LOCUS       NM_006145               2242 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member B1
            (DNAJB1), transcript variant 1, mRNA.
ACCESSION   NM_006145
VERSION     NM_006145.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Zhao,B., Yang,J., Ran,F., Shi,Y., Yang,L., Duan,Y., Shi,Z., Li,X.,
            Zhang,J., Li,Z. and Wang,J.
  TITLE     CircBIRC6 affects prostate cancer progression by regulating
            miR-574-5p and DNAJB1
  JOURNAL   Cancer Biol Ther 25 (1), 2399363 (2024)
   PUBMED   39258752
  REMARK    GeneRIF: CircBIRC6 affects prostate cancer progression by
            regulating miR-574-5p and DNAJB1.
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Shirani,M., Levin,S., Shebl,B., Requena,D., Finkelstein,T.M.,
            Johnson,D.S., Ng,D., Lalazar,G., Heissel,S., Hojrup,P., Molina,H.,
            de Jong,Y.P., Rice,C.M., Singhi,A.D., Torbenson,M.S., Coffino,P.,
            Lyons,B. and Simon,S.M.
  TITLE     Increased Protein Kinase A Activity Induces Fibrolamellar
            Hepatocellular Carcinoma Features Independent of DNAJB1
  JOURNAL   Cancer Res 84 (16), 2626-2644 (2024)
   PUBMED   38888469
  REMARK    GeneRIF: Increased Protein Kinase A Activity Induces Fibrolamellar
            Hepatocellular Carcinoma Features Independent of DNAJB1.
REFERENCE   3  (bases 1 to 2242)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 2242)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2242)
  AUTHORS   Kim,S.S., Kycia,I., Karski,M., Ma,R.K., Bordt,E.A., Kwan,J.,
            Karki,A., Winter,E., Aktas,R.G., Wu,Y., Emili,A., Bauer,D.E.,
            Sethupathy,P. and Vakili,K.
  TITLE     DNAJB1-PRKACA in HEK293T cells induces LINC00473 overexpression
            that depends on PKA signaling
  JOURNAL   PLoS One 17 (2), e0263829 (2022)
   PUBMED   35167623
  REMARK    GeneRIF: DNAJB1-PRKACA in HEK293T cells induces LINC00473
            overexpression that depends on PKA signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2242)
  AUTHORS   Qian,Y.Q., Patel,D., Hartl,F.U. and McColl,D.J.
  TITLE     Nuclear magnetic resonance solution structure of the human Hsp40
            (HDJ-1) J-domain
  JOURNAL   J Mol Biol 260 (2), 224-235 (1996)
   PUBMED   8764402
REFERENCE   7  (bases 1 to 2242)
  AUTHORS   Freeman,B.C. and Morimoto,R.I.
  TITLE     The human cytosolic molecular chaperones hsp90, hsp70 (hsc70) and
            hdj-1 have distinct roles in recognition of a non-native protein
            and protein refolding
  JOURNAL   EMBO J 15 (12), 2969-2979 (1996)
   PUBMED   8670798
REFERENCE   8  (bases 1 to 2242)
  AUTHORS   Ohtsuka,K.
  TITLE     Cloning of a cDNA for heat-shock protein hsp40, a human homologue
            of bacterial DnaJ
  JOURNAL   Biochem Biophys Res Commun 197 (1), 235-240 (1993)
   PUBMED   8250930
REFERENCE   9  (bases 1 to 2242)
  AUTHORS   Hattori,H., Liu,Y.C., Tohnai,I., Ueda,M., Kaneda,T., Kobayashi,T.,
            Tanabe,K. and Ohtsuka,K.
  TITLE     Intracellular localization and partial amino acid sequence of a
            stress-inducible 40-kDa protein in HeLa cells
  JOURNAL   Cell Struct Funct 17 (1), 77-86 (1992)
   PUBMED   1586970
REFERENCE   10 (bases 1 to 2242)
  AUTHORS   Raabe,T. and Manley,J.L.
  TITLE     A human homologue of the Escherichia coli DnaJ heat-shock protein
  JOURNAL   Nucleic Acids Res 19 (23), 6645 (1991)
   PUBMED   1754405
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB454482.1, D49547.1 and
            BC013157.2.
            
            On Nov 22, 2018 this sequence version replaced NM_006145.2.
            
            Summary: This gene encodes a member of the DnaJ or Hsp40 (heat
            shock protein 40 kD) family of proteins. DNAJ family members are
            characterized by a highly conserved amino acid stretch called the
            'J-domain' and function as one of the two major classes of
            molecular chaperones involved in a wide range of cellular events,
            such as protein folding and oligomeric protein complex assembly.
