LOCUS       NM_001080449            4267 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript
            variant 1, mRNA.
ACCESSION   NM_001080449 XM_166103
VERSION     NM_001080449.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Mackay,H.L., Stone,H.R., Ronson,G.E., Ellis,K., Lanz,A., Aghabi,Y.,
            Walker,A.K., Starowicz,K., Garvin,A.J., Van Eijk,P., Koestler,S.A.,
            Anthony,E.J., Piberger,A.L., Chauhan,A.S., Conway-Thomas,P.,
            Vaitsiankova,A., Vijayendran,S., Beesley,J.F., Petermann,E.,
            Brown,E.J., Densham,R.M., Reed,S.H., Dobbs,F., Saponaro,M. and
            Morris,J.R.
  TITLE     USP50 suppresses alternative RecQ helicase use and deleterious DNA2
            activity during replication
  JOURNAL   Nat Commun 15 (1), 8102 (2024)
   PUBMED   39284827
  REMARK    GeneRIF: USP50 suppresses alternative RecQ helicase use and
            deleterious DNA2 activity during replication.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Garcia-Rodriguez,N., Dominguez-Garcia,I., Dominguez-Perez,M.D.C.
            and Huertas,P.
  TITLE     EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR
            activation and to maintain viability in BRCA1-deficient cells
  JOURNAL   Nucleic Acids Res 52 (11), 6376-6391 (2024)
   PUBMED   38721777
  REMARK    GeneRIF: EXO1 and DNA2-mediated ssDNA gap expansion is essential
            for ATR activation and to maintain viability in BRCA1-deficient
            cells.
REFERENCE   3  (bases 1 to 4267)
  AUTHORS   Thongon,N., Ma,F., Baran,N., Lockyer,P., Liu,J., Jackson,C.,
            Rose,A., Furudate,K., Wildeman,B., Marchesini,M., Marchica,V.,
            Storti,P., Todaro,G., Ganan-Gomez,I., Adema,V.,
            Rodriguez-Sevilla,J.J., Qing,Y., Ha,M.J., Fonseca,R., Stein,C.,
            Class,C., Tan,L., Attanasio,S., Garcia-Manero,G., Giuliani,N.,
            Berrios Nolasco,D., Santoni,A., Cerchione,C., Bueso-Ramos,C.,
            Konopleva,M., Lorenzi,P., Takahashi,K., Manasanch,E.,
            Sammarelli,G., Kanagal-Shamanna,R., Viale,A., Chesi,M. and Colla,S.
  TITLE     Targeting DNA2 overcomes metabolic reprogramming in multiple
            myeloma
  JOURNAL   Nat Commun 15 (1), 1203 (2024)
   PUBMED   38331987
  REMARK    GeneRIF: Targeting DNA2 overcomes metabolic reprogramming in
            multiple myeloma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4267)
  AUTHORS   Di Lazzaro Filho,R., Yamamoto,G.L., Silva,T.J., Rocha,L.A.,
            Linnenkamp,B.D.W., Castro,M.A.A., Bartholdi,D., Schaller,A.,
            Leeb,T., Kelmann,S., Utagawa,C.Y., Steiner,C.E., Steinmetz,L.,
            Honjo,R.S., Kim,C.A., Wang,L., Abourjaili-Bilodeau,R.,
            Campeau,P.M., Warman,M., Passos-Bueno,M.R., Hoch,N.C. and
            Bertola,D.R.
  TITLE     Biallelic variants in DNA2 cause poikiloderma with congenital
            cataracts and severe growth failure reminiscent of Rothmund-Thomson
            syndrome
  JOURNAL   J Med Genet 60 (11), 1127-1132 (2023)
   PUBMED   37055165
REFERENCE   5  (bases 1 to 4267)
  AUTHORS   Liu,W., Polaczek,P., Roubal,I., Meng,Y., Choe,W.C., Caron,M.C.,
            Sedgeman,C.A., Xi,Y., Liu,C., Wu,Q., Zheng,L., Masson,J.Y., Shen,B.
            and Campbell,J.L.
