LOCUS       NM_017626               2935 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B12
            (DNAJB12), transcript variant 2, mRNA.
ACCESSION   NM_017626 XM_940302 XM_944536 XM_944538
VERSION     NM_017626.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Purificacao,A.D.D., Debbas,V., Tanaka,L.Y., Gabriel,G.V.M., Wosniak
            Junior,J., De Bessa,T.C., Garcia-Rosa,S., Laurindo,F.R.M. and
            Oliveira,P.V.S.
  TITLE     DNAJB12 and DNJB14 are non-redundant Hsp40 redox chaperones
            involved in endoplasmic reticulum protein reflux
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (1), 130502 (2024)
   PUBMED   37925033
  REMARK    GeneRIF: DNAJB12 and DNJB14 are non-redundant Hsp40 redox
            chaperones involved in endoplasmic reticulum protein reflux.
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Sopha,P., Ren,H.Y., Grove,D.E. and Cyr,D.M.
  TITLE     Endoplasmic reticulum stress-induced degradation of DNAJB12
            stimulates BOK accumulation and primes cancer cells for apoptosis
  JOURNAL   J Biol Chem 292 (28), 11792-11803 (2017)
   PUBMED   28536268
  REMARK    GeneRIF: Data suggest that endoplasmic reticulum (ER)
            stress-induced apoptosis in hepatoma cells is regulated by highly
            labile and ER-associated BCL-2 family member BOK, which is
            controlled at level of protein stability by ER-associated
            degradation components; DNAJB12 is required in hepatoma cells to
            maintain BOK at low levels and suppress ER stress. (DNAJB12 = DnaJ
            (Hsp40) homolog, subfamily B, member 12)
REFERENCE   3  (bases 1 to 2935)
  AUTHORS   Li,K., Jiang,Q., Bai,X., Yang,Y.F., Ruan,M.Y. and Cai,S.Q.
  TITLE     Tetrameric Assembly of K+ Channels Requires ER-Located Chaperone
            Proteins
  JOURNAL   Mol Cell 65 (1), 52-65 (2017)
   PUBMED   27916661
  REMARK    GeneRIF: ER-located J-protein chaperones were identified as key
            regulators for the iogenesis and physiological function of ERG K+
            channels. They regulate two distinct aspects of K+ channel
            biogenesis, the stabilization and assembly of channel subunits.
REFERENCE   4  (bases 1 to 2935)
  AUTHORS   Goodwin,E.C., Motamedi,N., Lipovsky,A., Fernandez-Busnadiego,R. and
            DiMaio,D.
  TITLE     Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the
            nucleus by membranes associated with a novel nuclear pore structure
  JOURNAL   PLoS One 9 (4), e94322 (2014)
   PUBMED   24732912
  REMARK    GeneRIF: Over-expression of DNAJB12 or DNAJB14 causes the formation
            of elaborate membranous structures within cell nuclei, designated
            DJANGOS for DNAJ-associated nuclear globular structures.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2935)
  AUTHORS   Grove,D.E., Fan,C.Y., Ren,H.Y. and Cyr,D.M.
  TITLE     The endoplasmic reticulum-associated Hsp40 DNAJB12 and Hsc70
            cooperate to facilitate RMA1 E3-dependent degradation of nascent
            CFTRDeltaF508
  JOURNAL   Mol Biol Cell 22 (3), 301-314 (2011)
   PUBMED   21148293
  REMARK    GeneRIF: JB12 cooperates with cytosolic Hsc70 and the ubiquitin
            ligase RMA1 to target CFTR and CFTRDeltaF508 for degradation.
REFERENCE   6  (bases 1 to 2935)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 2935)
  AUTHORS   Yamamoto,Y.H., Kimura,T., Momohara,S., Takeuchi,M., Tani,T.,
            Kimata,Y., Kadokura,H. and Kohno,K.
