LOCUS       NM_014280               1763 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member C8
            (DNAJC8), transcript variant 1, mRNA.
ACCESSION   NM_014280
VERSION     NM_014280.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Zhang,Z., Ju,M., Tang,Z., He,Z. and Hua,S.
  TITLE     DNAJC8: a prognostic marker and potential therapeutic target for
            hepatocellular carcinoma
  JOURNAL   Front Immunol 14, 1289548 (2024)
   PUBMED   38274804
  REMARK    GeneRIF: DNAJC8: a prognostic marker and potential therapeutic
            target for hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1763)
  AUTHORS   Wang,C.H., Shyu,R.Y., Wu,C.C., Chen,M.L., Lee,M.C., Lin,Y.Y.,
            Wang,L.K., Jiang,S.Y. and Tsai,F.M.
  TITLE     Tazarotene-Induced Gene 1 Interacts with DNAJC8 and Regulates
            Glycolysis in Cervical Cancer Cells
  JOURNAL   Mol Cells 41 (6), 562-574 (2018)
   PUBMED   29902837
  REMARK    GeneRIF: TIG1 interacted with DNAJC8 in the cytosol, and this
            interaction completely blocked DNAJC8-mediated PKM2 translocation
            and inhibited glucose uptake. Furthermore, increased glycose uptake
            was observed in cells in which TIG1 was silenced.
REFERENCE   4  (bases 1 to 1763)
  AUTHORS   Sokolina,K., Kittanakom,S., Snider,J., Kotlyar,M., Maurice,P.,
            Gandia,J., Benleulmi-Chaachoua,A., Tadagaki,K., Oishi,A., Wong,V.,
            Malty,R.H., Deineko,V., Aoki,H., Amin,S., Yao,Z., Morato,X.,
            Otasek,D., Kobayashi,H., Menendez,J., Auerbach,D., Angers,S.,
            Przulj,N., Bouvier,M., Babu,M., Ciruela,F., Jockers,R., Jurisica,I.
            and Stagljar,I.
  TITLE     Systematic protein-protein interaction mapping for clinically
            relevant human GPCRs
  JOURNAL   Mol Syst Biol 13 (3), 918 (2017)
   PUBMED   28298427
  REMARK    Erratum:[Mol Syst Biol. 2025 Jan 6. doi:
            10.1038/s44320-024-00080-3.. PMID: 39762653]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1763)
  AUTHORS   Ito,N., Kamiguchi,K., Nakanishi,K., Sokolovskya,A., Hirohashi,Y.,
            Tamura,Y., Murai,A., Yamamoto,E., Kanaseki,T., Tsukahara,T.,
            Kochin,V., Chiba,S., Shimohama,S., Sato,N. and Torigoe,T.
  TITLE     A novel nuclear DnaJ protein, DNAJC8, can suppress the formation of
            spinocerebellar ataxia 3 polyglutamine aggregation in a J-domain
            independent manner
  JOURNAL   Biochem Biophys Res Commun 474 (4), 626-633 (2016)
   PUBMED   27133716
REFERENCE   6  (bases 1 to 1763)
  AUTHORS   Behzadnia,N., Golas,M.M., Hartmuth,K., Sander,B., Kastner,B.,
            Deckert,J., Dube,P., Will,C.L., Urlaub,H., Stark,H. and Luhrmann,R.
  TITLE     Composition and three-dimensional EM structure of double
            affinity-purified, human prespliceosomal A complexes
  JOURNAL   EMBO J 26 (6), 1737-1748 (2007)
   PUBMED   17332742
REFERENCE   7  (bases 1 to 1763)
  AUTHORS   Andersen,J.S., Lam,Y.W., Leung,A.K., Ong,S.E., Lyon,C.E.,
            Lamond,A.I. and Mann,M.
  TITLE     Nucleolar proteome dynamics
  JOURNAL   Nature 433 (7021), 77-83 (2005)
   PUBMED   15635413
REFERENCE   8  (bases 1 to 1763)
  AUTHORS   Zhou,Z., Licklider,L.J., Gygi,S.P. and Reed,R.
  TITLE     Comprehensive proteomic analysis of the human spliceosome
  JOURNAL   Nature 419 (6903), 182-185 (2002)
   PUBMED   12226669
REFERENCE   9  (bases 1 to 1763)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
REFERENCE   10 (bases 1 to 1763)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB294321.1 and AL353354.36.
