LOCUS       NM_130434               7281 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens dipeptidyl peptidase 8 (DPP8), transcript variant 1,
            mRNA.
ACCESSION   NM_130434
VERSION     NM_130434.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Li,K., Zhang,Y., Zhao,W., Wang,R., Li,Y., Wei,L., Wang,L., Chen,X.,
            Chen,Z., Liu,P., Nie,N., Tian,X. and Fu,R.
  TITLE     DPP8/9 inhibition attenuates the TGF-beta1-induced excessive
            deposition of extracellular matrix (ECM) in human mesangial cells
            via Smad and Akt signaling pathways
  JOURNAL   Toxicol Lett 395, 1-10 (2024)
   PUBMED   38458339
  REMARK    GeneRIF: DPP8/9 inhibition attenuates the TGF-beta1-induced
            excessive deposition of extracellular matrix (ECM) in human
            mesangial cells via Smad and Akt signaling pathways.
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Zhang,Y., Li,K., Li,Y., Zhao,W., Wang,L., Chen,Z., Ma,X., Yao,T.,
            Wang,J., Dong,W., Li,X., Tian,X. and Fu,R.
  TITLE     Profibrotic mechanisms of DPP8 and DPP9 highly expressed in the
            proximal renal tubule epithelial cells
  JOURNAL   Pharmacol Res 169, 105630 (2021)
   PUBMED   33932609
  REMARK    GeneRIF: Profibrotic mechanisms of DPP8 and DPP9 highly expressed
            in the proximal renal tubule epithelial cells.
REFERENCE   3  (bases 1 to 7281)
  AUTHORS   Hollingsworth,L.R., Sharif,H., Griswold,A.R., Fontana,P.,
            Mintseris,J., Dagbay,K.B., Paulo,J.A., Gygi,S.P., Bachovchin,D.A.
            and Wu,H.
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome
            activation
  JOURNAL   Nature 592 (7856), 778-783 (2021)
   PUBMED   33731932
REFERENCE   4  (bases 1 to 7281)
  AUTHORS   Huang,M., Zhang,X., Toh,G.A., Gong,Q., Wang,J., Han,Z., Wu,B.,
            Zhong,F. and Chai,J.
  TITLE     Structural and biochemical mechanisms of NLRP1 inhibition by DPP9
  JOURNAL   Nature 592 (7856), 773-777 (2021)
   PUBMED   33731929
REFERENCE   5  (bases 1 to 7281)
  AUTHORS   Suski,M., Wisniewska,A., Kus,K., Kiepura,A., Stachowicz,A.,
            Stachyra,K., Czepiel,K., Madej,J. and Olszanecki,R.
  TITLE     Decrease of the pro-inflammatory M1-like response by inhibition of
            dipeptidyl peptidases 8/9 in THP-1 macrophages - quantitative
            proteomics of the proteome and secretome
  JOURNAL   Mol Immunol 127, 193-202 (2020)
   PUBMED   32998073
  REMARK    GeneRIF: Decrease of the pro-inflammatory M1-like response by
            inhibition of dipeptidyl peptidases 8/9 in THP-1 macrophages -
            quantitative proteomics of the proteome and secretome.
REFERENCE   6  (bases 1 to 7281)
  AUTHORS   Chen,Y.S., Chien,C.H., Goparaju,C.M., Hsu,J.T., Liang,P.H. and
            Chen,X.
  TITLE     Purification and characterization of human prolyl dipeptidase DPP8
            in Sf9 insect cells
  JOURNAL   Protein Expr Purif 35 (1), 142-146 (2004)
   PUBMED   15039077
REFERENCE   7  (bases 1 to 7281)
  AUTHORS   Shu,H., Chen,S., Bi,Q., Mumby,M. and Brekken,D.L.
  TITLE     Identification of phosphoproteins and their phosphorylation sites
            in the WEHI-231 B lymphoma cell line
  JOURNAL   Mol Cell Proteomics 3 (3), 279-286 (2004)
   PUBMED   14729942
REFERENCE   8  (bases 1 to 7281)
  AUTHORS   Qi,S.Y., Riviere,P.J., Trojnar,J., Junien,J.L. and Akinsanya,K.O.
  TITLE     Cloning and characterization of dipeptidyl peptidase 10, a new
            member of an emerging subgroup of serine proteases
  JOURNAL   Biochem J 373 (Pt 1), 179-189 (2003)
   PUBMED   12662155
REFERENCE   9  (bases 1 to 7281)
  AUTHORS   Ajami,K., Abbott,C.A., Obradovic,M., Gysbers,V., Kahne,T.,
            McCaughan,G.W. and Gorrell,M.D.
