LOCUS       NM_001539               2319 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DnaJ heat shock protein family (Hsp40) member A1
            (DNAJA1), transcript variant 1, mRNA.
ACCESSION   NM_001539
VERSION     NM_001539.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Mitchem,M.M., Shrader,C., Abedi,E. and Truman,A.W.
  TITLE     Novel insights into the post-translational modifications of
            Ydj1/DNAJA1 co-chaperones
  JOURNAL   Cell Stress Chaperones 29 (1), 1-9 (2024)
   PUBMED   38309209
  REMARK    GeneRIF: Novel insights into the post-translational modifications
            of Ydj1/DNAJA1 co-chaperones.
            Review article
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wu,J., Yang,Q., Zhu,Y., Xia,T., Yi,L., Wang,J. and Ren,X.
  TITLE     DNAJA1 promotes proliferation and metastasis of breast cancer by
            activating mutant P53/NF-kappaB pathway
  JOURNAL   Pathol Res Pract 252, 154921 (2023)
   PUBMED   37977037
  REMARK    GeneRIF: DNAJA1 promotes proliferation and metastasis of breast
            cancer by activating mutant P53/NF-kappaB pathway.
REFERENCE   3  (bases 1 to 2319)
  AUTHORS   Roth,H.E., De Lima Leite,A., Palermo,N.Y. and Powers,R.
  TITLE     Leveraging the Structure of DNAJA1 to Discover Novel Potential
            Pancreatic Cancer Therapies
  JOURNAL   Biomolecules 12 (10), 1391 (2022)
   PUBMED   36291603
  REMARK    GeneRIF: Leveraging the Structure of DNAJA1 to Discover Novel
            Potential Pancreatic Cancer Therapies.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2319)
  AUTHORS   Cao,M., Wei,C., Zhao,L., Wang,J., Jia,Q., Wang,X., Jin,Q. and
            Deng,T.
  TITLE     DnaJA1/Hsp40 is co-opted by influenza A virus to enhance its viral
            RNA polymerase activity
  JOURNAL   J Virol 88 (24), 14078-14089 (2014)
   PUBMED   25253355
  REMARK    GeneRIF: Therefore, we propose that DnaJA1 is co-opted by the
            influenza A virus to enter the nucleus and to enhance its RNA
            polymerase activity in an Hsp70 cochaperone-independent manner.
REFERENCE   5  (bases 1 to 2319)
  AUTHORS   Stark,J.L., Mehla,K., Chaika,N., Acton,T.B., Xiao,R., Singh,P.K.,
            Montelione,G.T. and Powers,R.
  TITLE     Structure and function of human DnaJ homologue subfamily a member 1
            (DNAJA1) and its relationship to pancreatic cancer
  JOURNAL   Biochemistry 53 (8), 1360-1372 (2014)
   PUBMED   24512202
  REMARK    GeneRIF: structure and function of human DNAJA1 and its
            relationship to pancreatic cancer
REFERENCE   6  (bases 1 to 2319)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
REFERENCE   7  (bases 1 to 2319)
  AUTHORS   Davis,A.R., Alevy,Y.G., Chellaiah,A., Quinn,M.T. and Mohanakumar,T.
  TITLE     Characterization of HDJ-2, a human 40 kD heat shock protein
  JOURNAL   Int J Biochem Cell Biol 30 (11), 1203-1221 (1998)
   PUBMED   9839446
REFERENCE   8  (bases 1 to 2319)
  AUTHORS   Kanazawa,M., Terada,K., Kato,S. and Mori,M.
  TITLE     HSDJ, a human homolog of DnaJ, is farnesylated and is involved in
            protein import into mitochondria
  JOURNAL   J Biochem 121 (5), 890-895 (1997)
   PUBMED   9192730
REFERENCE   9  (bases 1 to 2319)
  AUTHORS   Oh,S., Iwahori,A. and Kato,S.
