LOCUS       NM_001012339            6107 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member C21
            (DNAJC21), transcript variant 2, mRNA.
ACCESSION   NM_001012339
VERSION     NM_001012339.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Morini,J., Nacci,L., Babini,G., Cesaro,S., Valli,R., Ottolenghi,A.,
            Nicolis,E., Pintani,E., Maserati,E., Cipolli,M., Danesino,C.,
            Scotti,C. and Minelli,A.
  TITLE     Whole exome sequencing discloses heterozygous variants in the
            DNAJC21 and EFL1 genes but not in SRP54 in 6 out of 16 patients
            with Shwachman-Diamond Syndrome carrying biallelic SBDS mutations
  JOURNAL   Br J Haematol 185 (3), 627-630 (2019)
   PUBMED   30198570
  REMARK    GeneRIF: Whole exome sequencing discloses heterozygous variants in
            the DNAJC21 and EFL1 genes but not in SRP54 in 6 out of 16 patients
            with Shwachman-Diamond Syndrome carrying biallelic SBDS mutations.
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   D'Amours,G., Lopes,F., Gauthier,J., Saillour,V., Nassif,C.,
            Wynn,R., Alos,N., Leblanc,T., Capri,Y., Nizard,S., Lemyre,E.,
            Michaud,J.L., Pelletier,V.A., Pastore,Y.D. and Soucy,J.F.
  TITLE     Refining the phenotype associated with biallelic DNAJC21 mutations
  JOURNAL   Clin Genet 94 (2), 252-258 (2018)
   PUBMED   29700810
  REMARK    GeneRIF: We suggest that DNAJC21-related diseases constitute a
            distinct Inherited bone marrow failure syndromes (IBMFS) , with
            features overlapping Shwachman-Diamond syndrome and Dyskeratosis
            congenita, and additional characteristics that are specific to
            DNAJC21 mutations
REFERENCE   3  (bases 1 to 6107)
  AUTHORS   Warren,A.J.
  TITLE     Molecular basis of the human ribosomopathy Shwachman-Diamond
            syndrome
  JOURNAL   Adv Biol Regul 67, 109-127 (2018)
   PUBMED   28942353
  REMARK    Review article
REFERENCE   4  (bases 1 to 6107)
  AUTHORS   Dhanraj,S., Matveev,A., Li,H., Lauhasurayotin,S., Jardine,L.,
            Cada,M., Zlateska,B., Tailor,C.S., Zhou,J., Mendoza-Londono,R.,
            Vincent,A., Durie,P.R., Scherer,S.W., Rommens,J.M., Heon,E. and
            Dror,Y.
  TITLE     Biallelic mutations in DNAJC21 cause Shwachman-Diamond syndrome
  JOURNAL   Blood 129 (11), 1557-1562 (2017)
   PUBMED   28062395
  REMARK    GeneRIF: Biallelic mutations in DNAJC21 cause Shwachman-Diamond
            syndrome.
REFERENCE   5  (bases 1 to 6107)
  AUTHORS   Tummala,H., Walne,A.J., Williams,M., Bockett,N., Collopy,L.,
            Cardoso,S., Ellison,A., Wynn,R., Leblanc,T., Fitzgibbon,J.,
            Kelsell,D.P., van Heel,D.A., Payne,E., Plagnol,V., Dokal,I. and
            Vulliamy,T.
  TITLE     DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome
            to Corruption in 60S Ribosome Subunit Maturation
  JOURNAL   Am J Hum Genet 99 (1), 115-124 (2016)
   PUBMED   27346687
  REMARK    GeneRIF: findings demonstrate that mutations in DNAJC21 cause a
            cancer-prone bone marrow failure syndrome due to corruption of
            early nuclear rRNA biogenesis and late cytoplasmic maturation of
            the 60S subunit
REFERENCE   6  (bases 1 to 6107)
  AUTHORS   Miyamoto-Sato,E., Fujimori,S., Ishizaka,M., Hirai,N., Masuoka,K.,
            Saito,R., Ozawa,Y., Hino,K., Washio,T., Tomita,M., Yamashita,T.,
            Oshikubo,T., Akasaka,H., Sugiyama,J., Matsumoto,Y. and Yanagawa,H.