  TITLE     Structural requirements for catalysis, expression, and dimerization
            in the CD26/DPIV gene family
  JOURNAL   Biochemistry 42 (3), 694-701 (2003)
   PUBMED   12534281
REFERENCE   10 (bases 1 to 7281)
  AUTHORS   Abbott,C.A., Yu,D.M., Woollatt,E., Sutherland,G.R., McCaughan,G.W.
            and Gorrell,M.D.
  TITLE     Cloning, expression and chromosomal localization of a novel human
            dipeptidyl peptidase (DPP) IV homolog, DPP8
  JOURNAL   Eur J Biochem 267 (20), 6140-6150 (2000)
   PUBMED   11012666
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011846.16, AF221634.1,
            AC105129.4 and BU753778.1.
            
            On Nov 22, 2018 this sequence version replaced NM_130434.4.
            
            Summary: This gene encodes a member of the peptidase S9B family, a
            small family of dipeptidyl peptidases that are able to cleave
            peptide substrates at a prolyl bond. The encoded protein shares
            similarity with dipeptidyl peptidase IV in that it is ubiquitously
            expressed, and hydrolyzes the same substrates. These similarities
            suggest that, like dipeptidyl peptidase IV, this protein may play a
            role in T-cell activation and immune function. Alternatively
            spliced transcript variants encoding different isoforms have been
            described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1. Both
            variants 1 and 8 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF221634.1, SRR3476690.698757.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300141.11/ ENSP00000300141.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC011846.16        106807-106808       c
            3-832               AF221634.1         1-830
            833-833             AC011846.16        87080-87080         c
            834-3129            AF221634.1         832-3127
            3130-7230           AC105129.4         39138-43238
            7231-7281           BU753778.1         21-71               c
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..7281
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="dipeptidyl peptidase 8"
                     /db_xref="GeneID:54878"
                     /db_xref="HGNC:HGNC:16490"
                     /db_xref="MIM:606819"
     exon            1..204
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            205..474
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..2864
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /EC_number="3.4.14.5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prolyl dipeptidase DPP8; dipeptidyl peptidase VIII; DPP
                     VIII; dipeptidyl peptidase IV-related protein 1"
                     /codon_start=1
                     /product="dipeptidyl peptidase 8 isoform 1"
                     /protein_id="NP_569118.1"
                     /db_xref="CCDS:CCDS10210.1"
                     /db_xref="GeneID:54878"
                     /db_xref="HGNC:HGNC:16490"
                     /db_xref="MIM:606819"
                     /translation="MAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQ
                     LKKLLADTRKYHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEI
                     PKTINRAAVLMLSWKPLLDLFQATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSG
                     TFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETSCPNIRMDPKLCPADPDWIAFIHSN
                     DIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRYSGYWWCPKAETT
                     PSGGKILRILYEENDESEVEIIHVTSPMLETRRADSFRYPKTGTANPKVTFKMSEIMI
                     DAEGRIIDVIDKELIQPFEILFEGVEYIARAGWTPEGKYAWSILLDRSQTRLQIVLIS
                     PELFIPVEDDVMERQRLIESVPDSVTPLIIYEETTDIWINIHDIFHVFPQSHEEEIEF
