LOCUS       NM_006736               3072 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B2
            (DNAJB2), transcript variant 2, mRNA.
ACCESSION   NM_006736
VERSION     NM_006736.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Chen,H., Lin,C., Xue,H.M., Chen,C. and Yang,M.
  TITLE     The heat shock protein DNAJB2 as a novel biomarker for essential
            thrombocythemia diagnosis associated with immune infiltration
  JOURNAL   Thromb Res 223, 131-138 (2023)
   PUBMED   36746103
  REMARK    GeneRIF: The heat shock protein DNAJB2 as a novel biomarker for
            essential thrombocythemia diagnosis associated with immune
            infiltration.
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Saveri,P., Magri,S., Maderna,E., Balistreri,F., Lombardi,R.,
            Ciano,C., Moda,F., Garavaglia,B., Reale,C., Lauria Pinter,G.,
            Taroni,F., Pareyson,D. and Pisciotta,C.
  TITLE     DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism
            insights and phenotypic spectrum widening
  JOURNAL   Eur J Neurol 29 (7), 2056-2065 (2022)
   PUBMED   35286755
  REMARK    GeneRIF: DNAJB2-related Charcot-Marie-Tooth disease type 2:
            Pathomechanism insights and phenotypic spectrum widening.
REFERENCE   3  (bases 1 to 3072)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3072)
  AUTHORS   Wang,Y., Anderson,E.P. and Tatakis,D.N.
  TITLE     Whole transcriptome analysis of smoker palatal mucosa identifies
            multiple downregulated innate immunity genes
  JOURNAL   J Periodontol 91 (6), 756-766 (2020)
   PUBMED   31682009
  REMARK    GeneRIF: DNAJB2 expression in healthy human palatal mucosa is
            strongly negatively correlated with serum cotinine levels
REFERENCE   5  (bases 1 to 3072)
  AUTHORS   Chapple,J.P., Hardcastle,A.J., Kurzik-Dumke,U., Collier,D.A. and
            Cheetham,M.E.
  TITLE     Assignment of the neuronal cochaperone, HSJ1, to human chromosome
            bands 2q32-->q34 between D2S295 and D2S339 by in situ hybridization
            and somatic cell and radiation hybrids
  JOURNAL   Cytogenet Cell Genet 86 (1), 62-63 (1999)
   PUBMED   10516435
REFERENCE   6  (bases 1 to 3072)
  AUTHORS   Maheswaran,S., Englert,C., Zheng,G., Lee,S.B., Wong,J.,
            Harkin,D.P., Bean,J., Ezzell,R., Garvin,A.J., McCluskey,R.T.,
            DeCaprio,J.A. and Haber,D.A.
  TITLE     Inhibition of cellular proliferation by the Wilms tumor suppressor
            WT1 requires association with the inducible chaperone Hsp70
  JOURNAL   Genes Dev 12 (8), 1108-1120 (1998)
   PUBMED   9553041
REFERENCE   7  (bases 1 to 3072)
  AUTHORS   Cheetham,M.E., Jackson,A.P. and Anderton,B.H.
  TITLE     Regulation of 70-kDa heat-shock-protein ATPase activity and
            substrate binding by human DnaJ-like proteins, HSJ1a and HSJ1b
  JOURNAL   Eur J Biochem 226 (1), 99-107 (1994)
   PUBMED   7957263
REFERENCE   8  (bases 1 to 3072)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Hereditary Neuropathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301532
REFERENCE   9  (bases 1 to 3072)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 2 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301462
REFERENCE   10 (bases 1 to 3072)
  AUTHORS   Cheetham,M.E., Brion,J.P. and Anderton,B.H.
  TITLE     Human homologues of the bacterial heat-shock protein DnaJ are
            preferentially expressed in neurons
  JOURNAL   Biochem J 284 (Pt 2) (Pt 2), 469-476 (1992)
   PUBMED   1599432
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114803.5, BT007088.1,
            BX478051.1, CA392563.1, BF795662.1 and BC047056.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_006736.5.
