LOCUS       NM_022552               9421 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens DNA methyltransferase 3 alpha (DNMT3A), transcript
            variant 3, mRNA.
ACCESSION   NM_022552
VERSION     NM_022552.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Zhao,Y., Zheng,Y., Fu,J., Zhang,J., Shao,H., Liu,S., Lai,J.,
            Zhou,X., Liang,R., Jia,L., Cui,W., Yang,J., Wu,C. and Wang,L.
  TITLE     KDM1A, a potent and selective target, for the treatment of
            DNMT3A-deficient non-small cell lung cancer
  JOURNAL   Br J Cancer 131 (4), 655-667 (2024)
   PUBMED   38951697
  REMARK    GeneRIF: KDM1A, a potent and selective target, for the treatment of
            DNMT3A-deficient non-small cell lung cancer.
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Gretarsson,K.H., Abini-Agbomson,S., Gloor,S.L., Weinberg,D.N.,
            McCuiston,J.L., Kumary,V.U.S., Hickman,A.R., Sahu,V., Lee,R.,
            Xu,X., Lipieta,N., Flashner,S., Adeleke,O.A., Popova,I.K.,
            Taylor,H.F., Noll,K., Windham,C.L., Maryanski,D.N., Venters,B.J.,
            Nakagawa,H., Keogh,M.C., Armache,K.J. and Lu,C.
  TITLE     Cancer-associated DNA hypermethylation of Polycomb targets requires
            DNMT3A dual recognition of histone H2AK119 ubiquitination and the
            nucleosome acidic patch
  JOURNAL   Sci Adv 10 (35), eadp0975 (2024)
   PUBMED   39196936
  REMARK    GeneRIF: Cancer-associated DNA hypermethylation of Polycomb targets
            requires DNMT3A dual recognition of histone H2AK119 ubiquitination
            and the nucleosome acidic patch.
REFERENCE   3  (bases 1 to 9421)
  AUTHORS   Zhou,J., Wu,T. and Liu,W.
  TITLE     [Research progress on DNMT3A gene expression in Acute myeloid
            leukemia]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 41 (8), 1010-1015 (2024)
   PUBMED   39097288
  REMARK    GeneRIF: [Research progress on DNMT3A gene expression in Acute
            myeloid leukemia].
            Review article
REFERENCE   4  (bases 1 to 9421)
  AUTHORS   Zebrauskiene,D., Sadauskiene,E., Dapkunas,J., Kairys,V.,
            Balciunas,J., Konovalovas,A., Masiuliene,R., Petraityte,G.,
            Valeviciene,N., Mataciunas,M., Barysiene,J., Mikstiene,V.,
            Tomkuviene,M. and Preiksaitiene,E.
  TITLE     Aortic disease and cardiomyopathy in patients with a novel DNMT3A
            gene variant causing Tatton-Brown-Rahman syndrome
  JOURNAL   Clin Epigenetics 16 (1), 76 (2024)
   PUBMED   38845031
  REMARK    GeneRIF: Aortic disease and cardiomyopathy in patients with a novel
            DNMT3A gene variant causing Tatton-Brown-Rahman syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9421)
  AUTHORS   Wang,M., Zhao,H.Y., Li,D.Q. and Chen,P.
  TITLE     [Relationship between DTA Mutations and Thromboembolism in Patients
            with Myeloproliferative Neoplasms]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 32 (3), 819-824 (2024)
   PUBMED   38926973
  REMARK    GeneRIF: [Relationship between DTA Mutations and Thromboembolism in
            Patients with Myeloproliferative Neoplasms].
REFERENCE   6  (bases 1 to 9421)
  AUTHORS   Fuks,F., Burgers,W.A., Godin,N., Kasai,M. and Kouzarides,T.
