LOCUS       NM_001363540            8353 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens dedicator of cytokinesis 4 (DOCK4), transcript variant
            2, mRNA.
ACCESSION   NM_001363540 XM_006716188
VERSION     NM_001363540.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Wu,C., Wang,C., Xiao,B., Li,S., Sheng,Y., Wang,Q., Tao,J., Zhang,Y.
            and Jiang,X.
  TITLE     Integration analysis of lncRNA and mRNA expression data identifies
            DOCK4 as a potential biomarker for elderly osteoporosis
  JOURNAL   BMC Med Genomics 17 (1), 70 (2024)
   PUBMED   38443923
  REMARK    GeneRIF: Integration analysis of lncRNA and mRNA expression data
            identifies DOCK4 as a potential biomarker for elderly osteoporosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Herbst,C., Bothe,V., Wegler,M., Axer-Schaefer,S.,
            Audebert-Bellanger,S., Gecz,J., Cogne,B., Feldman,H.B.,
            Horn,A.H.C., Hurst,A.C.E., Kelly,M.A., Kruer,M.C., Kurolap,A.,
            Laquerriere,A., Li,M., Mark,P.R., Morawski,M., Nizon,M.,
            Pastinen,T., Polster,T., Saugier-Veber,P., SeSong,J., Sticht,H.,
            Stieler,J.T., Thifffault,I., van Eyk,C.L., Marcorelles,P.,
            Vezain-Mouchard,M., Abou Jamra,R. and Oppermann,H.
  TITLE     Heterozygous loss-of-function variants in DOCK4 cause
            neurodevelopmental delay and microcephaly
  JOURNAL   Hum Genet 143 (3), 455-469 (2024)
   PUBMED   38526744
  REMARK    GeneRIF: Heterozygous loss-of-function variants in DOCK4 cause
            neurodevelopmental delay and microcephaly.
REFERENCE   3  (bases 1 to 8353)
  AUTHORS   Xu,X., He,B., Zeng,J., Yin,J., Wang,X., Luo,X., Liang,C., Luo,S.,
            Yan,H., Xiong,S., Tan,Z., Lv,D., Dai,Z., Lin,Z., Lin,J., Ye,X.,
            Chen,R., Li,Y., Wang,Y., Chen,W., Luo,Z., Li,K. and Ma,G.
  TITLE     Genetic variations in DOCK4 contribute to schizophrenia
            susceptibility in a Chinese cohort: A genetic neuroimaging study
  JOURNAL   Behav Brain Res 443, 114353 (2023)
   PUBMED   36822513
  REMARK    GeneRIF: Genetic variations in DOCK4 contribute to schizophrenia
            susceptibility in a Chinese cohort: A genetic neuroimaging study.
REFERENCE   4  (bases 1 to 8353)
  AUTHORS   Huang,Y., Yang,Y., Zhao,Y., Guo,D., Chen,L., Shi,L. and Xu,G.
  TITLE     DOCK4 regulates ghrelin production in gastric X/A-like cells
  JOURNAL   J Endocrinol Invest 45 (7), 1447-1454 (2022)
   PUBMED   35302184
  REMARK    GeneRIF: DOCK4 regulates ghrelin production in gastric X/A-like
            cells.
REFERENCE   5  (bases 1 to 8353)
  AUTHORS   Qin,Y., Huttlin,E.L., Winsnes,C.F., Gosztyla,M.L., Wacheul,L.,
            Kelly,M.R., Blue,S.M., Zheng,F., Chen,M., Schaffer,L.V., Licon,K.,
            Backstrom,A., Vaites,L.P., Lee,J.J., Ouyang,W., Liu,S.N., Zhang,T.,
            Silva,E., Park,J., Pitea,A., Kreisberg,J.F., Gygi,S.P., Ma,J.,
            Harper,J.W., Yeo,G.W., Lafontaine,D.L.J., Lundberg,E. and Ideker,T.
  TITLE     A multi-scale map of cell structure fusing protein images and
            interactions
  JOURNAL   Nature 600 (7889), 536-542 (2021)
   PUBMED   34819669
REFERENCE   6  (bases 1 to 8353)
  AUTHORS   Hiramoto,K., Negishi,M. and Katoh,H.
