LOCUS       NM_001256864            5962 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member C6
            (DNAJC6), transcript variant 1, mRNA.
ACCESSION   NM_001256864
VERSION     NM_001256864.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Chiu,C.C., Chen,Y.L., Weng,Y.H., Liu,S.Y., Li,H.L., Yeh,T.H. and
            Wang,H.L.
  TITLE     Downregulation of Protease Cathepsin D and Upregulation of
            Pathologic alpha-Synuclein Mediate Paucity of DNAJC6-Induced
            Degeneration of Dopaminergic Neurons
  JOURNAL   Int J Mol Sci 25 (12), 6711 (2024)
   PUBMED   38928416
  REMARK    GeneRIF: Downregulation of Protease Cathepsin D and Upregulation of
            Pathologic alpha-Synuclein Mediate Paucity of DNAJC6-Induced
            Degeneration of Dopaminergic Neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Benussi,L., Longobardi,A., Kocoglu,C., Carrara,M., Bellini,S.,
            Ferrari,C., Nicsanu,R., Saraceno,C., Bonvicini,C., Fostinelli,S.,
            Zanardini,R., Catania,M., Moisse,M., Van Damme,P., Di Fede,G.,
            Binetti,G., Van Broeckhoven,C., van der Zee,J. and Ghidoni,R.
  TITLE     Investigating the Endo-Lysosomal System in Major Neurocognitive
            Disorders Due to Alzheimer's Disease, Frontotemporal Lobar
            Degeneration and Lewy Body Disease: Evidence for SORL1 as a
            Cross-Disease Gene
  JOURNAL   Int J Mol Sci 22 (24), 13633 (2021)
   PUBMED   34948429
  REMARK    GeneRIF: Investigating the Endo-Lysosomal System in Major
            Neurocognitive Disorders Due to Alzheimer's Disease, Frontotemporal
            Lobar Degeneration and Lewy Body Disease: Evidence for SORL1 as a
            Cross-Disease Gene.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5962)
  AUTHORS   Wulansari,N., Darsono,W.H.W., Woo,H.J., Chang,M.Y., Kim,J.,
            Bae,E.J., Sun,W., Lee,J.H., Cho,I.J., Shin,H., Lee,S.J. and
            Lee,S.H.
  TITLE     Neurodevelopmental defects and neurodegenerative phenotypes in
            human brain organoids carrying Parkinson's disease-linked DNAJC6
            mutations
  JOURNAL   Sci Adv 7 (8) (2021)
   PUBMED   33597231
  REMARK    GeneRIF: Neurodevelopmental defects and neurodegenerative
            phenotypes in human brain organoids carrying Parkinson's
            disease-linked DNAJC6 mutations.
            Erratum:[Sci Adv. 2023 Nov 3;9(44):eadl1498. doi:
            10.1126/sciadv.adl1498.. PMID: 37922364]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5962)
  AUTHORS   Ng,J., Cortes-Saladelafont,E., Abela,L., Termsarasab,P., Mankad,K.,
            Sudhakar,S., Gorman,K.M., Heales,S.J.R., Pope,S., Biassoni,L.,
            Csanyi,B., Cain,J., Rakshi,K., Coutts,H., Jayawant,S.,
            Jefferson,R., Hughes,D., Garcia-Cazorla,A., Grozeva,D.,
            Raymond,F.L., Perez-Duenas,B., De Goede,C., Pearson,T.S., Meyer,E.
            and Kurian,M.A.
  TITLE     DNAJC6 Mutations Disrupt Dopamine Homeostasis in Juvenile
            Parkinsonism-Dystonia
  JOURNAL   Mov Disord 35 (8), 1357-1368 (2020)
   PUBMED   32472658
  REMARK    GeneRIF: DNAJC6 Mutations Disrupt Dopamine Homeostasis in Juvenile
            Parkinsonism-Dystonia.
REFERENCE   5  (bases 1 to 5962)
  AUTHORS   Shen,T., Zhao,S., Jiang,Y., Pu,J., Lai,H.Y., Yan,Y., Tian,J.,
            Chen,Y. and Zhang,B.