  TITLE     Dnmt3a binds deacetylases and is recruited by a sequence-specific
            repressor to silence transcription
  JOURNAL   EMBO J 20 (10), 2536-2544 (2001)
   PUBMED   11350943
REFERENCE   7  (bases 1 to 9421)
  AUTHORS   Xie,S., Wang,Z., Okano,M., Nogami,M., Li,Y., He,W.W., Okumura,K.
            and Li,E.
  TITLE     Cloning, expression and chromosome locations of the human DNMT3
            gene family
  JOURNAL   Gene 236 (1), 87-95 (1999)
   PUBMED   10433969
REFERENCE   8  (bases 1 to 9421)
  AUTHORS   Robertson,K.D., Uzvolgyi,E., Liang,G., Talmadge,C., Sumegi,J.,
            Gonzales,F.A. and Jones,P.A.
  TITLE     The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate
            mRNA expression in normal tissues and overexpression in tumors
  JOURNAL   Nucleic Acids Res 27 (11), 2291-2298 (1999)
   PUBMED   10325416
REFERENCE   9  (bases 1 to 9421)
  AUTHORS   Okano,M., Xie,S. and Li,E.
  TITLE     Cloning and characterization of a family of novel mammalian DNA
            (cytosine-5) methyltransferases
  JOURNAL   Nat Genet 19 (3), 219-220 (1998)
   PUBMED   9662389
REFERENCE   10 (bases 1 to 9421)
  AUTHORS   Ostrowski,P.J. and Tatton-Brown,K.
  TITLE     Tatton-Brown-Rahman Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   35771960
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012074.9.
            
            On Feb 25, 2020 this sequence version replaced NM_022552.4.
            
            Summary: CpG methylation is an epigenetic modification that is
            important for embryonic development, imprinting, and X-chromosome
            inactivation. Studies in mice have demonstrated that DNA
            methylation is required for mammalian development. This gene
            encodes a DNA methyltransferase that is thought to function in de
            novo methylation, rather than maintenance methylation. The protein
            localizes to the cytoplasm and nucleus and its expression is
            developmentally regulated. [provided by RefSeq, Mar 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043617.1, AF067972.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321117.10/ ENSP00000324375.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC012074.9         129334-129433       c
            101-349             AC012074.9         101421-101669       c
            350-454             AC012074.9         87647-87751         c
            455-725             AC012074.9         69949-70219         c
            726-769             AC012074.9         63008-63051         c
            770-916             AC012074.9         62449-62595         c
            917-1132            AC012074.9         35545-35760         c
            1133-1291           AC012074.9         35099-35257         c
            1292-1399           AC012074.9         34559-34666         c
            1400-1556           AC012074.9         34128-34284         c
            1557-1706           AC012074.9         33668-33817         c
            1707-1751           AC012074.9         33528-33572         c
            1752-1831           AC012074.9         32761-32840         c
            1832-1944           AC012074.9         32048-32160         c
            1945-2128           AC012074.9         31663-31846         c
            2129-2213           AC012074.9         31406-31490         c