  TITLE     FANCD2 and RAD51 recombinase directly inhibit DNA2 nuclease at
            stalled replication forks and FANCD2 acts as a novel RAD51 mediator
            in strand exchange to promote genome stability
  JOURNAL   Nucleic Acids Res 51 (17), 9144-9165 (2023)
   PUBMED   37526271
  REMARK    GeneRIF: FANCD2 and RAD51 recombinase directly inhibit DNA2
            nuclease at stalled replication forks and FANCD2 acts as a novel
            RAD51 mediator in strand exchange to promote genome stability.
REFERENCE   6  (bases 1 to 4267)
  AUTHORS   Masuda-Sasa,T., Imamura,O. and Campbell,J.L.
  TITLE     Biochemical analysis of human Dna2
  JOURNAL   Nucleic Acids Res 34 (6), 1865-1875 (2006)
   PUBMED   16595800
  REMARK    GeneRIF: hDna2 showed ATPase/helicase activity and 5'-3'
            exo-endonuclease as well as 3'-5' exo-endonuclease activity,
            indicating that its biochemical properties are very similar to
            yeast DNA2.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4267)
  AUTHORS   Kim,J.H., Kim,H.D., Ryu,G.H., Kim,D.H., Hurwitz,J. and Seo,Y.S.
  TITLE     Isolation of human Dna2 endonuclease and characterization of its
            enzymatic properties
  JOURNAL   Nucleic Acids Res 34 (6), 1854-1864 (2006)
   PUBMED   16595799
  REMARK    GeneRIF: The purified human Dna2 enzyme contains intrinsic
            endonuclease and DNA-dependent ATPase activities, but is devoid of
            detectable DNA helicase activity.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4267)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
REFERENCE   9  (bases 1 to 4267)
  AUTHORS   Bae,S.H., Bae,K.H., Kim,J.A. and Seo,Y.S.
  TITLE     RPA governs endonuclease switching during processing of Okazaki
            fragments in eukaryotes
  JOURNAL   Nature 412 (6845), 456-461 (2001)
   PUBMED   11473323
REFERENCE   10 (bases 1 to 4267)
  AUTHORS   Eki,T., Okumura,K., Shiratori,A., Abe,M., Nogami,M., Taguchi,H.,
            Shibata,T., Murakami,Y. and Hanaoka,F.
  TITLE     Assignment of the closest human homologue (DNA2L:KIAA0083) of the
            yeast Dna2 helicase gene to chromosome band 10q21.3-q22.1
  JOURNAL   Genomics 37 (3), 408-410 (1996)
   PUBMED   8938459
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC404138.1, D42046.1 and
            BC073945.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001080449.2.
            
            Summary: This gene encodes a member of the DNA2/NAM7 helicase
            family. The encoded protein is a conserved helicase/nuclease
            involved in the maintenance of mitochondrial and nuclear DNA
            stability. Mutations in this gene are associated with autosomal
            dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel
            syndrome 8. Alternatively spliced transcript variants have been
            found for this gene. [provided by RefSeq, Sep 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D42046.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000358410.8/ ENSP00000351185.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                DC404138.1         1-58
            59-4131             D42046.1           1-4073
            4132-4267           BC073945.1         1739-1874
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q21.3"
     gene            1..4267
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /note="DNA replication helicase/nuclease 2"
                     /db_xref="GeneID:1763"
                     /db_xref="HGNC:HGNC:2939"
                     /db_xref="MIM:601810"
     exon            1..183
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..3292
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /EC_number="3.6.4.12"
                     /note="DNA2 DNA replication helicase 2-like; DNA
                     replication helicase 2 homolog; DNA2-like helicase; DNA
                     replication ATP-dependent helicase-like homolog"
                     /codon_start=1
                     /product="DNA replication ATP-dependent helicase/nuclease
                     DNA2"
                     /protein_id="NP_001073918.2"
                     /db_xref="CCDS:CCDS44415.2"
                     /db_xref="GeneID:1763"
