LOCUS       NM_001031723            5967 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B14
            (DNAJB14), transcript variant 1, mRNA.
ACCESSION   NM_001031723
VERSION     NM_001031723.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Purificacao,A.D.D., Debbas,V., Tanaka,L.Y., Gabriel,G.V.M., Wosniak
            Junior,J., De Bessa,T.C., Garcia-Rosa,S., Laurindo,F.R.M. and
            Oliveira,P.V.S.
  TITLE     DNAJB12 and DNJB14 are non-redundant Hsp40 redox chaperones
            involved in endoplasmic reticulum protein reflux
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (1), 130502 (2024)
   PUBMED   37925033
  REMARK    GeneRIF: DNAJB12 and DNJB14 are non-redundant Hsp40 redox
            chaperones involved in endoplasmic reticulum protein reflux.
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   3  (bases 1 to 5967)
  AUTHORS   Li,K., Jiang,Q., Bai,X., Yang,Y.F., Ruan,M.Y. and Cai,S.Q.
  TITLE     Tetrameric Assembly of K+ Channels Requires ER-Located Chaperone
            Proteins
  JOURNAL   Mol Cell 65 (1), 52-65 (2017)
   PUBMED   27916661
  REMARK    GeneRIF: ER-located J-protein chaperones were identified as key
            regulators for the iogenesis and physiological function of ERG K+
            channels. They regulate two distinct aspects of K+ channel
            biogenesis, the stabilization and assembly of channel subunits.
REFERENCE   4  (bases 1 to 5967)
  AUTHORS   Pankow,S., Bamberger,C., Calzolari,D., Martinez-Bartolome,S.,
            Lavallee-Adam,M., Balch,W.E. and Yates,J.R. 3rd.
  TITLE     F508 CFTR interactome remodelling promotes rescue of cystic
            fibrosis
  JOURNAL   Nature 528 (7583), 510-516 (2015)
   PUBMED   26618866
REFERENCE   5  (bases 1 to 5967)
  AUTHORS   Goodwin,E.C., Motamedi,N., Lipovsky,A., Fernandez-Busnadiego,R. and
            DiMaio,D.
  TITLE     Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the
            nucleus by membranes associated with a novel nuclear pore structure
  JOURNAL   PLoS One 9 (4), e94322 (2014)
   PUBMED   24732912
  REMARK    GeneRIF: Over-expression of DNAJB12 or DNAJB14 causes the formation
            of elaborate membranous structures within cell nuclei, designated
            DJANGOS for DNAJ-associated nuclear globular structures.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5967)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 5967)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA854416.1, BC022248.1, AY358272.1, AC097460.3, AP001962.2 and
            AL121021.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001031723.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.126686.1,
                                           SRR1803615.174620.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000442697.7/ ENSP00000404381.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DA854416.1         109-131
            24-1690             BC022248.1         1-1667
            1691-2624           AY358272.1         1653-2586
            2625-4397           AC097460.3         7475-9247           c
            4398-5313           AP001962.2         1-916
            5314-5967           AL121021.1         14-667              c
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q23"
     gene            1..5967
