LOCUS       NM_001320876            7386 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens dipeptidyl peptidase 8 (DPP8), transcript variant 8,
            mRNA.
ACCESSION   NM_001320876
VERSION     NM_001320876.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Li,K., Zhang,Y., Zhao,W., Wang,R., Li,Y., Wei,L., Wang,L., Chen,X.,
            Chen,Z., Liu,P., Nie,N., Tian,X. and Fu,R.
  TITLE     DPP8/9 inhibition attenuates the TGF-beta1-induced excessive
            deposition of extracellular matrix (ECM) in human mesangial cells
            via Smad and Akt signaling pathways
  JOURNAL   Toxicol Lett 395, 1-10 (2024)
   PUBMED   38458339
  REMARK    GeneRIF: DPP8/9 inhibition attenuates the TGF-beta1-induced
            excessive deposition of extracellular matrix (ECM) in human
            mesangial cells via Smad and Akt signaling pathways.
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Zhang,Y., Li,K., Li,Y., Zhao,W., Wang,L., Chen,Z., Ma,X., Yao,T.,
            Wang,J., Dong,W., Li,X., Tian,X. and Fu,R.
  TITLE     Profibrotic mechanisms of DPP8 and DPP9 highly expressed in the
            proximal renal tubule epithelial cells
  JOURNAL   Pharmacol Res 169, 105630 (2021)
   PUBMED   33932609
  REMARK    GeneRIF: Profibrotic mechanisms of DPP8 and DPP9 highly expressed
            in the proximal renal tubule epithelial cells.
REFERENCE   3  (bases 1 to 7386)
  AUTHORS   Hollingsworth,L.R., Sharif,H., Griswold,A.R., Fontana,P.,
            Mintseris,J., Dagbay,K.B., Paulo,J.A., Gygi,S.P., Bachovchin,D.A.
            and Wu,H.
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome
            activation
  JOURNAL   Nature 592 (7856), 778-783 (2021)
   PUBMED   33731932
REFERENCE   4  (bases 1 to 7386)
  AUTHORS   Huang,M., Zhang,X., Toh,G.A., Gong,Q., Wang,J., Han,Z., Wu,B.,
            Zhong,F. and Chai,J.
  TITLE     Structural and biochemical mechanisms of NLRP1 inhibition by DPP9
  JOURNAL   Nature 592 (7856), 773-777 (2021)
   PUBMED   33731929
REFERENCE   5  (bases 1 to 7386)
  AUTHORS   Suski,M., Wisniewska,A., Kus,K., Kiepura,A., Stachowicz,A.,
            Stachyra,K., Czepiel,K., Madej,J. and Olszanecki,R.
  TITLE     Decrease of the pro-inflammatory M1-like response by inhibition of
            dipeptidyl peptidases 8/9 in THP-1 macrophages - quantitative
            proteomics of the proteome and secretome
  JOURNAL   Mol Immunol 127, 193-202 (2020)
   PUBMED   32998073
  REMARK    GeneRIF: Decrease of the pro-inflammatory M1-like response by
            inhibition of dipeptidyl peptidases 8/9 in THP-1 macrophages -
            quantitative proteomics of the proteome and secretome.
REFERENCE   6  (bases 1 to 7386)
  AUTHORS   Chen,Y.S., Chien,C.H., Goparaju,C.M., Hsu,J.T., Liang,P.H. and
            Chen,X.
  TITLE     Purification and characterization of human prolyl dipeptidase DPP8
            in Sf9 insect cells
  JOURNAL   Protein Expr Purif 35 (1), 142-146 (2004)
   PUBMED   15039077
REFERENCE   7  (bases 1 to 7386)
  AUTHORS   Shu,H., Chen,S., Bi,Q., Mumby,M. and Brekken,D.L.