                     /db_xref="HGNC:HGNC:2939"
                     /db_xref="MIM:601810"
                     /translation="MEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMD
                     NRYLVLAVNTVQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGD
                     CTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGT
                     VLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFC
                     KWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKI
                     DVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLL
                     YLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKT
                     CKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTL
                     ESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIP
                     VTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEE
                     KNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILK
                     GLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAV
                     DNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVA
                     TTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNR
                     EARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANA
                     VINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNV
                     TEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGR
                     DKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLL
                     NHLNSEKLIIDLPSREHESLCHILGDFQRE"
     exon            184..366
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            367..550
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            551..696
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            697..828
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            829..1048
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1166
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1329
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1524
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1755
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1872
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1982
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2092
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2317
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2511
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2601
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2806
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2896
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..3076
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3223
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..4267
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4245..4250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /note="hexamer: AATAAA"
     polyA_site      4267
                     /gene="DNA2"
                     /gene_synonym="DNA2L; hDNA2; RTS4"
                     /note="major polyA site"
ORIGIN      
        1 atgcgcgcga ggtgcgcagg ctcgcgcctt tttccctttt ctaagctttc tgtgttaccc
       61 ccggttccgc tgtcttttct gtctacagtt tgcgatcccc gcgtccagga tggagcagct
      121 gaacgaactg gagctgctga tggagaagag tttttgggag gaggcggagc tgccggcgga
      181 gctatttcag aagaaagtgg tagcttcctt tccaagaaca gttctgagca caggaatgga
      241 taaccggtac ctggtgttgg cagtcaatac tgtacagaac aaagagggaa actgtgaaaa
      301 gcgcctggtc atcactgctt cacagtcact agaaaataaa gaactatgca tccttaggaa
      361 tgactggtgt tctgttccag tagagccagg agatatcatt catttggagg gagactgcac
      421 atctgacact tggataatag ataaagattt tggatatttg attctgtatc cagacatgct
      481 gatttctggc accagcatag ccagtagtat tcgatgtatg agaagagctg tcctgagtga
      541 aacttttagg agctctgatc cagccacacg ccaaatgcta attggtacgg ttctccatga
      601 ggtgtttcaa aaagccataa ataatagctt tgccccagaa aagctacaag aacttgcttt
      661 tcaaacaatt caagaaataa gacatttgaa ggaaatgtac cgcttaaatc taagtcaaga
      721 tgaaataaaa caagaagtag aggactatct tccttcgttt tgtaaatggg caggagattt
      781 catgcataaa aacacttcga ctgacttccc tcagatgcag ctctctctgc caagtgataa
      841 tagtaaggat aattcaacat gtaacattga agtcgtgaaa ccaatggata ttgaagaaag