            
            Summary: This gene is almost exclusively expressed in the brain,
            mainly in the neuronal layers. It encodes a protein that shows
            sequence similarity to bacterial DnaJ protein and the yeast
            homologs. In bacteria, this protein is implicated in protein
            folding and protein complex dissociation. Alternatively spliced
            transcript variants encoding different isoforms have been described
            for this gene. [provided by RefSeq, Jul 2011].
            
            Transcript Variant: This variant (2) is alternatively spliced at
            the 3' end compared to variant 1. This results in a longer isoform
            (b) with a distinct C-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289626.1, AK312723.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336576.10/ ENSP00000338019.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC114803.5         3464-3616
            154-254             AC114803.5         3896-3996
            255-364             AC114803.5         4676-4785
            365-418             AC114803.5         5816-5869
            419-541             AC114803.5         6037-6159
            542-634             AC114803.5         6935-7027
            635-737             AC114803.5         7231-7333
            738-808             AC114803.5         7517-7587
            809-1012            AC114803.5         8730-8933
            1013-1162           BT007088.1         824-973
            1163-1434           BX478051.1         359-630
            1435-1821           CA392563.1         6-392
            1822-2430           BF795662.1         21-629
            2431-3072           BC047056.1         1131-1772
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..3072
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /note="DnaJ heat shock protein family (Hsp40) member B2"
                     /db_xref="GeneID:3300"
                     /db_xref="HGNC:HGNC:5228"
                     /db_xref="MIM:604139"
     exon            1..153
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            154..254
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..1164
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /note="isoform b is encoded by transcript variant 2; heat
                     shock protein, neuronal DNAJ-like 1; heat shock protein
                     J1; dnaJ protein homolog 1; heat shock 40 kDa protein 3;
                     DnaJ (Hsp40) homolog, subfamily B, member 2"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 2 isoform b"
                     /protein_id="NP_006727.2"
                     /db_xref="CCDS:CCDS2439.1"
                     /db_xref="GeneID:3300"
                     /db_xref="HGNC:HGNC:5228"
                     /db_xref="MIM:604139"
                     /translation="MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEK
                     KFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEV
                     FREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPG
                     AGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLEL
                     SRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGG
                     REAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL"
     exon            255..364
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            365..418
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            419..541
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            542..634
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            635..737
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            738..808
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     exon            809..3072
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3053..3058
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="DNAJB2"
                     /gene_synonym="CMT2T; DSMA5; HMNR5; HSJ-1; HSJ1; HSPF3"