            2214-2359           AC012074.9         29070-29215         c
            2360-2450           AC012074.9         28148-28238         c
            2451-2599           AC012074.9         27810-27958         c
            2600-2685           AC012074.9         26638-26723         c
            2686-2755           AC012074.9         24444-24513         c
            2756-2874           AC012074.9         23215-23333         c
            2875-9421           AC012074.9         15382-21928         c
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..9421
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /note="DNA methyltransferase 3 alpha"
                     /db_xref="GeneID:1788"
                     /db_xref="HGNC:HGNC:2978"
                     /db_xref="MIM:602769"
     exon            1..100
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            101..349
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..3016
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /EC_number="2.1.1.37"
                     /note="isoform a is encoded by transcript variant 3; DNA
                     MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNA
                     (cytosine-5-)-methyltransferase 3 alpha; DNA
                     (cytosine-5)-methyltransferase 3A; cysteine
                     methyltransferase DNMT3A"
                     /codon_start=1
                     /product="DNA (cytosine-5)-methyltransferase 3A isoform a"
                     /protein_id="NP_072046.2"
                     /db_xref="CCDS:CCDS33157.1"
                     /db_xref="GeneID:1788"
                     /db_xref="HGNC:HGNC:2978"
                     /db_xref="MIM:602769"
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTT
                     ARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPE
                     EGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIES
                     MKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKA
                     KVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATK
                     AGDDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDG
                     KFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDE
                     SDTAKAVEVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAP
                     PPPAKKPRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPL
                     FVGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLL
                     VGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYP
                     PVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIM
                     YVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHD
                     ARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNL
                     PGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKE
                     DILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     exon            350..454
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            455..725
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            726..769
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            770..916
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1132
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1291