  TITLE     Dock4 is regulated by RhoG and promotes Rac-dependent cell
            migration
  JOURNAL   Exp Cell Res 312 (20), 4205-4216 (2006)
   PUBMED   17027967
  REMARK    GeneRIF: Taken together, these results suggest that Dock4 plays an
            important role in the regulation of cell migration through
            activation of Rac1, and that RhoG is a key upstream regulator for
            Dock4.
REFERENCE   7  (bases 1 to 8353)
  AUTHORS   Yan,D., Li,F., Hall,M.L., Sage,C., Hu,W.H., Giallourakis,C.,
            Upadhyay,G., Ouyang,X.M., Du,L.L., Bethea,J.R., Chen,Z.Y.,
            Yajnik,V. and Liu,X.Z.
  TITLE     An isoform of GTPase regulator DOCK4 localizes to the stereocilia
            in the inner ear and binds to harmonin (USH1C)
  JOURNAL   J Mol Biol 357 (3), 755-764 (2006)
   PUBMED   16464467
REFERENCE   8  (bases 1 to 8353)
  AUTHORS   Liang,H., Castro,P.D., Ma,J. and Nagarajan,L.
  TITLE     Finer delineation and transcript map of the 7q31 locus deleted in
            myeloid neoplasms
  JOURNAL   Cancer Genet Cytogenet 162 (2), 151-159 (2005)
   PUBMED   16213364
REFERENCE   9  (bases 1 to 8353)
  AUTHORS   Yajnik,V., Paulding,C., Sordella,R., McClatchey,A.I., Saito,M.,
            Wahrer,D.C., Reynolds,P., Bell,D.W., Lake,R., van den Heuvel,S.,
            Settleman,J. and Haber,D.A.
  TITLE     DOCK4, a GTPase activator, is disrupted during tumorigenesis
  JOURNAL   Cell 112 (5), 673-684 (2003)
   PUBMED   12628187
REFERENCE   10 (bases 1 to 8353)
  AUTHORS   Cote,J.F. and Vuori,K.
  TITLE     Identification of an evolutionarily conserved superfamily of
            DOCK180-related proteins with guanine nucleotide exchange activity
  JOURNAL   J Cell Sci 115 (Pt 24), 4901-4913 (2002)
   PUBMED   12432077
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004111.1, AC003080.1,
            AC003077.1 and AC005047.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 31, 2019 this sequence version replaced NM_001363540.1.
            
            Summary: This gene is a member of the dedicator of cytokinesis
            (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a
            DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated,
            cytoplasmic protein functions as a guanine nucleotide exchange
            factor and is involved in regulation of adherens junctions between
            cells. Mutations in this gene have been associated with ovarian,
            prostate, glioma, and colorectal cancers. Alternatively spliced
            variants which encode different protein isoforms have been
            described, but only one has been fully characterized. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.183757.1,
                                           SRR1660805.74538.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000428084.6/ ENSP00000410746.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-298               AC004111.1         99585-99882         c
            299-382             AC003080.1         172029-172112       c
            383-423             AC003080.1         168475-168515       c
            424-479             AC003080.1         166429-166484       c
            480-576             AC003080.1         162116-162212       c
            577-725             AC003080.1         156996-157144       c
            726-810             AC003080.1         152287-152371       c
            811-962             AC003080.1         145113-145264       c
