LOCUS       NM_006442                822 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.
ACCESSION   NM_006442
VERSION     NM_006442.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  AUTHORS   Qin,Y., Huttlin,E.L., Winsnes,C.F., Gosztyla,M.L., Wacheul,L.,
            Kelly,M.R., Blue,S.M., Zheng,F., Chen,M., Schaffer,L.V., Licon,K.,
            Backstrom,A., Vaites,L.P., Lee,J.J., Ouyang,W., Liu,S.N., Zhang,T.,
            Silva,E., Park,J., Pitea,A., Kreisberg,J.F., Gygi,S.P., Ma,J.,
            Harper,J.W., Yeo,G.W., Lafontaine,D.L.J., Lundberg,E. and Ideker,T.
  TITLE     A multi-scale map of cell structure fusing protein images and
            interactions
  JOURNAL   Nature 600 (7889), 536-542 (2021)
   PUBMED   34819669
REFERENCE   3  (bases 1 to 822)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 822)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 822)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 822)
  AUTHORS   Castano,E., Gross,P., Wang,Z., Roeder,R.G. and Oelgeschlager,T.
  TITLE     The C-terminal domain-phosphorylated IIO form of RNA polymerase II
            is associated with the transcription repressor NC2 (Dr1/DRAP1) and
            is required for transcription activation in human nuclear extracts
  JOURNAL   Proc Natl Acad Sci U S A 97 (13), 7184-7189 (2000)
   PUBMED   10852970
REFERENCE   7  (bases 1 to 822)
  AUTHORS   Ikeda,K., Halle,J.P., Stelzer,G., Meisterernst,M. and Kawakami,K.
  TITLE     Involvement of negative cofactor NC2 in active repression by zinc
            finger-homeodomain transcription factor AREB6
  JOURNAL   Mol Cell Biol 18 (1), 10-18 (1998)
   PUBMED   9418848
REFERENCE   8  (bases 1 to 822)
  AUTHORS   Yeung,K., Kim,S. and Reinberg,D.
  TITLE     Functional dissection of a human Dr1-DRAP1 repressor complex
  JOURNAL   Mol Cell Biol 17 (1), 36-45 (1997)
   PUBMED   8972183
REFERENCE   9  (bases 1 to 822)
  AUTHORS   Goppelt,A., Stelzer,G., Lottspeich,F. and Meisterernst,M.
  TITLE     A mechanism for repression of class II gene transcription through
            specific binding of NC2 to TBP-promoter complexes via heterodimeric
            histone fold domains
  JOURNAL   EMBO J 15 (12), 3105-3116 (1996)
   PUBMED   8670811
REFERENCE   10 (bases 1 to 822)
  AUTHORS   Mermelstein,F., Yeung,K., Cao,J., Inostroza,J.A.,
            Erdjument-Bromage,H., Eagelson,K., Landsman,D., Levitt,P.,
            Tempst,P. and Reinberg,D.
  TITLE     Requirement of a corepressor for Dr1-mediated repression of
            transcription
  JOURNAL   Genes Dev 10 (8), 1033-1048 (1996)
   PUBMED   8608938
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG036132.1, BC010025.2 and
            BQ773919.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006442.3.
            
            Summary: Transcriptional repression is a general mechanism for
            regulating transcriptional initiation in organisms ranging from
            yeast to humans.  Accurate initiation of transcription from
            eukaryotic protein-encoding genes requires the assembly of a large
            multiprotein complex consisting of RNA polymerase II and general
            transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a
            repressor that interacts with the TATA-binding protein (TBP) of
            TFIID and prevents the formation of an active transcription complex
            by precluding the entry of TFIIA and/or TFIIB into the
            preinitiation complex. The protein encoded by this gene is a
            corepressor of transcription that interacts with DR1 to enhance
            DR1-mediated repression. The interaction between this corepressor
            and DR1 is required for corepressor function and appears to
            stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.428529.1,
                                           SRR1163658.289450.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312515.7/ ENSP00000307850.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BG036132.1         140-162
            24-795              BC010025.2         1-772
            796-822             BQ773919.1         32-58               c
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..822
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /note="DR1 associated protein 1"
                     /db_xref="GeneID:10589"
                     /db_xref="HGNC:HGNC:3019"
                     /db_xref="MIM:602289"
     exon            1..118
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..694
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /note="negative cofactor 2 alpha; negative co-factor
                     2-alpha"
                     /codon_start=1
                     /product="dr1-associated corepressor"
                     /protein_id="NP_006433.2"
                     /db_xref="CCDS:CCDS8123.1"
                     /db_xref="GeneID:10589"
                     /db_xref="HGNC:HGNC:3019"
                     /db_xref="MIM:602289"
                     /translation="MPSKKKKYNARFPPARIKKIMQTDEEIGKVAAAVPVIISRALEL
                     FLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFLKDLVASVPDMQGDGEDNHM
                     DGDKGARRGRKPGSGGRKNGGMGTKSKDKKLSGTDSEQEDESEDTDTDGEEETSQPPP
                     QASHPSAHFQSPPTPFLPFASTLPLPPAPPGPSAPDEEDEEDYDS"
     exon            119..191
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            192..285
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            286..405
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            406..499
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            500..588
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            589..822
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      782..787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /note="hexamer: ATTAAA"
     regulatory      794..799
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /note="hexamer: AATAAA"
     polyA_site      805
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
                     /note="major polyA site"
     polyA_site      822
                     /gene="DRAP1"
                     /gene_synonym="NC2-alpha"
ORIGIN      
        1 gggcggcgag caggcccggg agccgggagg ctgcgggcgg cggcgctgga cccgacgcgg
       61 cgagagaggc cccgagatgc cgagcaagaa gaagaagtac aacgcgcggt tcccgccggc
      121 gcggatcaag aagatcatgc agacggacga agagattggg aaggtggcgg cggcggtgcc
      181 tgtcatcatc tcccgggcgc tcgagctctt cctagagtcg ctgttgaaga aggcctgcca
      241 ggtgacccag tcgcggaacg cgaagaccat gaccacatcc cacctgaagc agtgcatcga
      301 gctggagcag cagtttgact tcttgaagga cctggtggca tctgttcccg acatgcaggg
      361 ggacggggaa gacaaccaca tggatgggga caagggcgcc cgcaggggcc ggaagccagg
      421 cagcggcggc cggaagaacg gtgggatggg aacgaaaagc aaggacaaga agctgtccgg
      481 gacagactcg gagcaggagg atgaatctga ggacacagat actgatgggg aagaggagac
      541 atcacaaccc ccaccccagg ccagccaccc ctctgcccac tttcagagcc ccccgacacc
      601 cttcctgccc ttcgcctcta ctctgccttt gcccccagcg cccccgggcc cctcagcacc
      661 tgatgaagag gacgaagaag attacgactc ctagcgcctt ctgcccccca gaccatagcc
      721 ccttttagtt ggttttagtt gctctggggg gaggagagaa ggtagagctg ttcttaaatt
      781 tattaaaaaa aaaaataaaa gggaatctca gtgtctgttc ca
//
