LOCUS       NM_006443                654 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 (DNPH1),
            transcript variant 1, mRNA.
ACCESSION   NM_006443
VERSION     NM_006443.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Carberry,A.E., Devi,S., Harrison,D.J. and da Silva,R.G.
  TITLE     Human 2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1: The Catalytic
            Roles of Tyr24 and Asp80
  JOURNAL   Chembiochem 25 (7), e202400047 (2024)
   PUBMED   38350003
  REMARK    GeneRIF: Human 2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1: The
            Catalytic Roles of Tyr24 and Asp80.
REFERENCE   2  (bases 1 to 654)
  AUTHORS   Wagner,A.G., Eskandari,R. and Schramm,V.L.
  TITLE     An enzyme-coupled microplate assay for activity and inhibition of
            hmdUMP hydrolysis by DNPH1
  JOURNAL   Anal Biochem 672, 115171 (2023)
   PUBMED   37142196
REFERENCE   3  (bases 1 to 654)
  AUTHORS   Fugger,K., Bajrami,I., Silva Dos Santos,M., Young,S.J.,
            Kunzelmann,S., Kelly,G., Hewitt,G., Patel,H., Goldstone,R.,
            Carell,T., Boulton,S.J., MacRae,J., Taylor,I.A. and West,S.C.
  TITLE     Targeting the nucleotide salvage factor DNPH1 sensitizes
            BRCA-deficient cells to PARP inhibitors
  JOURNAL   Science 372 (6538), 156-165 (2021)
   PUBMED   33833118
  REMARK    GeneRIF: Targeting the nucleotide salvage factor DNPH1 sensitizes
            BRCA-deficient cells to PARP inhibitors.
REFERENCE   4  (bases 1 to 654)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 654)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 654)
  AUTHORS   Shin,S., Bosc,D.G., Ingle,J.N., Spelsberg,T.C. and Janknecht,R.
  TITLE     Rcl is a novel ETV1/ER81 target gene upregulated in breast tumors
  JOURNAL   J Cell Biochem 105 (3), 866-874 (2008)
   PUBMED   18726892
  REMARK    GeneRIF: characterization of the human Rcl gene, we cloned its
            promoter, Rcl is a bona fide target gene of ETV1.
REFERENCE   7  (bases 1 to 654)
  AUTHORS   Ghiorghi,Y.K., Zeller,K.I., Dang,C.V. and Kaminski,P.A.
  TITLE     The c-Myc target gene Rcl (C6orf108) encodes a novel enzyme,
            deoxynucleoside 5'-monophosphate N-glycosidase
  JOURNAL   J Biol Chem 282 (11), 8150-8156 (2007)
   PUBMED   17234634
REFERENCE   8  (bases 1 to 654)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   9  (bases 1 to 654)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 654)
  AUTHORS   Lewis,B.C., Shim,H., Li,Q., Wu,C.S., Lee,L.A., Maity,A. and
            Dang,C.V.
  TITLE     Identification of putative c-Myc-responsive genes: characterization
            of rcl, a novel growth-related gene
  JOURNAL   Mol Cell Biol 17 (9), 4967-4978 (1997)
   PUBMED   9271375
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133375.25, BM747628.1 and
            BC011683.2.
            
            On Jun 2, 2019 this sequence version replaced NM_006443.2.
            
            Summary: This gene was identified on the basis of its stimulation
            by c-Myc protein. The latter is a transcription factor that
            participates in the regulation of cell proliferation,
            differentiation, and apoptosis. The exact function of this gene is
            not known but studies in rat suggest a role in cellular
            proliferation and c-Myc-mediated transformation. Two alternative
            transcripts encoding different proteins have been described.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU585603.1, BC011683.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2153946 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000230431.11/ ENSP00000230431.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AL133375.25        51761-51768         c
            9-439               BM747628.1         9-439
            440-654             BC011683.2         426-640
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..654
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /note="2'-deoxynucleoside 5'-phosphate N-hydrolase 1"
                     /db_xref="GeneID:10591"
                     /db_xref="HGNC:HGNC:21218"
                     /db_xref="MIM:618762"
     exon            1..221
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..550
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative c-Myc-responsive; deoxyribonucleoside
                     5'-monophosphate N-glycosidase; c-Myc-responsive protein
                     RCL; 5-hydroxymethyl-dUMP N-hydrolase"
                     /codon_start=1
                     /product="5-hydroxymethyl-dUMP N-hydrolase isoform 1"
                     /protein_id="NP_006434.1"
                     /db_xref="CCDS:CCDS4891.1"
                     /db_xref="GeneID:10591"
                     /db_xref="HGNC:HGNC:21218"
                     /db_xref="MIM:618762"
                     /translation="MAAAMVPGRSESWERGEPGRPALYFCGSIRGGREDRTLYERIVS
                     RLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPSLGVG
                     YELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEA
                     DPPGQVAASPDPTT"
     exon            222..290
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /inference="alignment:Splign:2.1.0"
     exon            291..401
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /inference="alignment:Splign:2.1.0"
     exon            402..654
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      629..634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /note="hexamer: ATTAAA"
     polyA_site      654
                     /gene="DNPH1"
                     /gene_synonym="C6orf108; dJ330M21.3; RCL"
                     /note="major polyA site"
ORIGIN      
        1 gccggagagc gcgggcggct ggggaatggc tgctgccatg gtgccggggc gcagcgagag
       61 ctgggagcgc ggggagcctg gccgcccggc cctgtacttc tgcgggagca ttcgcggcgg
      121 acgcgaggac aggacgctgt acgagcggat cgtgtctcgg ctgcggcgat tcgggacagt
      181 gctcaccgag cacgtggcgg ccgccgagct gggcgcgcgc ggggaagagg ctgctggggg
      241 tgacaggctc atccatgagc aggacctgga gtggctgcag caggcggacg tggtcgtggc
      301 agaagtgaca cagccatcct tgggtgtagg ctatgagctg ggccgggccg tggcctttaa
      361 caagcggatc ctgtgcctgt tccgcccgca gtctggccgc gtgctttcgg ccatgatccg
      421 gggagcagca gatggctctc ggttccaggt gtgggactat gaggagggag aggtggaggc
      481 cctgctggat cgatacttcg aggctgatcc tccagggcag gtggctgcct cccctgaccc
      541 aaccacttga cttaatctca ctttcttaaa ttcttctatt ctcagacact gctctagtac
      601 cattccttcc tcttagcccc aggagcaaat taaaaggtac agttaaaatc ctaa
//
