LOCUS       NM_001349723            2651 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B5
            (DNAJB5), transcript variant 4, mRNA.
ACCESSION   NM_001349723
VERSION     NM_001349723.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Pang,M., Xiong,C., Xiao,C., Du,J., Zheng,L., Bai,L., Zhu,X. and
            Xiong,J.W.
  TITLE     Critical role of zebrafish dnajb5 in myocardial proliferation and
            regeneration
  JOURNAL   J Genet Genomics 47 (8), 493-496 (2020)
   PUBMED   33191150
  REMARK    GeneRIF: Critical role of zebrafish dnajb5 in myocardial
            proliferation and regeneration.
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Sterrenberg,J.N., Blatch,G.L. and Edkins,A.L.
  TITLE     Human DNAJ in cancer and stem cells
  JOURNAL   Cancer Lett 312 (2), 129-142 (2011)
   PUBMED   21925790
  REMARK    Review article
REFERENCE   3  (bases 1 to 2651)
  AUTHORS   Hageman,J., van Waarde,M.A., Zylicz,A., Walerych,D. and
            Kampinga,H.H.
  TITLE     The diverse members of the mammalian HSP70 machine show distinct
            chaperone-like activities
  JOURNAL   Biochem J 435 (1), 127-142 (2011)
   PUBMED   21231916
REFERENCE   4  (bases 1 to 2651)
  AUTHORS   Zijlstra,M.P., Rujano,M.A., Van Waarde,M.A., Vis,E., Brunt,E.R. and
            Kampinga,H.H.
  TITLE     Levels of DNAJB family members (HSP40) correlate with disease onset
            in patients with spinocerebellar ataxia type 3
  JOURNAL   Eur J Neurosci 32 (5), 760-770 (2010)
   PUBMED   20726892
REFERENCE   5  (bases 1 to 2651)
  AUTHORS   Hageman,J., Rujano,M.A., van Waarde,M.A., Kakkar,V., Dirks,R.P.,
            Govorukhina,N., Oosterveld-Hut,H.M., Lubsen,N.H. and Kampinga,H.H.
  TITLE     A DNAJB chaperone subfamily with HDAC-dependent activities
            suppresses toxic protein aggregation
  JOURNAL   Mol Cell 37 (3), 355-369 (2010)
   PUBMED   20159555
REFERENCE   6  (bases 1 to 2651)
  AUTHORS   Ago,T., Liu,T., Zhai,P., Chen,W., Li,H., Molkentin,J.D.,
            Vatner,S.F. and Sadoshima,J.
  TITLE     A redox-dependent pathway for regulating class II HDACs and cardiac
            hypertrophy
  JOURNAL   Cell 133 (6), 978-993 (2008)
   PUBMED   18555775
REFERENCE   7  (bases 1 to 2651)
  AUTHORS   Forsman,A., Ruetschi,U., Ekholm,J. and Rymo,L.
  TITLE     Identification of intracellular proteins associated with the
            EBV-encoded nuclear antigen 5 using an efficient TAP procedure and
            FT-ICR mass spectrometry
  JOURNAL   J Proteome Res 7 (6), 2309-2319 (2008)
   PUBMED   18457437
REFERENCE   8  (bases 1 to 2651)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
REFERENCE   9  (bases 1 to 2651)
  AUTHORS   Chen,M.S., Roti,J.R. and Laszlo,A.
  TITLE     Hsc40, a new member of the hsp40 family, exhibits similar
            expression profile to that of hsc70 in mammalian cells
  JOURNAL   Gene 238 (2), 333-341 (1999)
   PUBMED   10570961
REFERENCE   10 (bases 1 to 2651)
  AUTHORS   Cheetham,M.E. and Caplan,A.J.
  TITLE     Structure, function and evolution of DnaJ: conservation and
            adaptation of chaperone function
  JOURNAL   Cell Stress Chaperones 3 (1), 28-36 (1998)
   PUBMED   9585179
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL355377.25.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 5, 2021 this sequence version replaced NM_001349723.2.