  TITLE     Haplotype Analysis on the Relationship of the DNAJC6 Gene with
            Early-Onset Parkinson's Disease Risk in a Chinese Population
  JOURNAL   J Parkinsons Dis 9 (1), 109-120 (2019)
   PUBMED   30373961
  REMARK    GeneRIF: Early onset Parkinson is associated with several SNPs and
            haplotypes of DNAJC6 gene.
REFERENCE   6  (bases 1 to 5962)
  AUTHORS   Scheele,U., Kalthoff,C. and Ungewickell,E.
  TITLE     Multiple interactions of auxilin 1 with clathrin and the AP-2
            adaptor complex
  JOURNAL   J Biol Chem 276 (39), 36131-36138 (2001)
   PUBMED   11470803
REFERENCE   7  (bases 1 to 5962)
  AUTHORS   Kirchhausen,T.
  TITLE     Three ways to make a vesicle
  JOURNAL   Nat Rev Mol Cell Biol 1 (3), 187-198 (2000)
   PUBMED   11252894
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol 2001 Mar;2(3):216]
REFERENCE   8  (bases 1 to 5962)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
REFERENCE   9  (bases 1 to 5962)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   10 (bases 1 to 5962)
  AUTHORS   Ungewickell,E., Ungewickell,H., Holstein,S.E., Lindner,R.,
            Prasad,K., Barouch,W., Martin,B., Greene,L.E. and Eisenberg,E.
  TITLE     Role of auxilin in uncoating clathrin-coated vesicles
  JOURNAL   Nature 378 (6557), 632-635 (1995)
   PUBMED   8524399
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR002818.1, BX647203.1,
            BC109280.1 and AC119800.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001256864.1.
            
            Summary: DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40
            family of proteins, which regulate molecular chaperone activity by
            stimulating ATPase activity. DNAJ proteins may have up to 3
            distinct domains: a conserved 70-amino acid J domain, usually at
            the N terminus, a glycine/phenylalanine (G/F)-rich region, and a
            cysteine-rich domain containing 4 motifs resembling a zinc finger
            domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by
            OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647203.1, SRR1660809.45874.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371069.5/ ENSP00000360108.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 DR002818.1         14-15
            3-56                BX647203.1         1-54
            57-3838             BC109280.1         1-3782
            3839-5962           AC119800.2         11943-14066
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..5962
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /note="DnaJ heat shock protein family (Hsp40) member C6"
                     /db_xref="GeneID:9829"
                     /db_xref="HGNC:HGNC:15469"
                     /db_xref="MIM:608375"
     exon            1..396
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..3116
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /EC_number="3.1.3.48"
                     /note="isoform 1 is encoded by transcript variant 1;
                     auxilin; DnaJ (Hsp40) homolog, subfamily B, member 6;
                     putative tyrosine-protein phosphatase auxilin; DnaJ
                     (Hsp40) homolog, subfamily C, member 6"
                     /codon_start=1
                     /product="auxilin isoform 1"
                     /protein_id="NP_001243793.1"
                     /db_xref="CCDS:CCDS58004.1"
                     /db_xref="GeneID:9829"
                     /db_xref="HGNC:HGNC:15469"
                     /db_xref="MIM:608375"