            963-1044            AC003080.1         113698-113779       c
            1045-1105           AC003080.1         112792-112852       c
            1106-1238           AC003080.1         108091-108223       c
            1239-1327           AC003080.1         103521-103609       c
            1328-1453           AC003080.1         83760-83885         c
            1454-1578           AC003080.1         69659-69783         c
            1579-1741           AC003080.1         68356-68518         c
            1742-1848           AC003080.1         63594-63700         c
            1849-2005           AC003080.1         45012-45168         c
            2006-2103           AC003080.1         40449-40546         c
            2104-2187           AC003080.1         40251-40334         c
            2188-2288           AC003080.1         39972-40072         c
            2289-2370           AC003080.1         37556-37637         c
            2371-2541           AC003080.1         35966-36136         c
            2542-2734           AC003080.1         31354-31546         c
            2735-2862           AC003080.1         14981-15108         c
            2863-2997           AC003080.1         12745-12879         c
            2998-3096           AC003080.1         2570-2668           c
            3097-3191           AC003077.1         88531-88625         c
            3192-3267           AC003077.1         78043-78118         c
            3268-3368           AC003077.1         75470-75570         c
            3369-3427           AC003077.1         74990-75048         c
            3428-3576           AC003077.1         56626-56774         c
            3577-3662           AC003077.1         54831-54916         c
            3663-3689           AC003077.1         50266-50292         c
            3690-3785           AC003077.1         50026-50121         c
            3786-3846           AC003077.1         49033-49093         c
            3847-3940           AC003077.1         44445-44538         c
            3941-4089           AC003077.1         35698-35846         c
            4090-4176           AC003077.1         33201-33287         c
            4177-4281           AC003077.1         31287-31391         c
            4282-4423           AC003077.1         26350-26491         c
            4424-4590           AC003077.1         24793-24959         c
            4591-4677           AC003077.1         21684-21770         c
            4678-4854           AC003077.1         13436-13612         c
            4855-4938           AC003077.1         12503-12586         c
            4939-5058           AC003077.1         8182-8301           c
            5059-5180           AC003077.1         7709-7830           c
            5181-5301           AC003077.1         7263-7383           c
            5302-5383           AC003077.1         5565-5646           c
            5384-5493           AC003077.1         5303-5412           c
            5494-5566           AC003077.1         3086-3158           c
            5567-5680           AC003077.1         1223-1336           c
            5681-5742           AC005047.3         152211-152272       c
            5743-8353           AC005047.3         146095-148705       c
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.1"
     gene            1..8353
                     /gene="DOCK4"
                     /note="dedicator of cytokinesis 4"
                     /db_xref="GeneID:9732"
                     /db_xref="HGNC:HGNC:19192"