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /note="DnaJ heat shock protein family (Hsp40) member B14"
                     /db_xref="GeneID:79982"
                     /db_xref="HGNC:HGNC:25881"
                     /db_xref="MIM:617487"
     exon            1..180
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1187
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /note="isoform 1 is encoded by transcript variant 1; dnaJ
                     homolog subfamily B member 14; DnaJ (Hsp40) homolog,
                     subfamily B, member 14"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 14 isoform 1"
                     /protein_id="NP_001026893.1"
                     /db_xref="CCDS:CCDS34035.1"
                     /db_xref="GeneID:79982"
                     /db_xref="HGNC:HGNC:25881"
                     /db_xref="MIM:617487"
                     /translation="MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSAR
                     ALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLS
                     INKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLS
                     NPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSF
                     SNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYS
                     LYPRSGTGQTIKMQTENLGVVYYVNKDFKNEYKGMLLQKVEKSVEEDYVTNIRNNCWK
                     ERQQKTDMQYAAKVYRDDRLRRKADALSMDNCKELERLTSLYKGG"
     exon            181..352
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            353..498
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            499..684
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            685..779
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            780..889
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1062
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..5967
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2644..2649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /note="hexamer: AATAAA"
     polyA_site      2673
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /note="major polyA site"
     regulatory      5940..5945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
                     /note="hexamer: AATAAA"
     polyA_site      5967
                     /gene="DNAJB14"
                     /gene_synonym="EGNR9427; PRO34683"
ORIGIN      
        1 gcccttctta gctgcgctgc cggaggaaac ggaagaagga gcaagctatg gaggggaaca
       61 gggatgaggc tgagaaatgt gtcgagatcg cccgggaggc cctgaacgcc ggcaaccgcg
      121 agaaggccca gcgcttcctg cagaaggccg agaagctcta cccactgccc tcggcccgcg
      181 cactattgga aataattatg aaaaatggaa gcacggctgg aaatagccct cattgccgaa
      241 aaccatcagg tagtggcgat caaagcaagc ctaattgcac aaaggacagc acatctggta
      301 gtggtgaagg tggaaaaggc tataccaaag accaagtaga tggagttctc agcataaaca
      361 aatgtaaaaa ttactatgaa gtacttggag ttacgaaaga tgctggtgat gaagatttga
      421 aaaaagctta tagaaagctt gctttgaagt ttcatccaga caaaaaccat gcacctggag
      481 caacagatgc ttttaaaaag attggaaatg cttatgctgt tttaagtaat ccagaaaagc
      541 gaaaacagta tgacctcacg ggcaatgaag aacaagcatg taaccaccaa aacaatggca
      601 gatttaattt ccatagaggt tgtgaagctg atataactcc agaagacttg tttaatatat
      661 tttttggggg tggatttcct tcaggtagtg tacattcttt ttcaaatgga agagctggtt