            
            Summary: This gene encodes a member of the DNAJ heat shock protein
            40 family of co-chaperone proteins. The encoded protein contains an
            N-terminal DNAJ domain and a C-terminal substrate binding domain
            but lacks the cysteine-rich domain found in other DNAJ family
            members. In mice, a multi-protein complex containing this protein,
            thioredoxin 1, and histone deacetylase 4, serves as a master
            negative regulator of cardiac hypertrophy. [provided by RefSeq, Mar
            2017].
            
            Transcript Variant: This variant (4) differs in the 5' UTR,
            compared to variant 1. Both variants 1 and 4 encode the same
            isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.2570080.1,
                                           SRR1660809.181628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2153980
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682809.1/ ENSP00000507741.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AL355377.25        114503-114589
            88-401              AL355377.25        115257-115570
            402-646             AL355377.25        117958-118202
            647-1248            AL355377.25        121023-121624
            1249-2651           AL355377.25        121784-123186
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..2651
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /note="DnaJ heat shock protein family (Hsp40) member B5"
                     /db_xref="GeneID:25822"
                     /db_xref="HGNC:HGNC:14887"
                     /db_xref="MIM:611328"
     exon            1..87
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /inference="alignment:Splign:2.1.0"
     exon            88..401
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..1482
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /note="isoform 1 is encoded by transcript variant 4; heat
                     shock cognate 40; dnaJ homolog subfamily B member 5; heat
                     shock protein Hsp40-2; heat shock protein Hsp40-3; heat
                     shock protein cognate 40; DnaJ (Hsp40) homolog, subfamily
                     B, member 5"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 5 isoform 1"
                     /protein_id="NP_001336652.1"
                     /db_xref="CCDS:CCDS47959.1"
                     /db_xref="GeneID:25822"
                     /db_xref="HGNC:HGNC:14887"
                     /db_xref="MIM:611328"
                     /translation="MFKRTVLSCPPPAAPPLQARGAFRSFPHSWGEDFLASLMFKIQL
                     EPLKLRAWTLNGFVKFRNKETSAGPVAVMGKDYYKILGIPSGANEDEIKKAYRKMALK
                     YHPDKNKEPNAEEKFKEIAEAYDVLSDPKKRGLYDQYGEEGLKTGGGTSGGSSGSFHY
                     TFHGDPHATFASFFGGSNPFDIFFASSRSTRPFSGFDPDDMDVDEDEDPFGAFGRFGF
                     NGLSRGPRRAPEPLYPRRKVQDPPVVHELRVSLEEIYHGSTKRMKITRRRLNPDGRTV
                     RTEDKILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVL
                     YSALISLKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGD
                     LIVEFKVRFPDRLTPQTRQILKQHLPCS"
     exon            402..646
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /inference="alignment:Splign:2.1.0"
     exon            647..1248
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..2651
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2632..2637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /note="hexamer: AATAAA"