                     /translation="MSLLGSYRKKTSNDGYESLQLVDSNGDLSAGSGGVGGKQRVNAG
                     AAARSPARQPPDRASTMDSSGASSPDMEPSYGGGLFDMVKGGAGRLFSNLKDNLKDTL
                     KDTSSRVIQSVTSYTKGDLDFTYVTSRIIVMSFPLDNVDIGFRNQVDDIRSFLDSRHL
                     DHYTVYNLSPKSYRTAKFHSRVSECSWPIRQAPSLHNLFAVCRNMYNWLLQNPKNVCV
                     VHCLDGRAASSILVGAMFIFCNLYSTPGPAIRLLYAKRPGIGLSPSHRRYLGYMCDLL
                     ADKPYRPHFKPLTIKSITVSPIPFFNKQRNGCRPYCDVLIGETKIYSTCTDFERMKEY
                     RVQDGKIFIPLNITVQGDVVVSMYHLRSTIGSRLQAKVTNTQIFQLQFHTGFIPLDTT
                     VLKFTKPELDACDVPEKYPQLFQVTLDVELQPHDKVIDLTPPWEHYCTKDVNPSILFS
                     SHQEHQDTLALGGQAPIDIPPDNPRHYGQSGFFASLCWQDQKSEKSFCEEDHAALVNQ
                     ESEQSDDELLTLSSPHGNANGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSN
                     SFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTPRRSATST
                     SASPTLRVGEGATFDPFGAPSKPSGQDLLGSFLNTSSASSDPFLQPTRSPSPTVHASS
                     TPAVNIQPDVSGGWDWHAKPGGFGMGSKSAATSPTGSSHGTPTHQSKPQTLDPFADLG
                     TLGSSSFASKPTTPTGLGGGFPPLSSPQKASPQPMGGGWQQGGAYNWQQPQPKPQPSM
                     PHSSPQNRPNYNVSFSAMPGGQNERGKGSSNLEGKQKAADFEDLLSGQGFNAHKDKKG
                     PRTIAEMRKEEMAKEMDPEKLKILEWIEGKERNIRALLSTMHTVLWAGETKWKPVGMA
                     DLVTPEQVKKVYRKAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGQKPLY"
     exon            397..547
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            548..597
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            598..746
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            747..869
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1003
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1198
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1316
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1396
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1590
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1671
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..2106
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2241
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2310
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2430
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2694
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2837
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..3014
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..5962
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5349..5354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /note="hexamer: ATTAAA"
     polyA_site      5367
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /note="major polyA site"
     regulatory      5403..5408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /note="hexamer: AATACA"
     polyA_site      5427
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
     regulatory      5931..5936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
                     /note="hexamer: AATAAA"
     polyA_site      5962
                     /gene="DNAJC6"
                     /gene_synonym="DJC6; PARK19"
ORIGIN      
        1 gagccgagct cagcccaggg cggcggcttc gcctcgcccg gcgaagcttc tctccggtgg
       61 ccgctccttc ttttccctcc tctttgcgtc atgtcgggca cttaattttt tttttttttt
      121 aattccttct tcagcccttt ccaccttcca tctccctttt cgcttcccag gttgattatt
      181 ttctcttttc tccgggcttg cccatgagcc tcctcgggag ctaccggaaa aagaccagca
      241 acgatggtta tgaatctttg cagctggtgg acagtaacgg ggacttaagt gcgggaagcg
      301 gcggggttgg cggcaagcag agagtgaacg ccggggcagc ggcgcggagt cccgcccgac
