LOCUS       NM_181706               2970 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member C24
            (DNAJC24), mRNA.
ACCESSION   NM_181706
VERSION     NM_181706.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Liu,D., Zuo,R., Liu,W., He,Y., Wang,Y., Yue,P., Gong,W., Cui,J.,
            Zhu,F., Luo,Y., Qi,L., Guo,Y., Chen,L., Li,G., Liu,Z., Chen,P. and
            Guo,H.
  TITLE     DNAJC24 acts directly with PCNA and promotes malignant progression
            of LUAD by activating phosphorylation of AKT
  JOURNAL   FASEB J 38 (9), e23630 (2024)
   PUBMED   38713100
  REMARK    GeneRIF: DNAJC24 acts directly with PCNA and promotes malignant
            progression of LUAD by activating phosphorylation of AKT.
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Hoffmann,T.J., Tang,H., Thornton,T.A., Caan,B., Haan,M.,
            Millen,A.E., Thomas,F. and Risch,N.
  TITLE     Genome-wide association and admixture analysis of glaucoma in the
            Women's Health Initiative
  JOURNAL   Hum Mol Genet 23 (24), 6634-6643 (2014)
   PUBMED   25027321
REFERENCE   3  (bases 1 to 2970)
  AUTHORS   Wei,H., Xiang,L., Wayne,A.S., Chertov,O., FitzGerald,D.J.,
            Bera,T.K. and Pastan,I.
  TITLE     Immunotoxin resistance via reversible methylation of the DPH4
            promoter is a unique survival strategy
  JOURNAL   Proc Natl Acad Sci U S A 109 (18), 6898-6903 (2012)
   PUBMED   22509046
  REMARK    GeneRIF: immunotoxin resistance is associated with reversible CpG
            island methylation and silencing of DPH4 gene transcription
REFERENCE   4  (bases 1 to 2970)
  AUTHORS   Thakur,A., Chitoor,B., Goswami,A.V., Pareek,G., Atreya,H.S. and
            D'Silva,P.
  TITLE     Structure and mechanistic insights into novel iron-mediated
            moonlighting functions of human J-protein cochaperone, Dph4
  JOURNAL   J Biol Chem 287 (16), 13194-13205 (2012)
   PUBMED   22367199
  REMARK    GeneRIF: We report here the solution structure and mechanism of
            novel iron-mediated functional roles of human Dph4, a type III
            J-protein playing a vital role in diphthamide biosynthesis and
            normal development.
REFERENCE   5  (bases 1 to 2970)
  AUTHORS   Liu,S., Milne,G.T., Kuremsky,J.G., Fink,G.R. and Leppla,S.H.
  TITLE     Identification of the proteins required for biosynthesis of
            diphthamide, the target of bacterial ADP-ribosylating toxins on
            translation elongation factor 2
  JOURNAL   Mol Cell Biol 24 (21), 9487-9497 (2004)
   PUBMED   15485916
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833128.1 and AC108456.7.
            
            On Nov 22, 2018 this sequence version replaced NM_181706.4.
            
            Summary: Diphthamide is a unique posttranslationally modified
            histidine found only in translation elongation factor-2 (EEF2; MIM
            130610). This modification is conserved from archaebacteria to
            humans and serves as the target for ADP-ribosylation and
            inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas
            exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of
            diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied
            by OMIM, Mar 2008].
            
