J., Lee,J.,
            Provost,P., Radmark,O., Kim,S. and Kim,V.N.
  TITLE     The nuclear RNase III Drosha initiates microRNA processing
  JOURNAL   Nature 425 (6956), 415-419 (2003)
   PUBMED   14508493
  REMARK    GeneRIF: identification of another RNase III, human Drosha, as the
            core nuclease that executes the initiation step of miRNA processing
            in the nucleus
REFERENCE   7  (residues 1 to 1374)
  AUTHORS   Fortin,K.R., Nicholson,R.H. and Nicholson,A.W.
  TITLE     Mouse ribonuclease III. cDNA structure, expression analysis, and
            chromosomal location
  JOURNAL   BMC Genomics 3 (1), 26 (2002)
   PUBMED   12191433
  REMARK    Publication Status: Online-Only
REFERENCE   8  (residues 1 to 1374)
  AUTHORS   Wu,H., Xu,H., Miraglia,L.J. and Crooke,S.T.
  TITLE     Human RNase III is a 160-kDa protein involved in preribosomal RNA
            processing
  JOURNAL   J Biol Chem 275 (47), 36957-36965 (2000)
   PUBMED   10948199
REFERENCE   9  (residues 1 to 1374)
  AUTHORS   Gunther,M., Laithier,M. and Brison,O.
  TITLE     A set of proteins interacting with transcription factor Sp1
            identified in a two-hybrid screening
  JOURNAL   Mol Cell Biochem 210 (1-2), 131-142 (2000)
   PUBMED   10976766
REFERENCE   10 (residues 1 to 1374)
  AUTHORS   Filippov,V., Solovyev,V., Filippova,M. and Gill,S.S.
  TITLE     A novel type of RNase III family proteins in eukaryotes
  JOURNAL   Gene 245 (1), 213-221 (2000)
   PUBMED   10713462
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022417.5, AC106802.3 and
            AC008768.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 13, 2020 this sequence version replaced XP_005248348.1.
            
            Summary: This gene encodes a ribonuclease (RNase) III
            double-stranded RNA-specific ribonuclease and subunit of the
            microprocessor protein complex, which catalyzes the initial
            processing step of microRNA (miRNA) synthesis. The encoded protein
            cleaves the stem loop structure from the primary microRNA
            (pri-miRNA) in the nucleus, yielding the precursor miRNA
            (pre-miRNA), which is then exported to the cytoplasm for further
            processing. In a human cell line lacking a functional copy of this
            gene, canonical miRNA synthesis is reduced. Somatic mutations in
            this gene have been observed in human patients with kidney cancer.
            [provided by RefSeq, Sep 2016].
            
            Transcript Variant: Variants 1 and 3 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.253760.1,
                                           SRR18074969.387240.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344624.8/ ENSP00000339845.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.3"
     Protein         1..1374
                     /product="ribonuclease 3 isoform 1"
                     /EC_number="3.1.26.3"
                     /note="nuclear RNase III Drosha; ribonuclease type III,
                     nuclear; drosha, double-stranded RNA-specific
                     endoribonuclease; putative protein p241 which interacts
                     with transcription factor Sp1; putative ribonuclease III;
                     ribonuclease 3"
                     /calculated_mol_wt=159186
     Region          1..95
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Region          130..406
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Region          220..>278
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     Site            355
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Site            373
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21406692,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Region          390..1365
                     /region_name="Necessary for interaction with DGCR8 and
                     pri-miRNA processing activity.
                     /evidence=ECO:0000269|PubMed:26027739,
                     ECO:0000269|PubMed:26748718"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Region          452..497
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NRR4.2)"
     Region          959..1079
                     /region_name="RIBOc"
                     /note="Ribonuclease III C terminal domain. This group
                     consists of eukaryotic, bacterial and archeal ribonuclease
                     III (RNAse III) proteins. RNAse III is a double stranded
                     RNA-specific endonuclease. Prokaryotic RNAse III is
                     important in post-transcriptional...; cd00593"
                     /db_xref="CDD:238333"
     Site            order(966..967,970..971,974,977..978,981..982,984..986,
                     996,1052,1061)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(966,969,973,1042,1045)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(969,1042,1045)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          1105..1333
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     Site            1215
                     /site_type="other"
                     /note="Important for activity. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9NRR4.2)"
     CDS             1..1374
                     /gene="DROSHA"
                     /gene_synonym="ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L;
                     RNASEN"
                     /coded_by="NM_001382508.1:354..4478"
                     /note="isoform 1 is encoded by transcript variant 3"
                     /db_xref="CCDS:CCDS47195.1"
                     /db_xref="GeneID:29102"
                     /db_xref="HGNC:HGNC:17904"
                     /db_xref="MIM:608828"
ORIGIN      
        1 mmqgntchrm sfhpgrgcpr grgghgarps apsfrpqnlr llhpqqppvq yqyeppsaps
       61 ttfsnspapn flpprpdfvp fpppmppsaq gplppcpirp pfpnhqmrhp fpvppcfppm
      121 pppmpcpnnp pvpgappgqg tfpfmmppps mphpppppvm pqqvnyqypp gyshhnfppp
      181 sfnsfqnnps sflpsannss sphfrhlppy plpkapserr sperlkhydd hrhrdhshgr
      241 gerhrsldrr ergrspdrrr qdsryrsdyd rgrtpsrhrs yersrerere rhrhrdnrrs
      301 pslersykke ykrsgrsygl svvpepagct pelpgeiikn tdswapplei vnhrspsrek
      361 krarweeekd rwsdnqssgk dknytsikek epeetmpdkn eeeeeellkp vwircthsen
      421 yyssdpmdqv gdstvvgtsr lrdlydkfee elgsrqekak aarppweppk tkldedless
      481 sesecesded stcssssdse vfdviaeikr kkahpdrlhd elwyndpgqm ndgplckcsa
      541 karrtgirhs iypgeeaikp crpmtnnagr lfhyritvsp ptnfltdrpt vieyddheyi
      601 fegfsmfaha pltniplckv irfnidytih fieemmpenf cvkglelfsl flfrdilely
      661 dwnlkgplfe dsppccprfh fmprfvrflp dggkevlsmh qillyllrcs kalvpeeeia
      721 nmlqweelew qkyaeeckgm ivtnpgtkps svridqldre qfnpdvitfp iivhfgirpa
      781 qlsyagdpqy qklwksyvkl rhllanspkv kqtdkqklaq reealqkirq kntmrrevtv
      841 elssqgfwkt girsdvcqha mmlpvlthhi ryhqclmhld kligytfqdr cllqlamthp
      901 shhlnfgmnp dharnslsnc girqpkygdr kvhhmhmrkk gintlinims rlgqddptps
      961 rinhnerlef lgdavveflt svhlyylfps leegglatyr taivqnqhla mlakkleldr
     1021 fmlyahgpdl cresdlrham ancfealiga vylegsleea kqlfgrllfn dpdlrevwln
     1081 yplhplqlqe pntdrqliet spvlqkltef eeaigvifth vrllaraftl rtvgfnhltl
     1141 ghnqrmeflg dsimqlvate ylfihfpdhh eghltllrss lvnnrtqakv aeelgmqeya
     1201 itndktkrpv alrtktladl lesfiaalyi dkdleyvhtf mnvcffprlk efilnqdwnd
     1261 pksqlqqccl tlrtegkepd iplyktlqtv gpshartytv avyfkgerig cgkgpsiqqa
     1321 emgaamdale kynfpqmahq krfierkyrq elkemrwere hqerepdete dikk
//