  TITLE     Human cDNA encoding DnaJ protein homologue
  JOURNAL   Biochim Biophys Acta 1174 (1), 114-116 (1993)
   PUBMED   8334161
REFERENCE   10 (bases 1 to 2319)
  AUTHORS   Chellaiah,A., Davis,A. and Mohanakumar,T.
  TITLE     Cloning of a unique human homologue of the Escherichia coli DNAJ
            heat shock protein
  JOURNAL   Biochim Biophys Acta 1174 (1), 111-113 (1993)
   PUBMED   8334160
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI462555.1, BC008182.1,
            DB575768.1, BU508753.1 and AK095541.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001539.3.
            
            Summary: This gene encodes a member of the DnaJ family of proteins,
            which act as heat shock protein 70 cochaperones. Heat shock
            proteins facilitate protein folding, trafficking, prevention of
            aggregation, and proteolytic degradation. Members of this family
            are characterized by a highly conserved N-terminal J domain, a
            glycine/phenylalanine-rich region, four CxxCxGxG zinc finger
            repeats, and a C-terminal substrate-binding domain. The J domain
            mediates the interaction with heat shock protein 70 to recruit
            substrates and regulate ATP hydrolysis activity. In humans, this
            gene has been implicated in positive regulation of virus
            replication through co-option by the influenza A virus. Several
            pseudogenes of this gene are found on other chromosomes. [provided
            by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.264389.1,
                                           SRR1803616.30216.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330899.5/ ENSP00000369127.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-518               BI462555.1         66-583
            519-1476            BC008182.1         502-1459
            1477-1726           DB575768.1         241-490
            1727-1961           BU508753.1         287-521
            1962-2319           AK095541.1         22-379              c
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p21.1"
     gene            1..2319
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /note="DnaJ heat shock protein family (Hsp40) member A1"
                     /db_xref="GeneID:3301"
                     /db_xref="HGNC:HGNC:5229"
                     /db_xref="MIM:602837"
     exon            1..111
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            112..253
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..1315
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /note="isoform 1 is encoded by transcript variant 1; heat
                     shock protein, DNAJ-like 2; neural precursor cell
                     expressed, developmentally down-regulated 7; dnaJ homolog
                     subfamily A member 1; human DnaJ protein 2; heat shock
                     protein J2; dnaJ protein homolog 2; heat shock 40 kDa
                     protein 4; DnaJ (Hsp40) homolog, subfamily A, member 1"
                     /codon_start=1
                     /product="dnaJ homolog subfamily A member 1 isoform 1"
                     /protein_id="NP_001530.1"
                     /db_xref="CCDS:CCDS6533.1"
                     /db_xref="GeneID:3301"
                     /db_xref="HGNC:HGNC:5229"
                     /db_xref="MIM:602837"
                     /translation="MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEK
                     FKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRER
                     RGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQI
                     RIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKD
                     GQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKP
                     ISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFL
                     SPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGV
                     QCQTS"
     exon            254..431