  TITLE     Identification of phosphoproteins and their phosphorylation sites
            in the WEHI-231 B lymphoma cell line
  JOURNAL   Mol Cell Proteomics 3 (3), 279-286 (2004)
   PUBMED   14729942
REFERENCE   8  (bases 1 to 7386)
  AUTHORS   Qi,S.Y., Riviere,P.J., Trojnar,J., Junien,J.L. and Akinsanya,K.O.
  TITLE     Cloning and characterization of dipeptidyl peptidase 10, a new
            member of an emerging subgroup of serine proteases
  JOURNAL   Biochem J 373 (Pt 1), 179-189 (2003)
   PUBMED   12662155
REFERENCE   9  (bases 1 to 7386)
  AUTHORS   Ajami,K., Abbott,C.A., Obradovic,M., Gysbers,V., Kahne,T.,
            McCaughan,G.W. and Gorrell,M.D.
  TITLE     Structural requirements for catalysis, expression, and dimerization
            in the CD26/DPIV gene family
  JOURNAL   Biochemistry 42 (3), 694-701 (2003)
   PUBMED   12534281
REFERENCE   10 (bases 1 to 7386)
  AUTHORS   Abbott,C.A., Yu,D.M., Woollatt,E., Sutherland,G.R., McCaughan,G.W.
            and Gorrell,M.D.
  TITLE     Cloning, expression and chromosomal localization of a novel human
            dipeptidyl peptidase (DPP) IV homolog, DPP8
  JOURNAL   Eur J Biochem 267 (20), 6140-6150 (2000)
   PUBMED   11012666
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011846.16, AK296915.1,
            AC105129.4 and BU753778.1.
            
            On May 31, 2019 this sequence version replaced NM_001320876.1.
            
            Summary: This gene encodes a member of the peptidase S9B family, a
            small family of dipeptidyl peptidases that are able to cleave
            peptide substrates at a prolyl bond. The encoded protein shares
            similarity with dipeptidyl peptidase IV in that it is ubiquitously
            expressed, and hydrolyzes the same substrates. These similarities
            suggest that, like dipeptidyl peptidase IV, this protein may play a
            role in T-cell activation and immune function. Alternatively
            spliced transcript variants encoding different isoforms have been
            described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (8) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 8 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK296915.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AC011846.16        106801-106808       c
            9-1265              AK296915.1         4-1260
            1266-1266           AC011846.16        76818-76818         c
            1267-1477           AK296915.1         1262-1472
            1478-1478           AC105129.4         5396-5396
            1479-1817           AK296915.1         1474-1812
            1818-1818           AC105129.4         11509-11509
            1819-2502           AK296915.1         1814-2497
            2503-2503           AC105129.4         31405-31405
            2504-2887           AK296915.1         2499-2882
            2888-2888           AC105129.4         38791-38791
            2889-3010           AK296915.1         2884-3005
            3011-7335           AC105129.4         38914-43238
            7336-7386           BU753778.1         21-71               c
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..7386
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="dipeptidyl peptidase 8"