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1399
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1556
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1706
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1751
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1831
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1944
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2128
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2213
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2359
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2450
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2599
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2685
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2755
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2756..2874
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2875..9421
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4297..4302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /note="hexamer: AATAAA"
     polyA_site      4319
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /note="major polyA site"
     regulatory      9389..9394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /note="hexamer: AATAAA"
     polyA_site      9421
                     /gene="DNMT3A"
                     /gene_synonym="DNMT3A2; HESJAS; M.HsaIIIA; TBRS"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgggcgg cggcggcggc gagagcagag gacgagccgg gacgcggcgc cgcggcacca
       61 gggcgcgcag ccgggccggc ccgaccccac cggccatacg gtggagccat cgaagccccc
      121 acccacaggc tgacagaggc accgttcacc agagggctca acaccgggat ctatgtttaa
      181 gttttaactc tcgcctccaa agaccacgat aattccttcc ccaaagccca gcagcccccc
      241 agccccgcgc agccccagcc tgcctcccgg cgcccagatg cccgccatgc cctccagcgg
      301 ccccggggac accagcagct ctgctgcgga gcgggaggag gaccgaaagg acggagagga
      361 gcaggaggag ccgcgtggca aggaggagcg ccaagagccc agcaccacgg cacggaaggt
      421 ggggcggcct gggaggaagc gcaagcaccc cccggtggaa agcggtgaca cgccaaagga
      481 ccctgcggtg atctccaagt ccccatccat ggcccaggac tcaggcgcct cagagctatt
      541 acccaatggg gacttggaga agcggagtga gccccagcca gaggagggga gccctgctgg
      601 ggggcagaag ggcggggccc cagcagaggg agagggtgca gctgagaccc tgcctgaagc
      661 ctcaagagca gtggaaaatg gctgctgcac ccccaaggag ggccgaggag cccctgcaga
      721 agcgggcaaa gaacagaagg agaccaacat cgaatccatg aaaatggagg gctcccgggg
      781 ccggctgcgg ggtggcttgg gctgggagtc cagcctccgt cagcggccca tgccgaggct
      841 caccttccag gcgggggacc cctactacat cagcaagcgc aagcgggacg agtggctggc
      901 acgctggaaa agggaggctg agaagaaagc caaggtcatt gcaggaatga atgctgtgga
      961 agaaaaccag gggcccgggg agtctcagaa ggtggaggag gccagccctc ctgctgtgca
     1021 gcagcccact gaccccgcat cccccactgt ggctaccacg cctgagcccg tggggtccga
     1081 tgctggggac aagaatgcca ccaaagcagg cgatgacgag ccagagtacg aggacggccg
     1141 gggctttggc attggggagc tggtgtgggg gaaactgcgg ggcttctcct ggtggccagg
     1201 ccgcattgtg tcttggtgga tgacgggccg gagccgagca gctgaaggca cccgctgggt
     1261 catgtggttc ggagacggca aattctcagt ggtgtgtgtt gagaagctga tgccgctgag
     1321 ctcgttttgc agtgcgttcc accaggccac gtacaacaag cagcccatgt accgcaaagc
     1381 catctacgag gtcctgcagg tggccagcag ccgcgcgggg aagctgttcc cggtgtgcca
     1441 cgacagcgat gagagtgaca ctgccaaggc cgtggaggtg cagaacaagc ccatgattga