  TITLE     A comprehensive resource of interacting protein regions for
            refining human transcription factor networks
  JOURNAL   PLoS One 5 (2), e9289 (2010)
   PUBMED   20195357
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6107)
  AUTHORS   Kaltenbach,L.S., Romero,E., Becklin,R.R., Chettier,R., Bell,R.,
            Phansalkar,A., Strand,A., Torcassi,C., Savage,J., Hurlburt,A.,
            Cha,G.H., Ukani,L., Chepanoske,C.L., Zhen,Y., Sahasrabudhe,S.,
            Olson,J., Kurschner,C., Ellerby,L.M., Peltier,J.M., Botas,J. and
            Hughes,R.E.
  TITLE     Huntingtin interacting proteins are genetic modifiers of
            neurodegeneration
  JOURNAL   PLoS Genet 3 (5), e82 (2007)
   PUBMED   17500595
REFERENCE   8  (bases 1 to 6107)
  AUTHORS   Qiu,X.B., Shao,Y.M., Miao,S. and Wang,L.
  TITLE     The diversity of the DnaJ/Hsp40 family, the crucial partners for
            Hsp70 chaperones
  JOURNAL   Cell Mol Life Sci 63 (22), 2560-2570 (2006)
   PUBMED   16952052
  REMARK    Review article
REFERENCE   9  (bases 1 to 6107)
  AUTHORS   Chen,J., Yin,G., Lu,Y., Lou,M., Cheng,H., Ni,X., Hu,G., Luo,C.,
            Ying,K., Xie,Y. and Mao,Y.
  TITLE     Cloning and characterization of a novel human cDNA encoding a
            J-domain protein (DNAJA5) from the fetal brain
  JOURNAL   Int J Mol Med 13 (5), 735-740 (2004)
   PUBMED   15067379
  REMARK    GeneRIF: molecular cloning
REFERENCE   10 (bases 1 to 6107)
  AUTHORS   Nelson,A. and Myers,K.
  TITLE     Shwachman-Diamond Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301722
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026801.7, BC065745.1,
            AY258422.1 and AC008795.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 11, 2018 this sequence version replaced NM_001012339.2.
            
            Summary: This gene encodes a member of the DNAJ heat shock protein
            40 family of proteins that is characterized by two N-terminal
            tetratricopeptide repeat domains and a C-terminal DNAJ domain. This
            protein binds the precursor 45S ribosomal RNA and may be involved
            in early nuclear ribosomal RNA biogenesis and maturation of the 60S
            ribosomal subunit. Mutations in this gene result in Bone marrow
            failure syndrome 3. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Feb 2017].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, as compared to variant 1. The encoded isoform (2)
            lacks an internal segment, as compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.89834.1,
                                           SRR1660809.2077.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000648817.1/
                                                ENSP00000497410.1
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AC026801.7         171697-171818
            123-567             BC065745.1         99-543
            568-1786            AY258422.1         537-1755
            1787-6107           AC008795.7         48467-52787         c
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.2"
     gene            1..6107
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="DnaJ heat shock protein family (Hsp40) member C21"
                     /db_xref="GeneID:134218"
                     /db_xref="HGNC:HGNC:27030"
                     /db_xref="MIM:617048"
     exon            1..358
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..1857