                     /db_xref="GeneID:54878"
                     /db_xref="HGNC:HGNC:16490"
                     /db_xref="MIM:606819"
     exon            1..309
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            310..579
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     CDS             321..2969
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /EC_number="3.4.14.5"
                     /note="isoform 1 is encoded by transcript variant 8;
                     prolyl dipeptidase DPP8; dipeptidyl peptidase VIII; DPP
                     VIII; dipeptidyl peptidase IV-related protein 1"
                     /codon_start=1
                     /product="dipeptidyl peptidase 8 isoform 1"
                     /protein_id="NP_001307805.1"
                     /db_xref="CCDS:CCDS10210.1"
                     /db_xref="GeneID:54878"
                     /db_xref="HGNC:HGNC:16490"
                     /db_xref="MIM:606819"
                     /translation="MAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQ
                     LKKLLADTRKYHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEI
                     PKTINRAAVLMLSWKPLLDLFQATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSG
                     TFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETSCPNIRMDPKLCPADPDWIAFIHSN
                     DIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRYSGYWWCPKAETT
                     PSGGKILRILYEENDESEVEIIHVTSPMLETRRADSFRYPKTGTANPKVTFKMSEIMI
                     DAEGRIIDVIDKELIQPFEILFEGVEYIARAGWTPEGKYAWSILLDRSQTRLQIVLIS
                     PELFIPVEDDVMERQRLIESVPDSVTPLIIYEETTDIWINIHDIFHVFPQSHEEEIEF
                     IFASECKTGFRHLYKITSILKESKYKRSSGGLPAPSDFKCPIKEEIAITSGEWEVLGR
                     HGSNIQVDEVRRLVYFEGTKDSPLEHHLYVVSYVNPGEVTRLTDRGYSHSCCISQHCD
                     FFISKYSNQKNPHCVSLYKLSSPEDDPTCKTKEFWATILDSAGPLPDYTPPEIFSFES
                     TTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYV
                     VVVIDNRGSCHRGLKFEGAFKYKMGQIEIDDQVEGLQYLASRYDFIDLDRVGIHGWSY
                     GGYLSLMALMQRSDIFRVAIAGAPVTLWIFYDTGYTERYMGHPDQNEQGYYLGSVAMQ
                     AEKFPSEPNRLLLLHGFLDENVHFAHTSILLSFLVRAGKPYDLQIYPQERHSIRVPES
                     GEHYELHLLHYLQENLGSRIAALKVI"
     exon            580..692
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            693..866
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1035
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1146
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1275
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1337
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1438
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1616
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1776
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1856
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2009
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2145
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2291
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2438
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2439..2591
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2734
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..2846
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..7386
