LOCUS       NM_003586               2013 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens double C2 domain alpha (DOC2A), transcript variant 3,
            mRNA.
ACCESSION   NM_003586
VERSION     NM_003586.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2013)
  AUTHORS   Hu,X., Tang,J., Lan,X. and Mi,X.
  TITLE     Increased expression of DOC2A in human and rat temporal lobe
            epilepsy
  JOURNAL   Epilepsy Res 151, 78-84 (2019)
   PUBMED   30844661
  REMARK    GeneRIF: This study demonstrated that the DOCA2 increase expression
            in temporal lobe in temporal lobe epilepsy.
REFERENCE   4  (bases 1 to 2013)
  AUTHORS   Ramirez,D.M.O., Crawford,D.C., Chanaday,N.L., Trauterman,B.,
            Monteggia,L.M. and Kavalali,E.T.
  TITLE     Loss of Doc2-Dependent Spontaneous Neurotransmission Augments
            Glutamatergic Synaptic Strength
  JOURNAL   J Neurosci 37 (26), 6224-6230 (2017)
   PUBMED   28539418
  REMARK    GeneRIF: this study, members of the Doc2 family of presynaptic
            proteins were eliminated, which caused a reduction in spontaneous
            neurotransmission, whereas action potential-evoked
            neurotransmission remained relatively normal.
REFERENCE   5  (bases 1 to 2013)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 2013)
  AUTHORS   Mochida,S., Orita,S., Sakaguchi,G., Sasaki,T. and Takai,Y.
  TITLE     Role of the Doc2 alpha-Munc13-1 interaction in the neurotransmitter
            release process
  JOURNAL   Proc Natl Acad Sci U S A 95 (19), 11418-11422 (1998)
   PUBMED   9736751
REFERENCE   7  (bases 1 to 2013)
  AUTHORS   Orita,S., Naito,A., Sakaguchi,G., Maeda,M., Igarashi,H., Sasaki,T.
            and Takai,Y.
  TITLE     Physical and functional interactions of Doc2 and Munc13 in
            Ca2+-dependent exocytotic machinery
  JOURNAL   J Biol Chem 272 (26), 16081-16084 (1997)
   PUBMED   9195900
REFERENCE   8  (bases 1 to 2013)
  AUTHORS   Verhage,M., de Vries,K.J., Roshol,H., Burbach,J.P., Gispen,W.H. and
            Sudhof,T.C.
  TITLE     DOC2 proteins in rat brain: complementary distribution and proposed
            function as vesicular adapter proteins in early stages of secretion
  JOURNAL   Neuron 18 (3), 453-461 (1997)
   PUBMED   9115738
REFERENCE   9  (bases 1 to 2013)
  AUTHORS   Sakaguchi,G., Orita,S., Maeda,M., Igarashi,H. and Takai,Y.
  TITLE     Molecular cloning of an isoform of Doc2 having two C2-like domains
  JOURNAL   Biochem Biophys Res Commun 217 (3), 1053-1061 (1995)
   PUBMED   8554557
REFERENCE   10 (bases 1 to 2013)
  AUTHORS   Orita,S., Sasaki,T., Naito,A., Komuro,R., Ohtsuka,T., Maeda,M.,
            Suzuki,H., Igarashi,H. and Takai,Y.
  TITLE     Doc2: a novel brain protein having two repeated C2-like domains
  JOURNAL   Biochem Biophys Res Commun 206 (2), 439-448 (1995)
   PUBMED   7826360
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC055284.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003586.2.
            
            Summary: There are at least two protein isoforms of the Double C2
            protein, namely alpha (DOC2A) and beta (DOC2B), which contain two
            C2-like domains. DOC2A and DOC2B are encoded by different genes;
            these genes are at times confused with the unrelated DAB2 gene
            which was initially named DOC-2. DOC2A is mainly expressed in brain
            and is suggested to be involved in Ca(2+)-dependent
            neurotransmitter release. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (3) lacks an alternate exon and
            uses an alternate splice site in the 5' UTR compared to variant 1.