                     /db_xref="MIM:607679"
     exon            1..298
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..6189
                     /gene="DOCK4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     dedicator of cytokinesis protein 4"
                     /codon_start=1
                     /product="dedicator of cytokinesis protein 4 isoform 2"
                     /protein_id="NP_001350469.1"
                     /db_xref="CCDS:CCDS87541.1"
                     /db_xref="GeneID:9732"
                     /db_xref="HGNC:HGNC:19192"
                     /db_xref="MIM:607679"
                     /translation="MWIPTEHEKYGVVIASFRGTVPYGLSLEIGDTVQILEKCDGWYR
                     GFALKNPNIKGIFPSSYVHLKNACVKNKGQFEMVIPTEDSVITEMTSTLRDWGTMWKQ
                     LYVRNEGDLFHRLWHIMNEILDLRRQVLVGHLTHDRMKDVKRHITARLDWGNEQLGLD
                     LVPRKEYAMVDPEDISITELYRLMEHRHRKKDTPVQASSHHLFVQMKSLMCSNLGEEL
                     EVIFSLFDSKENRPISERFFLRLNRNGLPKAPDKPERHCSLFVDLGSSELRKDIYITV
                     HIIRIGRMGAGEKKNACSVQYRRPFGCAVLSIADLLTGETKDDLILKVYMCNTESEWY
                     QIHENIIKKLNARYNLTGSNAGLAVSLQLLHGDIEQIRREYSSVFSHGVSITRKLGFS
                     NIIMPGEMRNDLYITIERGEFEKGGKSVARNVEVTMFIVDSSGQTLKDFISFGSGEPP
                     ASEYHSFVLYHNNSPRWSELLKLPIPVDKFRGAHIRFEFRHCSTKEKGEKKLFGFSFV
                     PLMQEDGRTLPDGTHELIVHKCEENTNLQDTTRYLKLPFSKGIFLGNNNQAMKATKES
                     FCITSFLCSTKLTQNGDMLDLLKWRTHPDKITGCLSKLKEIDGSEIVKFLQDTLDTLF
                     GILDENSQKYGSKVFDSLVHIINLLQDSKFHHFKPVMDTYIESHFAGALAYRDLIKVL
                     KWYVDRITEAERQEHIQEVLKAQEYIFKYIVQSRRLFSLATGGQNEEEFRCCIQELLM
                     SVRFFLSQESKGSGALSQSQAVFLSSFPAVYSELLKLFDVREVANLVQDTLGSLPTIL
                     HVDDSLQAIKLQCIGKTVESQLYTNPDSRYILLPVVLHHLHIHLQEQKDLIMCARILS
                     NVFCLIKKNSSEKSVLEEIDVIVASLLDILLRTILEITSRPQPSSSAMRFQFQDVTGE
                     FVACLLSLLRQMTDRHYQQLLDSFNTKEELRDFLLQIFTVFRILIRPEMFPKDWTVMR
                     LVANNVIITTVLYLSDALRKNFLNENFDYKIWDSYFYLAVIFINQLCLQLEMFTPSKK
                     KKVLEKYGDMRVTMGCEIFSMWQNLGEHKLHFIPALIGPFLEVTLIPQPDLRNVMIPI
                     FHDMMDWEQRRSGNFKQVEAKLIDKLDSLMSEGKGDETYRELFNSIIPLFGPYPSLLK
                     KIERETWRESGVSLIATVTRLMERLLDYRDCMKMGEVDGKKIGCTVSLLNFYKTELNK
                     EEMYIRYIHKLYDLHLKAQNFTEAAYTLLLYDELLEWSDRPLREFLTYPMQTEWQRKE
                     HLHLTIIQNFDRGKCWENGIILCRKIAEQYESYYDYRNLSKMRMMEASLYDKIMDQQR
                     LEPEFFRVGFYGKKFPFFLRNKEFVCRGHDYERLEAFQQRMLNEFPHAIAMQHANQPD
                     ETIFQAEAQYLQIYAVTPIPESQEVLQREGVPDNIKSFYKVNHIWKFRYDRPFHKGTK
                     DKENEFKSLWVERTSLYLVQSLPGISRWFEVEKREVVEMSPLENAIEVLENKNQQLKT
                     LISQCQTRQMQNINPLTMCLNGVIDAAVNGGVSRYQEAFFVKEYILSHPEDGEKIARL
                     RELMLEQAQILEFGLAVHEKFVPQDMRPLHKKLVDQFFVMKSSLGIQEFSACMQASPV
                     HFPNGSPRVCRNSAPASVSPDGTRVIPRRSPLSYPAVNRYSSSSLSSQASAEVSNITG
                     QSESSDEVFNMQPSPSTSSLSSTHSASPNVTSSAPSSARASPLLSDKHKHSRENSCLS
                     PRERPCSAIYPTPVEPSQRMLFNHIGDGALPRSDPNLSAPEKAVNPTPSSWSLDSGKE
                     AKNMSDSGKLISPPVPPRPTQTASPARHTTSVSPSPAGRSPLKGSVQSFTPSPVEYHS
                     PGLISNSPVLSGSYSSGISSLSRCSTSETSGFENQVNEQSAPLPVPVPVPVPSYGGEE
                     PVRKESKTPPPYSVYERTLRRPVPLPHSLSIPVTSEPPALPPKPLAARSSHLENGARR
                     TDPGPRPRPLPRKVSQL"
     exon            299..382
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            383..423