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4898..4903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="hexamer: AAGAAA"
     polyA_site      4924
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
     regulatory      7364..7369
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="hexamer: AATAAA"
     polyA_site      7386
                     /gene="DPP8"
                     /gene_synonym="DP8; DPRP-1; DPRP1; MST097; MSTP097;
                     MSTP135; MSTP141"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgcta aagcctccga ggccaaggcc gctgctactg ccgccgctgc ttcttagtgc
       61 cgcgttcgcc gcctgggttg tcaccggcgc cgccgccgag gaagccactg caaccaggac
      121 cggagtggag gcggcgcagc atgaagcggc gcaggcccgc tccatagcgc acgtcgggac
      181 ggtccgggcg gggccggggg gaaggtgcag tatccgcccc cttctccttc tcctgcgccg
      241 gcgaggcctg ggccacggct caccaggccc tttccgcctc acggagggac tgctcggcgc
      301 gttctcaggg aaaatgcaac atggcagcag caatggaaac agaacagctg ggtgttgaga
      361 tatttgaaac tgcggactgt gaggagaata ttgaatcaca ggatcggcct aaattggagc
      421 ctttttatgt tgagcggtat tcctggagtc agcttaaaaa gctgcttgcc gataccagaa
      481 aatatcatgg ctacatgatg gctaaggcac cacatgattt catgtttgtg aagaggaatg
      541 atccagatgg acctcattca gacagaatct attaccttgc catgtctggt gagaacagag
      601 aaaatacact gttttattct gaaattccca aaactatcaa tagagcagca gtcttaatgc
      661 tctcttggaa gcctcttttg gatctttttc aggcaacact ggactatgga atgtattctc
      721 gagaagaaga actattaaga gaaagaaaac gcattggaac agtcggaatt gcttcttacg
      781 attatcacca aggaagtgga acatttctgt ttcaagccgg tagtggaatt tatcacgtaa
      841 aagatggagg gccacaagga tttacgcaac aacctttaag gcccaatcta gtggaaacta
      901 gttgtcccaa catacggatg gatccaaaat tatgccctgc tgatccagac tggattgctt
      961 ttatacatag caacgatatt tggatatcta acatcgtaac cagagaagaa aggagactca
     1021 cttatgtgca caatgagcta gccaacatgg aagaagatgc cagatcagct ggagtcgcta
     1081 cctttgttct ccaagaagaa tttgatagat attctggcta ttggtggtgt ccaaaagctg
     1141 aaacaactcc cagtggtggt aaaattctta gaattctata tgaagaaaat gatgaatctg
     1201 aggtggaaat tattcatgtt acatccccta tgttggaaac aaggagggca gattcattcc
     1261 gttatcctaa aacaggtaca gcaaatccta aagtcacttt taagatgtca gaaataatga
     1321 ttgatgctga aggaaggatc atagatgtca tagataagga actaattcaa ccttttgaga
     1381 ttctatttga aggagttgaa tatattgcca gagctggatg gactcctgag ggaaaatatg
     1441 cttggtccat cctactagat cgctcccaga ctcgcctaca gatagtgttg atctcacctg
     1501 aattatttat cccagtagaa gatgatgtta tggaaaggca gagactcatt gagtcagtgc
     1561 ctgattctgt gacgccacta attatctatg aagaaacaac agacatctgg ataaatatcc
     1621 atgacatctt tcatgttttt ccccaaagtc acgaagagga aattgagttt atttttgcct
     1681 ctgaatgcaa aacaggtttc cgtcatttat acaaaattac atctatttta aaggaaagca
     1741 aatataaacg atccagtggt gggctgcctg ctccaagtga tttcaagtgt cctatcaaag
     1801 aggagatagc aattaccagt ggtgaatggg aagttcttgg ccggcatgga tctaatatcc
     1861 aagttgatga agtcagaagg ctggtatatt ttgaaggcac caaagactcc cctttagagc
     1921 atcacctgta cgtagtcagt tacgtaaatc ctggagaggt gacaaggctg actgaccgtg
     1981 gctactcaca ttcttgctgc atcagtcagc actgtgactt ctttataagt aagtatagta
     2041 accagaagaa tccacactgt gtgtcccttt acaagctatc aagtcctgaa gatgacccaa
     2101 cttgcaaaac aaaggaattt tgggccacca ttttggattc agcaggtcct cttcctgact