                     IFASECKTGFRHLYKITSILKESKYKRSSGGLPAPSDFKCPIKEEIAITSGEWEVLGR
                     HGSNIQVDEVRRLVYFEGTKDSPLEHHLYVVSYVNPGEVTRLTDRGYSHSCCISQHCD
                     FFISKYSNQKNPHCVSLYKLSSPEDDPTCKTKEFWATILDSAGPLPDYTPPEIFSFES
                     TTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYV
                     VVVIDNRGSCHRGLKFEGAFKYKMGQIEIDDQVEGLQYLASRYDFIDLDRVGIHGWSY
                     GGYLSLMALMQRSDIFRVAIAGAPVTLWIFYDTGYTERYMGHPDQNEQGYYLGSVAMQ
                     AEKFPSEPNRLLLLHGFLDENVHFAHTSILLSFLVRAGKPYDLQIYPQERHSIRVPES
                     GEHYELHLLHYLQENLGSRIAALKVI"
     exon            475..587
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            588..761
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            762..930
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1041
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1170
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1232
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1333
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1511
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1671
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1751
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1904
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2040
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2186
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2333
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2486
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2629
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2741
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..7281
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4793..4798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="hexamer: AAGAAA"
     polyA_site      4819
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
     regulatory      7259..7264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="hexamer: AATAAA"
     polyA_site      7281
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgcta aagcctccga ggccaaggcc gctgctactg ccgccgctgc ttcttagtgc
       61 cgcgttcgcc gcctgggttg tcaccggcgc cgccgccgag gaagccactg caaccaggac
      121 cggagtggag gcggcgcagc atgaagcggc gcaggcccgc tccatagcgc acgtcgggac
      181 ggtccgggcg gggccggggg gaaggaaaat gcaacatggc agcagcaatg gaaacagaac
      241 agctgggtgt tgagatattt gaaactgcgg actgtgagga gaatattgaa tcacaggatc
      301 ggcctaaatt ggagcctttt tatgttgagc ggtattcctg gagtcagctt aaaaagctgc
      361 ttgccgatac cagaaaatat catggctaca tgatggctaa ggcaccacat gatttcatgt
      421 ttgtgaagag gaatgatcca gatggacctc attcagacag aatctattac cttgccatgt
      481 ctggtgagaa cagagaaaat acactgtttt attctgaaat tcccaaaact atcaatagag
      541 cagcagtctt aatgctctct tggaagcctc ttttggatct ttttcaggca acactggact
      601 atggaatgta ttctcgagaa gaagaactat taagagaaag aaaacgcatt ggaacagtcg
      661 gaattgcttc ttacgattat caccaaggaa gtggaacatt tctgtttcaa gccggtagtg
      721 gaatttatca cgtaaaagat ggagggccac aaggatttac gcaacaacct ttaaggccca
      781 atctagtgga aactagttgt cccaacatac ggatggatcc aaaattatgc cctgctgatc
      841 cagactggat tgcttttata catagcaacg atatttggat atctaacatc gtaaccagag
      901 aagaaaggag actcacttat gtgcacaatg agctagccaa catggaagaa gatgccagat
      961 cagctggagt cgctaccttt gttctccaag aagaatttga tagatattct ggctattggt
     1021 ggtgtccaaa agctgaaaca actcccagtg gtggtaaaat tcttagaatt ctatatgaag
     1081 aaaatgatga atctgaggtg gaaattattc atgttacatc ccctatgttg gaaacaagga
     1141 gggcagattc attccgttat cctaaaacag gtacagcaaa tcctaaagtc acttttaaga
     1201 tgtcagaaat aatgattgat gctgaaggaa ggatcataga tgtcatagat aaggaactaa
     1261 ttcaaccttt tgagattcta tttgaaggag ttgaatatat tgccagagct ggatggactc