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="isoform 2 is encoded by transcript variant 2; JJJ1
                     DnaJ domain protein homolog; dnaJ homolog subfamily C
                     member 21; DnaJ homology subfamily A member 5; dnaJ
                     homolog subfamily A member 5; DnaJ (Hsp40) homolog,
                     subfamily C, member 21"
                     /codon_start=1
                     /product="dnaJ homolog subfamily C member 21 isoform 2"
                     /protein_id="NP_001012339.2"
                     /db_xref="CCDS:CCDS34144.1"
                     /db_xref="GeneID:134218"
                     /db_xref="HGNC:HGNC:27030"
                     /db_xref="MIM:617048"
                     /translation="MKCHYEALGVRRDASEEELKKAYRKLALKWHPDKNLDNAAEAAE
                     QFKLIQAAYDVLSDPQERAWYDNHREALLKGGFDGEYQDDSLDLLRYFTVTCYSGYGD
                     DEKGFYTVYRNVFEMIAKEELESVLEEEVDDFPTFGDSQSDYDTVVHPFYAYWQSFCT
                     QKNFAWKEEYDTRQASNRWEKRAMEKENKKIRDKARKEKNELVRQLVAFIRKRDKRVQ
                     AHRKLVEEQNAEKARKAEEMRRQQKLKQAKLVEQYREQSWMTMANLEKELQEMEARYE
                     KEFGDGSDENEMEEHELKDEEDGKDSDEAEDAELYDDLYCPACDKSFKTEKAMKNHEK
                     SKKHREMVALLKQQLEEEEENFSRPQIDENPLDDNSEEEMEDAPKQKLSKKQKKKKQK
                     PAQNYDDNFNVNGPGEGVKVDPEDTNLNQDSAKELEDSPQENVSVTEIIKPCDDPKSE
                     AKSVPKPKGKKTKDMKKPVRVPAEPQTMSVLISCTTCHSEFPSRNKLFDHLKATGHAR
                     APSSSSLNSATSSQSKKEKRKNR"
     exon            359..452
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..576
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..699
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..1004
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1156
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1244
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1403
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1446
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1619
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1695
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..6107
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2154..2159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="hexamer: ATTACA"
     polyA_site      2177
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="major polyA site"
     polyA_site      2347
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
     regulatory      6085..6090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="hexamer: AATAAA"
     polyA_site      6107
                     /gene="DNAJC21"
                     /gene_synonym="BMFS3; DNAJA5; GS3; JJJ1"
                     /note="major polyA site"
ORIGIN      
        1 atgcgaagcg gcggccgccg gcaccgcccc cgccgcgctc ccgcttgccg cagcctgcgt
       61 cgtctgccgc caccgcgcgc cttcactgac ctacaccacc gccgccgccg ccgccgccgc
      121 cgggctcgct ggctggcccg gtgcgggcgg cggactcccg ccggagagga ctgccagcgc
      181 cgccgccgcc gccgcttcgg cccgggcccg ggccccgacc ccgtcccggg ccccagcgcc
      241 ggccgcccgc ccggtcgggc gatgaagtgt cactatgagg cgctgggggt gcggcgcgac
      301 gccagcgagg aggagctcaa gaaggcctat cggaagctgg ccctgaaatg gcacccggat
      361 aaaaatctgg ataatgccgc agaagcagct gaacaattta aattaatcca agcagcatat
      421 gatgtgttga gtgaccctca ggaaagagca tggtatgata atcatagaga ggccctactt
      481 aaaggtgggt ttgatggcga atatcaagat gacagcttag atttgctacg ctatttcacc
      541 gttacctgtt attctggtta tggagatgat gaaaagggat tttacacggt gtatcgtaat