      721 atagccaaca acatcagcat cgacatagtg gacatgaaag agaagaggaa agaggagatg
      781 gaggtttttc tgtgtttatc cagctgatgc ccataattgt attgatcctc gtgtcattat
      841 taagccagtt gatggtctct aatcctcctt attccttata tcccagatct ggaactgggc
      901 aaactattaa aatgcaaaca gaaaacttgg gtgttgttta ttatgtcaac aaggacttca
      961 aaaatgaata taaaggaatg ttattacaaa aggtagaaaa gagtgtggag gaagattatg
     1021 tgactaatat tcgaaataac tgctggaaag aaagacaaca aaaaacagat atgcagtatg
     1081 cagcaaaagt ataccgtgat gatcgactcc gaaggaaggc agatgccttg agcatggaca
     1141 actgtaaaga attagagcgg cttaccagtc tttataaagg aggatgaact ggaattttta
     1201 tttatacctt ttagcgtact ctttattttt tctgtaagta agtttggttt catcatgagg
     1261 gatgaaggaa aagatttgat actgaaaact aaactgaata gttggttcct gaaatcttgg
     1321 actgtttatg acctactggc tcctttaaat agtaactgaa aactaaaatg gaatatttta
     1381 gttaacgctt ctacaagtat tttcatttta aaagcttaca tgattcctaa ctaaagtgtc
     1441 atgagaaagg attatcacac ctgtagcaat ttccagtttt agtgattctc cattttttcc
     1501 cttgtcatgt aaatatttat ggaatgatca ttttgtgtac atacaggtta ctgctttttt
     1561 atttaaattc ttttagtgtt tagctccatg agacacttca gtttaaattg atggaataaa
     1621 tgttatatga cacatttaca ttttccttat caaggtgtca aatatgtgga ctttaaacaa
     1681 tgaaactttt tcaaaaagaa aaaacaaaaa ctttaacttt gtgtaaaatc ttatagtatt
     1741 atcagcttag agggaattga tatttttaat attgccgtta tattccaaaa tatatattga
     1801 gataaatgaa ctggtgtaga atatcagttt gctatttagt tttatgaatt actatacata
     1861 tacatgcata gaaatgaaat gctatactga taaattttaa agaaaatatg aggaaatggc
     1921 tataaatatt aaactaaaag ggtcttcaac agtaaagtgc agttatgtca tttaaaattc
     1981 caatacttta aaggccacca aattttgatg tatatgtcct tgaagggctg ctaaaattta
     2041 tgaagaggac tcacattttc ccccatagaa atttgcagtt tcttggtgat catttaagca
     2101 ggatccaaag aagttccttt acaaataagt aataagaaaa atgagtacta aaatacagct
     2161 ttgtgccttt taaccctatg ccaactccta aacatataag tagattacag tatacttatc
     2221 tgatcagagc atgatctgtt tggccacatg caagtgtgag cagaaataga gcagcacgta
     2281 gaatagtaac ttaaagcaag tcatccttta aaaattctga gctaaaatct atttaccatt
     2341 gagtaattga attaatccca taggaataag ctccttgtaa gtaaatccat gatatgaatt
     2401 agaaaaaaaa aacagctgga aattgaagtt tttgatgcct gtatactgga tatgaaacta
     2461 tttgatttct agtcttctgt gtttagcagt tgtaatattt taatgatttt gcttcatact
     2521 cggttaatgg aacataaaca tatctttgat acttctttgt gagtgagaga atgctagata
     2581 gggtggcttt gttctttgtt taagtttttt ttcctgaatg tagttaattt atggcatctg
     2641 ttgaataaaa ctgctaaaat gacctcttaa aaatgttctg ttgtatcccc ttttccaggt
     2701 gaatcaatag aaatgcctga ttgaattagt aggttaaact aaacaacata ctgtcatagg
     2761 aaaactggag agcttaacca acttgctctt agaaatgtta ccttaaaaaa aaaaaaaaag
     2821 cagtgcttaa gagaaaatac aattttaatt attttgttga gcctgcagag taagttttta
     2881 aaaatataag ttcatagcat tttaattaac tttgaaagtt catatgcatc agaaaattta
     2941 tgaaaatttg aatgaaaaaa atttcatcta tttatttttc taattttaat ggcaaattta
     3001 cactattatg gctgataatt ctgtgaactt accttcttgt tgactgattc tttttccctt
     3061 aatcccagct ttaaggagat aggtgaagtt attgtacaaa gttaagtgat accataaagt
     3121 atatattata aagtcataca tggcttttgg acagtattat atttcagttg cagtgctgca
     3181 ttccattaaa tttcataaaa tgctagggaa aatgtgtttg ataaaatttt ctgcagtgag
     3241 aaatgacaga ctgagtgcct gacaatttaa gccacatatg aagtatgcaa gtaaagattc
     3301 agtccttaat gtcatctata tcatggtata aaagctcaac aagtatctta taatttggag