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            424..479
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            480..576
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            577..725
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            726..810
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            811..962
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1044
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1105
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1238
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1327
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1453
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1578
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1741
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1848
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..2005
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2103
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2187
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2288
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2370
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2541
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2734
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..2862
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..2997
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3096
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3191
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3192..3267
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3368
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3369..3427
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3576
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3577..3662
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3689
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3690..3785
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3846
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3847..3940
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3941..4089
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4090..4176
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4177..4281
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4282..4423
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4424..4590
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4591..4677
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4678..4854
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4855..4938
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            4939..5058
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5059..5180
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5181..5301
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5302..5383
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5384..5493
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5494..5566
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5567..5680
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5681..5742
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     exon            5743..8353
                     /gene="DOCK4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8329..8334
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DOCK4"
                     /note="hexamer: AATAAA"
     polyA_site      8353
                     /gene="DOCK4"
                     /note="major polyA site"
ORIGIN      
        1 gaatcagaac aatgactgcg ccgccgggta cccgggagcg cgtcggagga ctgtgagcag
       61 ccgccgccgg gagagctgcg ggcggcgcag tgtcaacgct gggctcggga gcctccagcg
      121 cagatcctgc ccagcgcccg cagcccgcgc gaagccgctg ggctccggca ctgcagcgct
      181 gctcggggac tgtgcattgt tgtgagccgg agaaggggcg cggggattac aaagcctgaa
      241 gaccccggag cccctttagc catgtggata cctacggagc acgagaaata cggcgtggtt
      301 attgccagtt tccgaggaac cgttccatat ggcctgtcat tggaaattgg agatacagtt
      361 cagatcctgg agaagtgtga tggctggtac agaggatttg ccttaaaaaa cccaaatatc
      421 aagggtatat ttccttccag ctacgttcac ttgaaaaatg cctgtgtaaa gaacaaagga
      481 caatttgaaa tggttattcc cactgaagac tctgttatca cagaaatgac atcaacatta
      541 agagactggg gaaccatgtg gaaacaactc tatgtgcgta atgaaggcga tctcttccac
      601 cggctgtggc acatcatgaa tgaaatcctg gacctgaggc ggcaggtgct ggtgggccac
      661 ctcacccacg accggatgaa ggacgtgaag cgccacatta ctgcccggct tgactggggc
      721 aatgaacaac tgggactgga cctggtgcct aggaaagagt acgcaatggt ggatccggaa
      781 gacatcagca ttactgagct ctaccgattg atggaacatc gacatcggaa gaaagacacc
      841 ccggtgcagg ccagcagtca ccacctcttt gtccagatga agagcctcat gtgttccaac
      901 ctgggagagg agctggaggt catcttctca ctctttgaca gtaaagagaa ccggccaatc
      961 agtgagagat ttttcttgag gctgaataga aacgggcttc ccaaagcccc tgataaaccg
     1021 gaacgacatt gctccctctt tgtggatttg ggcagcagtg agctaagaaa ggacatttat
     1081 atcaccgtgc acattatccg aatcggtcga atgggagcag gagaaaaaaa gaatgcctgt
     1141 agtgtccagt accgacgacc ctttggctgt gcagttctta gcatcgctga cctgctaaca
     1201 ggagagacaa aggatgacct cattctgaaa gtatacatgt gtaacacaga gagtgagtgg
     1261 taccaaatcc atgagaacat catcaaaaag ctgaatgcac gttataactt gactggctcc
     1321 aatgcaggtt tagcagtttc cttacagcta ttgcacggag acattgaaca aatcagaagg
     1381 gaatattcat cagtattttc tcatggagta tccataacaa ggaagctggg attttcaaat
     1441 attattatgc ctggtgaaat gaggaatgat ttatatatca ctattgaaag gggagaattt
     1501 gagaaaggag ggaagagcgt ggccagaaat gtggaagtta cgatgttcat tgtagacagt
     1561 agtggccaaa ccctgaagga ttttatctcc ttcggctctg gggagccacc agccagtgag
     1621 taccactcct ttgtgcttta ccataacaac agtcccaggt ggtctgaact gctgaaactt
     1681 cccattcctg tggataaatt ccggggtgca cacatccgct tcgagtttcg gcattgttcc
     1741 acaaaggaga aaggagagaa gaagttgttt gggttttctt ttgtccctct gatgcaagaa
     1801 gatggtagga ctcttccaga tggcactcat gagctcatcg tgcataagtg tgaagaaaac
     1861 acaaatcttc aggatactac ccgctacctc aaacttccct tttccaaggg cattttcctt
     1921 gggaataata atcaagccat gaaggccaca aaggagtcct tttgtattac atcttttctc
     1981 tgttccacaa aactcacaca aaatggtgat atgcttgatc ttttgaaatg gagaacccac
     2041 ccagacaaga tcactggctg tctctctaaa ttaaaagaaa ttgatggctc agagatagta
     2101 aagtttctgc aggatacact ggatacctta tttggaattt tagatgaaaa ttcccaaaaa
     2161 tatgggtcta aagtgtttga ttctttggtt cacataataa atttgctgca agatagcaaa
     2221 tttcatcatt ttaaacctgt aatggacact tacattgaga gtcattttgc tggggcactt
     2281 gcatacagag atctcatcaa agtgctcaaa tggtacgtgg accggatcac agaagcagag
     2341 cggcaagagc atatccagga ggtgctgaag gcacaagaat acatttttaa gtatatagtt
     2401 caatctcgaa ggctgttttc ccttgccact ggtgggcaaa acgaagagga gttccgctgc
     2461 tgcattcagg agcttctcat gtcagtccgt ttctttcttt cgcaagagag caaagggtct
     2521 ggagcattat ctcagtcaca ggctgtgttt ctgagctctt tccctgccgt gtactcagaa
     2581 ctgttgaagc tctttgatgt ccgggaagta gccaacttgg tccaggacac cctgggcagt
     2641 ctgccgacca tcctgcatgt ggatgattcc ctgcaggcca tcaaactgca gtgcattggc
     2701 aaaaccgtgg aaagccagct ttataccaac ccagattccc gatacattct tctgcctgtc
     2761 gtgttacatc acctccacat tcacttgcaa gaacagaagg acctgatcat gtgtgcacgt