      601 gtttttgaaa tgattgccaa ggaagaacta gaatctgtgt tagaggaaga ggttgatgat
      661 ttcccaactt ttggagactc ccagagtgac tatgatacgg tagtccatcc tttctacgct
      721 tattggcaga gtttctgcac tcaaaagaat tttgcatgga aggaagaata tgatacacga
      781 caggcttcaa accgctggga aaaacgagcc atggaaaaag aaaacaaaaa gattcgggac
      841 aaagcaagga aagagaagaa tgagcttgtc cgtcagctgg tagctttcat tcgtaaaaga
      901 gataaaagag tgcaggcgca tcgaaaactt gtggaagaac agaatgcaga gaaggcgagg
      961 aaagccgaag agatgaggcg gcagcagaag ctaaagcagg ccaaactggt ggagcagtac
     1021 agagaacaga gctggatgac tatggccaat ttggagaaag agctccagga gatggaggca
     1081 cggtacgaga aggagtttgg agatggatcg gatgaaaatg aaatggaaga acatgaactc
     1141 aaagatgagg aggatggtaa agacagtgat gaggccgagg acgctgagct ctatgatgac
     1201 ctttactgcc cagcatgtga caaatcgttc aagacagaaa aggccatgaa gaatcacgag
     1261 aagtcaaaga agcatcggga aatggtggcc ttgctaaaac aacagctgga ggaggaagaa
     1321 gaaaattttt caagacctca aattgatgaa aatccattag atgacaattc tgaggaagaa
     1381 atggaagatg caccaaaaca aaagctttct aaaaaacaga agaaaaagaa acagaaacca
     1441 gcacagaatt atgatgacaa tttcaatgta aatggacctg gagaaggagt aaaggttgat
     1501 ccagaagata ctaacttaaa tcaagacagt gccaaagaat tggaagatag tccccaggaa
     1561 aatgtcagtg tcacagagat cattaaacca tgtgatgatc caaaaagtga agctaaaagt
     1621 gttcctaaac ccaaaggaaa gaaaaccaaa gatatgaaaa aacctgtcag agtacctgct
     1681 gaaccacaaa caatgagtgt tcttatcagc tgtacaacct gccatagtga atttccatct
     1741 cggaataaac tttttgacca tctaaaggcc acaggtcatg caagagcacc ttcatcatcg
     1801 tctttaaaca gcgcaacaag tagtcaaagc aagaaagaga aacgtaaaaa cagatagaga
     1861 ttctgcctgt gcttttgttt gactgtctct agattttgaa accaaaaaac tgaactgaaa
     1921 tcatctaaag agttaaaatt tcagtgatct gcaattaatt acattgtgga agattatttt
     1981 ttatcttgta aaaacacttt tttggtttaa tatatatttt taaaacattt cactagtgat
     2041 tgaattctac ttttgccatc tgaattgact tgaatgtctt aaaacaggta aatactgtaa
     2101 agtgtgtatt cttgatgttt attggctcat gtggacagaa atgtacaggg agaattacat
     2161 tattttaaca cacagaagtg caactttctg ctttattttc tgaatttcac attactttta
     2221 cttaatgctt ttgtgttttg ttaatacttc ataatatgtg aaaaactcgg atcttttaaa
     2281 aagcatcata gatcattttt ccatatgaca ctggttccga ttttaaaaat tatttttaaa
     2341 taaccgatta ttgattactg tatttttttt ctcaagaaca gtgataggta gaaactaatt
     2401 gaacatttgg tagtctttca agaatagtgt ctcttcaagg ttttacttga tttaatttga
     2461 tattttactg gtttaccagt aaggtgtatt gttcagtttt ttgctccgat ttgaattgtg
     2521 gaggtggaag caaattagtt tacatggcat gtcctcccta ggcacagtga cagctgtaaa
     2581 gtatgacgga acaaggtagc agatggtaca gaatttatac tatttaagaa gagatgtggt
     2641 gttcttcatt gagttttttt cttcactatt ttcagaagtt tttgttcttt tttttttttc
     2701 catcactcag tggagaaaag ctttgttaat gaaagatttt gtgatagagc tgatgcttat
     2761 acatctattc tataacagtg atgaatatta acacaaagga aatatggaga actgttataa
     2821 ctgagtgtta gaacagtcgt gtacattgat cagtattgag tccattttta ggatggatta
     2881 gtcagttctt ttcattggtc ttgaaagtaa ttttggctgg tcgcggtggc tcacgcctgt
     2941 aatcccagca ctttgggagg ccgaggcggg cggatcacga ggtcaggaga tcgagaccat
     3001 cccggctaaa acggtgaaac cccgtctcta ctaaaaatac aaaaaattag ccgggcgtag
     3061 tggcgggcgc ctgtagtccc agctacttgg gaggctgagg caggagaatg gcgtgaaccc
     3121 gggaggcgga gcttgcagtg agccgagatc ccgccactgc actccagcct gggcgacaga
     3181 gcgagactcc gtctcaaaaa aaaaaaaaaa aaaaaaaaga aagtaatttt aaactcactg
     3241 gcttgaaatg tgtgctttct gtacaatgaa gttattgctt tccatatatc cttgaatctt
     3301 cagattaacg aaatgaacaa attttcggtt acagatcagc tctcaaacac taaagtcctt