     3361 ttaactttat aaagtaagaa gagtttggat atcttgataa aagattttca tgtttgctgc
     3421 tttttttata ataaaaatcc caacattttg ccatcttatg tttagctaca tgaacttcgc
     3481 aatttcatcg gctcattcta atgaccacct aaaagtcctc aatctttgaa tattgcttta
     3541 ttagctcctc taactggtat tctaacagtt tagcagcact tatatttatt aatgctttac
     3601 atttttaccc ctttttgtgt aaacacaaat acacccatgc atatacacac aaacattatt
     3661 gtccttgtga gggtttgggg gaaatgaaaa atagtacctt tctcctcttc ttttgattcc
     3721 ttagttccca aatagactgg tgctagtcat ttagtcatct gtaaatttcg aaatgtagtt
     3781 tgattaaatg tgaactgaat tctcagcaat gaaaaatgtc aagttgaatt catctaataa
     3841 gtgaaaataa tatattttgt aaacagatta catttgaaca cctaaataag tatttgtttc
     3901 ataatcatta catgcttgtt tatgatttac aaagatttgg tagagaaaag tacagtcctt
     3961 aaggcatata tatgccaatg cattaaacta ctcagctttt gtgccagctc aggtgttcat
     4021 aggaacagga atgtggaata ccagcttttt actttaatta tacttttatg ctgaattttt
     4081 cttccagtta aacctttaat tacactagta tgtaaagtag ttactgagaa aaataagttt
     4141 ttgatttccc ttctgttgat ctgtaacatt tttaaatgaa gctatttaac acatgacatg
     4201 ctaatgttac ttaatggatt cctatgtatt tcattaaaaa aaagtctccc acctgtgtta
     4261 agacagctaa atatttcttg atgttggctt gaggaccaag tgagccatct tgccagttaa
     4321 gcaagtttta tatcagagga ccaaatggtt aagaagagaa tttgtacatt tcttgagctt
     4381 ccccatgtga agcttgggtt cagtactatc ctattactat ccatcttcat aatgagtgaa
     4441 gcaaactgaa agggaattca taatcttttt tcttatctga acagaaaaac taatgaaaat
     4501 caacaataaa tctttatttt aattttcatt caacagctga aaaaccatgt ctgttgacca
     4561 cattggacca ttgcctagtt aaaatccata aaattcccta taaaccaatg aaaagaaact
     4621 tgtctggcaa taatttccaa cattttaaat tctaattttt agttacaaag aaaaaggata
     4681 gaatgaggct tttcaaggaa ttcttgctca tcccttcaat tatttggcag gtaaaacagt
     4741 cctagaattc agcttccttt ttggttttat tactattaaa tagatacttt catataaatg
     4801 gaaacacttt tcctcaaaac attaattcaa aacatactcc ttatattaag gattatagca
     4861 ccatattttt tgtatattcc ctatgtgtaa ctttttcctg ttggtatgtg atatttttca
     4921 tttaatatga ctatctgctg gtgttggggc caatagattt gtgaatattc tgtggatgtg
     4981 tctatgtata ggtgtatata tatatatata tatttttttt tttttttccc agctaattac
     5041 ctcaaatgtg tgattgttta atatatacca cactatgttt gcacagagca gggcttccat
     5101 tagcagtgga tatttttatg tctgactttg aaaatcagtc aacacaattt cattggtgtg
     5161 aattatttgt tgctcttttt catattttaa tggagagatt cctattataa ggcccatgct
     5221 tcaaacataa ttatatcttt ctatatccag ccatctggaa atacataggg gtaagaagta
     5281 tttcttgaaa tgtccttgtt tagtaagtta gtaaaaactg ggatgactag ttgccaagat
     5341 gtcttgtaac tgcatagcag atgacacctg gcatggcaag tctcattcac ttcagcacaa
     5401 cactctgtac atttcagtct gatattctac tttgccattt aaggacacca ttaaaatcat
     5461 agagattttc agtgacattg gagtattgta attattactt tcaaaaaatt atttttaata
     5521 ggatgattaa accaatgaga agaaacacac ataaatgata ttttgcagtt tatctgtatt
     5581 aagaaatgct tcttgccaat ttctagtttt agtttttgga ttgctacata agactttctt
     5641 tccatttact ttatgtgttt ttaaaagctg caggtaattt ttaaggtttg atggaattat
     5701 aatggaagcc ttgtataagt taaatgaaat tggtaaagga gagcttactt tgtctataat
     5761 gcattcagga ttaaaaatta gatttcaaaa tgaaatgtct gatttgtaaa atatttaatg
     5821 ttatatcaga cttacataaa attttctgtg agaactcaaa tgcatttgat ttttaatgaa
     5881 tgttctgaat gaaatatgaa tttttgtctt tcagtctctg tagtttatct caatatatta
     5941 ataaaaaggt ctgttctcac tgtaaaa
//