     1501 atgggccctg gggggcttcc agccttctgg ccctaagggc ctggagccac cagaagaaga
     1561 gaagaatccc tacaaagaag tgtacacgga catgtgggtg gaacctgagg cagctgccta
     1621 cgcaccacct ccaccagcca aaaagccccg gaagagcaca gcggagaagc ccaaggtcaa
     1681 ggagattatt gatgagcgca caagagagcg gctggtgtac gaggtgcggc agaagtgccg
     1741 gaacattgag gacatctgca tctcctgtgg gagcctcaat gttaccctgg aacaccccct
     1801 cttcgttgga ggaatgtgcc aaaactgcaa gaactgcttt ctggagtgtg cgtaccagta
     1861 cgacgacgac ggctaccagt cctactgcac catctgctgt gggggccgtg aggtgctcat
     1921 gtgcggaaac aacaactgct gcaggtgctt ttgcgtggag tgtgtggacc tcttggtggg
     1981 gccgggggct gcccaggcag ccattaagga agacccctgg aactgctaca tgtgcgggca
     2041 caagggtacc tacgggctgc tgcggcggcg agaggactgg ccctcccggc tccagatgtt
     2101 cttcgctaat aaccacgacc aggaatttga ccctccaaag gtttacccac ctgtcccagc
     2161 tgagaagagg aagcccatcc gggtgctgtc tctctttgat ggaatcgcta cagggctcct
     2221 ggtgctgaag gacttgggca ttcaggtgga ccgctacatt gcctcggagg tgtgtgagga
     2281 ctccatcacg gtgggcatgg tgcggcacca ggggaagatc atgtacgtcg gggacgtccg
     2341 cagcgtcaca cagaagcata tccaggagtg gggcccattc gatctggtga ttgggggcag
     2401 tccctgcaat gacctctcca tcgtcaaccc tgctcgcaag ggcctctacg agggcactgg
     2461 ccggctcttc tttgagttct accgcctcct gcatgatgcg cggcccaagg agggagatga
     2521 tcgccccttc ttctggctct ttgagaatgt ggtggccatg ggcgttagtg acaagaggga
     2581 catctcgcga tttctcgagt ccaaccctgt gatgattgat gccaaagaag tgtcagctgc
     2641 acacagggcc cgctacttct ggggtaacct tcccggtatg aacaggccgt tggcatccac
     2701 tgtgaatgat aagctggagc tgcaggagtg tctggagcat ggcaggatag ccaagttcag
     2761 caaagtgagg accattacta cgaggtcaaa ctccataaag cagggcaaag accagcattt
     2821 tcctgtcttc atgaatgaga aagaggacat cttatggtgc actgaaatgg aaagggtatt
     2881 tggtttccca gtccactata ctgacgtctc caacatgagc cgcttggcga ggcagagact
     2941 gctgggccgg tcatggagcg tgccagtcat ccgccacctc ttcgctccgc tgaaggagta
     3001 ttttgcgtgt gtgtaaggga catgggggca aactgaggta gcgacacaaa gttaaacaaa
     3061 caaacaaaaa acacaaaaca taataaaaca ccaagaacat gaggatggag agaagtatca
     3121 gcacccagaa gagaaaaagg aatttaaaac aaaaaccaca gaggcggaaa taccggaggg
     3181 ctttgccttg cgaaaagggt tggacatcat ctcctgattt ttcaatgtta ttcttcagtc
     3241 ctatttaaaa acaaaaccaa gctcccttcc cttcctcccc cttccctttt ttttcggtca
     3301 gaccttttat tttctactct tttcagaggg gttttctgtt tgtttgggtt ttgtttcttg
     3361 ctgtgactga aacaagaagg ttattgcagc aaaaatcagt aacaaaaaat agtaacaata
     3421 ccttgcagag gaaaggtggg agagaggaaa aaaggaaatt ctatagaaat ctatatattg
     3481 ggttgttttt ttttttgttt tttgtttttt ttttttgggt tttttttttt actatatatc
     3541 ttttttttgt tgtctctagc ctgatcagat aggagcacaa gcaggggacg gaaagagaga
     3601 gacactcagg cggcagcatt ccctcccagc cactgagctg tcgtgccagc accattcctg
     3661 gtcacgcaaa acagaaccca gttagcagca gggagacgag aacaccacac aagacatttt
     3721 tctacagtat ttcaggtgcc taccacacag gaaaccttga agaaaatcag tttctagaag
     3781 ccgctgttac ctcttgttta cagtttatat atatatgata gatatgagat atatatataa
     3841 aaggtactgt taactactgt acaacccgac ttcataatgg tgctttcaaa cagcgagatg
     3901 agtaaaaaca tcagcttcca cgttgccttc tgcgcaaagg gtttcaccaa ggatggagaa
     3961 agggagacag cttgcagatg gcgcgttctc acggtgggct cttccccttg gtttgtaacg
     4021 aagtgaagga ggagaacttg ggagccaggt tctccctgcc aaaaaggggg ctagatgagg
     4081 tggtcgggcc cgtggacagc tgagagtggg attcatccag actcatgcaa taaccctttg