     1321 ctgagggaaa atatgcttgg tccatcctac tagatcgctc ccagactcgc ctacagatag
     1381 tgttgatctc acctgaatta tttatcccag tagaagatga tgttatggaa aggcagagac
     1441 tcattgagtc agtgcctgat tctgtgacgc cactaattat ctatgaagaa acaacagaca
     1501 tctggataaa tatccatgac atctttcatg tttttcccca aagtcacgaa gaggaaattg
     1561 agtttatttt tgcctctgaa tgcaaaacag gtttccgtca tttatacaaa attacatcta
     1621 ttttaaagga aagcaaatat aaacgatcca gtggtgggct gcctgctcca agtgatttca
     1681 agtgtcctat caaagaggag atagcaatta ccagtggtga atgggaagtt cttggccggc
     1741 atggatctaa tatccaagtt gatgaagtca gaaggctggt atattttgaa ggcaccaaag
     1801 actccccttt agagcatcac ctgtacgtag tcagttacgt aaatcctgga gaggtgacaa
     1861 ggctgactga ccgtggctac tcacattctt gctgcatcag tcagcactgt gacttcttta
     1921 taagtaagta tagtaaccag aagaatccac actgtgtgtc cctttacaag ctatcaagtc
     1981 ctgaagatga cccaacttgc aaaacaaagg aattttgggc caccattttg gattcagcag
     2041 gtcctcttcc tgactatact cctccagaaa ttttctcttt tgaaagtact actggattta
     2101 cattgtatgg gatgctctac aagcctcatg atctacagcc tggaaagaaa tatcctactg
     2161 tgctgttcat atatggtggt cctcaggtgc agttggtgaa taatcggttt aaaggagtca
     2221 agtatttccg cttgaatacc ctagcctctc taggttatgt ggttgtagtg atagacaaca
     2281 ggggatcctg tcaccgaggg cttaaatttg aaggcgcctt taaatataaa atgggtcaaa
     2341 tagaaattga cgatcaggtg gaaggactcc aatatctagc ttctcgatat gatttcattg
     2401 acttagatcg tgtgggcatc cacggctggt cctatggagg atacctctcc ctgatggcat
     2461 taatgcagag gtcagatatc ttcagggttg ctattgctgg ggccccagtc actctgtgga
     2521 tcttctatga tacaggatac acggaacgtt atatgggtca ccctgaccag aatgaacagg
     2581 gctattactt aggatctgtg gccatgcaag cagaaaagtt cccctctgaa ccaaatcgtt
     2641 tactgctctt acatggtttc ctggatgaga atgtccattt tgcacatacc agtatattac
     2701 tgagtttttt agtgagggct ggaaagccat atgatttaca gatctatcct caggagagac
     2761 acagcataag agttcctgaa tcgggagaac attatgaact gcatcttttg cactaccttc
     2821 aagaaaacct tggatcacgt attgctgctc taaaagtgat ataattttga cctgtgtaga
     2881 actctctggt atacactggc tatttaacca aatgaggagg tttaatcaac agaaaacaca
     2941 gaattgatca tcacattttg atacctgcca tgtaacatct actcctgaaa ataaatgtgg
     3001 tgccatgcag gggtctacgg tttgtggtag taatctaata ccttaacccc acatgctcaa
     3061 aatcaaatga tacatattcc tgagagaccc agcaatacca taagaattac taaaaaaaaa
     3121 aaaaaaaaaa agacattagc accatgtatt catactaccc tattttcact tttaatagta
     3181 ttataaactt catgaactta attagtgtat ttttacagta tacttttgag tttgttaaaa
     3241 tatgatgata ttagtgattg gtttggttca gttccagaat ctttgactag ttacagattt
     3301 gatagcactt aaatgtaatt gaatagctta tgcttcattg cttgggcata tccagcatgt
     3361 tatgaactaa taactattaa acttgactta accagtcatt cattaataat ttttcaagga
     3421 taacttagtg gcctcctaaa gacacttgtt ttggcactga ccagttttta gccaatttaa
     3481 tctgtatcta gtataaataa ttctcatttt tctttgatga tattaacaga gtgggctttt
     3541 ccttttgcat aaaggctagt aactgtatat gtagcatgga tttaattagt catgatattg
     3601 ataattacag gcagaaaatt tttaatcaaa tgattagagc ttaaatattt gcaggcaagt
     3661 tttttttttt cctttaagaa aaggaaaaag tacacattca ctagaattct tcagaaaatt
     3721 tagtggtgcc agtttccatt tggtatttcc ttattaaaat attctagaat tttaaggaga
     3781 ttgaagggaa tcacagtggg gtggggagac ctgggtttgg ggaatgacag agagaagagg
     3841 tggtgagggc ctgattaaaa actaagcaga agtagtttta acaaaaatac tcatgaaaat
     3901 gtttggaaac tgaaatttaa acaactgtaa tattaaggaa accagaatca ataaatcact
     3961 gtcttgccag cacagctaca gagtaacatg attcagggga ggaaaagttc cttagagtta
     4021 cttttataat tctttttttt tttcctctta ggtttagaaa tcttacaaat ttaaacttta
     4081 tccttttaaa attatttgaa cataatttag atattgtaag cttaaaatac aaatgtttat
     4141 agataacctc tttaccataa actaatccct ggcaagccat ggctctcttt ttttttttgg
     4201 tgtttaaagc ctgtaaacag tttttctgaa tgatcatgaa cttttcttgg tttagcacta
     4261 ggatttagct atgaagagag ctcataggct ttcaggtgct aattgagatc tgccctgtta