      901 catttggtcc cctaggtttg gattgaaagg caaaatagat gttacagttg gtgtgaaaat
      961 acatcgaggg tataaaacaa aatacaagat aatgccgctg gaacttaaaa ctggcaaaga
     1021 atcaaattct attgaacacc gtagtcaggt tgttctgtac actctactaa gccaagagag
     1081 aagagctgat ccagaggctg gcttgcttct ctacctcaag actggtcaga tgtaccctgt
     1141 gcctgccaac catctagata aaagagaatt attaaagcta agaaaccaga tggcattctc
     1201 attgtttcac cgtattagca aatctgctac tagacagaag acacagcttg cttctttgcc
     1261 acaaataatt gaggaagaga aaacttgtaa atattgttca caaattggca attgtgctct
     1321 ttatagcaga gcagttgaac aacagatgga ttgtagttca gtcccaattg tgatgctgcc
     1381 caaaatagaa gaagaaaccc agcatctgaa gcaaacacac ttagaatatt tcagcctttg
     1441 gtgtctaatg ttaaccctgg agtcacaatc gaaggataat aaaaagaatc accaaaatat
     1501 ctggctaatg cctgcttcgg aaatggagaa gagtggcagt tgcattggaa acctgattag
     1561 aatggaacat gtaaagatag tttgtgatgg gcaatattta cataatttcc aatgtaaaca
     1621 tggtgccata cctgtcacaa atctaatggc aggtgacaga gttattgtaa gtggagaaga
     1681 aaggtcactg tttgctttgt ctagaggata tgtgaaggag attaacatga caacagtaac
     1741 ttgtttatta gacagaaact tgtcggtcct tccagaatca actttgttca gattagacca
     1801 agaagaaaaa aattgtgata tagatacccc attaggaaat ctttccaaat tgatggaaaa
     1861 cacgtttgtc agcaaaaaac ttcgagattt aattattgac tttcgtgaac ctcagtttat
     1921 atcctacctt agttctgttc ttccacatga tgcaaaggat acagttgcct gcattctaaa
     1981 gggtttgaat aagcctcaga ggcaagcgat gaaaaaggta cttctttcaa aagactacac
     2041 actcatcgtg ggtatgcctg ggacaggaaa aacaactacg atatgtactc tcgtaagaat
     2101 tctctacgcc tgtggtttta gcgttttgtt gaccagctat acacactctg ctgttgacaa
     2161 tattcttttg aagttagcca agtttaaaat aggatttttg cgtttgggtc agattcagaa
     2221 ggttcatcca gctatccagc aatttacaga gcaagaaatt tgcagatcaa agtccattaa
     2281 atccttagct cttctagaag aactctacaa tagtcaactt atagttgcaa caacatgtat
     2341 gggaataaac catccaatat tttcccgtaa aatttttgat ttttgtattg tggatgaagc
     2401 ctctcaaatt agccaaccaa tttgtctggg cccccttttt ttttcacgga gatttgtgtt
     2461 agtgggggac catcagcagc ttcctcccct ggtgctaaac cgtgaagcaa gagctcttgg
     2521 catgagtgaa agcttattca agaggctgga gcagaataag agtgctgttg tacagttaac
     2581 cgtgcagtac agaatgaaca gtaaaattat gtccttaagt aataagctga cctatgaggg
     2641 caagctggag tgtggatcag acaaagtggc caatgcagtg ataaacctac gtcactttaa
     2701 agatgtgaag ctggaactgg aattttatgc tgactattct gataatcctt ggttgatggg
     2761 agtatttgaa cccaacaatc ctgtttgttt ccttaataca gacaaggttc cagcgccaga
     2821 acaagttgaa aaaggtggtg tgagcaatgt aacagaagcc aaactcatag ttttcctaac
     2881 ctccattttt gttaaggctg gatgcagtcc ctctgatatt ggtattattg caccgtacag
     2941 gcagcaatta aagatcatca atgatttatt ggcacgttct attgggatgg tcgaagttaa
     3001 tacagtagac aaataccaag gaagggacaa aagtattgtc ctagtatctt ttgttagaag
     3061 taataaggat ggaactgttg gtgaactctt gaaagattgg cgacgtctta atgttgctat
     3121 aaccagagcc aaacataaac tgattcttct ggggtgtgtg ccctcactaa attgctatcc
     3181 tcctttggag aagctgctta atcatttaaa ctcagaaaaa ttaatcattg atcttccatc
     3241 aagagaacat gaaagtcttt gccacatatt gggtgacttt caaagagaat aaaacactat
     3301 ttcccttgcc ttttcatact agggcagtat ctcctctagc tagtgcccat acagaaaatt
     3361 ctatcaccat acaaaattta atgcagtatt tatgttttaa agcacaggtg taccgaaaac
     3421 tgtgaaaagt ctgaatttat gggttctatg catgcatttt tgcctaacct agagaaagag
     3481 tttgataaat ttttaccagc tttgaagatg gattaacttt tgactttgag ctttaaactt
     3541 ttaagtcaga catttcagga ctaatttgat tttgtagata tcattgtaag aactttattt
     3601 gaaagactga ataaagggat ttgatttgtt ttcatcattt aagcacagtc ttgtgatgat
     3661 gagaacataa gtgtgattct tttctgtatt ttgaggtccc taatccaaag cccattttgc
     3721 taggattttt tctgctatca gatgtgtttt cactctaaac ctagtctttt atgacatgaa
     3781 ttgattactt cctgttaatt ttctatcctc ccttactatc ctcctttttt gttttcagta
     3841 ttcagtattt cagtattcta gagtagattt tgatataaaa gaaaataatt cttacatcat
     3901 cttttgcaac aaattttgtt ttctgaattg ataataaatt taaaaagttg attcctattt
     3961 tcacatatgt tcatatgccc ctatgtttgg gggtatcact cagttttccc ttttttgtgt
     4021 aaagatgttt tgtaaaacaa aattgtctca aagtgattat attatatata taaaaagtaa
     4081 cagattttaa caaaggttaa aagattcttg gggtaacaga ttcttctggg gcttggaaat
     4141 cttccatttc tcttgagggt tttttttaat gagtgttaaa tatgttaaaa tttttatttc
     4201 tacctcatgt gtttttttaa attattactt gaagtttttt atttaataaa ttttttctac
     4261 taatgga
//