     4141 attgttttct aaaaggagac tccctcggca agatggcaga gggtacggag tcttcaggcc
     4201 cagtttctca ctttagccaa ttcgagggct ccttgtggtg ggatcagaac taatccagag
     4261 tgtgggaaag tgacagtcaa aaccccacct ggagcaaata aaaaaacata caaaacgtac
     4321 tggtgctttc ctgtctaagt cgccttttgt gtgttctttt atgaggcccc caccatccac
     4381 ccctctgtgc acaaggcggc tcccggcccc tggaatgtga tgtttttggt catctccaaa
     4441 ggctgcagtt ttatactggg aggctgatga caccttttat tataattatt cttatggttc
     4501 tggttataat tatgtttgtt ttaagatttt ctttaagaaa acaaaaaccc aacacccttc
     4561 cctttaggtt tcaaaccaag gtgcgggagc agggtgcttc tgagttagcg gtggctctgg
     4621 tgccctggct cccacccctc gggccaggtg ggccactggg catggtgaca gctgggcggc
     4681 cagggagtcc ccaaggtctg ccttctgggc ttgtcactct tctcttctac ttcccctcct
     4741 cttgagtcgg gtgtgtcagg gctggaggga ggaccggccg tctctctcct tgtgtgtgtg
     4801 attggagtgg tgtgtatttc ttttctagta cttggtctga tgatagctgt gctcttacct
     4861 cgagtcagca gcacctggag ccccaagtgc tcaacacttt gttctacttc ctacaaggca
     4921 ggcggcctgg cgtcagtagt aggaagggat ggcattgttg gctccagggt gagggcccga
     4981 cgggcaggtg cttgcctggg tagatggggt gtagatatgt ggcatataga gatatatatt
     5041 ttataatggg aggggggatg gcacctcctg ggactggtag agctgggagg tgtgtcggca
     5101 agcacatggc cccaagtcca cgtccctaga aagatggggc ctacagtgat aggcgctcta
     5161 taaatacata gatagggtca tgtttaagaa atattatggg gtggggagtg ggggaagcag
     5221 gcttagtgtg ctgagcctcg gtccccactg cagacctaaa ccccacacca atcagtcaat
     5281 ctcacacgca tacacaggag catatttctg tactgatgca taatgaatgc actcacacac
     5341 acacacacac gccagggggc tcccagcact cttctcccag ggttcaggac tttgtggagt
     5401 cagcccgtgc agctatttac ctctgttgcc ctgattgcag gtagactgtc aatcccagaa
     5461 gttgctaggt gctgaggcag gaaaaggagg aggtttcttt tagcaggtgc tctcctctag
     5521 caggtagtgt tctatctctt ttgcccctgg tgatgtcaaa agattcttct gttagcaacc
     5581 tgtgatactg acctgcaact ctaggcacca gtcaagtcag ccctcaactc cccctaccta
     5641 ttctccccaa ctgaaatcaa attgacatcc aggtctgtct tggccagggg gcttccattg
     5701 gggaaaaaaa atgggacagt gaacctcacc tattgaataa accaaattag taattagtga
     5761 caaaaccaaa ccaaggcttt ggaccccctg ctcattactc aggagtaaat tcacacagct
     5821 ggtggccggt ctcacccctt tttggggtgc ttggtcctgg accccaatct actgtctcct
     5881 cgacagattg gtgtttaaga tcatgcccag ttgcttcctg tcacctcttg acaggtggtg
     5941 actgggctgc tgtggggctg gagcagcgag gaagggagcc tctgccccct cagtggagtc
     6001 cctgtgctaa ctgtggtgct cacccagctc ctccttgctc ccgcggggct ctgtccttcg
     6061 ggcctggagt ccaccagcgc cactccagga gggctggggg aggaggggct gcctcaggca
     6121 gtccaaggtg ctccctcccc cgctggccaa tgctaaggtc ttcccggctc ccagggtcag
     6181 gacagagtct tctaaggctc aggctctccc aagccactgc ttagtgacag ttagcctttc
     6241 ccagcggccc aagctccacc ggaactgggc ctggtgctcc aaccaaaggg ttggtgttgg
     6301 gacttgggaa gagacttggt agccaagggc atggcaagcc tgggggattc ctgtgggccg
     6361 gcttgctcga ggagggagtg ctgggcacag aggagccctt atggctgaca gcttgagagc
     6421 acttgttcct ccagatctag ccacttgacc aggccctaga atggaccaga tgccccaggg
     6481 gtcatggccc cccccccccc ccccttgcag ggacgagagt taacaatcac cagcttttct
     6541 tccggcccag cctgtgtgct tggtctcaca gtagaattta ggagagtgtg gggctttggg
     6601 attgtgccaa cttggccaag ctgagatatg cttaatccac ctcaacgctg gggcctctgg
     6661 ggacaggtat tgggtcacct tccaaggggt gctgaacttc agcaggctgt agataaggca
     6721 gaggttagag gcagtggagt gggggaacca agaaggccta gggaaaattg ccaggcatat