     polyA_site      2651
                     /gene="DNAJB5"
                     /gene_synonym="Hsc40"
                     /note="major polyA site"
ORIGIN      
        1 gtcccgggtg gaggcggcgg agccggagcc gggggagggg gcagcggctg tctcacggac
       61 cacggcggcg cccgcagctc ctcaccggtc cggcacctgc tgcgccagac aggccttgct
      121 gggcacgcgc ctctgagagt ccaaggaggt ggcttccaga gctgcagcac ttcaggccgg
      181 ctccggtgga gcgatcagag gtgaggggct tggctgggca tgtttaagcg cacagtgctc
      241 tcctgcccac ccccagcagc acccccactg caggcccgag gagctttccg gagcttccca
      301 cactcctggg gagaagactt cttagccagc ttgatgttta aaattcagct ggagccctta
      361 aaacttcgag cgtggacgct gaatgggttt gtaaagtttc gaaacaagga gaccagtgct
      421 ggtccagtgg ctgtgatggg aaaagattat tacaagattc ttgggatccc atcgggggcc
      481 aacgaggatg agatcaagaa agcctaccgg aagatggcct tgaagtacca cccagacaag
      541 aataaagaac ccaacgctga ggagaagttt aaggagattg cagaggccta tgatgtgcta
      601 agtgacccca agaaacgggg cctgtatgac cagtatgggg aggaaggcct gaagaccggc
      661 ggtggcacat caggtggctc cagtggctcc tttcactaca cctttcatgg ggacccccat
      721 gccacctttg cctccttctt tggtggctcc aaccccttcg atatcttctt tgccagcagc
      781 cgctccactc ggcccttcag tggctttgac ccagatgaca tggatgtgga tgaagatgag
      841 gacccatttg gcgctttcgg ccgttttggc ttcaatgggc tgagtagggg tccaaggcga
      901 gccccagaac cactgtaccc tcggcgcaag gtgcaggacc ccccagtggt gcacgagctg
      961 cgggtgtccc tggaggagat ctaccatggc tccaccaagc gcatgaagat cacaaggcgt
     1021 cgcctcaacc ctgatgggcg aactgtgcgc accgaggaca agatcctgca catagtcatc
     1081 aagcgtggct ggaaggaagg caccaagatc accttcccca aagaaggcga cgccacacct
     1141 gacaacatcc ctgctgacat cgtctttgtg ctcaaagaca agccccatgc acacttccgc
     1201 cgagatggca ccaacgtgct ctacagtgcc ctgatcagcc tcaaggaggc gctgtgtggc
     1261 tgcactgtga acattcccac tatcgacggc cgagtgatcc ctttgccctg caatgatgtc
     1321 atcaagccag gcaccgtgaa gagactccgt ggggagggcc ttcccttccc caaagtgcca
     1381 actcagcgag gagacctcat tgttgagttc aaagttcgct tcccagacag attaacacca
     1441 cagacaagac agatccttaa gcagcaccta ccctgttcct aggctctgcc ccagccagtc
     1501 cagagcctac cacagcaata cccccaacac tcactccact caatgtgcac ccagcttgat
     1561 gtccactgga cactggcaac tttttctaaa atgcaaaaaa aagccactgg ttttcaggaa
     1621 aatgttcctg tccctgaccc cttttagagc tgggctgcct ggggggagtg ggagggaggt
     1681 ggggagagct agcccaggcc aggggtcaat gttcatgtca ctagctttca atccagtttc
     1741 cactgcagtg gctggagagt gacctgagtg ctacttgaag atatgtagag attccttatc
     1801 catgcctgta catagcatgt cctcctcccc tcagctttgc taataccagt cccctccctt
     1861 ccctttggct tcctgctgtt gggggtggaa aagactagaa aggacattgc tttctcagcc
     1921 ccacccccag gtccacagtg tccaaggtaa tggcacacat attcatgcac aagaagcact
     1981 cacctagagc tgtctgtgcc tctctgggag aaaggagaga ggataagaag ggaaagttca
     2041 caacctgtga acagggactt gagcacgaga cacttttcat ctggagcagg ggggtgagct
     2101 cctcagcagc ctccgtaaca gctgccctgc ctacacctgc agagctggag gttctgtcct
     2161 ccctgctgct ctcaggagtg ttcaagggtg gagggcaggg aatggggcct gaggtattaa
     2221 ggctaagagg tgggggacag ggcccatcta ccagccttca tcaggaaggg aaagggcttt
     2281 ggggtcaggt ggcagctatt ccccaccaag agattcaggg tcacaggttt ttccccacac
     2341 ctctgaactc agggcctagc cacccccaaa cttccaaatc ccacttttgt gatatgtgaa
     2401 gctactcatt tcttaccctt ggaggctgtg tggggaattt ccagcccttt tatgcctgtg
     2461 tgatcccacc ccacccccat agttgtataa aggtcatagt gaagaagctg ggggaagact
     2521 gcttcagcca gatcctgggg tggggtcttt aggttttctc actttgacaa cccccgaatg
     2581 tttttatagt agtttttttg tatttttttg taatgacagt attatgtaaa aaataaagta
     2641 ttttaaaaat a
//