     2821 atccttagca acgtattttg tcttatcaag aaaaatagct cagaaaaatc tgtgctggag
     2881 gaaatagatg tgatagtggc cagcttgctg gatattctgc tgaggaccat attggagatc
     2941 accagccgac ctcagccatc cagctcagca atgcggttcc agttccagga tgtcactggg
     3001 gagtttgttg cttgtctcct gtccctatta cgacaaatga cagatagaca ttatcaacag
     3061 cttcttgata gttttaatac aaaggaagaa ctaagggatt tcctgctgca gatatttact
     3121 gtgttccgaa tattgatacg cccggagatg tttccaaagg actggactgt tatgcgcttg
     3181 gttgctaaca atgttattat tacaacagtt ctatacctct cagatgcact tcgtaagaac
     3241 ttcttaaatg aaaactttga ttataagatc tgggattcct acttttacct cgcagtcatt
     3301 tttataaacc agttgtgtct gcagttggag atgttcacac cttccaagaa gaaaaaggtg
     3361 ttagaaaagt atggtgacat gcgggtaaca atgggttgtg aaattttcag catgtggcaa
     3421 aacctaggag agcacaagct tcattttatc cctgccctga ttggcccctt cctagaagtg
     3481 accttgatac cccagccaga tcttcggaat gtcatgattc caatttttca tgatatgatg
     3541 gactgggagc agaggcggag tggcaacttt aaacaggtgg aagccaagct aattgacaaa
     3601 ctggatagcc tgatgtcaga aggcaaaggt gacgaaacat accgcgagct cttcaacagt
     3661 ataattccac tatttggtcc ctatcctagt ctactaaaga aaattgagcg ggaaacatgg
     3721 cgggaaagtg gcgtttcatt aattgctact gtaactcgtc taatggagag gttgttagat
     3781 tacagggact gcatgaaaat gggagaggta gatggcaaaa agattggctg cacagttagc
     3841 cttctgaact tctataagac tgaactgaac aaggaggaga tgtatatacg ctacattcac
     3901 aaactctatg atctgcatct caaagcacag aactttacag aagctgcata taccctcctc
     3961 ttatatgacg agctactgga atggtctgat cggcccctca gggagttcct gacctacccc
     4021 atgcaaacag aatggcagcg caaagagcac ctgcacctca ccatcatcca gaactttgac
     4081 agaggcaaat gttgggagaa tggcattatc ttgtgccgga agattgcaga gcagtatgag
     4141 agttattatg actacagaaa cctgagcaag atgcggatga tggaagcctc tttgtatgac
     4201 aaaattatgg accagcaacg tcttgaacca gagttcttca gagttggatt ttatggaaaa
     4261 aaatttccat ttttcttaag aaataaggag tttgtgtgtc gagggcatga ctacgagagg
     4321 ctggaagcct tccaacagag aatgctgaac gagttccccc atgccatcgc catgcagcac
     4381 gccaaccagc ccgatgagac catcttccag gcagaagctc agtatttgca gatatatgct
     4441 gtgactccca ttccagagag ccaggaggtc ctgcagagag agggtgttcc ggacaacatc
     4501 aaaagcttct ataaagtgaa tcacatctgg aaattccgct atgaccgacc atttcacaaa
     4561 ggcacaaaag ataaagagaa tgaattcaag agtctctggg tggagagaac gtcattatac
     4621 ttggtgcaga gtttgcctgg catctctcgc tggtttgaag tggaaaagcg tgaagtggta
     4681 gaaatgagtc ctctggaaaa tgcaattgaa gtgctagaaa ataagaatca gcagctgaag
     4741 actctgatta gtcagtgtca gacaagacag atgcagaata ttaatcccct gactatgtgc
     4801 ctgaatggag ttatagatgc tgcagttaat ggtggcgttt ccaggtatca agaggcattc
     4861 tttgtcaaag aatatatctt aagtcaccct gaagatgggg agaaaattgc acgattaaga
     4921 gagctgatgc ttgagcaggc acagattctg gaatttggtt tggccgtgca tgagaagttt
     4981 gtacctcaag atatgagacc ccttcacaaa aagctggttg accaattctt tgtgatgaag
     5041 tcgagcttag ggatacagga gttctctgct tgtatgcaag ccagtcctgt ccattttcct
     5101 aatggaagcc ctcgtgtgtg tagaaactca gcacctgctt ctgtgagccc agatggtacc
     5161 agggtaattc ctagacgcag cccgttaagt tacccagctg tcaaccgata ttcttcctcc
     5221 tcactgtcct cacaagcttc tgctgaagta agcaatatta cagggcaatc agaaagctct
     5281 gatgaagtct ttaacatgca gccaagtcca tctacctcaa gcttgagttc tactcactcg
     5341 gcttcaccta atgtgacaag ttctgctcca tcgagtgcca gagcttctcc tttgttgtct
     5401 gacaaacaca aacattcccg agaaaactct tgcctgtcac caagagagag accatgcagt
     5461 gccatctatc caacacctgt ggagccttcg cagaggatgc tgtttaatca tattggagac
     5521 ggggccttgc cacgcagtga cccaaatctc tctgcacctg aaaaagctgt gaaccccacc