     6781 tccctgtgga attttctttc cgaagagtag gcccatttcc cccttagaaa caagggttct
     6841 gggggctgat gacagcttcc cctgcaggtg ccaggtggcg catgatgcca aagagatccg
     6901 tggctcttgc ctgggggagg tagccaggtg ctcgtgctgg ggacacagtc cttggcctcc
     6961 tgcctaagca ggaagctagt ttccagcagg cggctggtac taaccccccg ccccacggga
     7021 gcgacaggct ggtgcaccgg cagactgggg ggccgcaggc gggcactagc aggggtctgc
     7081 agtccataga tgatgacatg aatctgttgc tgtccttttg tctctaggcc ccagagactt
     7141 cttgctattg aacatcctat tggaaagcaa actttccaag agagcacccg ggacctgtta
     7201 gcttttgaaa caggcctggg tgtgattgac tggtagagat gcctgtgtga tttttggcgc
     7261 attgcaggag tgtggattgg gacaggcacc ccagcaggaa ggaaggctga caccaaaggg
     7321 gtctcacagg gcccagctcc ccttgcttcc cgataattgg ccaccaggcc atggcgactc
     7381 tggggaagcc tagatgggaa cacccacctt ctccagcagt agaaggaata tttcagcaga
     7441 gccatctggg agatgttctt ataggaaaac tgcagtcatt catttcatag ttttgcttat
     7501 ataaatagag tacatttggg aggcagtttt gaattcctgt tgctactctg cttggtgcgg
     7561 agttgcagtg gccccaagtg tctttcagag ctgttgcttt taggatcccc ctcaagctgt
     7621 gggttagggc cagggcaggg agggggtgct gcctggcact gtgccctgaa gagggctgct
     7681 tgcttccttt ggtcgggagc acggcagagc caacattctg gtgggaaccg agtttctagc
     7741 caggcccctg ttttggtccc gggaggtgag taatctgagt ttgtcattgc gtattctcca
     7801 cgctgcctgc tctcagtgct ggagactaga gagcgaccct ggagttgtcc tctgggagtc
     7861 ctgcctgttt gccagggagc agctgaaacc tccccgctcc caatcccggg ggtggaaaag
     7921 gtgtagtgga ggaatggtct ggagctggga tggcccttct ggccatgccc tgctgagcca
     7981 ggattaagga aagtgtcagc cgcctcctta ccaggccctg tgctggggtc aggaggaccg
     8041 ggaggagagg gaaggggtga gaggatggac tctgctgttg tagtgtgagc tctggaatct
     8101 ttacgttttg gtaaaggcag gccagggtct ttgaggatcg agttggggaa ccttgcctct
     8161 gtgtttccga gacccagcta aggtgggcct ttggtgggtg acctgtctct gacccgaggc
     8221 agcagagttt tgggggaggg agaaacaagc aaggagcagg gaggacaagg acacaagatc
     8281 cctttgctgg tggtgactta ctcacttccc ccaccagagg gcctgggact cccgaaactc
     8341 ccgtaactgg aaagctccct ggctcagctc cactgggggg tgaggagggc ggctccctca
     8401 gccccagcct agcggcctcc aaccctgtgc ctgagaacat gagcagcggt ctaaagcatc
     8461 ccctagtccc atttcacttc acagaccgat ttggttgcac agccctagaa ttcaacagac
     8521 aacaaaaatg ctgaaattcc ttagaatacg cagagggagg aggtgactca ccaaggtgct
     8581 aaaacatttt attaaaaata tatattgtta aattaagtct gctgtctgga caatgactgt
     8641 ttgttttgtt ttcttgtagt ggagtttaaa agagactatt attttactct gatatattat
     8701 tattaaaaag gcattttaac tttgtacttg aaaactaaat gagcgatttc atgtgtttta
     8761 gctgaggcat tgaagtagcg ggtgcttact tatttgtggg aaatcatgta ttcatactga
     8821 agaataaact ccgtagctga tttggtaata acttttactg ttacagacga tttcgctttg
     8881 accccggtgg cagagcactt ttactccaca ttccacgcag ctaaatgcag gacttcccag
     8941 actcttccct ccccagccct ctttcctttc tcttaagttc ctcttttctc ctttattatt
     9001 attatttttt tttcctggtt ccagcatcct ttcaccactg tatttttttt tttttttttt
     9061 tttttttttt tttttttttt tttttttttt ttttagggtt gcttctctat ttattgacaa
     9121 taataatgta catttcacag tctggttcta ggacattcag ggaagggcga gtgggggccc
     9181 gaggggtccc tgccccaccc gcctggtgtc ctgttgtatc tctgtgtaag tgatgctcgt
     9241 gacatagctg tttatgaaat aattaaagag gtcaatgcgt acaacagatg tagaaagtct
     9301 gtcagttccg ccttcatcac attttttttt gtgcccagaa gaatataata aagctccttt
     9361 ctaatgtact tgtgctggag aacacttgaa taaatggact gtttttgtgc aaaaagaaaa
     9421 a
//