            The encoded protein is a molecular chaperone that stimulates the
            ATPase activity of Hsp70 heat-shock proteins in order to promote
            protein folding and prevent misfolded protein aggregation.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.231032.1,
                                           SRR1803612.100624.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254322.3/ ENSP00000254322.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DB454482.1         89-96
            9-1414              D49547.1           1-1406
            1415-2242           BC013157.2         1404-2231
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..2242
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /note="DnaJ heat shock protein family (Hsp40) member B1"
                     /db_xref="GeneID:3337"
                     /db_xref="HGNC:HGNC:5270"
                     /db_xref="MIM:604572"
     exon            1..259
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1071
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     radial spoke 16 homolog B; dnaJ homolog subfamily B member
                     1; human DnaJ protein 1; dnaJ protein homolog 1; heat
                     shock 40 kDa protein 1; DnaJ (Hsp40) homolog, subfamily B,
                     member 1"
                     /codon_start=1
                     /product="dnaJ homolog subfamily B member 1 isoform 1"
                     /protein_id="NP_006136.1"
                     /db_xref="CCDS:CCDS12312.1"
                     /db_xref="GeneID:3337"
                     /db_xref="HGNC:HGNC:5270"
                     /db_xref="MIM:604572"
                     /translation="MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEK
                     FKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMF
                     AEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQ
                     DPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKI
                     TFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTL
                     DGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVL
                     EQVLPI"
     exon            260..840
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /inference="alignment:Splign:2.1.0"
     exon            841..2242
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2219..2224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /note="hexamer: ATTAAA"
     polyA_site      2242
                     /gene="DNAJB1"
                     /gene_synonym="Hdj1; Hsp40; HSPF1; RSPH16B; Sis1"
                     /note="major polyA site"
ORIGIN      
        1 ggagccgggg gacggcgaca gcgggtcggc gggccgcagg agggggtcat gggtaaagac
       61 tactaccaga cgttgggcct ggcccgcggc gcgtcggacg aggagatcaa gcgggcctac
      121 cgccgccagg cgctgcgcta ccacccggac aagaacaagg agcccggcgc cgaggagaag
      181 ttcaaggaga tcgctgaggc ctacgacgtg ctcagcgacc cgcgcaagcg cgagatcttc
      241 gaccgctacg gggaggaagg cctaaagggg agtggcccca gtggcggtag cggcggtggt
      301 gccaatggta cctctttcag ctacacattc catggagacc ctcatgccat gtttgctgag
      361 ttcttcggtg gcagaaatcc ctttgacacc ttttttgggc agcggaacgg ggaggaaggc
      421 atggacattg atgacccatt ctctggcttc cctatgggca tgggtggctt caccaacgtg
      481 aactttggcc gctcccgctc tgcccaagag cccgcccgaa agaagcaaga tcccccagtc
      541 acccacgacc ttcgagtctc ccttgaagag atctacagcg gctgtaccaa gaagatgaaa
      601 atctcccaca agcggctaaa ccccgacgga aagagcattc gaaacgaaga caaaatattg
      661 accatcgaag tgaagaaggg gtggaaagaa ggaaccaaaa tcactttccc caaggaagga
      721 gaccagacct ccaacaacat tccagctgat atcgtctttg ttttaaagga caagccccac
      781 aatatcttta agagagatgg ctctgatgtc atttatcctg ccaggatcag cctccgggag
      841 gctctgtgtg gctgcacagt gaacgtcccc actctggacg gcaggacgat acccgtcgta
      901 ttcaaagatg ttatcaggcc tggcatgcgg cgaaaagttc ctggagaagg cctccccctc
      961 cccaaaacac ccgagaaacg tggggacctc attattgagt ttgaagtgat cttccccgaa
     1021 aggattcccc agacatcaag aaccgtactt gagcaggttc ttccaatata gctatctgag
     1081 ctccccaagg actgaccagg gacctttcca gagctcaagg atttctggac ctttctacca
     1141 gttgtggacc atgagagggt gggagggccc agggagggct ttcgtactgc tgaatgtttt
     1201 ccagagcata tattacaatc tttcaaagtc gcacactaga cttcagtggt ttttcgagct
     1261 atagggcatc aggtggtggg aacagcagga aaaggcattc cagtctgccc cactgggtct
     1321 ggcagccctc ccgggatggg cccacatcca cctccagtcc ctggccaggg gtgagaggca
     1381 gaccagcaga tggacttgat ccctctgtgt ctttttgctt ctggctggta gataatgtca
     1441 acctgcagtc ttgattccca gaccctgtac actcctcctt ttctgttgtg tgatcagttt
     1501 gtgctttatt ctgtatttgt ctcccatgtc ttgctcttct cctggagaat tctgtcttct
     1561 ctttggccat ctcaaattga gaacctaaac tattcctgca gaactgcctg gttggcgtcc
     1621 acaagcaata cctctcgttc cagcaggacc aagggagcca gcctccagtg agtgactcca
     1681 gcaagtgcag ccacctctcc cttgatggtc tgggagcctg gcctcagcaa ggggccttcc
     1741 tgacctctgg ctccagtgaa gctgaatgtc ctcactttgt gggtcacact ctttacattt
     1801 ctgtaaggca atcttggcac acgtggggct taccagtggc ccaggtaatt ttttgtttca
     1861 tggactatgg actctttcaa agggatctga tccttttgaa ttttgcacag ccctagatac
     1921 aatccctttt gataaaaggg tctttgcttc tgattacagg agcactgtgg aacgtctgta
     1981 aatatgtttt tataattcca tgtatagttg gtgtacactc aaaacctgtc cccggcagcc
     2041 agtgctctct gtatagggcc ataatggaat tctgaagaaa tcttggggag ggaaggggag
     2101 ttggaacaaa tgtctgttcc ctggaggcca gtccagtgct cagaccttta gactcattgt
     2161 aagttgccac tgccaacatg agaccaaagt gtgtgactag tcaatgaagt gcgacagcat
     2221 taaagactga tgctaaacct ca
//