      361 agcctccgga ccgcgccagc accatggaca gctcaggtgc ctcatctcca gacatggagc
      421 ccagctatgg gggaggtctc tttgacatgg taaaaggagg tgcagggagg ctctttagta
      481 acctaaagga caacttgaaa gacaccctca aagacacatc ttctagagtg atacaatctg
      541 tgaccagcta cacaaaggga gatttagact tcacttatgt tacctccaga attattgtga
      601 tgtcctttcc tctggacaat gttgacatag gattcaggaa tcaggttgat gacattcgaa
      661 gctttttgga ttccagacat cttgaccact acacagtata caatctgtca cctaagtctt
      721 atcgaactgc caagtttcac agccgggtct cagaatgcag ttggcccatt aggcaggctc
      781 ccagtctgca caaccttttt gctgtgtgtc ggaatatgta taactggcta ctgcagaatc
      841 ccaaaaatgt ctgtgttgtc cactgcttgg atggacgggc ggcatcatca attctggttg
      901 gtgctatgtt cattttctgt aatctctact ctactcctgg cccagccatt cgattgctat
      961 atgcaaagcg accaggaatt ggactttcac catcccatag gagatacctg ggctatatgt
     1021 gtgacctact ggcagacaag ccctaccgcc ctcacttcaa gcctctcaca attaagtcga
     1081 tcactgtcag tccaataccc tttttcaaca aacagaggaa tggatgtcgc ccttactgtg
     1141 atgtactcat tggagaaacc aaaatatatt cgacttgcac agattttgaa cgaatgaaag
     1201 aatatcgtgt ccaagatgga aaaatcttca ttcccttgaa catcactgtg caaggagacg
     1261 tggttgtttc catgtatcac ttgaggtcaa ccattgggag ccggctacag gctaaggtga
     1321 ccaacacaca gatattccag cttcagtttc acactggatt cataccactg gacacaacag
     1381 ttttaaagtt caccaagcct gagttagatg catgtgatgt accagaaaaa tatcctcagc
     1441 tatttcaggt gacactggat gtagaactac agccccatga caaagtaata gacttaactc
     1501 caccatggga acattactgc acaaaagatg tcaatcccag catcctcttc tcttctcacc
     1561 aggaacatca agatacgctg gccttaggag gacaggctcc aatagatatc cctccagaca
     1621 accccaggca ttacggacaa agtggtttct ttgcctctct ctgttggcaa gatcagaaat
     1681 cggagaagtc attctgtgag gaggaccacg ctgccctagt gaatcaggaa agtgagcaat
     1741 cagatgatga acttctgaca ctttccagtc cgcatggcaa tgccaatggt gacaagcctc
     1801 atggagtcaa gaagcccagc aaaaagcagc aggagccagc agcccctcca ccccctgagg
     1861 atgtggacct tttgggcctg gaagggtctg caatgagtaa cagcttctct ccgccagcgg
     1921 ctcctcccac caattctgaa ctactgagtg acctgtttgg gggtggaggt gcagctggtc
     1981 ccacccaggc tggacagtca ggagtggaag atgtgtttca tcctagtgga cctgcgtcta
     2041 cccagtcaac accacgccgc tctgccacct ccacctctgc gtctccaacc ctaagagtgg
     2101 gagaaggtgc cacctttgac ccatttggag caccttctaa accatcaggt caggatttgc
     2161 tgggttcttt tctgaacaca tccagtgctt ccagtgaccc ctttctccag ccaacaagaa
     2221 gtccttcgcc cacagtacat gcttctagta cgcctgctgt gaacattcag ccagatgttt
     2281 ctggaggttg ggactggcat gctaaaccag gaggctttgg aatgggaagc aagtcagctg
     2341 ccaccagccc aaccggatcc tcgcatggta ctcccaccca tcaaagcaaa ccccagactc
     2401 tggatccttt tgccgacctt gggacactag gtagttcttc ctttgccagc aaacccacca
     2461 caccaactgg attgggtgga ggattcccgc ctctcagctc gccacagaag gcgtctcccc
     2521 agcctatggg tggcgggtgg cagcagggag gtgcctacaa ctggcagcag ccacagccta
     2581 agcctcagcc cagcatgccc cactcctctc cccagaaccg acccaactac aacgtgagct
     2641 tctcagccat gcctgggggc cagaacgaac gtgggaaagg atcaagtaat ttggaaggga
     2701 aacaaaaagc agctgatttt gaagacctac tctctggtca aggtttcaat gctcacaaag
     2761 acaaaaaggg gcctcggaca atagctgaga tgagaaagga ggaaatggcc aaggaaatgg
     2821 atcctgagaa attaaagatt ctggaatgga ttgaaggcaa agaaagaaat atcagagccc
     2881 ttctttccac gatgcatacc gtactatggg ctggggagac caagtggaaa ccagttggca
     2941 tggcagacct ggtaacacca gagcaggtga agaaggtgta caggaaggct gtcctggtgg
     3001 tgcacccaga taaagctact gggcaaccct atgaacaata cgcaaagatg attttcatgg
     3061 agctcaatga tgcctggtct gaatttgaaa accaaggcca aaagccctta tattaattta
     3121 tgagcttttc catctctgct gcagacctgt gctaatgctt agtgtgtgtc acaattctga
