LOCUS       NM_153330               2112 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B8
            (DNAJB8), mRNA.
ACCESSION   NM_153330
VERSION     NM_153330.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ryder,B.D., Ustyantseva,E., Boyer,D.R., Mendoza-Oliva,A.,
            Kuska,M.I., Wydorski,P.M., Macierzynska,P., Morgan,N., Sawaya,M.R.,
            Diamond,M.I., Kampinga,H.H. and Joachimiak,L.A.
  TITLE     DNAJB8 oligomerization is mediated by an aromatic-rich motif that
            is dispensable for substrate activity
  JOURNAL   Structure 32 (6), 662-678 (2024)
   PUBMED   38508190
  REMARK    GeneRIF: DNAJB8 oligomerization is mediated by an aromatic-rich
            motif that is dispensable for substrate activity.
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wang,Z., Li,Y., Mao,R., Zhang,Y., Wen,J., Liu,Q., Liu,Y. and
            Zhang,T.
  TITLE     DNAJB8 in small extracellular vesicles promotes Oxaliplatin
            resistance through TP53/MDR1 pathway in colon cancer
  JOURNAL   Cell Death Dis 13 (2), 151 (2022)
   PUBMED   35165262
  REMARK    GeneRIF: DNAJB8 in small extracellular vesicles promotes
            Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2112)
  AUTHORS   Huusko,J.M., Tiensuu,H., Haapalainen,A.M., Pasanen,A.,
            Tissarinen,P., Karjalainen,M.K., Zhang,G., Christensen,K.,
            Ryckman,K.K., Jacobsson,B., Murray,J.C., Kingsmore,S.F.,
            Hallman,M., Muglia,L.J. and Ramet,M.
  TITLE     Integrative genetic, genomic and transcriptomic analysis of heat
            shock protein and nuclear hormone receptor gene associations with
            spontaneous preterm birth
  JOURNAL   Sci Rep 11 (1), 17115 (2021)
   PUBMED   34429451
  REMARK    GeneRIF: Integrative genetic, genomic and transcriptomic analysis
            of heat shock protein and nuclear hormone receptor gene
            associations with spontaneous preterm birth.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2112)
  AUTHORS   Ryder,B.D., Matlahov,I., Bali,S., Vaquer-Alicea,J., van der
            Wel,P.C.A. and Joachimiak,L.A.
  TITLE     Regulatory inter-domain interactions influence Hsp70 recruitment to
            the DnaJB8 chaperone
  JOURNAL   Nat Commun 12 (1), 946 (2021)
   PUBMED   33574241
  REMARK    GeneRIF: Regulatory inter-domain interactions influence Hsp70
            recruitment to the DnaJB8 chaperone.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2112)
  AUTHORS   Nishizawa,S., Hirohashi,Y., Torigoe,T., Takahashi,A., Tamura,Y.,
            Mori,T., Kanaseki,T., Kamiguchi,K., Asanuma,H., Morita,R.,
            Sokolovskaya,A., Matsuzaki,J., Yamada,R., Fujii,R., Kampinga,H.H.,
            Kondo,T., Hasegawa,T., Hara,I. and Sato,N.
  TITLE     HSP DNAJB8 controls tumor-initiating ability in renal cancer
            stem-like cells
  JOURNAL   Cancer Res 72 (11), 2844-2854 (2012)
   PUBMED   22552285
  REMARK    GeneRIF: Cancer stem-like cell (CSC) maintenance and that it offers
            a candidate for CSC-targeting immunotherapy in renal cell carcinoma
REFERENCE   6  (bases 1 to 2112)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   7  (bases 1 to 2112)
  AUTHORS   Hageman,J., van Waarde,M.A., Zylicz,A., Walerych,D. and
            Kampinga,H.H.
  TITLE     The diverse members of the mammalian HSP70 machine show distinct
            chaperone-like activities
  JOURNAL   Biochem J 435 (1), 127-142 (2011)
   PUBMED   21231916
REFERENCE   8  (bases 1 to 2112)
  AUTHORS   Heldens,L., Dirks,R.P., Hensen,S.M., Onnekink,C., van Genesen,S.T.,
            Rustenburg,F. and Lubsen,N.H.
  TITLE     Co-chaperones are limiting in a depleted chaperone network
  JOURNAL   Cell Mol Life Sci 67 (23), 4035-4048 (2010)
   PUBMED   20556630
REFERENCE   9  (bases 1 to 2112)
  AUTHORS   Hageman,J., Rujano,M.A., van Waarde,M.A., Kakkar,V., Dirks,R.P.,
            Govorukhina,N., Oosterveld-Hut,H.M., Lubsen,N.H. and Kampinga,H.H.
  TITLE     A DNAJB chaperone subfamily with HDAC-dependent activities
            suppresses toxic protein aggregation
  JOURNAL   Mol Cell 37 (3), 355-369 (2010)
   PUBMED   20159555
  REMARK    GeneRIF: DNAJB6b and DNAJB8 are superior suppressors of aggregation
            and toxicity of disease-associated polyglutamine proteins.
REFERENCE   10 (bases 1 to 2112)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from KF459586.1 and AC080005.24.
            
            On May 1, 2021 this sequence version replaced NM_153330.5.
            
            Summary: The protein encoded by this gene belongs to the DNAJ/HSP40
            family of proteins that regulate chaperone activity. This family
            member suppresses aggregation and toxicity of polyglutamine
            proteins, and the C-terminal tail is essential for this activity.