     4321 gagtcttggg gtgctagatt ggtcacattg acaccagtgg cagggaaggc atctatgagt
     4381 ttgatgcttt ttatcacaca cttcagtgtt tagaaagtta ttaccaatac ttttaaacaa
     4441 cactccaaga aaatttgcta tatttctttc tcatcactac agagagagta gatttcccca
     4501 tagagagcac agcctccatt agtaaggttg gtgactattg gtaagaggtg gacttcattg
     4561 acaccaagtg ggaggtaggg aaagcccaga aatggcagga tgatatggtg gttctgtcgt
     4621 tgggaaaggt attgggtttt gctgtttgta tttatactgt ataatagata ccacgctttt
     4681 tcttattatc tgtatatgta ttgcttttca tgtttgatat tttcccatgc caagatttgt
     4741 ttatatatat tttcaatgtt aaattaaatt gatttgggta actttcttcc ccaagaaagt
     4801 attttccccc ttaagtatat atctgactgt ggagggtgtt ttgttttgtt ttgttttgtt
     4861 ttgtttggac taactggcct cgaaggatct atccttataa tatatgcaaa gtagaaatgg
     4921 tatacagtag cttttaagca gctactccat tgaaacctct cacccattaa ggctgtttct
     4981 tcaaagtggc atctcttatt ttaccgaaac aaacttagaa aacttcatgt actctggagt
     5041 tcacaggtgt tttccttttg catgttgcct tctcctttcc tcctatcaca aagaatgctt
     5101 ctattatcac tcccagtcac ttaaatgcca ttctgcttct ttagcaggaa aaaatacctg
     5161 aatgaattct cttacagaaa gacagtttga ctgaatttgc tttttacaat cagtgtggaa
     5221 aatatcatag tcaattgagg caatcagtag aaatctcact aaaagtttgt accccctgag
     5281 tatgggtaac agcttaaacg gcaagaatat ataaaagtag caacagttta gtgggagcca
     5341 aaaagcacta ggttaaatat catactacgt ttttaaagac ttgataaatg tgtacacttg
     5401 aacaaccatc cagagggata gttacttgag cattacagag tagtggaact caaaatctaa
     5461 gattcatctg ggatacttct aaaagaaatg tagagttttg aatctcactc ttaaaaatgt
     5521 gtattcccag gctaggcatg gtggctcacg cctgtaatcc tagcactttg ggaggccaag
     5581 gcgggtgaat cacttgaagt caggagatca agaccagctt ggtcgacatg gtgaaacccc
     5641 gtctctacta aaagtacaaa aaaattagct gggcatgatg gcgtgtgcct ataatcccac
     5701 ctactcggga ggctgaggca ggagaatcgc ttgaacctgg gaggcggaag ttgcagtgag
     5761 ccgagatggc gccactgtag tccagtctgg gcgacagagt gagattctgc ctcaaaataa
     5821 ataaataaat aaaaatttgt attccattga tttgggtaga caccaggaat gtgcatttct
     5881 aacaagcttt ccaggcgatc ctatagtaag tcatctgtgg actactttaa gaaactcttc
     5941 tatagagaat ggagttggat taataatagg tgatttttta cactggactg attcacaaga
     6001 acctaaacag tagtccatga agctgctcat ctgtggtaac tatttggccc cgtctcactc
     6061 tgaaagcagc aggagatgtt gtttactttg tttctatccc ctttgtctgg agattaattt
     6121 tggaatgaaa gtttttctct ctatgccatt cctggttctt ttccaaagcc tcatacaaga
     6181 ggattaggtc acaatgcatg cattaccttt taaaagaatg cgatattgat accgatgctt
     6241 actttttttt ttttactact tgttttattc cttccagaaa gtatagcccg cctttctata
     6301 gcatagttct ctttaggtgg aatgattcct ataagatttc tcattattaa atcatgcatt
     6361 tttcaagatg gaatcaatat ttgatttaat ctaagctgat attctcattt gttagaagaa
     6421 caacctacat gctagagaga gaggaggaaa tatacccacg accacacagc cagttagtat
     6481 ccagttggtg ctggactcca gccaggtgtc ctgcctcatg gtagttaaat gatatataga
     6541 aaaggtaaat ttttaaagaa atatttatta atatattcct ataaaacatt ttaaaggtaa
     6601 ccacataaaa atggttaatt tttccattcc aaagtaaatg ctaagcatgt ttattaatga
     6661 agcagtactt ctgattagta tatgacattc tgaagttaat taaactcatt gcactaaatg
     6721 tgtcttcctt ggtatagtgg aggatttgag gattggaata tagagtagag tgcttgctta
     6781 agcctgggag cccatcttta tagctatttg atgtaagaaa agagacatgg ccatttctaa
     6841 actatataag gtgagtgtgt ctattcccag cagatataaa ggaaaaagga aacttttttg
     6901 attcccacct tcccagcctc acctagccat cttccagcct caaatataga gatgttagtg
     6961 caaggtcctg ggctctaggt gatcatttca taagtccttt acagataaag aaaaagtagt
     7021 gtttgtatgt ttgtttttaa gtaaccccaa aacaaattta tattgtattc agcaaaattg
     7081 gaattcaggt gtttaatttt agaacatgaa gtgcctgctg ttttaagcat tgacttgtat
     7141 aaaaagaatt gcatgtctcc agtaagctta tgggttttct catttttagg tatatggctt
     7201 ttaatcatgt aaagtgaaac attagttttc ttgcatttta ttacaggttc tttgttgcaa
     7261 taaagatgct gctgaaatta a
//