     2161 atactcctcc agaaattttc tcttttgaaa gtactactgg atttacattg tatgggatgc
     2221 tctacaagcc tcatgatcta cagcctggaa agaaatatcc tactgtgctg ttcatatatg
     2281 gtggtcctca ggtgcagttg gtgaataatc ggtttaaagg agtcaagtat ttccgcttga
     2341 ataccctagc ctctctaggt tatgtggttg tagtgataga caacagggga tcctgtcacc
     2401 gagggcttaa atttgaaggc gcctttaaat ataaaatggg tcaaatagaa attgacgatc
     2461 aggtggaagg actccaatat ctagcttctc gatatgattt cattgactta gatcgtgtgg
     2521 gcatccacgg ctggtcctat ggaggatacc tctccctgat ggcattaatg cagaggtcag
     2581 atatcttcag ggttgctatt gctggggccc cagtcactct gtggatcttc tatgatacag
     2641 gatacacgga acgttatatg ggtcaccctg accagaatga acagggctat tacttaggat
     2701 ctgtggccat gcaagcagaa aagttcccct ctgaaccaaa tcgtttactg ctcttacatg
     2761 gtttcctgga tgagaatgtc cattttgcac ataccagtat attactgagt tttttagtga
     2821 gggctggaaa gccatatgat ttacagatct atcctcagga gagacacagc ataagagttc
     2881 ctgaatcggg agaacattat gaactgcatc ttttgcacta ccttcaagaa aaccttggat
     2941 cacgtattgc tgctctaaaa gtgatataat tttgacctgt gtagaactct ctggtataca
     3001 ctggctattt aaccaaatga ggaggtttaa tcaacagaaa acacagaatt gatcatcaca
     3061 ttttgatacc tgccatgtaa catctactcc tgaaaataaa tgtggtgcca tgcaggggtc
     3121 tacggtttgt ggtagtaatc taatacctta accccacatg ctcaaaatca aatgatacat
     3181 attcctgaga gacccagcaa taccataaga attactaaaa aaaaaaaaaa aaaaaagaca
     3241 ttagcaccat gtattcatac taccctattt tcacttttaa tagtattata aacttcatga
     3301 acttaattag tgtattttta cagtatactt ttgagtttgt taaaatatga tgatattagt
     3361 gattggtttg gttcagttcc agaatctttg actagttaca gatttgatag cacttaaatg
     3421 taattgaata gcttatgctt cattgcttgg gcatatccag catgttatga actaataact
     3481 attaaacttg acttaaccag tcattcatta ataatttttc aaggataact tagtggcctc
     3541 ctaaagacac ttgttttggc actgaccagt ttttagccaa tttaatctgt atctagtata
     3601 aataattctc atttttcttt gatgatatta acagagtggg cttttccttt tgcataaagg
     3661 ctagtaactg tatatgtagc atggatttaa ttagtcatga tattgataat tacaggcaga
     3721 aaatttttaa tcaaatgatt agagcttaaa tatttgcagg caagtttttt tttttccttt
     3781 aagaaaagga aaaagtacac attcactaga attcttcaga aaatttagtg gtgccagttt
     3841 ccatttggta tttccttatt aaaatattct agaattttaa ggagattgaa gggaatcaca
     3901 gtggggtggg gagacctggg tttggggaat gacagagaga agaggtggtg agggcctgat
     3961 taaaaactaa gcagaagtag ttttaacaaa aatactcatg aaaatgtttg gaaactgaaa
     4021 tttaaacaac tgtaatatta aggaaaccag aatcaataaa tcactgtctt gccagcacag
     4081 ctacagagta acatgattca ggggaggaaa agttccttag agttactttt ataattcttt
     4141 ttttttttcc tcttaggttt agaaatctta caaatttaaa ctttatcctt ttaaaattat
     4201 ttgaacataa tttagatatt gtaagcttaa aatacaaatg tttatagata acctctttac
     4261 cataaactaa tccctggcaa gccatggctc tctttttttt tttggtgttt aaagcctgta
     4321 aacagttttt ctgaatgatc atgaactttt cttggtttag cactaggatt tagctatgaa
     4381 gagagctcat aggctttcag gtgctaattg agatctgccc tgttagagtc ttggggtgct
     4441 agattggtca cattgacacc agtggcaggg aaggcatcta tgagtttgat gctttttatc
     4501 acacacttca gtgtttagaa agttattacc aatactttta aacaacactc caagaaaatt
     4561 tgctatattt ctttctcatc actacagaga gagtagattt ccccatagag agcacagcct
     4621 ccattagtaa ggttggtgac tattggtaag aggtggactt cattgacacc aagtgggagg
     4681 tagggaaagc ccagaaatgg caggatgata tggtggttct gtcgttggga aaggtattgg
     4741 gttttgctgt ttgtatttat actgtataat agataccacg ctttttctta ttatctgtat