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            432..536
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            537..764
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            765..879
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            880..995
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1096
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..2319
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1456..1461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /note="hexamer: AATAAA"
     polyA_site      1478
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
     polyA_site      1728
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /note="major polyA site"
     regulatory      2302..2307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
                     /note="hexamer: ATTACA"
     polyA_site      2319
                     /gene="DNAJA1"
                     /gene_synonym="DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2;
                     HSPF4; NEDD7"
ORIGIN      
        1 gcggaacttt ccagaacgct cggtgagagg cggaggagcg gtaactaccc cggctgcgca
       61 cagctcggcg ctccttcccg ctccctcaca caccggcctc agcccgcacc ggcagtagaa
      121 gatggtgaaa gaaacaactt actacgatgt tttgggggtc aaacccaatg ctactcagga
      181 agaattgaaa aaggcttata ggaaactggc tttgaagtac catcctgata agaacccaaa
      241 tgaaggagag aagtttaaac agatttctca agcttacgaa gttctctctg atgcaaagaa
      301 aagggaatta tatgacaaag gaggagaaca ggcaattaaa gagggtggag caggtggcgg
      361 ttttggctcc cccatggaca tctttgatat gttttttgga ggaggaggaa ggatgcagag
      421 agaaaggaga ggtaaaaatg ttgtacatca gctctcagta accctagaag acttatataa
      481 tggtgcaaca agaaaactgg ctctgcaaaa gaatgtgatt tgtgacaaat gtgaaggtag
      541 aggaggtaag aaaggagcag tagagtgctg tcccaattgc cgaggtactg gaatgcaaat
      601 aagaattcat cagataggac ctggaatggt tcagcaaatt cagtctgtgt gcatggagtg
      661 ccagggccat ggggagcgga tcagtcctaa agatagatgt aaaagctgca acggaaggaa
      721 gatagttcga gagaagaaaa ttttagaagt tcatattgac aaaggcatga aagatggcca
      781 gaagataaca ttccatggtg aaggagacca agaaccagga ctggagccag gcgatattat
      841 cattgtgtta gatcagaagg accatgctgt ttttactcga cgaggagaag accttttcat
      901 gtgtatggac atacagctcg ttgaagcact gtgtggcttc cagaagccaa tatctactct
      961 tgacaaccga accatcgtca tcacctctca tccaggtcag attgtcaagc atggagatat
     1021 caagtgtgta ctaaatgaag gcatgccaat ttatcgtaga ccatatgaaa agggtcgcct
     1081 aatcatcgaa tttaaggtaa actttcctga gaatggcttt ctctctcctg ataaactgtc
     1141 tttgctggaa aaactcctac ccgagaggaa ggaagtggaa gagactgatg agatggacca
     1201 agtagaactg gtggactttg atccaaatca ggaaagacgg cgccactaca atggagaagc
     1261 atatgaggat gatgaacatc atcccagagg tggtgttcag tgtcagacct cttaatgggc
     1321 cagtgaataa cactcactgc tggcatttaa tgtgcagtag tgaatgagtg aaggactgta
     1381 atcataatat gctcactact tgctcttgtt tttgttttaa taaactatag tagtgtttta
     1441 aaaagttaaa tgaagaataa acgcaaatat aaaagctctg attttgccct gtatgtatga
     1501 tgacttcagt gtgcaagatg aagtttaata cctgtaaaaa ctacaaagaa gttcccctag
     1561 catttctagg ccaaaccttg taattgactt cagctatgta cgtggacaag cttagactga
     1621 aatgctaggt atatgtattg gcttcagtgt atgacccttc attgttaagc tatgaaagta
     1681 aaactctgta tttaactggc aatgaggaaa aaaaaatttt gtagagaagt gttggtctgt
     1741 atagttcttt atattaagtg ggattcattg taatgcctct gcatttattc tgttgcctca
     1801 gctgttactt gaagatggcg taatatataa tttatcctgt ggtatcagtg ataaaaatga
     1861 tacctttctg taggaggggt ttatcataat atgctgcttc ttgaaggctt gcacttccag
     1921 aattgtgttt ccttctgctg tgccattcat atatatatac atatatatat ataatcttga
     1981 ccagtcctgg tcatttgctc ccctccttgt ctgtggacca tgataagccc aagtagtgac
     2041 ttcagagctg ggtaacagaa attaaagtga aaagaccttt acgtggagaa tttgcatgcg
     2101 taatatagga aggtgttctt taggtatgtt acaggattac tttaaaccat ttgactttcg
     2161 ctccaaagtt atgttggtag tatagcaaat tatgatgaat agctttaatt gtatgtttaa
     2221 aagtctcata tgttcacatg cttaaatctg ggtatcagaa tttaagcaat tcttgaaatg
     2281 tattgtctcc ttaatatact aattacaaag catctccaa
//