            Variants 1 through 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC055284.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000350119.9/ ENSP00000340017.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2013              BC055284.1         54-2066
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     gene            1..2013
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /note="double C2 domain alpha"
                     /db_xref="GeneID:8448"
                     /db_xref="HGNC:HGNC:2985"
                     /db_xref="MIM:604567"
     exon            1..125
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            126..400
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1341
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /note="doc2-alpha; double C2-like domains, alpha"
                     /codon_start=1
                     /product="double C2-like domain-containing protein alpha"
                     /protein_id="NP_003577.2"
                     /db_xref="CCDS:CCDS10666.1"
                     /db_xref="GeneID:8448"
                     /db_xref="HGNC:HGNC:2985"
                     /db_xref="MIM:604567"
                     /translation="MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEG
                     GGGGGGEAPAHLVPLALAPPAALLGATTPEDGAEVDSYDSDDATALGTLEFDLLYDRA
                     SCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDL
                     TYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVP
                     LASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRILLSLSYSSRRRGLLVGILRCA
                     HLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATK
                     TLEVTVWDYDIGKSNDFIGGVSLGPGARGEARKHWSDCLQQPDAALERWHTLTSELPP
                     AAGALSSA"
     exon            401..480
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            481..555
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            556..665
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..792
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            793..852
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1016
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1098
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1195
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..2013
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1995..2000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /note="hexamer: AATAAA"
     polyA_site      2013
                     /gene="DOC2A"
                     /gene_synonym="Doc2"
                     /note="major polyA site"
ORIGIN      
        1 gcactttctc ggcgctccct ccctctcggc tcgcacagct cgactccgcg ctctcctcgc
       61 cggccgccgc cgcccagcct gcgcccgccg tccccccagt gcctgggcac ctgttggagg
      121 cagagccagg ggtgctgcat gaggggccgc aggggcgatc gcatgaccat caacatccag
      181 gagcacatgg ccatcaacgt gtgccccggg cccatccggc ccatccgcca gatctctgac
      241 tacttccccc ggggaccagg acctgaaggg ggcggcgggg gcggcgggga ggcccccgcc
      301 catctggtcc ccctggctct ggccccccct gcagccctcc ttggggccac cacgcctgag
      361 gatggtgcgg aggtggacag ctatgactcg gatgatgcca ccgccctagg cacgctggag
      421 tttgaccttc tctacgaccg ggcctcctgc actctgcact gtagcatcct cagggccaag
      481 ggcctcaagc ccatggattt caatggcctc gccgacccct acgtcaagct gcacttgctg
      541 cctggagcct gtaaggccaa taagctaaaa acgaagactc agaggaacac actgaatccc
      601 gtgtggaatg aggacctgac ttacagcggg atcacagatg acgacatcac gcacaaggtg
      661 ctcaggatcg ccgtctgtga tgaggacaag ctgagtcaca atgagtttat tggggagatc
      721 cgcgtgcccc tccgccgcct caagccttcg cagaagaagc attttaacat ctgcctcgag
      781 cgccaggtcc cgctggcgtc cccctcttcc atgtcagcgg cgctgagggg catctcctgt
      841 tatctgaagg agttggagca ggcggagcag gggcaggggc tgctggagga gcgtggccgc
      901 atcctgctga gtctcagcta cagctcgcgg cgccggggac tgctggtagg catcttgcgc
      961 tgcgcccatc tggctgccat ggacgtcaac ggttactcgg acccctacgt caagacgtac
     1021 ctgaggcccg atgtggacaa gaaatccaag cataagacgt gtgtgaagaa gaagactctc
     1081 aacccagaat ttaacgagga gtttttctac gagatagagc tctccactct ggccaccaag
     1141 accctggaag tcaccgtctg ggactatgac attggcaaat ccaatgactt cattggtggc
     1201 gtgtccctgg ggccaggtgc ccgaggcgag gctcggaagc actggagtga ctgcctgcag
     1261 cagccggacg cagccctgga gcgctggcac accctgacca gtgagctgcc ccctgcggcc
     1321 ggggctctgt cctcagcctg agtggacagc agtgtcccgg cacaggccca tcgagccggg
     1381 tccagtaccc aaccttcgca cgagtgtgtt gcacgtttac acagggtggg ctgcccccac
     1441 cctgcactac ctatttttgt gagtctccgt gacccgggtc tgtctgctca tgaggggctg
     1501 cggagttcta tattcacata tgcaaacctc ctgcctgact cgctagtccc tgcaaatatg
     1561 caaacccccc atcatctgca cacccgggca gtgctcagag cccggcccca ggcccccgcg
     1621 ctcctcactc ctgcctctcc acgctgcccc gtccctctcc cccaacaggg aggaggtcgg
     1681 attaggggag gttcagagga ggagaatgtc tcaaaaaaaa aggacaaaaa acaaacccag
     1741 agccactcct ttaatacaga aacttcattg aaaaaaccta gccctgtaca gctgcccttt
     1801 taaagaggtg gccattgcag ggtctctgcc tccccagcag gtggcagatg ctcccggcca
     1861 ctcgtttaaa taactgtccc ctggaggggg ccggggtttg aaggcagggc ccctgccacc
     1921 ctcctggggg acacccgtgc atgtttacgc cttttctggt tgtgtcagtg gccggagcat
     1981 ggcagctggg catcaataaa cacctccgag gaa
//