  TITLE     A novel ER J-protein DNAJB12 accelerates ER-associated degradation
            of membrane proteins including CFTR
  JOURNAL   Cell Struct Funct 35 (2), 107-116 (2010)
   PUBMED   21150129
  REMARK    GeneRIF: DNAJB12 is a novel mammalian ER-localized J-protein that
            plays a vital role in the quality control of membrane proteins
REFERENCE   8  (bases 1 to 2935)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC397260.1, BC064920.1 and AL833358.1.
            
            On Aug 13, 2020 this sequence version replaced NM_017626.6.
            
            Summary: DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40
            family of proteins, which regulate molecular chaperone activity by
            stimulating ATPase activity. DNAJ proteins may have up to 3
            distinct domains: a conserved 70-amino acid J domain, usually at
            the N terminus; a glycine/phenylalanine (G/F)-rich region; and a
            cysteine-rich domain containing 4 motifs resembling a zinc finger
            domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by
            OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 3' UTR compared
            to variant 1. Variants 1, 2 and 4 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1029216.1, BC064920.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000444643.8/ ENSP00000403313.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DC397260.1         313-316
            5-1717              BC064920.1         1-1713
            1718-2935           AL833358.1         1177-2394
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..2935
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /note="DnaJ heat shock protein family (Hsp40) member B12"
                     /db_xref="GeneID:54788"
                     /db_xref="HGNC:HGNC:14891"
                     /db_xref="MIM:608376"
     exon            1..155
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1150
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /note="isoform 1 is encoded by transcript variant 2; dnaJ
                     homolog subfamily B member 12; DnaJ (Hsp40) homolog,
                     subfamily B, member 12"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 12 isoform 1"
                     /protein_id="NP_060096.4"
                     /db_xref="CCDS:CCDS7316.3"
                     /db_xref="GeneID:54788"
                     /db_xref="HGNC:HGNC:14891"
                     /db_xref="MIM:608376"
                     /translation="MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVR
                     ALIESLNQKPQTAGDQPPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAV
                     KRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAV
                     LSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVH
                     VYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSP
                     RPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQ
                     QKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG"
     exon            156..333
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            334..479
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            480..665
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            666..745
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            746..855
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1028
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1180
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..2935
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1270..1275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /note="hexamer: ATTAAA"
     polyA_site      1293
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
     polyA_site      2935