     4801 atgtattgct tttcatgttt gatattttcc catgccaaga tttgtttata tatattttca
     4861 atgttaaatt aaattgattt gggtaacttt cttccccaag aaagtatttt cccccttaag
     4921 tatatatctg actgtggagg gtgttttgtt ttgttttgtt ttgttttgtt tggactaact
     4981 ggcctcgaag gatctatcct tataatatat gcaaagtaga aatggtatac agtagctttt
     5041 aagcagctac tccattgaaa cctctcaccc attaaggctg tttcttcaaa gtggcatctc
     5101 ttattttacc gaaacaaact tagaaaactt catgtactct ggagttcaca ggtgttttcc
     5161 ttttgcatgt tgccttctcc tttcctccta tcacaaagaa tgcttctatt atcactccca
     5221 gtcacttaaa tgccattctg cttctttagc aggaaaaaat acctgaatga attctcttac
     5281 agaaagacag tttgactgaa tttgcttttt acaatcagtg tggaaaatat catagtcaat
     5341 tgaggcaatc agtagaaatc tcactaaaag tttgtacccc ctgagtatgg gtaacagctt
     5401 aaacggcaag aatatataaa agtagcaaca gtttagtggg agccaaaaag cactaggtta
     5461 aatatcatac tacgttttta aagacttgat aaatgtgtac acttgaacaa ccatccagag
     5521 ggatagttac ttgagcatta cagagtagtg gaactcaaaa tctaagattc atctgggata
     5581 cttctaaaag aaatgtagag ttttgaatct cactcttaaa aatgtgtatt cccaggctag
     5641 gcatggtggc tcacgcctgt aatcctagca ctttgggagg ccaaggcggg tgaatcactt
     5701 gaagtcagga gatcaagacc agcttggtcg acatggtgaa accccgtctc tactaaaagt
     5761 acaaaaaaat tagctgggca tgatggcgtg tgcctataat cccacctact cgggaggctg
     5821 aggcaggaga atcgcttgaa cctgggaggc ggaagttgca gtgagccgag atggcgccac
     5881 tgtagtccag tctgggcgac agagtgagat tctgcctcaa aataaataaa taaataaaaa
     5941 tttgtattcc attgatttgg gtagacacca ggaatgtgca tttctaacaa gctttccagg
     6001 cgatcctata gtaagtcatc tgtggactac tttaagaaac tcttctatag agaatggagt
     6061 tggattaata ataggtgatt ttttacactg gactgattca caagaaccta aacagtagtc
     6121 catgaagctg ctcatctgtg gtaactattt ggccccgtct cactctgaaa gcagcaggag
     6181 atgttgttta ctttgtttct atcccctttg tctggagatt aattttggaa tgaaagtttt
     6241 tctctctatg ccattcctgg ttcttttcca aagcctcata caagaggatt aggtcacaat
     6301 gcatgcatta ccttttaaaa gaatgcgata ttgataccga tgcttacttt ttttttttta
     6361 ctacttgttt tattccttcc agaaagtata gcccgccttt ctatagcata gttctcttta
     6421 ggtggaatga ttcctataag atttctcatt attaaatcat gcatttttca agatggaatc
     6481 aatatttgat ttaatctaag ctgatattct catttgttag aagaacaacc tacatgctag
     6541 agagagagga ggaaatatac ccacgaccac acagccagtt agtatccagt tggtgctgga
     6601 ctccagccag gtgtcctgcc tcatggtagt taaatgatat atagaaaagg taaattttta
     6661 aagaaatatt tattaatata ttcctataaa acattttaaa ggtaaccaca taaaaatggt
     6721 taatttttcc attccaaagt aaatgctaag catgtttatt aatgaagcag tacttctgat
     6781 tagtatatga cattctgaag ttaattaaac tcattgcact aaatgtgtct tccttggtat
     6841 agtggaggat ttgaggattg gaatatagag tagagtgctt gcttaagcct gggagcccat
     6901 ctttatagct atttgatgta agaaaagaga catggccatt tctaaactat ataaggtgag
     6961 tgtgtctatt cccagcagat ataaaggaaa aaggaaactt ttttgattcc caccttccca
     7021 gcctcaccta gccatcttcc agcctcaaat atagagatgt tagtgcaagg tcctgggctc
     7081 taggtgatca tttcataagt cctttacaga taaagaaaaa gtagtgtttg tatgtttgtt
     7141 tttaagtaac cccaaaacaa atttatattg tattcagcaa aattggaatt caggtgttta
     7201 attttagaac atgaagtgcc tgctgtttta agcattgact tgtataaaaa gaattgcatg
     7261 tctccagtaa gcttatgggt tttctcattt ttaggtatat ggcttttaat catgtaaagt
     7321 gaaacattag ttttcttgca ttttattaca ggttctttgt tgcaataaag atgctgctga
     7381 aattaa
//