            It has been implicated as a cancer-testis antigen and as a cancer
            stem-like cell antigen involved in renal cell carcinoma. [provided
            by RefSeq, Jun 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050288.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319153.4/ ENSP00000316053.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                KF459586.1         330-344
            16-277              AC080005.24        33860-34121         c
            278-438             AC080005.24        32522-32682         c
            439-2112            AC080005.24        29683-31356         c
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..2112
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /note="DnaJ heat shock protein family (Hsp40) member B8"
                     /db_xref="GeneID:165721"
                     /db_xref="HGNC:HGNC:23699"
                     /db_xref="MIM:611337"
     exon            1..277
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /inference="alignment:Splign:2.1.0"
     exon            278..438
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /inference="alignment:Splign:2.1.0"
     exon            439..2112
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /inference="alignment:Splign:2.1.0"
     CDS             1304..2002
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /note="DnaJ (Hsp40) homolog, subfamily B, member 8;
                     testicular tissue protein Li 56"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 8"
                     /protein_id="NP_699161.1"
                     /db_xref="CCDS:CCDS3048.1"
                     /db_xref="GeneID:165721"
                     /db_xref="HGNC:HGNC:23699"
                     /db_xref="MIM:611337"
                     /translation="MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEK
                     KFKLVSEAYEVLSDSKKRSLYDRAGCDSWRAGGGASTPYHSPFDTGYTFRNPEDIFRE
                     FFGGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGEFPAFMEAFSSFNMLGCSGGS
                     HTTFSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVEEDGQLKSV
                     TVNGKEQLKWMDSK"
     regulatory      2090..2095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /note="hexamer: AATAAA"
     polyA_site      2112
                     /gene="DNAJB8"
                     /gene_synonym="CT156; DJ6"
                     /note="major polyA site"
ORIGIN      
        1 agcggctctc tagagcttgc ctgggagctg ctgaggagct cacggtattc caggaagctc
       61 cccatccatg cctcagcctg tggctcagaa gcagggtctt cgcagaagat tgccccgagc
      121 tgttgcaaag ctcaccattg tcacctgcct gcaacggcct ctcttttcca ctctccaaat
      181 cccttgtatt ggcaggctct agtctggaat tataccggtg atgggattct ggaaaatccc
      241 tcagatttct ccaggatgca aggagaccat ggaagaggaa caagcctgca agggacagca
      301 ggtgctaact gctaaccgag acatagtgac aaaaatccag ccacagagat aaagaatcag
      361 gttcatctgt agctacatcc agtggagaag ttctgctctt caactcctct gctaagccac
      421 atgtgtcagc aggtgtagaa ttgagtggaa cacttctttg gatttgctgt ccgctgcagc
      481 cttggccctg gtttcacatc ccctccctca ctgactcgct gtgtgacctt gagcactgtg
      541 tgtctctgag cctggttcat ccaagagttt accgacggcc ctcgtgtgcc agccactgtc
      601 ctagacaggg acagactttc ccgcctttgt ggagcttacc ccggaagacc tggccagata
      661 atgggcacaa aaaaagcagg ctcttgtccc caccccagcc tgcctgaacc ccaccttggc
      721 ctcctcttgc ctcagctgct gcccagccat cggccgagtg gacataccca ggacgccctt
      781 gcccgcctcc cccaccacgg ccacagacct ttgtcactca ccaaggctgg aatcccgtgg
      841 ctgatcctgc ctctgccctt tggctcccgg gactcagccc acaccacctg ggtcacagag
      901 catcccattc ccacacaccg ttgtggccac ctcaccagca ggggcaggcc catatgccca
      961 ggtttgcctg gtgaggagct gggggcgggg gtatgccccg ccccgggagc tgacgtcata
     1021 aaaggagctc tggagggcag cccactctgg cctggcccca cagcggcagt gtccctcccc
     1081 tccccccact cctctcagtg ggggcccctc cagtccctga gaattggtac tacgaaaagg
     1141 tgaactcctg ggcagaatct tgcctagagc ttgcggagtc cagccaggcc cctgctgaag
     1201 ggccccagac caccggccac ttctcccccg tccatctgac cagctgggcc cctgcgccca
     1261 cctggcctcc acgttccctc tcctctcacc cacacccctg gccatggcta actactacga
     1321 agtgctgggc gtgcaggcca gcgcttcccc ggaggacatc aagaaagcct accgcaagct
     1381 ggcccttcgt tggcaccccg acaagaaccc tgacaataag gaggaggcgg agaagaagtt
     1441 caagctggtg tctgaggcct atgaggttct gtctgactcc aagaaacgct ccctgtatga
     1501 ccgtgctggc tgtgacagct ggcgggctgg tggcggggcc agcacgccct accacagccc
     1561 cttcgacacc ggctacacct tccgtaaccc tgaggacatc ttccgggagt tttttggtgg
     1621 cctggaccct ttctcctttg agttctggga cagcccattc aatagtgacc gtggtggccg
     1681 gggccatggc ctgagggggg ccttctcggc aggctttgga gaatttccgg ccttcatgga
     1741 ggccttctca tccttcaaca tgctgggctg cagcgggggc agccacacca ccttctcatc
     1801 cacctccttc gggggctcca gttctggcag ctcggggttc aagtcggtga tgtcgtccac
     1861 cgagatgatc aatggccaca aggtcaccac caagcgcatc gtggagaacg ggcaggagcg
     1921 cgtggaggtg gaggaagacg ggcagctcaa gtcggtgact gtgaacggca aggagcagct
     1981 caaatggatg gacagcaagt aggcgctggc cacccggccc tgccttccca ccaccaccac
     2041 cgtgcatggg gcagcaaaca cgtggggccg cagacatagc ctgatggtta ataaatgtgc
     2101 caagtgagtt ca
//