     5581 cctagcagct ggagcctgga cagtgggaag gaagccaaga acatgtcgga tagtgggaaa
     5641 cttatctctc cccctgtccc tccaagaccc acacagactg cttcaccagc aagacacacg
     5701 acatcagtat ccccctcgcc tgccgggcga tctccattga agggctctgt gcagtctttc
     5761 accccctctc cagtggagta ccactcgcca ggactcatct ccaactcccc tgtcttgtcg
     5821 ggcagctaca gcagtgggat ttcttctctc agccggtgca gcacgtcgga aacctcaggc
     5881 tttgaaaatc aggtgaatga acagtcggcc cccctgccgg tgccagtgcc ggtgcccgtg
     5941 ccgagctacg gcggggagga gccagtgcgc aaggagagca agactccgcc cccgtacagc
     6001 gtctacgagc ggactctgcg gcgccccgtc ccgctacctc acagcctctc catccccgtc
     6061 acgtcggagc cgcccgcgct gccccccaag cctctggcag cgcgatccag ccacctggag
     6121 aatggggccc ggaggactga ccccggcccg cggcccaggc ccctgccccg caaggtctct
     6181 cagttataag tcacttttct atgtacctgc gatgcattct ttgcccgttt acaaaataag
     6241 aagtatgatg agaagacatt tagtgtaggc actttaataa cttactcagc tccttcgatg
     6301 aatggaatta aaacttgctt attaaatatc atgttgcaca atattaaaag ttgctgatct
     6361 aaaacgccag atgttaaatg aagtatggct gaatttcatt aaaacgtttc tcatttggaa
     6421 gtggtaaata gtgataaaga ctccttttgt acctttttat gttcactttt ttttatatag
     6481 tttaatctta aaaccaatac gatattgtca aacgatacaa tgtgtgacaa tgttgtatcg
     6541 tttttactga atacttgata cttggagaaa gcttattaag tcagtgcaca tcctaacaca
     6601 gtggtcctta ttttagaaga cttctgtaaa taaggcaagg tttatcagtg cagatcatca
     6661 gaattaaagt tcaagcaggc gagcaagaca gtatacttaa ggggttgcaa agcttgggac
     6721 tggaaattgt tttgttcttg aaacaaaata cttctttaag gttgcttttg ctgtttgact
     6781 gctgtctaca ttcgtaaaat tctattttgt gaattggtag ctaaatccct tactaccctg
     6841 acaccgtggt atctactgta tttcttttca aggtgcaatt tgcttcagag ttccaatcag
     6901 ctagattaag caagaggctc cagaagaaat gtttacttga attttgcgct tcctttcttg
     6961 atagtttcct atataaaatt tgtcattgaa caagagcaaa tgctgaagta ttaatgaggc
     7021 acaaatgact gtgccccatt agcaagaatt caggaatcaa tacagacagt attaaattaa
     7081 tagcttaagt gaagaaaaaa aaaacttagt gaaaatgtat tagcacgatt aaatggcaaa
     7141 aggacttata aaaggcaagg gcattaactt tcagtcctgc acaaaataaa aaattcctca
     7201 cgactctcca cttttaccag tggagtttgt cttagctgac ctgtcgtctt tctcttgaag
     7261 gaggattgct gtagacttct ctagcttgaa tattgcaaca tagcatctta ggtctagata
     7321 gggatgctaa tgccagttgt agaagtgtga aaaaagcacc ttgtatgtag taatgtattt
     7381 tatatctttg ttttttcttt tactgactgt ttataacact caattgacaa tagatatgaa
     7441 ctgtatttta aatcatactg ttaaatattt tccctctttt gttgggaagc tcattttagt
     7501 ttaaccatgt ttgttttgtt ggtagcttac ctggaaggca gtgaccactt ttttatattc
     7561 tcttaatgaa accattcagc aggtatatgc tgttgaggct ggttatagag gttttctata
     7621 ataaatgttc aagtattttt gtatataact ggttaatttt aataagagat accattatgt
     7681 gtaaaaaaaa gtaaaaataa acgcaaacag ttgttgatgc agtatgattg ttataattat
     7741 gccaaatact ttacgtatgg aaaaagaata tttgtacata tgtgctttta acaattctgc
     7801 catattgact ttacaatttt gaatgtcgga aaaattaata tatgttaaat atttatgttt
     7861 agtgaaagtg ttcataattg agaaaaggaa catatgcatt ttagctttgt atcttgcaag
     7921 ttttgcagtc agaaattttt tgaactagct tttgcttttg ataacacttc gtgtttgtaa
     7981 ccacattcat atatatatac atatatatgt gaagctccat atttctgttg ctttaaagaa
     8041 gtaaaacctt ccatttaaat aagatgacat gcataagata acaaagcttc cttgatttcc
     8101 ttttcctgtg taatttaata gatttgttga ctagtgcttg ggcacattat aaatcagtgt
     8161 tatttgctct tggagccatt ttttaaaaaa aattttggca gtgagcagtt gaatttatct
     8221 tgaatttatc atgtgtgtgt atttctgaag cagctacata gcagaacatt ttaagagatt
     8281 ctgttagccc acatgttcat gttggttgct gctgaatggt aaatattaaa taaaattacc
     8341 agattaatct taa
//