            CCDS Note: This CCDS representation uses the 5'-most in-frame start
            codon, which is conserved in other species. An alternative
            downstream start codon, which has a stronger Kozak signal, also
            exists. It is possible that leaky scanning by ribosomes would allow
            the downstream start codon to be used, at least some of the time.
            The use of the downstream start codon would result in a protein
            that is 1 aa shorter at the N-terminus. There is no experimental
            evidence showing which start codon is preferentially used in vivo.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA659255.1, SRR3476690.655970.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000465995.6/ ENSP00000417548.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-622               AL833128.1         21-642
            623-2970            AC108456.7         42515-44862
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p13"
     gene            1..2970
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /note="DnaJ heat shock protein family (Hsp40) member C24"
                     /db_xref="GeneID:120526"
                     /db_xref="HGNC:HGNC:26979"
                     /db_xref="MIM:611072"
     exon            1..53
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            54..197
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..536
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /note="zinc finger, CSL domain containing 3;
                     1700030A21Rik; DPH4 homolog (JJJ3, S. cerevisiae); zinc
                     finger, CSL-type containing 3; diphthamide biosynthesis
                     protein 4; CSL-type zinc finger-containing protein 3;
                     DPH4, JJJ3 homolog; DnaJ (Hsp40) homolog, subfamily C,
                     member 24"
                     /codon_start=1
                     /product="dnaJ homolog subfamily C member 24"
                     /protein_id="NP_859057.4"
                     /db_xref="CCDS:CCDS7873.2"
                     /db_xref="GeneID:120526"
                     /db_xref="HGNC:HGNC:26979"
                     /db_xref="MIM:611072"
                     /translation="MMAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQS
                     TDVPAGTVEECVQKFIEIDQAWKILGNEETKREYDLQRCEDDLRNVGPVDAQVYLEEM
                     SWNEGDHSFYLSCRCGGKYSVSKDEAEEVSLISCDTCSLIIELLHYN"
     exon            198..336
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            337..405
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            406..2970
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2951..2956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /note="hexamer: AATAAA"
     polyA_site      2970
                     /gene="DNAJC24"
                     /gene_synonym="DPH4; JJJ3; ZCSL3"
                     /note="major polyA site"
ORIGIN      
        1 agttctggcc gacagcaggc gaggagtggg tagcagcgcc tatgtgaagt tagctaatct
       61 gagaaggccc acttctggtt ccatggatga tggcggttga gcagatgcca aaaaaggatt
      121 ggtacagcat cctgggagca gacccatctg caaatatatc agacctaaaa caaaaatatc
      181 aaaaactcat attaatgtat catccagata aacaaagtac agatgtacca gcaggaacag
      241 tggaggaatg tgtacagaag ttcatcgaaa ttgatcaagc atggaaaatt ctaggaaatg
      301 aagagacaaa aagagagtat gacctgcagc ggtgtgaaga tgatctaaga aatgtaggac
      361 cagtagatgc tcaagtatat cttgaagaaa tgtcttggaa tgaaggtgat cactcttttt
      421 atctgagttg cagatgtggt ggaaaataca gtgtttccaa ggatgaagcg gaagaagtta
      481 gcctgatttc ttgtgataca tgttcactaa ttatagaact ccttcattat aactaaaatt
      541 gttcacaact tgaaatgctt taactgtggt attgagacat gatgagaagc cgttgagctt
      601 tgtccattca aggaaatgga ttatttgtca gcccgattat ttgcaaagaa aatatacaat
      661 tatcaagcag agaccacctc agattaatag agaatgaata taattattta tttgtattta
      721 taatttatat ttacaagttg tcacaaaaat agatttgatt atatttggtg aattcccatc
      781 tgagaacttt attatgtcat ttttttccat gacacaaaat aagaattttc ttaatatgag
      841 catgtctcct cttttcagtg ggtaacagta tcatcatatt cctttatagt tactaagttt
      901 ctggtctaac cgataacgtt tctagttgct aaagttatgg ggctgttgta cacctagaat
      961 ctttgtgaat aatgtgaggc cagttcttcc ataaggaagg ctggttatgg atattcataa
     1021 ggttatttca aagttaataa agacaaagtg gcaactgtag aaagtgttgc ctccaatctt
     1081 ggtccgtatt tccaaagctt gtaaacactg ccatcttcaa atttaaatgt aatttaggaa
     1141 atggaagctt ttaattttaa tttctttttt ctaagacaga gtctcactct gtcgcgccag
     1201 ctggagtgca gtgccatgat ctcaggtcgc tgtaacctcc gtctcctggg ttcaagtgat
     1261 tctccagcct cagcctcccg aatagctggg attacacgtg cccaccacca tgcccagcta
     1321 atttttgtat ttttagtaga gatggggttc caccatgttg gccaggctgg tctcaaactc
     1381 ctgacctcag gtgatccacc tgcctcgacc tcccaaagtg ctaggattac aggtttgagc
     1441 caccgtgcct ggcctaagtt ttgttaatag agatgggatt tcatcatgtt ggctaggctg
     1501 gtcttgaact cctggcctca aatgatccgc ccacctcaac ctcccaaagt gctgggattg
     1561 caggcgtgag ccaccatgcc tggccagaag cttttttaaa aataatgact tggaagttgg
     1621 tgcattattt ccccttttat tttggagtgt tgatataata ttggggccag gcatggtggc
     1681 tcacatctgt aatcccagca ctttgggaga ctaaggcagg cggatcacct ttggtcagga
     1741 gttcaagacc accctggcca acatggtgaa accccatatc tactaaaaat aattttttta
     1801 aaaattagcc agtcacgttg gtgtctgcct gtggtcccag ctactcggga ggctgaggca
     1861 caagaatcac ttgaacccag gaggcagagt gagccgaggt tgtgccactg cactgcagcc
     1921 tgggctacag agagcaagac tctgtctcaa aaaaaataaa ataaaataaa taaaaaaaga
     1981 tatagtatta atgcctgtat ttatgaagac agggccattg cactgctgct atgctgaaat
     2041 gaacttttat aaagtattat tgactatagc caactacaga tggcatatgc cttaaagatg
     2101 catttttcta tgtatttaat attttgtgta cagtaaaagt cagaactcat ttaaagaact
     2161 ttaagggatc ctttagaaac atttttactt aatttcaaat gagtgggctg agcatgtaga
     2221 ctagcagctt tttatcaaac ctggcattgt caggttgaac ttataaatac gaatagttac
     2281 tactctgact tgtaaatgta gctgctaaag tatgaggtat atttaagaag aatagattag
     2341 tttcattata cctataagaa caagaattca aaagtgaggt agtcatccag gttccccctc
     2401 ccacaatatt tttgtatcat aaaatttaga tgactttttt gggttacatt tttatccata
     2461 tattttgaac taacagaact tggcagaatg tgtgttgaat attctttaca tttaacaatt
     2521 aaaaacaact aaaactgaat agcaaatagt ttattggtaa gtacacggtt tcaacgggag
     2581 taataaattc acatgaaaag gagacaataa tcaagtcaaa agaataaatg cttactaatc
     2641 atcagaaaat ctgtggccat tagggctggc acgtaaaaat ccaaaatcac tcagaggcca
     2701 aatctgtaaa atcaaaatga ggttgaagac aattagtgaa agtaatgtta tagtatcatc
     2761 atattccctt ttgctatctg tcccttttct ctatgccaga taaacacttt ctccttactc
     2821 atcaatcaag aataaaattt tctacatcaa agtaagactt gagaaaataa acttatgtgt
     2881 atatatgttt aaactccaaa gtttgggaca ttcaaacaat gtacatgaaa ttatatgtgt
     2941 gtggtgtgtg aataaatatg tatgaatata
//