                     /note="major polyA site"
ORIGIN      
        1 agtagtttcc gcaccgctga cgcatgcctg cgcgcacagc tgggccgggc gcgtcctacg
       61 cagcagccgc gagccgggct gcgggtgcca gacggttccc ggcggggggc aggggcgggg
      121 cgccgcagga ggccgggact cctggcggag gagccccaag gaggcccgcc tgacgactga
      181 ccagttgcca tggcatccta ctacgagatc ctagacgtgc cgcgaagtgc gtccgctgat
      241 gacatcaaga aggcgtatcg gcgcaaggct ctccagtggc acccagacaa aaacccagat
      301 aataaagagt ttgctgagaa gaaatttaag gaggtggccg aggcatatga agtgctgtct
      361 gacaagcaca agcgggagat ttacgaccgc tatggccggg aagggctgac agggacagga
      421 actggcccat ctcgggcaga agctggcagt ggtgggcctg gcttcacctt caccttccgc
      481 agccccgagg aggtcttccg ggaattcttt gggagtggag acccttttgc agagctcttt
      541 gatgacctgg gccccttctc agagcttcag aaccggggtt cccgacactc aggccccttc
      601 tttaccttct cttcctcctt ccctgggcac tccgatttct cctcctcatc tttctccttc
      661 agtcctgggg ctggtgcttt tcgctctgtt tctacatcta ccacctttgt ccaaggacgc
      721 cgcatcacca cacgcagaat catggagaac gggcaggagc gggtggaagt ggaggaggat
      781 gggcagctga agtcagtcac aatcaatggt gtcccagatg acctggcact gggcttggag
      841 ctgagccgtc gcgagcagca gccgtcagtc acttccaggt ctgggggcac tcaggtccag
      901 cagacccctg cctcatgccc cttggacagc gacctctctg aggatgagga cctgcagctg
      961 gccatggcct acagcctgtc agagatggag gcagctggga agaaacccgc aggtgggcgg
     1021 gaggcacagc accgacggca ggggcggccc aaggcccagc accaagatcc aggcttgggg
     1081 gggacccagg agggtgcgag gggtgaagca accaaacgca gtccatcccc agaggagaag
     1141 gcctctcgct gcctcatcct ctgaacaccg ggcccaacct gatctgatcc agatcttgac
     1201 tggggggtct gactcactgt gggaagagaa gaggggagta tcctgagttg taggaactgc
     1261 tttccaactc caagctccct ccacaagttt ccctcccagg ccccccacac cccagtgtgg
     1321 acttgggatt tgctgtgctc agcccagggc tgataggtcc ctggtgaagc ccagggtggg
     1381 gggtgtcagg gcagtggagg ggcccgagga gccaggttgc atttattgga tggggagctc
     1441 caaggggcat tagtggtttg ggctgggcct tttgtgccct ggtactctgc cacctgtgtt
     1501 gctgatggtg tcaaggaagg aggacttggc ctagggttgt ctgagccgga gccggcagct
     1561 ccactggaga gcagtgcagg cagagtggag cctcctgctc tcctggacca gctgcagacc
     1621 cccaaccctg gtttctgtgc catgttgcgc tctgaccgtc tctgttgctt ctcttctggt
     1681 gttgcttctc ctccctccca ttctctctgc aactccctgc gggccgcatc gcttgctttc
     1741 actgccgtct ggctaggact cccttcttcc ttccttcccc gagaaggcct caatgtggcg
     1801 aggaagatgc tggggccggt agggctgtga gatcttctgg ggaggctagc cgggtggggc
     1861 gggagcctct cagctgtcca gattcagaac tggagcccac tcctcctccc tctcgttgcc
     1921 tcagcctgcc ctcaccctca ggactaggca gaggtgaggc tggctcaccc tgaagaggtg
     1981 ggataggacc gggggacccc agagggaggc ctaggagggg actgcaccca tactgcttcc
     2041 ctaccacaaa tcagggctca gggagaggcc atgcggccag cccaggtctg catgctgagc
     2101 cccatcctcc acagcttgcc gctgacgctc tctcctgtca ccccgcccct gctctctccc
     2161 cagatgtgtt ctgagctgga tgccgggttc cagaatcgct gcacagttcc aacaggacag
     2221 cgccttcccc catgcgctgg gaggggaccc tccatttctc cccctcaccc atgctgagtg
     2281 tagagccggg gcctgggtgg cgggtggggg ccgggtggga ggtggcagta gtcttagcct
     2341 gtgcactctc ttccttgggt gtttggtgct ggctcctggg gactacaaat cccagagtgc
     2401 ggtgtgcccg gcctcatttc tgatagatcc cgcttggggg aggtggtgta tggttacgga
     2461 gctgtgcatc ttgggacatg tagtagccca ggtcggcttg tcactcgctg tgagatgggg
     2521 agattttgtc ttttgattta tccctgtagg gctggcaggg ttgtagatga agggggaatg
     2581 atctgagcct tggttcccct gacacgtctt gctagcccca gggttagagt gggcagggca
     2641 gagccgcgca gcacctggga gcggtacctt tcccttgggc agcctggggt cccaggaaca
     2701 agccagggcg agtggcatgt ctgcctgagc agggtgtggc cccagaaagc tgaggagtgt
     2761 gggctggcag agagcttcga gggcaaggcc acccgcgggg gcgtgtgtgt ggtggggctt
     2821 ggcatgtgat ggcagctcca ggcatgccgc tgcttgtatg gctttctttg gcctctgacc
     2881 ctgctgccca ttctttccaa catcacagat gaactgcctc tcctcctccc tgcctgggga
     2941 gcccagtggc cagggagggg agtggtggag ccagtcgctg taacactgag cctcagagac
     3001 gaaccaaaac cagctgggct gagctcagat ccagggggaa atgctggaag tcaataaaac
     3061 tgagttttga ga
//