            
            On Nov 22, 2018 this sequence version replaced NM_014280.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.649834.1,
                                           SRR5189667.191551.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263697.6/ ENSP00000263697.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-708               DB294321.1         3-710
            709-1763            AL353354.36        5025-6079
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..1763
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="DnaJ heat shock protein family (Hsp40) member C8"
                     /db_xref="GeneID:22826"
                     /db_xref="HGNC:HGNC:15470"
     exon            1..109
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..793
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="splicing protein spf31; DnaJ (Hsp40) homolog,
                     subfamily C, member 8"
                     /codon_start=1
                     /product="dnaJ homolog subfamily C member 8"
                     /protein_id="NP_055095.2"
                     /db_xref="CCDS:CCDS41292.1"
                     /db_xref="GeneID:22826"
                     /db_xref="HGNC:HGNC:15470"
                     /translation="MAASGESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIE
                     RLTRPGSSYFNLNPFEVLQIDPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEA
                     VDKAYKLLLDQEQKKRALDVIQAGKEYVEHTVKERKKQLKKEGKPTIVEEDDPELFKQ
                     AVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDG
                     RVDSWRNFQANTKGKKEKKNRTFLRPPKVKMEQRE"
     exon            110..211
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            212..268
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            269..335
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            336..430
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            431..502
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            503..594
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            595..670
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     exon            671..1763
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1466..1471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="hexamer: AATAAA"
     polyA_site      1484
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="major polyA site"
     regulatory      1741..1746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="hexamer: ATTAAA"
     regulatory      1753..1758
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
                     /note="hexamer: AATATA"
     polyA_site      1758
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
     polyA_site      1763
                     /gene="DNAJC8"
                     /gene_synonym="HSPC331; SPF31"
ORIGIN      
        1 agaagggtca cgtggtggct gggccgggga aatggcggct tcaggagaga gcgggacttc
       61 aggcggcgga ggcagcaccg aggaagcatt tatgaccttc tacagtgagg tgaaacaaat
      121 agagaagaga gactcggttc taacttcgaa aaatcagatt gaaagactga cccgtcctgg
      181 ttcctcttac ttcaatttga acccatttga ggttcttcag atagatcctg aagttacaga
      241 tgaagaaata aaaaagaggt ttcggcagtt atccatcttg gtgcatcctg acaaaaatca
      301 agatgatgct gacagagcac aaaaggcttt tgaagctgtg gacaaagctt acaagttgct
      361 actggatcag gagcaaaaga agagggccct ggatgtaatt caggcaggaa aagaatacgt
      421 ggaacacact gtgaaagagc gaaaaaaaca attaaagaag gaaggaaaac ctacaattgt
      481 agaggaggat gatcctgagc tgttcaaaca agctgtatat aaacagacaa tgaaactctt
      541 tgcagagctg gaaattaaaa ggaaagagag agaagccaaa gagatgcatg aaaggaaacg
      601 acaaagggaa gaagagattg aagctcaaga aaaagccaaa cgggaaagag agtggcagaa
      661 aaactttgag gaaagtcgag atggtcgtgt ggacagctgg cgaaacttcc aagccaatac
      721 gaaggggaag aaagagaaga aaaatcggac cttcctgaga ccaccgaaag taaaaatgga
      781 gcaacgtgag tgaccgccca aggtcacagg cacagaacct ttcccctgct atctcccttc
      841 ctgcttcgaa ggactcattc tttcctccca cttccacccc aacatagagt agtatttgct
      901 ttttagtcca ttttgttttc aatacgattt aatatcgatc agagtaattc ttttgtacat
      961 tgaaatgagg ggcttggttt aaaaaaagac ctttccctct ccctgcccct agaacaacca
     1021 gtattagaag gtgccaccat tggtgctgcc ttctcttccc acagcctgta actcagtgtt
     1081 ttgtacttca ctgaattgtg atggttagaa acttcgtgga tagtttgtgg aaatcatcca
     1141 attaaacata ctgcttaaaa cagtgttgct gtgacttcag agacaagcct ggaaggggca
     1201 ccttaggaag ccccttcgct tcagttgctc gcttctgggt gtgctccctt cgaaggccca
     1261 gataagacag ggaacacttg tgagcacaca gagcagcatc tgatgccctg tggtgtttgg
     1321 catgtgcccc ctgtctactg accaatcagt gtggcatgag gcccacgcca cccaaacctt
     1381 tcactttcca aagagctagc cgtcctccac ccagtaccat gtcctagcct gtctgcattt
     1441 gttagtggta atattcttta tgtataataa atttttatac ccaagccatt gatgtacttt
     1501 tccttgtact ctcccttgtg ggtcccttgt ctggcttggc tgaaccccaa aatgctttgg
     1561 ggttggacag acctggctga accttagttt cttcatctat gaaatgggaa tatgaattac
     1621 tgcagcagct tttagggcag atttgccatg gcatatacaa ggtaactacc atagtgctcc
     1681 ttgggtattg ccaatatcct attatttctg tgtaaaatga agatactgat tgttttgagg
     1741 attaaatgaa gtaatatatg aaa
//