     3181 ggttttcgca gatgaaccaa aaactccagt aacatgtttt cagtactaaa ccgttaagtt
     3241 actcatgaat taatttctca ttgataagga atgtggatgt ttggtttctc caaagttccc
     3301 accataaaag atccaaagca tgagaggaac ttctagtcag atgaccttgc agaaccaccg
     3361 cattccaccc tgccctttgg ggagcctact cagcattcta cctggggaaa tggaaaacag
     3421 aggccaccac ccatgaaggc ataacaccca tcacattgtc tgagaatagg attaatgagc
     3481 aaaagttatg ataatagagt tatatgatgg tttaatggtt tctagatttt tcttggaaag
     3541 atataatttg agcagtggct ttatgcagac tccaagaaca aaaccaccag gtattttttt
     3601 ttaagtaaaa atttactaag gcctttagca taatactgta aatgtctttt ggtcaagaga
     3661 actaacaaat ataaaggaac ttgaaatgtg gggagtgata atagtccagt gattaaacta
     3721 cagtccagtg attaccacca actttctgac taagctaaaa atgaatgaaa atggtggcta
     3781 aagaaaatag ggactctgaa gatgtcgaaa tctttattcc tggttaggaa ggtgggccgc
     3841 tacccttctt tccctttaga aaactgtgat taattaaata aaaagtctta ctactataac
     3901 aactctaaac ctggtttaaa tgaaacatct aattttgctg taaggcttaa tgctcttctt
     3961 ttgtttagcc aagaaaacat tcttagttgg aaacaggtgg taaagtagtt tggcctgttc
     4021 acaacttatc aggaccaata tctagtgtgg actcagtgtt ctctaggttt gtgagcagtt
     4081 gtggtaagag aatggaaggc aacactgaat aggagttaag ggaatggcac gatcacagtg
     4141 agagagctgc actcacctgc agacccagtc agatggcttg tcagtatctg ctctgaggaa
     4201 ccaggtccca cctccccaca aggcaaatct agacccatta aattattaca gtgggctttt
     4261 ttgttttaaa agtggttggg gatccacagg aacgacattc atacagggac atttgtgaaa
     4321 gcaaagcaag aatgaatgct ttcccgatct cagactggct ggattcagat cagtgtgttg
     4381 gctggttctc attttaaggg gtaagcagtt tgctattctg tgactttagt agatctcttt
     4441 tgtgtgcaca tatacaatgt gcattataca tatatattaa atatatccac aaagcagatg
     4501 ggcaaaaaga ttttcatgct aatcttcaaa ggttcatgct caatattgtg aaaagctttt
     4561 aaaagttgtt ttgaaaagac aatcattttt tgtttttgtt ttaactttct tgtggcaagc
     4621 tataccggaa aagcggcatg tcaaaaatac tttaggttct acatagatct ctttctctct
     4681 tttgttttgt ttgtttgttt gttaaagttc attatttatt ttacctttta ctgacaatgt
     4741 gaaactcgaa gaaaatgttc tctttttaaa tatacagtca taaactgttc aaggtaacca
     4801 taacaaacta ggtgttattt attaacgtat tataaaataa tgtttgatcc agtatgtgct
     4861 tgtcttattt aaaattggaa tgtgagacat gttgctgtga cctgtttttc tttctcattc
     4921 acatttgtag atattgtgtg aactacagta tataatgata acaattaaaa ggatattctg
     4981 tggatgtcac gtattttgaa atgatagaac tacattagct ttgtatcatg tttggataat
     5041 tcatcaatgt tcacagttta aaacatcatt aaacattatg taattacaat gagaaagaat
     5101 cttacttaaa tttggagatt ttcccccaca tctcttttcc ggatacatta taattctgga
     5161 cccctattta tctcaaaact cttaatatat gcagaccaac aggtctttgc attcctttta
     5221 aataactggt tgtgacaaag cttgttgttg atcagattca ctgtttcatc atatttattg
     5281 taatatattt tttgttttgt aaatatgtta caacaaaatg tgattggtct aaaatatttg
     5341 taatgtatat taaaaggttc aaaaatattt gtataaatct aagttatttg ggtacttgta
     5401 ggaatacaat gaggagcttg aatgccacct tctgacatga tttactttta aggaaagtta
     5461 tgaggagaga atacaaaaag catgcaccaa aatgtaatct gacaggattt ctggatttat
     5521 acgtaatcac tcctgcccca acacacacac acacacacac acacacacac acacacatgc
     5581 acgcacacat gattttgatt atgctacatg atatatgttt caaaaagaat tagcatagaa
     5641 atcctggtct cctagccaaa aaaatcaaag gattttcaaa aaaacgaatc tgtatgttga
     5701 ggcaaaagga ttgaacctgg aagtctggga ctttatcata gaaacaaagt ctcagatatt
     5761 ttagttcttt ggaaacaaat gctgtaattc aaaagcattt gacctgtcac tgtactatct
     5821 acatgtggaa gaatgttcaa gttgaatcct aatgccgtga atgaaacaca gtctgtgtag
     5881 ggaatgagca aaaaagttga attccaattg ctttttggcc ttttggctaa aataaatgta
     5941 gcatctaatt ttatcagttt aa
//