     3361 aatggcaagg tcttttctga tagcttcatg gatagtattg atgatcagat ttcttctgtg
     3421 ttattgtctt aaaaataagc atttgaacct taatttaaat tcttttcttt tccatttaga
     3481 gatatgtttt tttctttacc ttcatacctt tctatttctt gctaatttct atcactaatt
     3541 ccttataatt gtccctgctc cccttcttga tctcttcagg gggaatacta ggcagttttg
     3601 tatcttctga tctcgtaccc tgagacttca tctgaatgtg ctctgccgcc ttcttatctg
     3661 aactagatga attggccata attatgaata ggaatattaa tgaaccagag gacatactag
     3721 tcacatgtta ttatacacta aaaaatagga agtcatcttg aaaggcagtt aatgtactgc
     3781 aaatgtccct atgacttctt tgctttctct tataccaaac atgcaggtat ttaatttgtt
     3841 taatgagggt tgagtggagt tatgtgtcgc ttttattcgg attgacgaaa atcaaccata
     3901 attttcaagg gatcttcatg ggttgtgact ggaagctgac cttagtcttg cgtctcatcc
     3961 tttatgatgt tgagaaatca tctaatttct cagaactttt tcttatctct aacacagtta
     4021 ttaccataat aatattgtct ccatttatca tacaggatca ttatgaaagt aaaatgaagt
     4081 agtatatatg aaaatgcttt ttaaacacaa aagctttata caaaatattg ttgaatttaa
     4141 ataaggtaaa tcttttttag tttatatatt tgcagactat acatgttgga ttaaatgggc
     4201 aattaagttt tataggaatt tgcttcctaa gtctactttt gaagaggaaa acaggaacgt
     4261 accatatatt ttgctaaggg taaaatattt ttggtgaatt gcagacattt aatttcactt
     4321 gtctgaggta aaacaataaa ctaaaataca tgggcatgca tcttacactg atcttattag
     4381 aggtcagtgc taagtaccca tcttgattga agttagctca gatgtggaga ttcatagaat
     4441 gagggtccag gagaagaaag ttaggggaaa tctcattttc tttctgtaat tcattcagtg
     4501 gtgccaaagc tgggggttcc ctacaatggc tatttagact ttccctgctt caaaataaga
     4561 gcagtatcct tagctctagc caagcatttt tctaattcct gcctttggtc acaaagaagg
     4621 aaaagcagag ctgtgaatga gttagtggag gtcaagtcac atcagagttc atgacctagt
     4681 gattgctggt gaagtaatat tggaattttg gtaccatgag aagacttata aaggatttca
     4741 tcagaagttt tcattttttc taaatcctcc cctacaaaat tttcagattg gaaattactc
     4801 ttgtatttgt agaagattgt ctctaaaatt gtggtttaac tcacgcagga agtaaaattc
     4861 ctatagcaag acatagtttc attttagagg acccccaaaa tcccgtgaat tctctggtga
     4921 tgattctagc ctaaccttca acataaaata atgaagagaa cttttaaact tttttatgaa
     4981 ttcctaaaca ttaccattta tgtggcagaa gttctgttcc ctataatctg caatgctgaa
     5041 gtatctctat tcaattccct tccttatctg ttaaatttaa tgtctttata ctaaaaatat
     5101 aggctcttgg gattggagag gaaaaattag aggtgatttt cagatttctt gttctatgga
     5161 agaggaagct gagctcaggg agatatgagc tccacagcca cagctggtta ggggcggctg
     5221 ccagtagaac agggcagatg cctggactcc caactcagta ggccttctgt tacacctcaa
     5281 aatgtgttac atgggaagaa aaagattaag aaaaagcaag ataattttga agaaggacga
     5341 gaggaagagg catgaacttg tcctaacaat gtcaaagaat attttaaagg tataccagtt
     5401 aaagtagtgt gtggctggtg ccagtagagg tcagtcaagg gatccctgac gagaataaag
     5461 agcccagaaa caggtccatg tttatatggg aacttgattt ttgacagata cggcaagctc
     5521 tatggggaaa acaatatgta ctaccttata cccttcccca aaaacaaagg gattaaagac
     5581 ctaaatatga agacaaaact tttaaatgag aatctataag gttattttca tgattttgca
     5641 aatgggaagg atttctttaa agcaagacac aaaagcatac acaggaaaaa atgatgcata
     5701 aaaacatggc ttctgtagaa caatagagac tgtaaaagaa caagctacag acaaggaaat
     5761 gtttgcaatg catggaactg tcaaaggatt cgtatctgga atacattaaa gaagcaaaag
     5821 aacctcatac aaaagtcatt gagtaaatga cagccatgca ttagaacagc aattctcaaa
     5881 acttttggtc tcaagatccc tttacactct tcaaaagtga gaaccccaaa gagttttcat
     5941 gtatgtgggt taaatctatt gatattttct gttagaattt aaaaattatg ttaaaaaaat
     6001 aagaagaatg actatttttc aaaacaaaaa atgagaagag tggtattgtt ttacattttt
     6061 acaaatctct ttaacattta gcttaataaa agatagctgg cctctca
//