                     /gene="DNAJB12"
                     /gene_synonym="DJ10"
                     /note="major polyA site"
ORIGIN      
        1 ggttccgcgt tctggttccg ccatggaatc caacaaggat gaagctgagc gctgtatcag
       61 catcgccctc aaggccatcc agagcaacca gcccgaccgg gcgctccgct tcctggagaa
      121 ggcacagcgg ctgtatccga cgccgcgagt tcgcgccctg attgagtccc tcaaccagaa
      181 accacagact gccggtgacc aacccccacc cacagacaca acccatgcca cccacaggaa
      241 agcaggtggg accgatgccc cctcggccaa cggtgaagct ggaggagaga gcaccaaagg
      301 ctacactgca gaacaggttg cagctgtgaa aagggtcaag caatgtaaag attactatga
      361 gatcctgggg gtgagcagag gggcctcgga tgaggacctg aagaaggcct accgcagact
      421 ggccctcaaa ttccacccag acaagaacca cgcacctggt gccactgaag ccttcaaagc
      481 cattggcaca gcatatgcgg tactcagcaa cccggagaag aggaagcagt atgaccagtt
      541 cggcgatgac aagagccagg cggcccggca cggccatggg catggggatt tccaccgtgg
      601 ctttgaggcc gacatctccc ctgaagacct cttcaacatg ttctttggcg gcggcttccc
      661 ttctagtaac gtccacgtct acagcaacgg ccgcatgcgc tatacctacc agcaaaggca
      721 ggaccgcagg gacaaccagg gtgatggcgg gctaggggtg tttgtgcagc tgatgcctat
      781 cctcatcctg attctcgtgt cagctctcag ccagctcatg gtctccagtc caccctacag
      841 tctgagtcca agaccgtccg tgggccacat ccacaggcga gtcactgacc acctgggtgt
      901 cgtctactat gtgggagaca ctttctccga agagtacaca ggctccagcc tcaaaacagt
      961 cgagcggaat gtggaagatg attatatcgc caacctccgg aacaactgct ggaaggagaa
     1021 gcagcagaag gaaggcttgc tgtaccgggc acgctacttt ggcgacacag atatgtacca
     1081 cagagcacag aagatgggca cccccagctg cagccgactg tcagaggtgc aggcctccct
     1141 gcatggatag tcctgggcca gccacaccac cgaggtccaa actatgaaat ccctggagaa
     1201 tttttggtga catgcactga gccaaggtga tggactgtat atttgaggaa agacaaacaa
     1261 agaaacaaaa ttaaaatgga attggaggcc gaacgctgca cagctgccct ctctctcacc
     1321 cagtaaatgc agaaagctct taggacagac agaaaacctg ccatggggct gcttccctcc
     1381 ctcgcagggc tggcggaggc tccgcatcgc ctctctccta ggatacagaa accatggcaa
     1441 cgaaagtaga atgtaaaact cgcagcagat atcagtggga agggctgggg gagggggcac
     1501 ccagctgcct ttcctccctc acaggagcca ccaccagcca cctctcagga gaagccagag
     1561 gccctggcca aagataggag gaagaaagag aactctccat agaatgtaat ttatagatgc
     1621 gtgtatatgt atatatatct atttatatgt aaataggcat atatgaagat atatatatat
     1681 atagtctact ttttaaactc tgcagggatt tggttaagtt gttgagctga tttcttccat
     1741 gttttagaaa atgaggcctg tttggggaag gtaccctggt gatgtttttg ctagacatta
     1801 gctgtagctg acagcataag gagagtcgca tcagagcttt tcacagcccc ccgaaacccc
     1861 agcctggttt tgatggcagc agtaatcatg atagtgtgaa cccgcgaagg cctcaggatt
     1921 atggttaacc tggagaggag caggccatgg aggcaggtgc caggtcagta ttgagtcaca
     1981 gtagaggccc cacaaggacc tgcttgggtg agaccacctc tcccctctca gcagcgctgg
     2041 tgggctcttt gtcctggtgc ccaggagccc tccagccccc tgcccacaga agggaaggcc
     2101 tgcagagggg gccaggatct acctaaccat ttccccaccc ctaagtccct cagtgggctt
     2161 tgccttatag gtgccacagg gcacagctcc atcattgcca ctggtttttc ctaagtcatg
     2221 caccaaggac ttcagaaagt tcttcctccg agtcagtcag tggtgccggg accccagggc
     2281 agcctcctcg actcctgaag aaaaggcagt gcccgtggct gcctctgtct tcactcgctg
     2341 ttgatgtgac cgccaggcct cctctcgtac cttggacaaa ggatacacct gaaccagaat
     2401 agttttctta ccctcacatt gaaatgagga ttttgcttaa aacagcactt gccgccagtg
     2461 ggaggaggga ggttttattt attcccacag tttctcctgg gccttctgga atgaccatgt
     2521 tttcctgtgg cggtgggtgt gtcattgcgc ccgccgtctt ccaaagcctt acttccccaa
     2581 gctgccagcc aggaggtgat aaccaccctc ccagagtttc tctcggctct ttttttaaaa
     2641 gtcccccaac aaataggatt cttgctggga aaggaagttc ggtaaggaaa tgtcaaatct
     2701 ggtttttaaa caaggtccaa ccctgcaatg aagcagccac ttcagcccag agagattata
     2761 agaactgatg tggccagagt gcctacccac tgtggccccg gtgctctgcc cccacgcccc
     2821 gaccatctgg tcttggtaga tgtccctaga cgggctgagc catgtgcaat aagaacatag
     2881 atcctaaagg atcctctgga agctgtattt cttgaattag aattctcaaa ttgta
//
